Browse > Article

Comparative Expression of the Aedes aegypti 5-Hydroxytryptamine7 Receptor in Drosophila Schneider2 and Chinese Hamster Ovary-K1 Cells  

이대원 (서울대학교 농업생명과학대학 농생명공학부)
Publication Information
Korean journal of applied entomology / v.43, no.2, 2004 , pp. 155-162 More about this Journal
Abstract
Serotonin receptor binds to serotonin (5-HT) and activates effector proteins such as adenylyl cyclase, phospholipase C, cyclic GMP phosphodiesterase or ion channel through G protein on the cell membrane, resulting in various physiological responses like diuresis, memory and development. To examine the comparative expression of the 5-HT$\_$7/ receptor of Aedes aegypti, the Aedes 5-HT$\_$7/ receptor gene was transfected into Drosophila Schneider2 (S2) cells and mammalian Chinese hamster ovary (CHO)-Kl cells. The expression of the Aedes 5-HT$\_$7/ receptor gene in selected cell lines, Tr-CHO and Tr-S2, was confirmed with reverse transcription-PCR, Western blot and immunocytochemistry. Compared with the induced intracellular cAMP level of Tr-S2 cell line to 5-HT, the induced cAMP in the Tr-CHO cell line was over 9 times higher and was dose-dependent. These results suggest that the functionality of Aedes 5-HT$\_$7/ receptor is much more effective in mammalian CHO-K 1 cells and that the Tr-CHO cell line expressing Aedes 5-HT$\_$7/ receptor can be used for synthetic agonist or antagonist candidate screening.
Keywords
Serotonin receptor; Aedes aegypti; cAMP; Serotonin; Immunocytochemistry;
Citations & Related Records
연도 인용수 순위
  • Reference
1 Barnes, N.M. and T. Sharp. 1999. A review of central 5-HT receptors and their function. Neuropharmacol. 38: 1083~1152   DOI   ScienceOn
2 Coast, G.M. 1996. Neuropeptides implicated in the control of diuresis in insects. Peptides 17: 327~336   DOI   PUBMED   ScienceOn
3 Coast, G.M. 1998. Insect diuretic peptides: structures, evolution and actions. Am. Zool. 38: 442~449
4 Colas, J.-F., J.-M Launay and L. Maroteaux. 1999. Maternal and zygotic control of serotonin biosynthesis are both necessary for Drosophila germband extension. Mech. Dev. 87: 67~76   DOI   PUBMED   ScienceOn
5 Giles, H., S.J. Lansdell, M.L. Bolofo, H.L. Wilson and G.R. Martin. 1996. Characterization of a 5-$HT_IB$ receptor on CHO cells: functional responses in the absence of radioligand bind-ing. Br. J. Pharmacal. 117: 1119-1126   DOI   ScienceOn
6 Lange, A.B., I. Orchard and F.M. Barrett. 1989. Changes in haemolymph serotonin levels associated with feeding in the blood sucking bug, Rhodnius prolixus. J. Insect Physiol. 35: 393~399   DOI   ScienceOn
7 Nassel, D.R. 1996. Neuropeptides, amines, and amino acids in an elementary insect ganglion: functional and chemical anatomy of the unfused abdominal ganglion. Prog. Neurobiol. 48: 325-420   DOI   ScienceOn
8 Ohtaki, T., K. Ogi, Y. Masuda, K. Mitsuoka, Y. Fujiyoshi, C. Kitada, H. Sawada, H. Onda and M. Fujino. 1998. Expression, purification, and reconstitution of receptor for pituitary adeny-late cyclase-activating polypeptide: Large-scale purification of a functionally active G protein-coupled receptor produced in Sf9 insect cells. J. BioI. Chem. 273: 15464~15473   DOI   ScienceOn
9 Pietrantonio, P.V., C. Jagge and C. McDowell. 2001. Cloning and expression analysis of a 5$HT_7$-like serotonin receptor cDNA from mosquito Aedes aegypti female excretory and respiratory systems. Insect Mol. BioI. 10: 357~369   DOI   ScienceOn
