• Title/Summary/Keyword: Rhizome

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Investigation of Emergence Conditions and Plug Seedling Periods in Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. (지황의 출아조건 및 플러그 육묘기간 구명 연구)

  • Lee, Sang Hoon;Koo, Sung Cheol;Hur, Mok;Lee, Woo Moon;Park, Min Su;Han, Jong Won
    • Korean Journal of Medicinal Crop Science
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    • v.27 no.4
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    • pp.271-277
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    • 2019
  • Background: Rehmannia glutinosa (Gaertn.) Libosch. ex Steud. has long been used as a traditional medicinal plant in Korea. This study was carried out to investigate the emergence conditions during the seedling periods in R. glutinosa. Methods and Results: The rhizomes of R. glutinosa variety (Jihwang 1) were harvested in the 22, March, 2018. The rhizomes were sown on in 50-cell plug trays. The emergence rates of seedlings at $15^{\circ}C$, $20^{\circ}C$, $25-40^{\circ}C$, and $45^{\circ}C$ treatment seedling were 1.3%, 96%, 100% and 0%, respectively. Rhizome rot was occurred at the temperature of $15^{\circ}C$ and $45^{\circ}C$. The emergence rates of seedlings in high moisture (HM), moderate moisture (MM) and low moisture (LM) treatments at $35^{\circ}C$ were 99.3%, 100%, and 0%, respectively. Drought damage was recorded in plant with the LM treatment. Seedling quality surveys were carried out at 10-days intervals from 10 to 60 days after sowing (DAS). Leaf length and leaf width were increasing until 50 DAS and the number of leaves was increasing until 60 DAS. Root length was increasing until 40 DAS, and then, flowering occurred from 30 to 60 DAS. Lastly, at 40 DAS, leaf aging and root enlargement was observed. Conclusions: We concluded that the emergence of seedlings was possible in the range of 20 to $40^{\circ}C$. Considering drying and rotting damage, we concluded that the moderate level of moisture is most appropriate for seedling emergence. In addition, we concluded that optimal seedling periods are between 30 and 40 DAS.

Establishment of efficient Alstromeria callus induction system using node culture and various hormones (마디배양과 다양한 호르몬을 이용한 효율적인 알스트로메리아 캘러스 유도 시스템 체계 확립)

  • Yang, Hwan Rae;Lee, Sang Hee;Kim, Jong Bo
    • The Journal of the Convergence on Culture Technology
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    • v.5 no.1
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    • pp.413-416
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    • 2019
  • Alstroemeria (Alstroemeriaceae) is one of the most important cut flowers in international market. Especially, characteristics like long vase-life, various colors, tolerance to low temperature and a low energy requirement during cultivation have stimulated this success. Because of its characteristics such as low multiplication rates, time-consuming process and high risk of carrying viral disease, in vitro propagation techniques based on rhizome meristems culture have been developing nowadays. The callus induction has various cultivation sites compared with the direct plant generation method, and if the callus is maintained well, the plant differentiation can be performed simultaneously while maintaining the callus, so that it can be used for mass proliferation. In this study, we tested various hormones and cultivars for efficient callus induction. As a result of culturing between the nodes and the internodes, the callus began to be formed after 8 weeks, and the calli incidence in the nodes was higher than that between the internodes. Also, in the comparison of 2,4-D and picloram, the callus incidence rate was up to 2 times higher in the medium treated with 2,4-D. Using these results, it is thought that it will help establish the system of mass propagation system of Alstroemeria and cultivate new varieties.

Effects of Heavy Metal Concentrations on Seed Germination and Rhizome Development of Campanula takesimana Nakai (중금속 농도가 섬초롱꽃 종자 발아 및 유근 발달에 미치는 영향)

