• Title/Summary/Keyword: Reverse

Search Result 7,779, Processing Time 0.032 seconds

A Study on Reverse Design of Cam Mechanism using NURBS (NURBS를 이용한 캠 기구의 역설계에 관한 연구)

  • 김상진;신중호;김대원;윤호업
    • Proceedings of the Korean Society of Precision Engineering Conference
    • /
    • 2002.10a
    • /
    • pp.920-924
    • /
    • 2002
  • This paper presents the reverse design of a cam mechanism using NURBS(Nonuniform Rational B-spline curve). Cam is very difficult to make the accurate shape on the design and the manufacture. Because the cam shape is commonly made in order to move in special functions. The reverse design can be used to check accuracy between the designed data and the manufactured data of the cam shape and also reproduce the cam without the design data. The reverse design procedures consist of motion analysis and curve fitting. The motion analysis is used the central difference method and the relative velocity method to find the displacement and velocity. The curve fitting is used NURBS to develope the whole curve. The central difference method is derived in the 3 dimensional space.

  • PDF

Effect of Reverse Transformation on the Microstructure and Retained Austenite Formation of 0.14C-6.SMn Alloy Steel (0.14C-6.5Mn 합금강의 미세조직과 잔류오스테나이트 형성에 미치는 역변태처리의 영향)

  • Song, K.H.;Lee, O.Y.
    • Journal of the Korean Society for Heat Treatment
    • /
    • v.13 no.4
    • /
    • pp.253-258
    • /
    • 2000
  • The present study aimed to develop the TRIP(transformation induced plasticity) aided high strength low carbon steel sheets using reverse transformation process. The cold-rolled 0.14C-6.5Mn steel was reverse-transformed by slow heating to intercritical temperature region and air cooling to room temperature. An excellant combination of tensile strength and elongation of $98.3kgf/mm^2$ and 44.4% appears. This combination comes from TRIP phenomena of retained austenite during deformation. The stability of retained austenite Is very Important for the good ductility and it depends on diffusion of carbon and manganese during reverse transformation. The air cooling after holding at intercritical temperature retards the formation of pearlite and provides the carbon enrichment in retained austenite, resulting the increase of elongation in cold-roiled TRIP steel.

  • PDF

Proteolysis of the Reverse Transcriptase of Hepatitis B Virus by Lon Protease in E. coli

  • Han, Joo-Seok;Park, Jae-Yong;Hwang, Deog-Su
    • Animal cells and systems
    • /
    • v.5 no.3
    • /
    • pp.195-198
    • /
    • 2001
  • Hepatitis B virus (HBV) polymerase, which possesses the activities of terminal binding, DNA polymerase, reverse transcriptase and RNaseH, has been shown to accomplish viral DNA replication through a pregenomic intermediate. Because the HBV polymerase has not been purified, the expression of HBV polymerase was examined in an E. coli expression system that is under the regulation of arabinose operon. The expressed individual domain containing terminal binding protein, polymerase, or RNaseH turned out to be insoluble. The activities of those domains were not able to be recovered by denaturation and renaturation using urea or guanidine-HCI. The expressed reverse transcriptase containing the polymerase and RNaseH domains became extensively degraded, whereas the proteolysis was reduced in a Ion- mutant. These results indicate that Lon protease proteolyzes the HBV reverse transcriptase expressed in E. coli.

  • PDF

SANS Studies on the Formation of PANI Nanoparticles in the Reverse Micelles

  • Sim, Jae-Hyun;Kim, Myung-woong;Park, Sang-wook;Bang, Jeong-Hwa;Sohn, Dae-won
    • Bulletin of the Korean Chemical Society
    • /
    • v.27 no.2
    • /
    • pp.251-254
    • /
    • 2006
  • The formation of polyaniline (PANI) in the reverse micelles of poly(oxyethylene) nonylphenyl ether, $(NP5, H(CH_2)_9Ph(OC_2H_4)_5OH)$, was investigated by small-angle neutron scattering (SANS). The reverse micellar solution containing initiators in the inner part of reverse micelle was prepared with surfactant (NP5), water, cyclohexane and an initiator (ammonium persulfate (APS)). The core-shell sphere model containing smearing effect reveals that the polymerization occurs on the shell layer of the reverse micelles. Shell thickness averages varied from 48 $\AA$ to 109 $\AA$ with increases of monomer concentration.

A Study on Development of Worker Performance Evaluation System in the Reverse Logistics (회수물류 시스템에서의 작업자 수행도 평가 시스템 개발 연구)

  • Yang, Kwang-Mo;Yun, Jun-Sub;Kang, Kyong-Sik
    • Proceedings of the Safety Management and Science Conference
    • /
    • 2006.11a
    • /
    • pp.287-292
    • /
    • 2006
  • Reducing the logistic cost may be accomplished by numbers of logistic management methods, but the most fundamental and essential one is the accomplishment of the consistent reverse logistics system that is the core of worker performance system, and the purpose of consistent reverse system is the treatment of logistic functions such as transportation, storage and unloading with consistent logistics, and increasing the turnover ratio is required for the improvement of the system. As the turnover ratios is increased, this paper make reverse worker performance evaluation system with lifetime value include of weight variables.

