• 한국자원식물학회지
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• 제19권5호
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• pp.612-616
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• 2006

최근 GMO 작물의 재배, 생산이 날로 늘어나며 GMO 작물이 환경에 미칠 수 있는 많은 가능성들이 대두되고 있다. 특히 GMO 작물과 야생종과의 자연교잡에 의한 유전자 전이로, 잡초화의 문제점이 제기되며 생태계의 변화 및 파괴의 위험성이 우려되고 있다. 본 실험에서는 GM벼와 야생 및 근연종 사이의 교잡가능성 및 유전자 전이율을 조사하기 위한 유전자 이동의 분석 체계를 확립하고자 하였다. 벼의 개화시기에 GM벼와 야생 및 근연종 간의 인공교배 후 수확한 교잡 추정 종자를 발아시켜서 제초제를 처리하여 교잡종자를 선별하였다. 또한 GM 벼 및 야생 근연종벼들 간의 RAPD PCR 분석을 통해 선별한 marker를 사용하여 낙동 교잡벼와 샤레 교잡벼가 GM 벼와 교배된 식물체임을 확인하였다. PCR 분석을 수행한 결과 GM벼에서 도입된 trehalose-6-phosphate phosphatase (TPP) 유전자와 선별marker로 사용된 bar유전자가 GM벼 뿐만 아니라 샤레 교잡벼에도 존재하였으며, 결과적으로 GM벼의 bar 및 tpp 유전자가 잡초성벼인 샤레 교잡벼에 전이되었음을 검증할 수 있었다.

• 농업과학연구
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• 제37권2호
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• pp.171-175
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• 2010

This study was carried out to find the effect on growing media and proper time for water supply in rice seedling stages. Plant height of rice seedling for SIPAN-BRL among current several growing medias was higher in Chuchungbyeo than Ilpumbyeo, and that of root length was higher in check among used growing medias. Fresh and dry weight of shoot and root of two rice cultivar. Water time to absorption on each treatment of growing media concentration took similarly in SIPAN-BNH and check were similar. But 500 minutes did in 100ppm and did 370 and 470minute in 300 and 500ppm, respectively. Wilting time of rice seedling was appeared highly at 300ppm of SIPAN-BRL in Chuchungbyeo and 100ppm of SIPAN-BRL in Ilpumbyeo, while it prolonged at 300ppm in Chuchungbyeo and 500ppm in Ilpumbyeo, respectively. Water content at wilting stage was appeared highly at SIPAN-BRL 500ppm in both Chuchungbyo and Ilpumbyeo, while SIPAN-BNL was high in 300ppm of Chuchung-byeo and Ilpumbyeo, respectively. Plant and root length of SIPAN-BNL treated with 300ppm in 10day-rice seedling were highly appeared, while dry weight of shoot was high at 300ppm SIPAN-BNL.

• Plant Resources
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• 제8권1호
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• pp.32-35
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• 2005

This study was conducted to investigate the influence of $K_{2}O$ fertilizer application on growth, yield and lodging resistance of rice. Culm length and panicle length were lowest at the treatment of fertilizer level, $N-P_{2}O_{5}-K_{2}O=16-8-8$ plot but number of panicle, percentage of fruitful culm, $1{\ell}$ grain weights of paddy rice and brown rice, 1,000 grains weight of brown rice, brown rice ratio, yields of paddy rice and brown rice were highest at the treatment of fertilizer level, $N-P_{2}O_{5}-K_{2}O=16-8-8$ kg/10a plot. Bending moment, breaking strength and lodging index were lowest at the treatment of fertilizer level, $N-P_{2}O_{5}-K_{2}O=16-8-8$ kg/10a plot. Judging from the results reported above, an optimum fertilizer level of rice for lodging resistance is most likely be $N-P_{2}O_{5}-K_{2}O=16-8-8$ kg/10a treatment.

