• Journal of Microbiology and Biotechnology
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• v.22 no.4
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• pp.484-493
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• 2012

BolA protein homologs are widely distributed in nature. In this report, we have studied for the first time YrbA, the only BolA homolog present in Escherichia coli, which we have renamed ibaG. We have constructed single and multiple ibaG mutants, and overexpressed ibaG in wild-type strains, in order to characterize this gene. The ibaG phenotypes are different from the bolA-associated round morphologies or growth profiles. Interestingly, ibaG and bolA single-and double-deletion mutants grow faster and have higher viabilities in rich media, whereas the overexpressed strains are significantly growth impaired. However, the mutant strains have lower viabilities than the wild type in the late stationary phase, indicating that both bolA and ibaG are important for survival in difficult growth conditions. bolA, as a transcription factor, binds to some promoters, but ibaG does not interact with the same DNA regions. We have determined that ibaG is transcribed in an operon with the murA gene, involved in the synthesis of peptidoglycan precursors. ibaG was also seen to change its mRNA expression pattern in response to acidic stress. ibaG may thus represent a new gene involved in cell resistance against acid stress.

• Korean Journal of Microbiology
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• v.26 no.4
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• pp.355-361
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• 1988

The aims of the present studies were to understand the physiolosical and genetic characters of Streptomyces sp. isolated from soil. It revealed that Streptomyces sp. SMF301 had very fast growth rate and produced extracellular protease and heavily sporulated on rich media. It also showed $\beta$-lactamase activity and pigment production. Nonsporulating mutants were isolated after NTG or acriflavin treatment and their characters were compared with the parent strain. It was found that the mutants obtained by acriflavin treatment and ghier characters were compared with the parent strain. It was found that the mutants obtained by acriflavin treatment lost the pigment formation and $\beta$-lactamase production. Protease actibity of the mutant was lowered and the pH optimum was changed toward neutral. It was found that the changes were resulted from the reduction of alkaline protease biosynthesis in the bald mutant. Therefore it is considered that sporulation, pigment formation, $\beta$-lactamase production, and alkaline protease production in Streptomyces sp. might be controlled with a closely related relationship.

• Journal of KIISE:Computer Systems and Theory
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• v.31 no.9
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• pp.495-502
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• 2004

A rapid growth of the Internet user and rich media content cause an excessive server load and high network traffic, and thus it decreases the quality of service. A solution to this problem is to distribute the content on multiple replicated servers. However, in this circumstance, clients face additional task of selecting the best server to provide proper so${\gamma}$vice which clients request. This paper is intended to propose an adaptive server selection mechanism for a client request based on distance and server load. This will offer a fast and scalable service in response to the increase of massive and realtime multimedia content and contribute to floating a new business model of digital content service.

• Journal of Broadcast Engineering
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• v.8 no.4
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• pp.339-350
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• 2003

The Part 7 of MPEG-21, called Digital Item Adaptation, aims at an interoperable transparent access of multimedia contents in heterogeneous environments. This standard facilitates the development of Universal Multimedia Access (UMA) systems, which adapt the rich multimedia contents to provide user the best possible presentation under the constraints of various terminals and network connections. Content adaptation has two major aspects: one is modality conversion that converts content from one modality (e.g. video) to different modalities (e.g. image) the other is content scaling that changes the titrates (or qualities) of the contents without converting their modalities. At the output of adaptation process, the highly-subjective qualities of adapted contents nay vary widely with respect to point-of-views of different providers and different users. So, user should have some control on the adaptation process. In this paper, we describe two description tools of user characteristics, the presentation priority preference and the modality conversion preference, which allow user to have flexible choices on the qualities and modalities of output contents. We also present a systematic approach to integrate these user preferences into the adaptation process. These description tools are developed in the process of MPEG-21 standardization.

• KSBB Journal
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• v.5 no.1
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• pp.25-35
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• 1990

In the Xanthan gum fermentation by Xanthomonas campestris there are problems of the large energy consumption by long fermentation time, the mass transfer of oxygen and nutrients by high viscous fermentation broth. In this study, the media optimization and the fed batch fermentation were carried out to decrease fermentation time and increase Xanthan gum yield. The $O_2$ uptake rate (OUR) and $CO_2$ evolution rate(CER) which were obtained from the analysis of fermentation exit gas using a gas chromatograph were investigated. As a result, the fermentation time decreased at optimal assimilable nitrogen concentration but increased at poor or rich assimilable nitrogen concentration, the Xanthan gum biosynthesis was stimulated under the limited condition of assimilable nitrogen source and the optimum fermentation medium was obtained as follow; Glucose=30g / l, Peptone=8.0g / l, $K_2HPO_4=2.0g/l$, $MgS0_47H_2O=10g/l$, Sodium acetate=20g/l, Sodium pyruvate=0.5g/1. As the agitation speed and nitrogen concentration increased, the $O_2$ uptake rate and $CO_2$ evolution rate increased. The OUR and CER were 37.3mmol $O_2/\;l$ hr and 20.2 mmol $CO_2/\;L$ hr at peptone 11g / l and agitation speed 990RPM, respectively. In fed batch fermentation, the final concentration of Xanthan gum was enhanced up to 29g / l.

