• 약학회지
• /
• 제2권1_2호
• /
• pp.28-33
• /
• 1953

Various types of herbaceous plants are determined. Investigations have been made into the fundamental conception of how their age is be counted. The age list of some kinds of herbaceous plants has been made.

• 한국약용작물학회지
• /
• 제27권6호
• /
• pp.404-411
• /
• 2019

Background: In September 2017, wilting and rhizome rot symptoms were observed on Atractylodes macrocephala Koidz. in Jecheon-si and Eumseong-gun. This study was carried out to isolate hitherto unidentified pathogenic fungi from A. macrocephala and to test the pathogenicity of isolated fungi against Atractylodes spp. genus such as A. macrocephala, A. japonica, and their interspecific hybrids. Methods and Results: The diseased plants were washed with running tap water, and the boundary between the healthy area and the diseased area was cut while the pathogens were isolated by growing cultures from the diseased areas on Phytophthora semi-selective medium. The internal transcribed spacer (ITS) region of the isolates was used in this study for identification. Test plants were cultivated in the glasshouse at 20℃ - 30℃ for 4 months and then used for pathogenicity test. The pots with plants inoculated with mycelial plugs and zoospores were placed at 25℃ for 48 h in a dew chamber where relative humidity was above 95%, and then moved into the glasshouse at 20℃ - 30℃. The presence or absence of pathogenicity of the strains was determined by evaluating the symptom of plant wilting. The inoculation test was performed in three replicates with a non-treated control. Conclusions: On the basis of results of ITS sequence analysis, the strains isolated from the diseased plants was identified as Phytophthora sansomeana. Biological assay using test plants confirmed the pathogenicity of P. sansomeana against Atractylodes macrocephala. This is the first report of rhizome rot in A. macrocephala caused by P. sansomeana.

• 한국자원식물학회지
• /
• 제21권1호
• /
• pp.96-102
• /
• 2008

Nelumbo nucifera Gaertn.(family Nymphaeaceae) has been used for summer heat syndrome as home remedy in Japan, China and Korea. Although whole plant parts are edible, root is commonly consumed. It has been reported that rhizome extract showed anti-diabetic and anti-inflammatory effects. However, in spite of usefulness for treatment of various diseases, the effect of Nelumbo nucifera rhizome(NNR) on proliferation, migration and matrix degrading enzymes-matrix metalloproteinsases(MMPs), the expression of which degrades extracellular matrix(ECM) leading to metastasis, has not been fully elucidated. We examined the effect of hot water extract of NNR on the proliferation, migration and secretion of MMP-2 and MMP-9 in rat smooth muscle cells(rSMC), epidermoid cancer cells(A431) and breast cancer cells(MDA-MB-231). The proliferation assay was carried out using MTT assay, the principle of which depends upon the conversion of MTT by mitochondrial dehydrogensases of viable cells to formazan crystals. The effect of NNR on migration of cells was examined using wound healing assay. Our results showed that there was inhibition in the proliferation, migration and expression of MMP-2 and MMP-9 in dose dependent fashion in all the cells used. Thus, we concluded that NNR could be used as traditional medicine in the treatment of various diseases where proliferation, migration and MMPs' expression plays a pathological role like in restenosis and metastasis.

• 한국약용작물학회지
• /
• 제12권3호
• /
• pp.231-236
• /
• 2004

차광 및 화뢰제거를 통한 Source와 Sink의 인위적 조절이 삽주의 생장 및 수량에 미치는 영향을 조사하여 삽주재배에 관한 정보를 제공하고자 차광율과 화뢰 제거시기를 달리하여 시험을 실시하였던 바 그 결과를 요약하면 다음과 같다. 1. 정식기부터 계속된 차광처리는 생육초기에는 형태보다는 각 부위별 생체중에 주로 영향을 미쳤던 반면, 생육후기인 수확기에서는 차광율이 증가할수록 개체당 경수, 엽수, 근수, 잠아수 및 각부위별 생체중을 감소시켰다. 그러나 일정기간 생장이 이루어진 7월 1일부터 9월 30일까지 35%의 차광처리는 재배기간 내내 차광처리를 하지 않는 무차광과 개체당 생장 및 형태적 형질에는 차이가 없었으나, 단위면적당 수량은 오히려 많은 것으로 조사되었다. 2. 화뢰제거 처리는 처리시기가 빠를수록 개체당 근수와 지상부, 근경 및 뿌리의 생체중을 증가시켰을 뿐만 아니라 단위면적당 수량도 증가시키는 것으로 조사되었다.

