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Characteristics of Acid Pre-treated Red Ginseng and Its Decoction (산처리 홍삼과 추출물의 특성)

  • Kim, Mi-Hyun;Lee, Young-Chul;Choi, Sang-Yoon;Cho, Chang-Won;Rho, Jeong-Hae
    • Journal of Ginseng Research
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    • v.33 no.4
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    • pp.343-348
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    • 2009
  • This study was conducted to produce a new red ginseng by steaming ginseng using a new pre-treatment method, so as to develop ginseng products with improved flavor and thereby expand ginseng's consumer base. The color parameters (Hunter value), free sugar contents, and ginsenoside contents of the powder from the dried red ginseng, and the sensory test of the semi-dried red ginseng and the decoction from the dried red ginseng, were measured to investigate the effect of acid (ascorbic acid or citric acid) impregnation pre-treatment on red ginseng. The powder from the acid-pretreated red ginseng became lighter and more yellow than the red ginseng that was not pre-treated, but the redness (avalue) of the powder from the acid-pre-treated red ginseng increased. The ginsenoside contents of $Rg_2+Rh_1$ and $Rg_3$ increased with the acid treatment. There was a significant difference in the color and sweetness of the semi-dried acidtreated and non-treated red ginsengs in the sensory test. As the results of the sensory test were expressed in the hedonic scale, however, there were significant differences in the degrees of bitterness, astringency, sourness, odor, and color of the red ginseng decollation. Especially, the acid-treated red ginseng extract tasted less bitter, which shows the potential of new red ginseng products with improved ginseng flavor.

Establishment of Optimal Fermentation Conditions for Steam-dried Ginseng Berry via Friendly Bacteria and Its Antioxidant Activities (생체친화성 균주에 의한 인삼열매증포 추출물의 최적발효조건 및 항산화활성)

  • Kim, Seung Tae;Kim, Hee Jung;Jang, Su Kil;Lee, Do Ik;Joo, Seong Soo
    • Korean Journal of Food Science and Technology
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    • v.45 no.1
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    • pp.77-83
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    • 2013
  • In this study, we observed optimal conditions and suitable bacteria for the fermentation of steam-dried ginseng berry extracts (SGB) and determined antioxidant effects of the fermented extracts. Five bacteria (Lactobacillus fermentarum, L. plantarum, L. brevis, L. casei, Bacillus subtillis) were examined on their growth activities and viabilities in various culture temperatures ($25-35^{\circ}C$) and concentrations (25-100%). L. plantarum was considered to be the most suitable bacteria for the fermentation in both growth activity and viability. Moreover, the extracts fermented with L. plantarum showed more potent antioxidant efficacy in both 1,1-diphenyl-2-picrylhydrazyl radical and hydroxyl radical scavenging assay. High performance liquid chromatography analysis revealed that fermentation with L. plantarum changed the contents and components of ginsenosides. In conclusion, these data suggest that L. plantarum efficiently ferment SGB and the fermented extracts may have therapeutical values against oxidative stress and be a good candidate in adjuvant therapy where ginsenoside would be the main composition.

Isolation and Identification of Antagonistic Bacterium Active against Sclerotinia sclerotioum Causing Sclerotinia Rot on Crisphead Lettuce (결구상추 균핵병균(Sclerotinia sclerotioum)에 대한 길항세균의 분리 및 동정)

  • Kim, Han-Woo;Lee, Kwang-Youll;Baek, Jung-Woo;Kim, Hyun-Ju;Park, Jong-Young;Lee, Jin-Woo;Jung, Soon-Je;Moon, Byung-Ju
    • Research in Plant Disease
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    • v.10 no.4
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    • pp.331-336
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    • 2004
  • The fungus genus Sclerotinia contains a number of important plant pathogens. Vegetable growers in our country are probably most familiar with Sclerotinia sclerotiorum, the causes of sclerotinia rot on crisphead lettuce. S. sclerotiorum has a wide host range which can include lettuce as well as crops such as broccoli, cabbage, carrots, celery, beans, peppers, potatoes, stocks, and tomato. Some fungicides, including benomyl, are effective in some crops, but not all. So, we isolated a antagonistic bacteria that are active on sclerotinia rot caused by S. sclerotiorum and that can be used to control it. About 702 strains had been isolated from soil around plant roots in the field. Ten strains showed strong antifungal activity against S. sclerotiorum. In pot test for antagonistic activity, A-7 strain showed high control value against the pathogen when compared with others. The strain was, therefore, selected as a biocontrol candidate against sclerotinia rot and its biochemical properties and 16S rDNA sequence was analyzed. The A-7 strain was highly related to Bacillus subtilis and B. amyloliquefaciens. To confirm precise identification, we had performed gyr A gene sequences analysis. Its sequence had 96% similarity with B. amyloliquefaciens. Consequently, the isolate was identified as B. amyloliquefaciens A-7.

