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http://dx.doi.org/10.4062/biomolther.2013.048

Anticolitic Effect of the Rhizome Mixture of Anemarrhena asphodeloides and Coptidis chinensis (AC-mix) in Mice  

Jang, Se-Eun (Department of Life and Nanopharmaceutical Sciences and Department of Pharmaceutical Science)
Jeong, Jin-Ju (Department of Life and Nanopharmaceutical Sciences and Department of Pharmaceutical Science)
Hyam, Supriya R. (Department of Life and Nanopharmaceutical Sciences and Department of Pharmaceutical Science)
Han, Myung Joo (Department of Food and Nutrition, Kyung Hee University)
Kim, Dong-Hyun (Department of Life and Nanopharmaceutical Sciences and Department of Pharmaceutical Science)
Publication Information
Biomolecules & Therapeutics / v.21, no.5, 2013 , pp. 398-404 More about this Journal
Abstract
During a screening program to search the anticolitic herbal medicines, 80% ethanol extract of the rhizome of Anemarrhena asphodeloides (AA) was found to potently inhibit the expression of proinflammatory cytokines TNF-${\alpha}$ and IL-1${\beta}$, as well as the activation of NF-${\kappa}B$ in LPS-stimulated colonic macrophages, followed by that of the rhizome of C. chinensis (CC). AA also potently inhibited TNBS-induced colitic markers, shortening of the colon and increase of macroscopic score, myeloperoxidase activity, TNF-${\alpha}$, IL-1${\beta}$, and IL-6, in mice. The synergistic effect of CC against the anticolitic effect of AA was investigated. CC synergistically inhibited the anticolitic effect of AA. AC-mix (AA+CC, 1:1) potently inhibited them. AC-mix also inhibited the activation of NF-${\kappa}B$, as well as the expression of TNF-${\alpha}$, IL-1${\beta}$, IL-6, iNOS and COX-2. The effects of AC-mix against oxazolone-induced colitis were investigated in mice. AC-mix also potently inhibited oxazolone-induced inflammatory markers, colon shortening, macroscopic score, myeloperoxidase activity, NF-${\kappa}B$ activation and proinflammatory cytokines. Overall, the anti-colitic effect of AC-mix was superior to that of mesalazine. Based on these findings, AC-mix may improve colitis by inhibiting NF-${\kappa}B$ activation.
Keywords
Anemarrhena asphodeloides; Coptidis chinensis; AC-mix; Colitis;
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