• 제목/요약/키워드: Rice (Oryza sativa)

검색결과 837건 처리시간 0.037초

잡초성벼의 superoxide dismutase cDNA cloning과 재배벼로의 형질전환 (Isolation of Superoxide Dismutase cDNAS from an Weedy Rice Variety and Transformation of a Cultivated Rice Variety)

  • 박상규;박종석;이승인;서석철;김병극;조윤래;서학수
    • 한국환경농학회지
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    • 제21권2호
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    • pp.156-161
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    • 2002
  • 냉해나 한발등의 환경스트레스에 대해 저항성을 유발하는 유전자를 환경스트레스에 강한 잡초성벼로부터 선발하고 이들 유전자를 재배벼에 도입하여 도입유전자 산물의 과량 발현을 통해 냉해나 한발 등에 대한 저항성이 향상된 벼를 선발하고자 하였다. 잡초성벼인 Bhutan 14Ad로부터 냉해 및 한발 저항성 유전자로 알려진 superoxide dismutase (SOD) cDNA를 분리하고자 mRNA를 분리하고 이 분리된 mRNA를 이용해 reverse transcriptase PCR방법으로 SOD cDNA를 cloning 하였다. 그 결과 2종의 SOD cDNA가 cloning되어 SOD-A, SOD-B로 명명하였다. 이들 cDNA의 염기서열을 결정한 결과 이들은 아미노산 서열 상동성이 88.4%를 나타내었으며, SOD-A는 Oryza sativa 계열의 Cu/Zn SOD유전자인 GenBank accession No. L36320와 99.3% 동일하였으며, SOD-B는 accession No. D01000과 100% 동일하였다. 이들 SOD-A와 SOD-B cDNA를 재배벼인 낙동벼에 형질전환하여 형질전환체 벼를 선발하였으며, 이들 형질전환체 벼의 냉해저항성및 한발저항성 검정을 통해 저항성이 향상된 형질전환체 벼를 선발하고 있다.

Mapping of grain alkali digestion trait using a Cheongcheong/Nagdong doubled haploid population in rice

  • Kim, Hak Yoon;Kim, Kyung-Min
    • Journal of Plant Biotechnology
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    • 제43권1호
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    • pp.76-81
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    • 2016
  • We performed a molecular marker-based analysis of quantitative trait loci for traits that determine the quality of appearance of grains using 120 doubled haploid lines developed by anther culture from the F1 cross between 'Cheongcheong' (Oryza sativa L. ssp. Indica) and 'Nagdong' (Oryza sativa L. ssp. Japonica). We therefore calculated the alkali digestion value (ADV), used to indirectly measure gelatinization temperature, to evaluate the quality of cooked rice in 2013 and 2014. The ADV score of frequency distribution was higher milled rice than brown rice. In total, nine different quantitative trait loci (QTLs) were found on 5 chromosomes in 2013 and 2014. Also, chromosome 5, 8 were detected over two years. We conclude that selected molecular markers from this QTL analysis could be exploited in future rice quality. In conclusion, we investigated ADV of brown and milled rice in CNDH population. This study found nine QTLs related to the ADV of brown and milled rice. The detected one marker can be used to select lines with desirable eating-quality traits because ADV is closely associated with the eating quality of cooked rice. Therefore, it will be useful to collect resources and distinguishable in many varieties for rice breeding program.

벼에서 채집한 날개응애류 (1) (Oribatid Mites (Acari: Oribatida) Collected from Rice(Oryza sativa L) 1.)

  • 최성식
    • 한국응용곤충학회지
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    • 제34권2호
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    • pp.132-139
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    • 1995
  • 본 연구는 벼의 줄기, 잎과 벼포기에서 채집한 날개응애류를 분류한 결과로 12종이 동정되었다. 그 등애들은 가시배흠응애, 넓은지게응애, 털나비응애 등의 기지종 3종과 밭소매응애(Protoribates agricola), 머리띠팔자기게응애(Scheloribates decarinatus), 벼팔자지게응애(Schelorlbates oryzae). 이리잔날개응애(Sacculozetes filosus), 참사다리응애(Punctoribates manzanoensis), 덧판지게응애(Paralamellobates ceylanica) 등 6종의 한국미기록종 및 Pelonbates sp., Scheloribates sp, Gaiumma sp. 등이다.

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Morphological Characters of Panicle and Seed Mutants of Rice

  • Kang Sang-Gu;Pandeya Devendra;Kim Sung-Soo;Suh Hak-Soo
    • 한국작물학회지
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    • 제51권4호
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    • pp.348-355
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    • 2006
  • Phenotypes of panicle, hull and seed of mutant rice (Oryza sativa L.) were characterized. Panicle mutants were classified in 4 groups with their internode length of main rachis, primary rachis, secondary rachis and pedicel. Hull and seed mutants were grouped into 12 based on their mutant characters in shape, size and color of seeds. These natural and spontaneous mutant collections showed distinct phenotypes to wild type rice. This might be useful for the identification of the functions of genetic factors in the Mendelian inheritance.