• The Journal of the Acoustical Society of Korea
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• v.36 no.1
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• pp.39-48
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• 2017

CDMA (Code Division Multiple Access) is promising medium access control schemes for underwater acoustic sensor networks because of its robustness against frequency-selective fading and high frequency-reuse efficiency. In this paper, we design diversity schemes of underwater CDMA transceiver for the forward and reverse links. User data are multiplexed by Walsh code and a pseudo random noise code acquisition process is added for phase error correction before decoding the user data at the receiver. Then, the diversity reception using equal gain combining and maximal ratio combining is performed in order to minimize performance degradation caused by rich multipath fading of underwater acoustic channel. We evaluated the performance of diversity transceiver through lake experiment, which was performed at Lake Kyungcheon, Mungyeong city using two transmitters and two receivers placed 460 m apart at an average depth of 40 m. The lake experiment results show that user data are recovered with error-free in both of the forward and reverse links.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.31 no.3A
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• pp.249-253
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• 2006

We propose a new beamforming algorithm, which is based on simplex downhill optimization method in the presence of pilot channels in cdma2000 reverse-link, for the rake structure antenna array in DS/CDMA communication system. Our approach uses the desired signal(pilot) covariance matrix and the interference covariance matrix. The beamforming weights are made according to maximum SINR criteria using simplex downhill optimization procedure. Our proposed scheme provides lower computational load, better convergence speed, better performance than existingadaptive beamforming algorithm. The simplex downhill method is well suited to finding the optimal or sub-optimal weight vector, since they require only the value of the deterministic function to be optimized. The rake beamformer performances are also evaluated under several set of practical parameter values with regard to spatial channel model. We also compare the performance between conventional rake receiver and the proposed one under same receiving power.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.27 no.5
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• pp.1049-1058
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• 2017

The rapid evolution of mobile technologies and proliferation of mobile devices have created a new channel for marketing by mobile advertising. As mobile advertising is a close relative to online advertising, it also has similar problems such as advertisement injections (Ad injections). Users are exposed to unwanted advertisements and redundant web traffic by injected ads can cause additional charges of mobile devices. Although mobile ad injection can cause many problems it has been merely studied. In this paper, we analyze ad injection activities by mobile applications that exploit a legitimate application (Naver mobile application). We reverse-engineered 2 mobile applications and find out characteristics of mobile ad injections. We compare mobile ad injections with online ad injections and suggest feasible mitigations.

• Membrane Journal
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• v.7 no.2
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• pp.75-83
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• 1997

The disc plate and frame type modules for reverse osmosis were developed using three different types of baffles: linear (Type 1), curved (Type 2) and parallel shapes (Type 3). Separation performance tests were carried out for the modules using NaCl and sucrose solutions under the various concentrations and operating pressures. The permeation flux and solute rejection ratio for Type 3 module were the highest within operating pressure (35bar) and flow rate (6 l/min). The flux improvement ratio of Type 2 or 3 to Type 1 for NaCl solution decreased as operating pressure increased: flux improvement ratios of Type 3 for 1wt% of NaCl solution were about 100 and 10% at 10 and 35bar, respectively. However, the flux improvement ratio for sucrose solutions varied with the operating pressure and concentration. The permeation flux for Type 3 depended on the flow rate linearly, which is higher than that of turbulent flow region in the smooth channel.

• Korean journal of applied entomology
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• v.43 no.2
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• pp.155-162
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• 2004

Serotonin receptor binds to serotonin (5-HT) and activates effector proteins such as adenylyl cyclase, phospholipase C, cyclic GMP phosphodiesterase or ion channel through G protein on the cell membrane, resulting in various physiological responses like diuresis, memory and development. To examine the comparative expression of the 5-HT$\_$7/ receptor of Aedes aegypti, the Aedes 5-HT$\_$7/ receptor gene was transfected into Drosophila Schneider2 (S2) cells and mammalian Chinese hamster ovary (CHO)-Kl cells. The expression of the Aedes 5-HT$\_$7/ receptor gene in selected cell lines, Tr-CHO and Tr-S2, was confirmed with reverse transcription-PCR, Western blot and immunocytochemistry. Compared with the induced intracellular cAMP level of Tr-S2 cell line to 5-HT, the induced cAMP in the Tr-CHO cell line was over 9 times higher and was dose-dependent. These results suggest that the functionality of Aedes 5-HT$\_$7/ receptor is much more effective in mammalian CHO-K 1 cells and that the Tr-CHO cell line expressing Aedes 5-HT$\_$7/ receptor can be used for synthetic agonist or antagonist candidate screening.

• Transactions of the KSME C: Technology and Education
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• v.3 no.3
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• pp.217-223
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• 2015

The patterning of microbead in microfluidics channel is a practical technique for application in bio and medical areas. An approach is described for a direct patterning of magnetically active microbeads in microfluidic devices without inner structure. Local magnet arrangements - flat arrangement and stack arrangement - contacting same poles or opposite poles of magnet were utilized for generating trapping magnetic fields. The arrangement of magnets contacting same poles generated isolated patterns by repelling of magnetic field. The flat arrangement of vertically reverse magnet arrays shaped trapping patterns repelling magnetic field line between same poles. Spatially, the stack compositions of magnet arrangements allow diverse isolated trapped patterns of magnetic particles. Trapped magnetic particles in fluidic channels were stable on the $18m{\ell}/hr$ flow conditions and magnetic force of 1.08 mT in the all experiments. This experimental study suggests the simple and versatile methods to pattern magnetic particles, and has potential of wide application to bio and medical area.