• Journal of Crop Science and Biotechnology
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• 제10권2호
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• pp.79-85
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• 2007

Rice bran application just after transplanting has been increasingly practiced as an herbicide-substitute for organic rice production in Korea. However, this practice is frequently reported to be unsatisfactory in weed suppression. An experiment with five treatments that combines flooding depth, rice bran application dose, and herbicide treatment was done in the paddy field to evaluate whether rice bran application under deep flooding can lead to a successful weed control in compensation for the single practice of rice bran application. Rice bran was broadcasted on the flood water surface just after deep flooding of 8 to 10cm that was started at seven days after transplanting. In the shallow flooding plot without herbicide six weed species were recorded: Monochoria vaginalis, Echinochloa crus-galli, Ludvigia prostrate, Cyperus amuricus, Aneima keisak, and Bidens tripartite. Among the first four dominant weed species, deep flooding significantly suppressed the occurrence of Echinochloa crus-galli and Cyperus amuricus while did not suppress the occurrence of Monochoria vaginalis and Ludwigia prostrate. On the contrary, rice bran application under deep flooding suppressed significantly Monochoria vaginalis and Ludwigia prostrate while didn't exert an additional suppression of Echinochloa crus-galli and Cyperus amuricus compared to deep flooding alone. Rice bran application and deep flooding suppressed complimentarily all the six weed species to a satisfactory extent except for Monochoria vaginalis of which suppression efficacy was 31.9%. Deep flooding reduced the panicle number substantially by inhibiting the tiller production, increased the spikelet number per panicle slightly, and leaded to a lower rice grain yield compared to shallow flooding with herbicide. Rice bran application under deep flooding mitigated the panicle reduction due to deep flooding, increased the spikelets per panicle significantly, and thus produced even higher grain yield in the rice bran application of 2000kg $ha^{-1}$ as compared to the shallow flooding treatment with herbicide. In conclusion, this practice applying rice bran under deep flooding would be promising to be incorporated as an integral practice for an organic rice farming system.

• The Plant Pathology Journal
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• 제30권4호
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• pp.343-354
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• 2014

Rice blast disease caused by Magnaporthe oryzae is one of the most serious diseases of cultivated rice (Oryza sativa L.) in most rice-growing regions of the world. In order to investigate early response genes in rice, we utilized the transcriptome analysis approach using a 300 K tilling microarray to rice leaves infected with compatible and incompatible M. oryzae strains. Prior to the microarray experiment, total RNA was validated by measuring the differential expression of rice defense-related marker genes (chitinase 2, barwin, PBZ1, and PR-10) by RT-PCR, and phytoalexins (sakuranetin and momilactone A) with HPLC. Microarray analysis revealed that 231 genes were up-regulated (>2 fold change, p < 0.05) in the incompatible interaction compared to the compatible one. Highly expressed genes were functionally characterized into metabolic processes and oxidation-reduction categories. The oxidative stress response was induced in both early and later infection stages. Biotic stress overview from MapMan analysis revealed that the phytohormone ethylene as well as signaling molecules jasmonic acid and salicylic acid is important for defense gene regulation. WRKY and Myb transcription factors were also involved in signal transduction processes. Additionally, receptor-like kinases were more likely associated with the defense response, and their expression patterns were validated by RT-PCR. Our results suggest that candidate genes, including receptor-like protein kinases, may play a key role in disease resistance against M. oryzae attack.

• The Plant Pathology Journal
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• 제27권1호
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• pp.37-43
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• 2011

Rice stripe virus (RSV) is one of serious epidemic pathogens for rice species grown in many Asian countries. Therefore, it is necessary to produce a diagnostic detection kit applicable in fields for RSV detection. In this study, RSV proteins that were derived from recombinant proteins and synthetic polypeptides as antigens were generated and were raised in rabbits for antiserum production. Among seven proteins in RSV, genes that code for NCP and NS3 proteins were cloned and subcloned into vector carrying His-tag protein and were expressed in E. coli. Of two recombinant proteins, only anti-NCP displayed stable hybridization signals in western blot analysis. Alternately, synthetic RSV polypeptides for CP, NCP, NS3 and NSvc4 we also generated and only antibodies against CP and NCP were very effective to detect RSV in both RSV infected rice and weed plants. However, antibodies against NS3 and NSvc4 showed weak specific bands as well as strong non-specific background due to the difference of viral proteins produced in the infected leaves. In summary, the antibodies generated against RSV proteins produced in this study will be useful for various assays such as for RSV diagnostic detection, immunoprecipitation, protein purification, and western blot analysis.