  • Kim, Tae Hyun;Kim, Do Hyun;Im, Hyeon Jeong;Choi, Kyu Seung;Kim, Sang Geun;Song, Chi Hyeon;Oh, Beom Seok;Kim, Yang Su;Song, Ki Seon;Won, Chang O
    • Proceedings of the Plant Resources Society of Korea Conference
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    • 2019.10a
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    • pp.39-39
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    • 2019
  • 섬초롱꽃은 울릉군에 한정적으로 분포하는 여러해살이풀이다. 산림청에서는 약관심종(LC) 등급의 희귀식물이자 특산식물로 분류하고 있으며, 꽃의 관상가치가 특히 높아 화단용 소재, 절화용, 화분재배 등으로 흔히 이용되고 있다. 따라서 본 연구는 섬초롱꽃의 대량증식 시, 중금속이 미칠 수 있는 영향에 대해 파악하고자 수행하였다. 실험에 사용한 중금속은 카드뮴(Cd), 철(Fe) 두 종류이며, 두 종류 모두 1% 한천(agar)배지에 10, 25, 50, 100, 200 mg/L 농도로 녹여 사용하였다. 발아율 및 유근 생장을 위해 종자 및 유묘를 치상 후, $25^{\circ}C$ 항온 생장상에서 발아율 및 유근 발달을 관찰하였다. 발아율 측정결과, 아무처리도하지 않은 1% 한천(agar) 배지에서 $62.7{\pm}7.4%$로 가장 높았고 중금속 농도가 높아질수록 감소하였다. 카드뮴(Cd) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 $17.3{\pm}1.3$, $2.7{\pm}1.3$, $1.3{\pm}1.3$, 0, 0%로 나타났다. 한편, 철(Fe) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 $46.7{\pm}4.8$, $24.0{\pm}2.3$, $36.0{\pm}6.9$, $20.0{\pm}2.3$, 0%로 나타났다. 유근의 평균 생장 길이(mm)는 아무처리도 하지 않은 1% 한천(agar) 배지에서 19.6 mm로 가장 높았고, 중금속 농도가 높아질수록 낮게 나타났다. 카드뮴(Cd) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 4.6 mm, 1.9 mm, 0.7 mm, 0.9 mm, 0 mm 생장한 것으로 나타났다. 한편, 철(Fe) 처리구에서는 10, 25, 50, 100, 200 mg/L 농도에서 각각 15.9 mm, 8.6 mm, 8.1 mm, 0.9 mm, 0 mm 생장한 것으로 나타났다. 실험결과를 종합해 볼 때, 섬초롱꽃 종자 발아 및 유근 생장의 억제는 카드뮴(Cd) 처리구에서 높게 나타났고, 철(Fe) 처리구에서는 비교적 영향을 덜 미치는 것으로 나타났다. 특히 카드뮴은 10 mg/L 농도만 처리하더라도 약 3.6~4.2배의 발아율 생장율 감소가 나타나, 섬초롱꽃 대량증식 시, 치명적일 것으로 판단된다. 본 실험의 결과는 섬초롱꽃 종자를 이용한 대량증식 및 유묘재배에 있어, 토양을 선택하는데 중요한 기초자료로서 활용될 것으로 기대된다.

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A Study on the Expressions of Rhizomatic Escape by Deleuze and Guattari - Song Hayoung With a focus on paintings and objet works - (들뢰즈와 가타리의 리좀적 탈주 표현 연구 -송하영 회화·오브제작품을 중심으로-)

  • Song, Hayoung
    • The Journal of the Convergence on Culture Technology
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    • v.7 no.4
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    • pp.325-330
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    • 2021
  • This study set out to investigate the forms, attributes, and escape methods of post-subjects projected on the investigator's works in connection with rhizomatic thinking proposed as a way of social transformation by Deleuze and Guattari and examine their social connotations. Post-subjects projected on the investigator's works are not completed wholes of some sort, but like materials whose constant premise is change and creation. In the investigator's works, post-subjects have conscious and unconscious desire. It is the desire of creation with positive attributes including Deleuze's and Guattari's pursuit of changes in a contradicting society. When desire is deployed in post-subjects, they will carry out an escape. This way of escape is rhizomatic proposed by Deleuze and Guattari. It deconstructs contradicting things and repeats connection, contact, and severance with the outside world, building a new order. Rhizomatic post-subjects appearing in the investigator's works depict the escape process and method in abstract ways through the variable installation of objets combined with a color field of repeating brushes. In this work, the goal of post-subjects is to make a safe landing in a space where beings are recognized for their values and free and creative lives. These post-subjects are nomads creating a new landscape continuously, wandering around vast plains, and also artists and literary figures resisting a contradicting society. That is, they are connected to the concept of a war machine proposed by Deleuze and Guattari as a concept of social transformation and to the concept of Nietzsche's Agon to devise and create new values and politics based on street passion. They seek after a space where they can co-exist with otherness recognized rather than the complete deconstruction of the old order.