• 대한본초학회지
• /
• 제20권4호
• /
• pp.95-101
• /
• 2005

Objectives : In order to distinguish Cnidii Rhizoma and Ligustici Chuanxiong Rhizoma (cultivated in Korea and China), their external and internal states were observed via microscopic examination. Methods : The slice of the tested material made by paraffin section technique was colored with Safranine Malachite Green contrast methods, and then observed and photographed by olymphus-BHT. Results : Cnidii Rhizoma, Ligustici Chuanxiong Rhizoma (from Korea), and Ligustici Chuanxiong Rhizoma (from China) all demonstrated similar patterns as following : 1. external form 1) showing irregular nodular form with $2{\sim}7cm$ diameter. 2) having several trochites in parallel, and nodulose root trace on surface. 3) in addition to yellow-white or yellow-gray intersections, yellow-brown oil sacs were distributed, with cambium resembling a form of annulus. 2. internal form 1) its root and rhizome is composed of 10 layers of cork, with narrow cortical layers. 2) its phloem is wide, and oval light yellow-brown oil sacs are distributed around. The closer to the cambium, the smaller it is. 3) cambium is a form of a wave-like ring or an irregular polygon. 4) xylem and vessel are rare, most of which are arranged in a 'V' format, and sometimes xylem fiber is found. 5) pith is composed of parenchymatous cell, and parenchyma is full of starch grains. Conclusions : In conclusion, C officinale, L. chuanxiong (from Korea), and L. chuanxiong (from China) have rhizomes in a similar form with no special difference. Therefore, it is concluded that C. officinale, L. chuanxiong (from Korea), and L. chuanxiong (from China) are categorized into the same species.

• 생물환경조절학회지
• /
• 제4권2호
• /
• pp.240-245
• /
• 1995

온도 및 광량이 고추냉이의 지상부 및 지하부 생장과 주요 병해발생에 미치는 영향을 조사하기 위하여 생장상의 온도를 10, 17 및 23$^{\circ}C$ 처리구로 하고 차광처리조건을 25, 50 및 75%로 조절하여 실험을 수행하였던 바 그 결과를 요약하면 다음과 같다. 유묘 정식 180일후 조사결과 초장은 생육온도에 따라 차이가 현저히 인정되었으나 17$^{\circ}C$구에서 가장 좋은 결과를 보였으며, 엽병의길이와 직경은 17$^{\circ}C$구를 정점으로 이보다 온도가 높거나 낮음에 따라 감소되는 경향을 보였다. 지하경에 있어서 직경은 1$0^{\circ}C$, 23$^{\circ}C$구간에서는 유의차가 인정되지 않았으며, 17$^{\circ}C$구에서 가장 양호하였다. 길이는 각 처리구별로 유의차가 인정되었으며 역시 17$^{\circ}C$구에서 가장 길었다. 차광처리 75%구가 50, 25%구에 비하여 지하경의 크기는 유의성이 인정되었으나 50%와 25%구의 처리간에는 유의성이 인정되지 않았다. 지하경의 직경과 무게에 있어서 75%구가 기타 처리구에 비하여 양호하였으며 전체 생체중은 75%, 50% 및 25%처리구간에서는 유의차가 인정되었다. 연부병과 묵입병의 발병률은 온도가 증가할수록 증가되었으나 차광량이 많을수록 연부병과 묵입병의 발병율은 감소하는 경향이었다.

• 한국약용작물학회지
• /
• 제7권3호
• /
• pp.200-204
• /
• 1999

토천궁의 줄기마디를 이용한 효율적인 삽목번식 기술을 개발하고자 수행한 시험결과를 요약하면 다음과 같다. 1. 삽목시는 8월30일 $1{\sim}2$마디삽목에서 발근율이 81%로 가장 높았고 발근상태도 양호하였다. 2. 삽수채취에 따른 수량감소는 주당경수의 50%채취에서 9%이하 였으나 100% 채취에서는 $10{\sim}23%$로 비교적 높았다 3. 삽목묘의 포장정식후 활착율은 9월25일 및 10월25일 정식에서 각각 85%, 87%로 높았으나 11월25일 정식에서는 28%로 낮았다. 4. 10a당 수량은 9월25일및, 10월25일 정식에서 각각 127kg, 120kg으로 높았다.