Test of Insecticidal Efficacy of Some Commercial Natural Products against Trialeurodes vaporariorum(Homoptera : Aleyrodidae), Bemisia tabaci(Homoptera : Aleyrodidae), and Spodoptera litura(Lepidoptera : Noctuidae)

  • Jeong, Hyung-Uk;Kim, Man-Il;Chang, Sung-Kwon;Oh, Hyung-Keun;Kim, Ik-Soo
    • International Journal of Industrial Entomology and Biomaterials
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    • v.18 no.2
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    • pp.105-112
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    • 2009
  • The greenhouse whitefly, Trialeurodes vaporariorum (Westwood) and the sweet potato whitefly, Bemisia tabaci (Gennadius) are serious insect pests that have a wide host range including cucumber, tomato, and pepper. In this study, we tested larvicidal efficacy of several on-the-market environment-friendly agricultural materials (EFAM) to select the effective products after the target pests were stabilized in indoor rearing condition. The developmental periods of two whiteflies are as follows: in the case of T. vaporariorum, egg duration is 9.6 days and nymph is 18.9 days, and in the case of B. tabaci, egg duration is 7.4 days and nymph is 15.2 days under $25^{\circ}C$ with relative humidity (RH) of $60{\pm}5%$ and a photoperiod of 16 L:8D. The total period of T. vaporariorum was 5 days longer than B. tabaci. Among 22 EFAMs six products showed more than 60% of insecticide efficacy against T. vaporariorum BTV B, BTV D, BTV G, BTV L, BTV M, and BTV S. On the other hand, seven EFAM products showed over 60% of insecticide efficacy against B. tabaci BTV D, BTV G, BTV K, BTV L, BTV M, BTV N, and BTV U. In the case of Spodptera litura previously, 16 EFAMs were tested against $2^{nd}$ instar S. litura, and six EFAMs were found to have more than 90% efficacy. Test of these six EFAMs against entire larval stages were performed in this study. Although some of these products showed still more than 90% of insecticidal efficacy against up to $3^{rd}$ instar larvae, the efficacy of these EFAMs sharply decreased as ages increase, resulting in less than 60% of efficacy of the products at most. This result indicates the difficulty to control S. litura with the on-the-market EFAMs alone under economic injury level. Collectively, it is required to find more EFAMs, and find alternative method, and combined way of controlling to control those insect pests tested in this study.

A Study on the Crease Recovery Behavior of Core-spun Yarn Woven Fabrics (코어방적사직물의 구김회복거동에 관한 연구)

  • Kwon Ok-Kyung;Sung Su-Kwang;Kim Hyo-Dae
    • Journal of the Korean Society of Clothing and Textiles
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    • v.13 no.3 s.31
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    • pp.259-267
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    • 1989
  • In this paper, the fabric specimen undergoes repeated laundering under given condition. After this cyclic laundering was applied, the crease recoveries of the specimen were measured using shirley crease revovery tester in order to evaluate the effect of factors at given condition during crease deformation. 5 samples of grey plain cloth were desized, alkali-scoured, bleached, whased with water, and air-dried. All tests were made on samples preconditioned to $65\%\;RH\;and\;20^{\circ}C$. The experimental results were analysed statistically to relate crease recoveries and the properties of smaples, recovery periods (time) of crease. Furthermore, the crease recoveries of core-spun yarn woven fabrics were discussed in comparison with those values for $100\%$ combed cotton yarn woven fabric and $65\%$ polyester $35\%$ carded cotton blended yarn woven fabric. The results obtained are as follows; 1. Regardless of materials, remarkable decrease are observed in crease recoveries about 1-5 cycles of the repeated laundering, but slack decrease are observed in crease recoveries after 5 cycle of the re-peated laundering. 2. Crease recoveries ($\alpha$) of core-spun yarn woven fabrics are relate to recovery periods (t) of crease as follows; log$\alpha$=0.01415 log t+2.1168 ($r^2=0.94$) 3. Core-spun yarn woven fabrics were superior to $100\%$ combed cotton yarn woven fabrics and $65\%$ polyester $35\%$ carded cotton blended yarn woven fabric in crease recoveries. 4. Crease recoveries ($\alpha$) of core-spun yarn woven fabrics are relate to cover factor (CF), thickness (T) at pressure 0.5 $gf/cm^2$, weight (W) as follows; log$\alpha$=-0.3482 log CF-0.4924 log T-0.4727 W+2.4243 ($r^2=0.88$) 5. Crease recoveries ($\alpha$) of core-spun yarn woven fabrics are relate to 2HB/B, 2HB/W, $\sqrt[3]{B/W}$, WC/T which are concerning to formation of weared clothes and bending Iran formation behavior as follows: log $\alpha$=0.0091 2HB/B+0.4667 2HB/W+0.0185 $\sqrt[3]{B/W}$+0.0114 WC/T+1.8433 ($r^2=0.86$)