Exploration of Beneficial Herbal Medicines to Attenuate Particulate Matter-induced Cellular Injury in Human Corneal Epithelial Cells (인간 각막상피세포에서 미세먼지로 인한 세포 손상을 완화할 수 있는 유익한 한약재의 탐색)

  • Kim, Da Hye;Kim, Min Yeong;Hwangbo, Hyun;Ji, Seon Yeong;Park, Seh-Kwang;Park, Sung-Ho;Kim, Mi-Young;Choi, Yung Hyun
    • Journal of Life Science
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    • v.32 no.8
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    • pp.647-658
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    • 2022
  • Particulate matter (PM) is known to be involved in the onset and progression of various diseases by promoting oxidative and inflammatory reactions as air pollutants containing various small particles that are harmful. In this study, the protective efficacy of herbal medicines was evaluated in human corneal epithelial cells (hCECs) to select natural products that can protect the eye, the primary organ directly exposed to external pollutants from PM. As a result, five candid ate herbal medicines [Cheonmundong, Asparagus Rhizome; Seokchangpo, Aciru Gramineri Rhizoma; Hwangryeon, Coptidis Rhizoma; Gamgug, Chrysanthemi Indici Flos; and Geumjanhwa (Marigold flower petals)] which showed inhibitory efficacy on PM2.5-induced cytotoxicity, were selected from among 12 candidate herbal medicines. To evaluate the antioxidant activity of these candidate substances, the reactive oxygen species (ROS) scavenging ability was investigated, and it was found that the extracts of Seokchangpo, Cheonmundong and Hwangryeon showed a significant inhibitory effect on PM2.5-induced ROS production, which was correlated with the preservation of mitochondrial activity. In addition, it was confirmed that they could block DNA damage caused by PM2.5 through analysis of 8-hydroxy-2'-deoxyguanosine generation and phosphorylated-H2A histone family member X (γ- H2AX) expression. Furthermore, the increase in inflammasome activity and inflammatory response in PM2.5-treated hCECs was also canceled in the presence of these extracts. Although additional studies are needed, the results of this study will be used as primary data to find novel natural compounds that protect hCECs from PM.

Safety of Oriental Medical Therapy: Drug Fever of Herbal Medicine and Procedure-related Fever (한방치료의 안전성 : 한약에 의한 약열과 시술관련 발열)

  • Moon, Soo-youn;Lim, Kyoung Ree;Son, Jun Seong
    • The Journal of Internal Korean Medicine
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    • v.42 no.6
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    • pp.1237-1244
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    • 2021
  • Objectives: Drug fever and procedure-related fever are the causes of nosocomial fever. Oriental medicine has been practiced in Asia and is now being practiced as an alternative medicine in western countries. No data are available on the incidence of drug fever and procedure-related fever in oriental medical hospitals (OMHs). The aim of this study was to identify the incidence of drug fever related to oriental herbal medicine and oriental medical procedure-related fever. Methods: This was a retrospective study at one OMH of a university medical institute in Seoul, Korea, conducted from June 2006 to June 2013. Results: Overall, 95 episodes of drug fever occurred among 10880 patients treated with herbal medicine (0.89%). Peak body temperature was 38.37±0.58 ℃, and the fever lasted for 1.0 day (range 1.0-17.0 days). Eosinophilia was found in 15 patients (15.79%) and 8 patients developed toxic hepatitis (8.42%). Five patients had a drug-related skin rash (5.26%). The most common ingredients of the herbal medicines associated with drug fever were licorice, Angelica gigas root, and white Atractylodes rhizome. In total, 16 episodes of procedure-related fever (0.20%) occurred in 8125 patients treated with oriental medical procedures. The peak body temperature was 38.26±0.51 ℃, and the fever lasted for 1.0 day (range 1.0-3.0 days). Among various oriental medical procedures, moxibustion was the most common procedure related to fever, followed by acupuncture. Conclusions: The incidence of drug fever and procedure-related fever in oriental medicine is not high compared with the incidence in western medicine.