10 Pietrantonio, P.V., G. Gibson, D. Petzel, A. Strey and T.K. Hayes. 2000. Characterization of a leucokinin binding protein in Aedes aegypti (Diptera: Culicidae) Malpighian tubule. Insect Bio-chem. Mol. BioI. 30: 1147~1159   DOI   ScienceOn
11 Schooley, D.A. 1991. Chemical identification of insect diuretic peptides. pp. 83~94. In Insect Neuropeptides: Chemistry, Bio-logy and Action, eds. by J.J. Menn, T.J. Kelly and E.P. Malser. 453pp. American Chemical Society, Columbus, OH
12 Towbin, H.R., R. Stachelin and J. Gordon. 1979. Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: Procedure and some applicatin. Proc. Natl. Acad. Sci. USA 76: 4350-4354   DOI   ScienceOn
13 Yu, M.J. and K.W. Beyenbach. 2002. Leucokinin activates $CA^2+$ -dependent signal pathway in principal cells of Aedes aegypti Malpighian tubules. Am. J. Physiol. 283: F499-F508
14 Valles, A.M. and K. White. 1988. Serotonin-containing neurons in Drosophila melanogaster: development and distribution. J. Compo Neurol. 268: 414~428   DOI   ScienceOn
15 Novak, M.G. and W.A. Rowley. 1994. Serotonin depletion affects blood-feeding but not host-seeking ability in Aedes triseriatus (Diptera: Culicidae). J. Med. Entomol. 31: 600-606
16 Colas, J.-F., J.-M. Launay, O. Kellermann, P. Rosay and L. Maroteaux. 1995. Drosophila 5-$HT_2$ serotonin receptor: coex-pression with fushi-tarazu during segmentation. Proc. Natl. Acad. Sci. USA 92: 5441-5445   DOI   ScienceOn
17 Vanden Broeck, J. 2001. Insect G protein-coupled receptors and signal transduction. Arch. Insect Biochem. Physiol. 48: 1~12   DOI   PUBMED   ScienceOn
18 Clark, T.M. and T.J. Bradley. 1997. Malpighian tubules of larval Aedes aegypti are hormonally stimulated by 5-hydroxytryp-tamine in response to increased salinity. Arch. Insect Biochem. Physiol. 34: 123~141   DOI   ScienceOn
19 Kozak, M. 1987. An analysis of 5'-noncoding sequences from 699 vertebrate messenger RNAs. Nucl. Acids Res. 15: 8125-8148   DOI   PUBMED   ScienceOn
20 Roeder, T. 1994. Biogenic amines and their receptors in insects. Comp. Biochem. Physiol. 107C: 1~12   DOI
21 Butkerait, P., Y. Zheng, H. Hallak, T.E. Graham, H.A. Miller, K.D. Burris, P.B. Molinoff and D.R. Manning. 1995. Expres-sion of the human 5- hydroxytryptamine$_IA$ receptor in Sf9 cells. Reconstitution of a coupled phenotype by co-expression of mammalian G protein subunits. J. BioI. Chem. 270: 18691-18699   DOI   ScienceOn
22 Audsley, N., I. Kay, T.K. Hayes and G.M. Goast. 1993. The effect of Manduca sexta diuretic hormone on fluid transport by the Malpighian tubules and cryptonephric complex of Manduca sexta. J. Exp. BioI. 178: 231-243
23 Laemmli, U.K 1970. Cleavage of structural proteins during the assembly of the head of bacteriphage T4. Nature 227: 680~685   DOI   PUBMED   ScienceOn
24 Novak, M.G., J.M. Ribeiro and J.G. Hildebrand. 1995. 5-hydroxy-tryptamine in the salivary glands of adult female Aedes aegypti and its role in regulation of salivation. J. Exp. BioI. 198: 167-174
25 Clark, T.M., A. Koch and D.F. Moffett. 1999. The anterior and posterior 'stomach' regions of larval Aedes aegypti midgut: regional specialization of ion transport and stimulation by 5-hydroxytryptamine. J. Exp. BioI. 202: 247-252