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Formation of the Fluorite Structure in the $\textrm{Y}_{0.8}\textrm{Ta}_{0.2}\textrm{O}_{1.7}$-MO(M=Ba, Sr, Ca and Mg) System ($\textrm{Y}_{0.8}\textrm{Ta}_{0.2}\textrm{O}_{1.7}$-MO(M=Ba, Sr, Ca 및 Mg)계에 있어서 형석구조의 생성)

  • Kim, Shin;Choi, Soon-Mok;Lee, Hong-Lim
    • Korean Journal of Materials Research
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    • v.7 no.1
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    • pp.57-61
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    • 1997
  • Formation of fluorite structure and other related crystal structures in the $Y_{0.8}Ta_{0.2}O_{1.7}$-MO(M=Ba, Sr, Ca and Mg) system has been studied $Ba_2YTaO_6,\;Sr_2YTaO_6$ of cubic perovskite type ordered structure anti $Y_2O_3$ of cubic structure were produced besides the defect fluorite structure when 4 moIob of BaO or SrO was added to $Y_{0.8}Ta_{0.2}O_{1.7}$ When CaO more than 8 nlol"/o was added to $Y_{0.8}Ta_{0.2}O_{1.7}$, monoclinic: $Ca_2YTaO$, and cubic $Y_2O_3$ were pri~tlucecl ;IS this sec:onci phases hesides the main fluorite truc,ture. Smglc phase of fluorite structure \vas 1)roduc:ciI when MgO was added up to 12 mol%, however, MgO appeared as the second phase besides the main fluorire structure when MgO was added more than lti moI0'. Consquently, it is considered rh;it the formation of tluorite structure is related with the formation of the cubic perovskite type ordered structure of $A_2(B'B")O_6$ as well as the cation radii of the additives.additives.

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Prognostic Value of Day 3 Inhibin-B on Assisted Reproductive Technology Outcome (보조 생식술 결과에 있어서 기저혈중 Inhibin-B의 예후인자로서의 유용성)