Inhibition Effects on Oxidative DNA Damage and Anti-inflammatory Effects of Nelumbinis Flos (연꽃의 산화적 DNA 손상 억제 활성 및 항염증 효과)

  • Jeong, Hyung Jin;Park, Yeon Gyeong;Jang, Tae Won;Kim, Do Wan;Jeong, Jin Boo;Park, Jae Ho
    • The Korea Journal of Herbology
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    • v.32 no.3
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    • pp.45-53
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    • 2017
  • Objective : Nelumbo nucifera, its rhizome and semen have been used as a traditional medicine which was studied on antioxidant, hepatoprotective effect, anti-obesity and the others. However, Nelumbinis Flos have not studied. We investigated protective effects on oxidative DNA damage and anti-inflammatory effects of Nelumbinis Flos. Methods : The antioxidant activity was conducted by 1,1-diphenyl-2-picryl hydrazyl (DPPH) radical, 2, 2'-Azino-bis (3-ethylbenzothiazoline-6 sulfonic acid) diammonium salt (ABTS) radical scavenging assay and reducing power assay. Total phenolic content was analyzed. Also, phenolic compounds were detected by HPLC/UV. The inhibitory effect on oxidative DNA damage was determined using ${\Phi}X-174$ RF I plasmid DNA cleavage assay. The anti-inflammatory effect of Nelumbinis Flos was measured by the amount of nitric-oxide (NO) produced and protein levels of iNOS, and COX-2 in LPS induced RAW 264.7 cells. Results : The results of DPPH and ABTS radical scavenging activity at $200{\mu}g/m{\ell}$ of extraction were $97.02{\pm}0.88%$ and $96.42{\pm}0.25%$. Reducing power (fold of L-ascorbic acid as control) was $100.14{\pm}0.31$ at $200{\mu}g/m{\ell}$. Total phenol content was $8.70{\pm}0.02mg/g$. Chlorogenic acid, catechin and epicatechin were found by HPLC. Nelumbinis Flos has inhibitory effect in dose-manner against oxidative DNA damage. In addition, it showed the anti-inflammatory effect by suppression of NO production as well as protein levels of iNOS, and COX-2. Conclusion : This study suggested that Nelumbinis Flos showed potential antioxidant and suppression activities of various factors were related in NO produced. Therefore, Nelumbinis Flos as natural plant resources that may help reduce inflammation and alleviate DNA damage.

A Study on Becoming Science Teachers Seen in Pre-service Elementary Teachers' Inquiry into the Features and Roles of Tree Stems: Focusing on the Degree of Attaining Deterritorialization and the Types of Rhizomatic Thoughts (초등 예비교사들의 '줄기의 생김새와 하는 일' 탐구에서 나타난 과학교사 되기 탐색 - 탈영토화 도달 정도와 리좀적 사유 유형을 중심으로 -)

  • Kim, Dong-Ryeul
    • Journal of Korean Elementary Science Education
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    • v.41 no.1
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    • pp.1-13
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    • 2022
  • This study aimed to explore 'Becoming Science Teachers' seen in pre-service elementary teachers' inquiry into the features and roles of tree stems, based on Deleuze's Theory of Becoming. As research subjects, this study selected 30 pre-service elementary teachers and made them carry out textbook activities and advanced activities for about a month. Based on the results of these activities, they kept reflective inquiry diaries, and this study analyzed them and found out how far they attained according to Deleuze's mechanism of generating differences. Interestingly, it was found out that most of them attained the phase of escape and even the phase of deterritorialization and showed the highest frequency of establishing various know-hows about inquiries needed to teach students in the future. However, it was also analyzed that over 60% of the diaries still remained in phases prior to the phase of deterritorialization through escape. When analyzing rhizomatic thought that helped attain deterritorialization through escape, this study found out that all the 6 types were seen and functioned to attain deterritorialization. Therefore, it is necessary to provide challenging inquiry environments that pre-service elementary teachers have never experienced before so that they can go through a phased process for overcoming striated space in inquiry and attain reterritorialization.