• Proceedings of the Korean Society of Broadcast Engineers Conference
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• 2019.06a
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• pp.222-224
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• 2019

Light Field (LF) camera captures not only the light intensity but also the light direction coming to camera. While the rich information captured by LF camera enables many interesting applications such as digital refocusing, viewpoint changing, and 3D reconstruction, but it also requires powerful coding tools to reduce its large volume of data. In this paper, we investigate using the intra prediction scheme of the versatile video coding (VVC), which is the most recent video coding technology currently under developing, to compress the LF image. The Intra Block Copy (IBC) technique in VVC is exploited considering special LF image structure. The experimental result shows that VVC intra predict ion outperforms the H.265/HEVC intra coding technique in encoding LF data irrespective of using the IBC mode or not.

• Bulletin of the Korean Chemical Society
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• v.9 no.5
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• pp.317-322
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• 1988

Premicellar precipitation, resolubilization and luminescing behaviors of $RuL_3^{2+}$ (L = bpy, phen, $Me_2bpy$) in aqueous alkylsulfate and sulfonate solutions were studied. Addition of the anionic surfactants to $RuL_3\;^{2+}$ solutions caused initial precipitation which was redissolved by further addition of the surfactants. The apparent solubility products $K_{sp}$'s of the precipitates were evaluated assuming 1:2 salt formation. The values were smaller as the ligand is more hydrophobic and the length of hydrocarbon chain of the surfactant is longer. The $K_{sp}$ values for L = bpy were constant over wide surfactant concentration range. However, those for L = $Me_2bpy$ and also for phen, but to less extent, increased with the surfactant concentration. The resolubilization of 1:2 salts was followed by red-shift of emission band and extensive emission quenching above critical concentration of the surfactants. The critical concentration was lower for more hydrophobic surfactant. For L = $Me_2bpy$, the blue-shifted emission band with enhanced emission intensity was observed in intermediate surfactant concentration region. The high ionic strength of media prevented the precipitate formation, but facilitated the red-shift of the emission bands. The results support that the precipitate is dissolved by accretion of surfactant anions to the salts to form water-soluble surfactant-rich $RuL_3$-surfactant anionic species. These species appeared to aggregate cooperatively to produce large clusters which exhibited the red-shifted emission.

• International Journal of Computer Science & Network Security
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• v.23 no.2
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• pp.13-30
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• 2023

Since the COVID-19 vaccine became available, people have been sharing their opinions on social media about getting vaccinated, causing discussions of the vaccine to trend on Twitter alongside certain events, making the website a rich data source. This paper explores people's perceptions regarding the COVID-19 vaccine during certain events and how these events influenced public opinion about the vaccine. The data consisted of tweets sent during seven important events that were gathered within 14 days of the first announcement of each event. These data represent people's reactions to these events without including irrelevant tweets. The study targeted tweets sent in Arabic from users located in Saudi Arabia. The data were classified as positive, negative, or neutral in tone. Four classifiers were used-support vector machine (SVM), naïve Bayes (NB), logistic regression (LOGR), and random forest (RF)-in addition to a deep learning model using BiLSTM. The results showed that the SVM achieved the highest accuracy, at 91%. Overall perceptions about the COVID-19 vaccine were 54% negative, 36% neutral, and 10% positive.

• Food Science of Animal Resources
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• v.29 no.6
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• pp.702-708
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• 2009

We investigated biofilm formation in various media, growth in low pH, and the hemolytic activity of 14 strains of Cronobacter spp. (Enterobacter sakazakii) isolated from a variety of foods including powdered infant formula (n=75), infant cereal (n=100), honey (n=30), and other infant foods (n=100) in Korea. The Cronobacter spp. adhered and formed biofilms on polyethylene, and a greater extent of biofilm was observed in nutrient-rich media. No clear difference in biofilm-forming ability was noted among the media constituents and the pattern of biofilm formation was strain-dependent. Seven strains out of 14 strains (50%) grew at pH 4.1, indicating that the acid resistance of these Cronobacter spp. isolated in Korea was relatively low. Hemolytic activity was not observed in any of the strains. This study provides basic information for the physiological and biochemical characteristics of Cronobacter spp. isolated from a variety of infant foods in Korea.

• Journal of the Korean Society of Floral Art and Design
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• no.44
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• pp.75-100
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• 2021

The purpose of this study is to explore ways in which the flower design industry can utilize podcasts that are rapidly growing in recent years. I selected foreign flower podcasts that are ranked on the global podcast chart, and examined the genre, content, components, show hosts and etc. By analyzing the characteristics of the podcast, the type of communication between the host and the audience, the audience interaction, the industry connection, and the media expansion strategy, I tried to derive the possibility of the flower podcast in Korea. As a result of analyzing foreign flower podcasts, podcasters built listener communities based on their rich experience and knowledge through podcasts and used them for education and marketing. They acted as leaders in the industry or led public opinion such as the sustainable flower industry. Podcast shows were repurposed as various content and used to spread flower design culture. In Korea, flower podcasts can be the basis for the formation of a community related to the flower design industry. Flower design experts can use podcasts as a source asset for various content. Listeners within the industry can get hands-on knowledge about the business from flower podcasts. The popular flower podcast will contribute to the vitalization of flower design culture and industry. Flower podcasts can be a starting point to actively cope with the era of personal media.