  • Bai, Sang-Wook;Kim, Jin-Young;Lee, Kyung-Sool;Won, Jong-Gun;Lee, Yong-Joo;Yi, Ji-Won;Chang, Kyung-Hwan;Lee, Byung-Seok;Park, Ki-Hyun;Cho, Dong-Jae;Song, Chan-Ho
    • Clinical and Experimental Reproductive Medicine
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    • v.24 no.2
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    • pp.217-223
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    • 1997
  • This study was performed to determine if women with day 3 serum inhibin-B concentrations <45pg/ml (conversion factor to SI unit, 1.00) demonstrate a poor response to ovulation induction and assisted reproductive technology outcome to women with inhibin-B${\ge}45pg$/ml, independant of day 3 FSH, E2 and patient age. From Jan 1996 to Dec 1996, 16 volunteers patients who underwent 25 IVF cycles with luteal phase GnRH agonist suppression and HMG stimulation were allocated to the study group. We evaluated day 3 serum inhibin-B, FSH, E2, peak E2, cancellation rate per initiated cycle (%) and clinical pregnancy rate per initiated cycle (%) according to the above two groups and independent of patient age, day 3 FSH, day 3 E2 and all of above combined. Women with day 3 serum inhibin-B${\ge}45pg$/ml demonstrated higher average day 3 inhibin-B level, clinical pregnancy rate per initiated cycle ($20.3{\pm}2.5$ pg/ml vs $80.9{\pm}5.0$ pg/ml, p<0.05; 24.8% vs 8.5%, p<0.05) and lower day 3 FSH level, cancellation rate per initiated cycle ($6.9{\pm}0.3$ mIU/ml vs $8.5{\pm}0.5$ mIU/ml, p<0.05; 1.5% vs 9.0%, p<0.05). Women with day 3 serum inhibin${\ge}45pg$/ml and age<40 year demonstrated higher pregnancy rate per initiated cycle (28.2% vs 7.4%, p<0.05) and lows. day 3 FSH level, cancellation rate per initiated cycle ($6.9{\pm}0.5$ mIU/ml vs $8.2{\pm}0.7$ mIU/ml, p<0.05; 1.0% vs 9.0%, p<0.05). Women with day 3 serum inhibin${\ge}45pg$/ml and day 3 FSH<15mIU/ml demonstrated higher pregnancy rate per initiated cycle (33.5% vs 9.5%, p<0.05) and lower day 3 FSH level, cancellation rate per initiated cycle ($7.7{\pm}0.2$ mIU/ml vs $8.5{\pm}0.5$ mIU/ml, p<0.05; 1.5% vs 10.0%, p<0.05). Women with day 3 serum inhibin${\ge}45pg$/ml and day 3 E2<50pg/ml demonstrated higher pregnancy rate per initiated cycle (30.0% vs 9.5%, p<0.05) and lower cancellation rate per initiated cycle (1.5% vs 9.5%, p<0.05). Women with day 3 serum inhibin${\ge}45pg$/ml, age<40 year, day 3 FSH<15mIU/ml and day 3 E2<50pg/ml demonstrated higher pregnancy rate per initiated cycle (30.0% vs 10.8%, p<0.05) and lower day 3 FSH level, cancellation rate per initiated cycle ($6.8{\pm}0.6$ mIU/ml vs $8.4{\pm}0.9$ mIU/ml, p<0.05; 1.5% vs 7.8%, p<0.05). Therefore women with low day 3 serum inhibin-B concentrations demonstrate a poorer response to ovulation induction and are less likely to conceive a clinical pregnancy though ART relative to women with high day 3 inhibin-B and day 3 serum inhibin-B, in addition to a day 3 FSH, E2 and patient age, appears helpful in prediction in IVF-ET outcome.

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Chromosomal Localization and Mutation Detection of the Porcine APM1 Gene Encoding Adiponectin (Adiponectin을 암호화하는 돼지 APM1 유전자의 염색체상 위치파악과 돌연변이 탐색)

  • Park, E.W.;Kim, J.H.;Seo, B.Y.;Jung, K.C.;Yu, S.L.;Cho, I.C.;Lee, J.G.;Oh, S.J.;Jeon, J.T.;Lee, J.H.
    • Journal of Animal Science and Technology
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    • v.46 no.4
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    • pp.537-546
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    • 2004
  • Adiponectin is adipocyte complement-related protein which is highly specialized to play important roles in metabolic and honnonal processes. This protein, called GBP-28, AdipoQ, and Acrp30, is encoded by the adipose most abundant gene transcript 1 (APM1) which locates on human chromosome 3q27 and mouse chromosome 16. In order to determine chromosomal localization of the porcine APM1, we carried out PCR analysis using somatic cell hybrid panel as well as porcine whole genome radiation hybrid (RH) panel. The result showed that the porcine APM1 located on chromosome 13q41 or 13q46-49. These locations were further investigated with the two point analysis of RH panel, revealed the most significant linked marker (LOD score 20.29) being SIAT1 (8 cRs away), where the fat-related QTL located. From the SSCP analysis of APM1 using 8 pig breeds, two distinct SSCP types were detected from K~ native and Korean wild pigs. The determined sequences in Korean native and Korean wild pigs showed that two nucleotide positions (T672C and C705G) were substituted. The primary sequence of the porcine APM1 has 79 to 87% identity with those of human, mouse, and bovine APM1. The domain structures of the porcine APM1 such as signal sequence, hypervariable region, collagenous region. and globular domain are also similar to those of mammalian genes.

