• 한국토양비료학회지
• /
• 제49권2호
• /
• pp.144-156
• /
• 2016

Soil is a dynamic biological system, in which it is difficult to determine the composition of microbial communities. Knowledge of microbial diversity and function in soils are limited because of the taxonomic and methodological limitations associated with studying the organisms. In this review, approaches to measure microbial diversity in soil were discussed. Research on soil microbes can be categorized as structural diversity, functional diversity and genetic diversity studies, and these include cultivation based and cultivation independent methods. Cultivation independent technique to evaluate soil structural diversity include different techniques such as Phospholipid Fatty Acids (PLFA) and Fatty Acid Methyl Ester (FAME) analysis. Carbon source utilization pattern of soil microorganisms by Community Level Physiological Profiling (CLPP), catabolic responses by Substrate Induced Respiration technique (SIR) and soil microbial enzyme activities are discussed. Genetic diversity of soil microorganisms using molecular techniques such as 16S rDNA analysis Denaturing Gradient Gel Electrophoresis (DGGE) / Temperature Gradient Gel Electrophoresis (TGGE), Terminal Restriction Fragment Length Polymorphism (T-RFLP), Single Strand Conformation Polymorphism (SSCP), Restriction Fragment Length Polymorphism (RFLP) / Amplified Ribosomal DNA Restriction Analysis (ARDRA) and Ribosomal Intergenic Spacer Analysis (RISA) are also discussed. The chapter ends with a final conclusion on the advantages and disadvantages of different techniques and advances in molecular techniques to study the soil microbial diversity.

• Journal of Forest and Environmental Science
• /
• 제37권1호
• /
• pp.69-78
• /
• 2021

This study aims to verify a difference between the years 2014 and 2019 of Uiryeong-Gil, which is operated by the Visitor-Reservation System, in terms of the cognition of system, operational effectiveness, and behavioral intention. The analysis is as follows. First, among the overall cognition of the Visitor-Reservation System, the satisfaction from - prevention for safety for accident, expansion to other parks, number of visitor restriction, and satisfaction with reservation method - increased compared to 2014. Second, among the effects of the Visitor-Reservation System operation, satisfaction shows differences in year-to-year levels in terms of resource protection, resource damage mitigation efficiency, natural experience opportunity, comfort of visit-environment, and prevention of illegal activities. Third, the analysis of the differences in the behavior of the Visitor-Reservation System showed that the satisfaction of visitors, revisit intention, and overall satisfaction differed per year. The results of this study are meaningful in that the Visitor-Reservation System is no longer the restriction, but the change in cognition for the protection of ecosystems and sustainable visiting in order to expand the Visitor-Reservation System into a sustainable policy.

• 한국전문물리치료학회지
• /
• 제28권1호
• /
• pp.13-17
• /
• 2021

A normal range of motion is essential for performing activities of daily living. The capsular pattern is the proportional motion restriction in range of motion during passive exercises due to tightness of the joint capsule. Although the capsular pattern is widely referred to in clinical practice, there is no scientific evidence to support the concept. In this review, the appropriateness of the capsular pattern for evaluation of joint pathology was assessed. In the Textbook of Orthopaedic Medicine written by Cyriax, the capsular pattern did not specify how much reduction in angular motion is considered motion restriction. As the definition proposed initially was unclear, different methods have been used in previous studies investigating capsular pattern. In addition, the capsular pattern described all the major joints of the human body, but only the hip joint, knee joint, and shoulder joint were studied in experimental studies. Sensitivity and specificity were reported in one study and were meaningful in specific pathologies (loss of extension to loss of flexion). There was no consensus on the reliability and validity. In summary, the capsular pattern suggested by Cyriax or Kaltenborn is not supported or applies only to certain conditions. Various components around a joint complement each other and provide stability to the joint. It is recommended that the therapist perform multiple assessments rather than rely on a single assessment when evaluating joints.