• Proceedings of the Korean Vacuum Society Conference
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• 2013.08a
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• pp.273-273
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• 2013

Recently, Point-of-care (POC) testing microdevices enable to do the patient monitoring, drug screening, pathogen detection in the outside of hospital. Immunochromatographic strip (ICS) is one of the diagnostic technologies which are widely applied to POC detection. Relatively low cost, simplicity to use, easy interpretations of the diagnostic results and high stability under any circumstances are representative advantages of POC diagnosis. It would provide colorimetric results more conveniently, if the genetic analysis microsystem incorporates the ICS as a detector part. In this work, we develop a reverse transcriptase-polymerase chain reaction (RT-PCR) microfluidic device integrated with a ROSGENE strip for colorimetric influenza H1N1 virus detection. The integrated RT-PCR- ROSGENE device is consist of four functional units which are a pneumatic micropump for sample loading, 2 ${\mu}L$ volume RT-PCR chamber for target gene amplification, a resistance temperature detector (RTD) electrode for temperature control, and a ROSGENE strip for target gene detection. The device was fabricated by combining four layers: First wafer is for RTD microfabrication, the second wafer is for PCR chamber at the bottom and micropump channel on the top, the third is the monolithic PDMS, and the fourth is the manifold for micropump operation. The RT-PCR was performed with subtype specific forward and reverse primers which were labeled with Texas-red, serving as a fluorescent hapten. A biotin-dUTP was used to insert biotin moieties in the PCR amplicons, during the RT-PCR. The RT-PCR amplicons were loaded in the sample application area, and they were conjugated with Au NP-labeled hapten-antibody. The test band embedded with streptavidins captures the biotin labeled amplicons and we can see violet colorimetric signals if the target gene was amplified with the control line. The off-chip RT-PCR amplicons of the influenza H1N1 virus were analyzed with a ROSGENE strip in comparison with an agarose gel electrophoresis. The intensities of test line was proportional to the template quantity and the detection sensitivity of the strip was better than that of the agarose gel. The test band of the ROSGENE strip could be observed with only 10 copies of a RNA template by the naked eyes. For the on-chip RT-PCR-ROSGENE experiments, a RT-PCR cocktail was injected into the chamber from the inlet reservoir to the waste outlet by the micro-pump actuation. After filling without bubbles inside the chamber, a RT-PCR thermal cycling was executed for 2 hours with all the microvalves closed to isolate the PCR chamber. After thermal cycling, the RT-PCR product was delivered to the attached ROSGENE strip through the outlet reservoir. After dropping 40 ${\mu}L$ of an eluant buffer at the end of the strip, the violet test line was detected as a H1N1 virus indicator, while the negative experiment only revealed a control line and while the positive experiment a control and a test line was appeared.

• Journal of Periodontal and Implant Science
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• v.45 no.2
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• pp.69-75
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• 2015

Purpose: Salivary fluid formation is primarily driven by Ca2+-activated, apical efflux of chloride into the lumen of the salivary acinus. The anoctamin1 protein is an anion channel with properties resembling the endogenous calcium-activated chloride channels. In order to better understand the role of anoctamin proteins in salivary exocrine secretion, the expression of the ten members of the anoctamin gene family in the mouse submandibular gland was studied. Methods: Total RNA extracted from mouse submandibular salivary glands was reverse transcribed using primer pairs to amplify the full-length coding regions of each anoctamin gene and was subcloned into plasmid vectors for DNA sequencing. Alternative splice variants were also screened by polymerase chain reaction using primer pairs that amplified six overlapping regions of the complementary DNA of each anoctamin gene, spanning multiple exons. Results: Multiple anoctamin transcripts were found in the mouse submandibular salivary gland, including full-length transcripts of anoctamin1, anoctamin3, anoctamin4, anoctamin5, anoctamin6, anoctamin9, and anoctamin10. Exon-skipping splicing in the N-terminal exons of the anoctamins1, anoctamin5, and anoctamin6 genes resulted in multiple alternative splice variants. No expression of anoctamin2, anoctamin7, or anoctamin8 was found. Conclusions: The predominant anoctamin transcript expressed in the mouse submandibular gland is anoctamin1ac. The chloride channel protein produced by anoctamin1ac is likely responsible for the $Ca^{2+}$-activated chloride efflux, which is the rate-limiting step in salivary exocrine secretion.

• Journal of Sensor Science and Technology
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• v.7 no.6
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• pp.401-408
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• 1998

A dual-channel automatic ac-dc voltage transfer error measurement system in which the output voltages of two thermal voltage converters which are ac voltage standard are directly measured at the same time to reduce the output voltage drift is described. Forward-reverse measurement method by using a two-channel scanner is used to cancel the offset voltage of the voltmeters. The agreements of the 4-V TVC comparison results between other national standards institute and Korea Research Institute of Standards and Science were less than about ${\pm}2\;ppm$ in the frequency range of $40\;Hz{\sim}100\;kHz$, and were less than about ${\pm}4\;ppm$ at $200\;kHz{\sim}1\;MHz$. Measurement uncertainty is reduced significantly from ${\pm}4\;ppm$ of manual system to ${\pm}3\;ppm$ of new system(up to 100 kHz) typically and great increase in comparison efficiency has been achieved by this system.

• Journal of Broadcast Engineering
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• v.14 no.2
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• pp.189-196
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• 2009

We propose a hybrid broadcast scheduling based on a combination of periodic and on-demand data scheduling methods for mobile data broadcasting in convergence networks from communication and broadcasting. We consider an environment in which the forward channel is for data broadcasting and the reverse channel is for sending data requests via cellular phones, WLAN, WiBro, etc. Collecting statistics of requests from clients, the server partitions the data items into hot-item and cold-item sets. Hot items are sent based on a push-based scheduling. An on-demand scheduling method is applied to cold items. Performance evaluation from simulations shows that our proposed scheduling algorithm yields small response time with high successful response ratio.