• The Plant Pathology Journal
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• 제21권4호
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• pp.402-405
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• 2005

We have previously identified genes for four different protease inhibitors (PIs) that were induced upon rice blast infection in a rice blast resistant mutant SHM-11. Our expression analysis of the PIs indicated that induction of the PIs was the highest 24 hr after rice blast inoculation in the rice mutant SHM-11. Three PIs in the group of serine PIs were highly expressed while a cystein PI was weakly expressed upon rice blast inoculation. Four PIs were weakly induced 48 hr after pathogen inoculation in rice blast susceptible wild type rice plant. The simultaneous expression of three serine PIs was apparent from SHM-11 and two of them were induced in rice blast resistant Taebaegbyeo. One of them was induced in rice blast resistant Hwayeongbyeo while none of them were expressed in rice blast susceptible Nagdongbyeo and rice blast resistant Dongjinbyeo. Our results suggest that the expression of PI gene is rice cultivar specific and may be linked with the rice blast resistance in a specific rice mutant by the simultaneous expression of the PI genes.

• The Plant Pathology Journal
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• 제27권4호
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• pp.360-366
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• 2011

Bacterial leaf spot of Euphorbia pulcherrima has been reported in many countries. Characterization by polyphasic approaches indicated that the isolates from India, USA and New Zealand could be distinguished based on rep-PCR profiles and gyrB phylogenies, while the Chinese isolates should be ascribed to Xanthomonas axonopodis pv. poinsettiicola.

• The Plant Pathology Journal
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• 제24권1호
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• pp.58-62
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• 2008

Utilization of carbon sources by Bacillus cereus D324, a biological control agent, and Pythium species, which causes rice seedling disease, was studied with the objective of increasing the efficacy of biological control by providing the biological control agent with specific beneficial carbon sources. D-galactose, D-sorbitol, and D-mannitol were poor carbon sources for Pythium spp. growth but were good for B. cereus D324 growth. Growth in a growth chamber of rice seeds coated with B. cereus D324 amended with specific carbon sources, such as D-galactose and D-sorbitol, showed significantly enhanced seedling emergence compared to seeds coated only with B. cereus D324. Field trials showed that both seedling emergence and yield increased, when the above specific carbon sources were added to B. cereus D324 in seed coating formulations. This result indicated that amending seed coating formulations with specific carbon sources could significantly increase seedling emergence and yield in the field.

• 한국농공학회논문집
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• 제46권1호
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• pp.75-86
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• 2004

The objective of the research is to develop agricultural resue technologies of reclaiming the effluents from a municipal wastewater treatment plant and reusing for irrigated rice paddies. The Suwon wastewater treatment plant was selected for wastewater reuse tests. The control was the plots with groundwater irrigation (TR＃1), the treatment (TR＃2) using polluted stream water as it was, and three others using wastewater after treatment. Three levels of wastewater treatments were employed： the effluent from the wastewater treatment plant (TR＃3), sand filtering after treatment plant(TR＃4), and ultra-violet treatment after sand filtering (TR＃5). The randomized block method was applied to wastewater application to paddy rice with five treatments and six replica. The effects of various wastewater treatment levels on water quality, paddy soil, crop growth, yields, and the health hazards were investigated. The primary results indicate that cultivating rice with reclaimed wastewater irrigation did not cause a problem to adverse effects on crop growth and yields. Overall, wastewater could be used as a practical alternative measure for reclaimed wastewater irrigation. However, long-term monitoring is recommended on the effects on soil chemical characteristics and its related health concerns.