• The Plant Pathology Journal
• /
• 제21권1호
• /
• pp.13-20
• /
• 2005

Chlorella viruses are large icosahedral, plaque-forming, dsDNA viruses that infect certain unicellular, chlorellalike green algae. The genomic DNA of over 300 kb contains many useful genes and promoters. Over 40 chlorella viruses have been isolated from fresh water in Korea since 1998. The viruses were amplified initially in chlorella strain NC64A, and pure isolates were obtained by repeated plaque isolation. SDS-PAGE analysis revealed similar but distinct protein patterns, both among the group of purified viruses and in comparison with the prototype chlorella virus PBCV-1. Digestions of the 330- to 350-kb genomic DNAs with 10 restriction enzymes revealed different restriction fragment patterns among the isolates. The tRNA-coding regions of 8 chlorella viruses were cloned and sequenced. These viruses contain 14-16 tRNA genes within a 1.2- to 2-kb region, except for the SS-1 isolate, which has a 1039-bp spacer in a cluster of 11 tRNA genes. Promoter regions of several early genes were isolated and their activities were analyzed in transformed chlorella. Some promoters showed stronger activity than commonly used CaMV 35S promoter and chlorella transformation vectors for heterologous protein are beings constructed using these promoters.

• 한국정보통신학회:학술대회논문집
• /
• 한국정보통신학회 2019년도 춘계학술대회
• /
• pp.142-145
• /
• 2019

최근 들어 지구 온난화 및 고유가로 인한 에너지 위기로 전 세계적으로 이산화탄소 배출규제가 본격화되고 에너지 소비에 따른 지구환경을 보존하기 위한 대표적인 국제적인 GEF(Green Energy Family) 활동은 이산화탄소 배출 금지를 위한 교토의 정서(Kyoto protocol), RoHS(Restriction of Hazardous Substances directive)에서는 무 수은 조명 사용억제, WEEE(Waste Electrical and Electronice Equipment)에서는 조명 통신융합으로 폐기물 최소화를 목적으로 폐기물 회수를 요구하는 등 다각적 노력을 경지하고 있다. 본 논문에서는 기존의 외부 환경 요인(조도, 피사체와의 거리 등)에 의하여 실시간으로 변동되는 환경 데이터를 마이크로프로세서를 활용하여 외부 환경 요인을 확인하고 또한 퍼지 추론 시스템을 접목하여 RGB LED 모듈 조명 제어가 가능한 제어기를 구성하였다.

• 한국보건간호학회지
• /
• 제15권2호
• /
• pp.324-333
• /
• 2001

The purpose of this cross-sectional study was to explore fear of falling in relation to activity restriction of in the community dwelling elderly. Two hundred ninety-nine community dwelling elderly residing in Seoul and Kyonggi-do were interviewed using a structured questionnaire. The results were as follows : 1. Subjects were predominantly women$(70.6\%)$, an average 72.5 years old; the incidence of falls in last one year was $45.5\%$. 2. The highest level of fear found for activity in those who say they do engage and those who do not engage in the activity was 'going out when it is slippery '(m=2.53, 2.81) and 'reach for something over your head'(m=1.66, 2.60). Fear of falling scores were significant lower for those who say they do engage in eight kinds of activities (excluded activities of given were 'go to the store', 'take a tub bath', 'get out of bed') than for those who do not engage in the activities. 3. Among those who did not perform the activity, the three activities, when it is slippy(n=80), reaching overhead(n=70), and walk outside(n=59),were most often avoided because fear alone as well as for reasons in addition to fear. 4. The significant variables associated with fear of falling were female(p=0.0000), 1 educational status(p=0.000l), poor economic condition(p=0.0007), and experience of falls in last one year(p=0.0007). Fear of falling is common in the community dwelling elderly in Korea and is associated with several demographical variables. Therefore, to prevent falls and to reduce fear of falling, further study for development of education program for the elderly is needed.