Analysis of Chemical Components for Aerial and Underground Parts of Wild Ginseng and Evaluation of Skin Anti-aging Efficacy (야생 산삼 지상부 및 지하부의 화학성분 분석과 피부 항노화 효능 평가)

  • Seok-Seon Roh;Gwang Jin Lee;Byunghyun Kim;Bo Kyoung Hwang;Hyojin Kim;Yun Hee Chang;Jae-kun Yoou;Young-Sung Ju
    • The Korea Journal of Herbology
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    • v.38 no.5
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    • pp.85-95
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    • 2023
  • Objectives : This study was intended to reveal the chemical profiles of aerial(leaf, stem) and underground(rhizome, radix) parts of wild ginseng, and to investigate their anti-aging effects on human skin cells. Methods : Wild ginseng, estimated for over 20 years, was divided into the aerial and underground parts. Total phenolic contents of each extracts were measured using a Folin-ciocalteu method. The contents of 18 amino acids, 8 minerals and 27 ginsenosides were determined by GC-FID, ICP-MS and LC-MS, respectively. The anti-aging effects, including the radical scavenging activity, the activation of mitochondrial function on human fibroblasts, and the proliferation activity on human keratinocyte progenitor cells, for the whole plant and underground part of wild ginseng were evaluated. Results : The total phenolic acids, amino acids, and minerals in the aerial part were more than twice as high as in the underground part. Compared to the cultivated ginseng root, there were various types of ginsenosides in both parts of wild ginseng, and the total amount was more than twice as high. In particular, the aerial part significantly contained ginsenoside F1, F2, C-Mc1, and C-O, and the distinctive patterns that distinguish each parts of wild ginseng from the cultivated ginseng root were derived. The whole plant and underground part of wild ginseng exhibited significant antioxidant effect(14.3-45.6%), activation of mitochondrial membrane potential(105.5-120.1%), and cell proliferation(112.1-125.4%). Conclusions : The entire plant and underground part of wild ginseng are high value-added plants and have beneficial effects on skin anti-aging properties through its abundant metabolites.

A Study on External.Internal Morphology and Pattern Analysis of Atractylodes Rhizomes (출류(朮類) 한약재의 외.내부형태와 이화학패턴 연구)

  • Kim, Jung-Hoon;Lee, Guem-San;Choil, Goya;Hwang, Sung-Yeoun;Kim, Hong-Jun;Jeong, Seung-Il;Ju, Young-Sung
    • The Korea Journal of Herbology
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    • v.24 no.2
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    • pp.77-85
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    • 2009
  • Objectives : To determine the discriminative criteria for Atractylodes rhizomes, the experiment of externalinternal characteristics and physicochemical pattern analysis were performed. Methods : External characteristics was observed using stereoscope. The sectioned materials which were covered with parffin were stained by Ju's method. Physicochemical patterns were analyzed using HPLC/DAD. Results : 1. External shape of original plant : Atractylodes maaocephala and A. japonica had relatively long petioles and 3-5 parted leaves. A. macrocephala had big purple flowers whereas A. japonica had relatively small white flowers and pinnate bracts. A. lancea had sessile leaves and white flowers, and the end parts of degenerated stamens were bent. 2. External shape of herbal medicine: A. macrocephala which was fist-shaped rhizome had pa-pillate processes and the cross section was light gray and sulcate. A. japonica and A. lancea were connected-beady or tubercular rhizomes, and the cross sections were both yellow-colored white. However, the cross section of A. japonica was fibrous, the width of cortex was narro-wer than that of stele, and radial shape in cortex was rare, whereas the width of cortex in A. lancea was similar to that of stele in size, and radial shape in cortex was obvious. 3. Internal shape of herbal medicine: A. macrocephala and A. lancea did not have lignified fascicles in cortex. However, the vascular bundles and vessels of A. macrocephala were wedge shaped and radial arrangement, and vascular bundles were densely populated in stele whereas those of A. lancea were repeatedly arranged and thinly extended to cortex. A. japonica had lignified fascicles in cortex and the width of vascular bundles was conspicuously thick with narrow intervals. 4. Physicochemical pattern analysis : A. macrocephala and A. lancea contained atractylenolide I and atractylenolide Ill whereas A. japonica contained atractylenolide I, atractylenolide Ill. diacetyl-atractylodiol, compound-4, compound-5. The three species of Atractylodes rhizomes showed different chromatogram patterns. Conclusions : The results could be used as discriminative criteria for Atractylodes rhizomes and as fundamental materials to researches of further pattern analysis and biological reaction.