26 Laurenza, A., E.M. Sutkowski and K.B. Seamon. 1989. Forskolin: a specific stimulator of adenylyl cyclase or a diterpene with multiple sites of action? Trends Pharmacol. Sci. 10: 442~447   DOI   ScienceOn
27 Coast, G.M., I. Orchard, J.E. Philips and D.A. Schooley. 2002. Insect diuretic and antidiuretic hormones. Adv. Insect Physiol. 29: 279~409   DOI
28 Hill, C.A., A.N. Fox, R.J. Pitts, L.B. Kent, P.L. Tan, M.A. Chrystal, A. Cravchik, F.H. Collins, H.M. Robertson and L.J. Zwiebel. 2002. G protein-coupled receptors in Anopheles gambiae. Science 298: 176~178   DOI   PUBMED   ScienceOn
29 Lehmberg, E., R. Ota, K. Furuya, D. King, S. Applebaum, H. Ferenz and D. Schooley. 1991. Identification of a diuretic hor-mone of Locusta migratoria. Biochem. Biophys. Res. Commun. 179: 1036~1041   DOI   ScienceOn
30 Schoofs, L., G.M. Holman, P. Proost, J. Van Damme, T.K. Hayes and A. De Loof. 1992. Locustakinin, a novel myotropic peptide from Locusta migratoria, isolation, primary structure and syn-thesis. Regul. Pept. 37: 49~57   DOI   ScienceOn
31 Gerhardt, C.C. and H. van Heerikhuizen. 1997. Functional charac-teristics of heterologously expressed 5-HT receptors. Eur. J. Pharmacol. 334: 1~23   DOI   PUBMED   ScienceOn
32 Adams, T.S. 1999. Hematophagy and hormone release. Ann. Entom. Soc. Am. 92: 1~13
33 Kay, I., G.M. Coast, O. Cusinato, C.H. Wheeler, N.F. Totty and G.J. Goldsworthy. 1991. Isolation and characterization of a diuretic peptide from Acheta domesticus, evidence for a family of insect diuretic peptides. Biochem. Chem. 372: 505~512
34 Lee, D.-W. and P.V. Pietrantonio. 2003. In vitro expression and pharmacology of the 5-$HT_7$-like receptor present in the mos-quito Aedes aegypti tracheolar cells and hindgut-associated nerves. Insect Mol. BioI. 12: 561~569   DOI   ScienceOn
35 Holman, G.M., R.J. Nachman and G.M. Coast. 1999. Isolation, characterization and biological activity of a diuretic myokinin neuropeptide from the housefly, Musca domestica. Peptides 20: 1~10   DOI   ScienceOn
36 Saudou, F., U. Boschert, N. Amlaiky, J.-L. Plassat and R. Hen. 1992. A family of Drosophila serotonin receptors with distinct intracellular signaling properties and expression patterns. EMBO J.11: 7-17
37 Veenstra, J.A. 1988. Effects of 5-hydroxytryptamine on the Malpighian tubules of Aedes aegypti. J. Insect Physiol. 34: 299~304   DOI   ScienceOn
38 Bicker, G. and R. Menzel. 1989. Chemical codes for the control of behaviour in arthropods. Nature 337: 33-39   DOI   ScienceOn
39 Clark, T.M. and T.J. Bradley. 1996. Stimulation of Malpighian tubules from larval Aedes aegypti by secretagogues. J. Insect Physiol. 42: 593~602   DOI   ScienceOn
40 Witz, P., N. Amlaiky, J.-L. Plassat, L. Maroteaux, E. Borrelli and R. Hen. 1990. Cloning and characterization of a Drosophila serotonin receptor that activates adenylate cyclase. Proc. Natl. Acad. Sci. USA 87: 8940-8944   DOI   ScienceOn
41 Boundy, V.A., L. Lu and P.B. Molinoff. 1996. Differential coupl-ing of rat D2 dopamine receptor isoforms expressed in Spodop-tera frugiperda insect cells. J. Pharmacol. Exp. Ther. 276: 784-794
42 Seamon, K.B., W. Padgett and J.W. Daily. 1981. Forskolin: unique diterpene activator of adenylate cyclase in membranes and intact cells. Proc. Natl. Acad. Sci. USA 78: 3363-3367   DOI   ScienceOn