• Journal of Ecology and Environment
• /
• 제33권3호
• /
• pp.261-270
• /
• 2010

Increasing atmospheric $CO_2$ affects the soil carbon cycle by influencing microbial activity and the carbon pool. In this study, the effects of elevated $CO_2$ on extracellular enzyme activities (EEA; ${\beta}$-glucosidase, N-acetylglucosaminidase, aminopeptidase) in salt marsh sediment vegetated with Suaeda japonica were assessed under ambient atmospheric $CO_2$ concentration (380 ppm) or elevated $CO_2$ concentration (760 ppm) conditions. Additionally, the community structure of sulfate-reducing bacteria (SRB) was analyzed via terminal restriction fragments length polymorphism (T-RFLP). Sediment with S. japonica samples were collected from the Hwangsando intertidal flat in May 2005, and placed in small pots (diameter 6 cm, height 10 cm). The pots were incubated for 60 days in a growth chamber under two different $CO_2$ concentration conditions. Sediment samples for all measurements were subdivided into two parts: surface (0-2 cm) and rhizome (4-6 cm) soils. No significant differences were detected in EEA with different $CO_2$ treatments in the surface and rhizome soils. However, the ratio of ${\beta}$-glucosidase activity to N-acetylglucosaminidase activity in rhizome soil was significantly lower (P < 0.01) at 760 ppm $CO_2$ than at 380 ppm $CO_2$, thereby suggesting that the contribution of fungi to the decomposition of soil organic matter might in some cases prove larger than that of bacteria. Community structures of SRB were separated according to different $CO_2$ treatments, suggesting that elevated $CO_2$ may affect the carbon and sulfur cycle in salt marshes.

• Biomolecules & Therapeutics
• /
• 제21권5호
• /
• pp.398-404
• /
• 2013

During a screening program to search the anticolitic herbal medicines, 80% ethanol extract of the rhizome of Anemarrhena asphodeloides (AA) was found to potently inhibit the expression of proinflammatory cytokines TNF-${\alpha}$ and IL-1${\beta}$, as well as the activation of NF-${\kappa}B$ in LPS-stimulated colonic macrophages, followed by that of the rhizome of C. chinensis (CC). AA also potently inhibited TNBS-induced colitic markers, shortening of the colon and increase of macroscopic score, myeloperoxidase activity, TNF-${\alpha}$, IL-1${\beta}$, and IL-6, in mice. The synergistic effect of CC against the anticolitic effect of AA was investigated. CC synergistically inhibited the anticolitic effect of AA. AC-mix (AA+CC, 1:1) potently inhibited them. AC-mix also inhibited the activation of NF-${\kappa}B$, as well as the expression of TNF-${\alpha}$, IL-1${\beta}$, IL-6, iNOS and COX-2. The effects of AC-mix against oxazolone-induced colitis were investigated in mice. AC-mix also potently inhibited oxazolone-induced inflammatory markers, colon shortening, macroscopic score, myeloperoxidase activity, NF-${\kappa}B$ activation and proinflammatory cytokines. Overall, the anti-colitic effect of AC-mix was superior to that of mesalazine. Based on these findings, AC-mix may improve colitis by inhibiting NF-${\kappa}B$ activation.

• 한국환경농학회지
• /
• 제29권2호
• /
• pp.138-145
• /
• 2010

Curcuma longa L. (turmeric) is an important medicinal plant that has been historically used in herbal medicine and in the health food throughout Asia etc. Recently, the demands on rhizome of turmeric are increasing greatly by well-being boom, but there is not enough to meet the demands. To fulfill increasing demands, cultivation system strategies using the organic fertilizers are required to produce a greater amount of rhizome with good quality and yield. A field experiment was conducted to determine the effect of composts, NPK fertilizer(control, F), NPK + swine manure(SM) and NPK + fermentation manure from the wasted oriental medicine materials(OMWM), on rhizome yield and the content of bioactive components for quality. Our results showed that two compost applications can increase both rhizomes(24.1-25.9%) and curcumin(21.7-41.0%) yields, respectively, compared to F control. The content of amino acids increased significantly by SM and OMWM treatments. SM and OMWM application also increased the total phenol yields 7.8 and 8.7 g/10a compared with control 6.3 g/10a, the flavonoid yields 6.3 and 7.3 g/10a compared with control 5.3 g/10a, and also antioxidant activity 21.7 and 41%, respectively, as compared to the control. Especially, OMWM was more effective in total rhizomes yields and bioactivities and in the biosynthesis of curcumin and bioactive components than SM treatments, but the biological pathway was not clear, still. This experiment suggests that curcumin or bioactive components affected by adding SM and OMWM could increase the yields and quality of turmeric.