  • PDF

Back-Extraction Processes of C.C.Lipase with Mediated AOT Reverse Micellar System

  • Lee, Sung-Sik;Kim, Bong-Gyu;Sung, Nak-Chang;Lee, Jong-Pal
    • Bulletin of the Korean Chemical Society
    • /
    • v.25 no.6
    • /
    • pp.873-877
    • /
    • 2004
  • The relationship between the behaviors of c.c.lipase back-extraction and their percolation phenomena by using AOT reverse micellar systems (RVMS) has been studied by the addition of a small amount of additives to organic phase such as thiols and nonionic-surfactants focusing on micelle-micelle interactions. The values of ${\beta}_t$ defined by the variation of percolation processes and back-extraction behaviors of c.c.lipase have a good linear correlation. The hydrophobicity of additive molecules suppressing the cluster formation of reverse micelles (high values of ${\beta}_t$) improved the back-extraction behavior of c.c.lipase. The back-extraction fraction and its rate of c.c.clipase are increased with decreasing of the value of hydrophilic lipophilic balance (HLB) and increasing of the hydrophobicity per additive molecules added to reverse micellar systems (RVSM) in the same additives concentration.

A Simple Method for Elimination of False Positive Results in RT-PCR

  • Martel, Fatima;Grundemann, Dirk;Schomig, Edgar
    • BMB Reports
    • /
    • v.35 no.2
    • /
    • pp.248-250
    • /
    • 2002
  • Discrimination between the amplification of mRNA and contaminating genomic DNA is a common problem when performing a reverse transcriptase-polymerase chain reaction (RT-PCR). Even after treatment of the samples with DNAse, it is possible that negative controls (samples in which no reverse transcriptase was added) will give positive results. This indicates that there was amplification of DNA, which was not generated during the reverse transcriptase step. The possibility exists that Taq DNA polymerase acts as a reverse transcriptase, generating cDNA from RNA during the PCR step. In order to test this hypothesis, we incubated samples with a DNAse-free RNAse after the cDNA synthesis. Comparison of the results that were obtained from these samples (incubated with or without DNAse-free RNAse) confirms that the reverse transcriptase activity of Taq DNA polymerase I is a possible source of false positive results when performing RT-PCR from intronless genes. Moreover, we describe here a simple and rapid method to overcome the false positive results that originate by this activity of Taq polymerase.

Study on the reverse engineering and performance test in the development of screw flowmeter (스크류유량계 개발에 있어서의 역공학 및 성능평가에 관한 연구)

  • Kim Jong-Yoon;Hwang Jong-Dae;Lee Sang-Ryul;Jung Yoon-Gyo
    • Proceedings of the Korean Society of Machine Tool Engineers Conference
    • /
    • 2005.05a
    • /
    • pp.132-137
    • /
    • 2005
  • This research presents a modeling and a manufacturing method of screw type flow meter. This paper introduces the efficient design and manufacturing method of screw type flow meter using reverse engineering and test technology. The methods introduced this paper utilize the reverse engineering that is increasing accuracy of modeling and manufacturing of reverse model. And then it can be used in performance test with hydraulic test equipment. Hence this can be used in the basic document for development of the quite accurate flow meter.

  • PDF

The study of logistics optimization model with empty transfer rate of reverse logistics (역물류의 공차율을 고려한 물류 최적화 모델 연구)

  • Ryu, Byung-Woo;Park, Jae-Hyun
    • Journal of the Korea Safety Management & Science
    • /
    • v.8 no.3
    • /
    • pp.125-141
    • /
    • 2006
  • If do not become whole optimization because enterprise's individual enterprise internal determinate improvement effort is associated with activity in enterprise SCM, competitive power can not but be extremely limited the result. Therefore, to spur in hidden cost's discovery and logistics' optimization is actuality. In this paper, as long as it is by logistics cost except empty driving if reverse logistics happens in distribution channel progressing, logistics reverse logistics' hidden cost through model that do minimization profit of logistics to look for plan that can do maximization try. Finally, is based on dual of distribution cost and profit of logistics and reverse logistics and achieves Pull, optimal system modeling that use Push system and gropes method of effective logistics cost.

The Measurement of Properties for Elastic-Plastic Material by Using Spherical Indentation and Finite Element Analysis (Spherical Indentation 실험과 유한요소 해석기법을 이용한 탄소성 물성치 측정)

  • Li, Guang-He;Chen, Xi;Park, Tae-Hyo
    • Proceedings of the Computational Structural Engineering Institute Conference
    • /
    • 2010.04a
    • /
    • pp.268-271
    • /
    • 2010
  • In this paper, forward and reverse analysis is introduced in order to estimate the elastic-plastic properties from a power-law hardening bulk specimen materials with one simple spherical indentation impression test. In order to verify the reliability of the reverse analysis, we have simulated about a large range of materials that essentially cover all engineering materials, using ABAQUS(6.91) program. Then, we could obtained the fitting functions and plastic parameters from the numerical analysis results. Next, through the procedure of reverse analysis we can obtain the yield stress and power-law exponent. Finally, obtain good agreement between the result from reverse analysis and initial input data from experiment.

  • PDF