Effect of rbST Administrations at Artificial Insemination on Conception and Parturition Rates in Hanwoo (한우 인공수정시 rbST 투여가 수태 및 분만율에 미치는 영향)

  • Han M. H.;Choi S. H.;Choi Y. H.;Kim H. J.;Cho S. R.;Choi C.Y.;Ryu I. S.;Son D. S.;Yeon S. H.;Woo J. S.;Kweon U. G.;Yoon K. Y.;Chang B. S.
    • Journal of Embryo Transfer
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    • v.20 no.2
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    • pp.177-184
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    • 2005
  • This study was conducted to investigate the effects of recombinant bovine somatotropin (rbST) injection on conception and parturition rates in normal or repeat breeding Hanwoo. We treated 462 cows containing 79 repeat-breeding cows of multiparous and allocating 5 treatment groups. Treatment 1 (T1) was injection of 2ml saline (for pseudo treatment), T2 was one injection of rbST 250mg into the tailhead region at the estrus, T3 was twice injection of rbST 250mg both at the time of insemination and again 10 to 14 day later, T4 was once injection of rbST 500mg at insemination and T5 was twice injection of 500mg rbST both at the time of insemination and again 10 to 14day later respectively. In rbST treated groups, timed artificial inseminations (TAI) were performed fellowing estrus synchronization. 100 us GnRH was injected into the scapula region on Day 0, 25mg $PGF_2{\alpha}$ was injected on Day 7 for degeneration of corpus luteum (CL) and 100ug GnRH was injected for inducing the synchronization. The results are as fellows; When normal Hanwoo were inseminated once with rbST administration, the pregnancy rate of T2 $(67.5\pm18.48\%)$ were higher than control $(52.4\pm9.72\%)$, while the pregnancy rate of T4 $(63.3\pm5.77\%)$ were significantly higher (p.<0.05) than control $(39.3\pm12.89\%)$ in repeat breeder Hanwoo. The parturition rates of normal Hanwoo were no differences among the treatments but were significant different in repeat breeder Hanwoo (p<0.05). When the estrous was induced by Ovsynch and inseminated once with rbST administration, the pregnancy rates of T2 was $12.5\%$ higher than control in normal Hanwoo, T4 $(80.0\%)$ was highest among the treatments (p<0.05) in repeat breeder Hanwoo. When normal Hanwoo were inseminated once with rbST administration, the pregnant period was $282.7\~284.8$ days and the body weight was $25.1\~25.9kg$, there were no difference among the treatments. The ratio of sex was almost same without T4 (male vs. female=18:9). In repeat breeder Hanwoo, pregnant period was 280.4~289.3 day and body weight was $23.0\~26.6kg$, it had no difference among the treatments. The sex ratio were similar to normal Hanwoo except T4 (M : F=2 : 8). In conclusion, the pregnancy and parturition rate by once insemination could be improved by the administration of rbST 250mg in normal Hanwoo or 500mg in repeat breeder Hawoo.

New Synthesis of the Ternary Type Bi2WO6-GO-TiO2 Nanocomposites by the Hydrothermal Method for the Improvement of the Photo-catalytic Effect (개선된 광촉매 효과를 위한 수열법에 의한 삼원계 Bi2WO6-GO-TiO2 나노복합체의 쉬운 합성 방법)

  • Nguyen, Dinh Cung Tien;Cho, Kwang Youn;Oh, Won-Chun
    • Applied Chemistry for Engineering
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    • v.28 no.6
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    • pp.705-713
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    • 2017
  • A novel material, $Bi_2WO_6-GO-TiO_2$ composite, was successfully synthesized using a facile hydrothermal method. During the hydrothermal reaction, the loading of $Bi_2WO_6$ and $TiO_2$ nanoparticles onto graphene sheets was achieved. The obtained $Bi_2WO_{6-GO-TiO2}$ composite photo-catalysts were characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM), energy dispersive X-ray (EDX) analysis, transmission electron microscopy (TEM), Raman spectroscopy, ultraviolet-visible diffuse reflectance spectroscopy (UV-vis-DRS), and X-ray photoelectron spectroscopy (XPS). The $Bi_2WO_6$ nanoparticle showed an irregular dark-square block nanoplate shape, while $TiO_2$ nanoparticles covered the surface of the graphene sheets with a quantum dot size. The degradation of rhodamine B (RhB), methylene blue trihydrate (MB), and reactive black B (RBB) dyes in an aqueous solution with different initial amount of catalysts was observed by UV spectrophotometry after measuring the decrease in the concentration. As a result, the $Bi_2WO_6-GO-TiO_2$ composite showed good decolorization activity with MB solution under visible light. The $Bi_2WO_6-GO-TiO_2$ composite is expected to become a new potential material for decolorization activity. Photocatalytic reactions with different photocatalysts were explained by the Langmuir-Hinshelwood model and a band theory.