• 한국경제지리학회지
• /
• 제16권2호
• /
• pp.198-217
• /
• 2013

본 연구에서는 원격진료 이용자 행태를 시공간 재구조 관점에서 살펴보았고, 원격진료의 양면적 성격, 즉 확장성과 제약성에 관해 고찰하였다. 연구 결과에 의하면, 원격진료의 도입은 직접적인 온라인 서비스의 수혜자인 환자의 행태 변화에 영향을 끼치나, 이는 원격진료 이전의 일반적 의료 행태와 확연한 차이를 보인 것은 아니다. 이와 더해, 의원급 병원의 의료진 및 원격진료 최종 서비스 제공자인 토가네현립병원의 의료진의 행태에는 거의 영향을 주지 않는 것으로 나타났다. 원격진료의 도입으로 인해 의료기관에로의 접근성 및 효용성의 향상에 도움이 된 것은 사실이나, 여전히 원격진료는 최소한 한번 이상의 직접 진료를 요구하고 있고, 인간의 생명과 직접적으로 연계됨에도 불구하고 불완전한 기술적 특성을 가지고 있으며, 원격진료 이용에 있어서의 의사-환자, 의사 사이의 합의 문제로 인해 시공간적 제약성을 내포하게 된다. 결과적으로, 당초 원격진료가 처음 고안되었을 당시 많은 이용자들에게 주었던 기대감(확장성)과 달리, 오늘날의 원격진료는 시공간을 초월한 개념이라기보다는 기술, 사회, 문화, 경제 등의 다양한 요인으로 인해 제약성을 내포한 형태로 발전하고 있다고 할 수 있다.

• 국제지역연구
• /
• 제12권2호
• /
• pp.27-53
• /
• 2008

이 논문은 세계 각국 은행들의 비전통적 수익활동의 수준을 결정하는 다양한 요인을 조사한 논문이다. 구체적으로 이 논문은 여러 국가의 광범위한 자료를 사용하여 비이자수익 수준을 결정하는 은행 고유요인과 나라별 특성요인을 검토하였다. 이 논문은 전통적인 미국중심 은행들에만 국한된 기존의 연구를 넘어선 것에 초점을 두고 있으며 세계 각국 은행들의 세부적인 자산부채관리에 대한 정보를 제공하고 있다. 이 논문의 결과는 은행의 크기, 대손충당금의 수준, 명시적 예금보험의 존재, 은행업에 대한 제한의 정도, 각국 은행의 자유도, 정부 그리고 외국인 소유지분의 크기, 각국의 지배구조 정도 즉 투명성이 은행의 비이자수익 수준을 결정하는데 양의 영향을 미치고 있음을 제시하고 있다. 전통적 대출과 자금조달에 대한 더 큰 의존성, 시장중심의 경제체제, 해당국에 다수의 감독당국의 존재 등은 비이자수익과 음의 관계를 가지고 있다. 하지만 이 논문은 해당국가의 경제발전수준은 이러한 변수들의 관련성의 크기에 유의하게 영향을 미치고 있음을 또한 제시하고 있다.

• 보건의료산업학회지
• /
• 제13권3호
• /
• pp.127-136
• /
• 2019

Objectives: This study examined the determinants of quality of life, depending on the presence or absence of asthma in adults, based on secondary wave data. Methods: Among the 21,724 people participating in the 6th Korea National Health and Nutrition Examination Survey as it was conducted from the first to third period, 495 participants who were aged 19 or older and responded to the question of the presence or absence of asthma were included in the final analysis. Demographic characteristics were examined using the SPSS/WIN 23.0 software tool for analysis of complex sample survey data. Health-related characteristics were presented using descriptive and multivariate analysis of data. Rao_Scott ${\chi}^2$ was used for the analysis of differences in quality of life, and multiple regression analysis of complex sample survey data was used to analyze factors affecting quality of life. Results: The variable factors negatively influencing quality of life were aging, cognition of their ill health, and limited activities. Conclusions: Based on the analysis, the study suggests that practical and ongoing nursing intervention proposals to improve the quality of life of asthmatic patients should be implemented not only for physical limitations and aging but also for psychological factors that reflect subjective health statuses.

• 보건교육건강증진학회지
• /
• 제33권3호
• /
• pp.71-82
• /
• 2016

Objectives: In this study, it was analyzed which marketing activity strategy multinational tobacco companies established against Republic of Korea through their internal documents and it was checked out how the marketing activities mentioned in the documents turned out to be actually. Methods: For that, analyzed the internal documents of tobacco companies after connecting Legacy Tobacco Documents Library(LTDL). Three documents were selected finally out of total searched 1,752 documents except the ones that did not include the theme or not appropriate. Results: Internal documents analysis showed that people smoke because the filter of cigarette shown in the movies looks nice. Tobacco companies carried out the marketing through sponsorship of soccer games, F1 Grand Prix was found in the internal documents. Conclusions: The legal restriction on marketing activities of tobacco companies should be enhanced by removing the articles of allowing advertisement and promotion for tobacco companies in Korean domestic law.