• 제목/요약/키워드: Restriction map

검색결과 126건 처리시간 0.029초

CORESTRICTION MAP ON BRAUER SUBGROUPS

  • CHOI, EUN-MI
    • 대한수학회논문집
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    • 제20권1호
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    • pp.35-49
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    • 2005
  • For an extension field K of k, a restriction homomorphism on Brauer k-group B(k) maps Brauer k-algebras to Brauer K- algebras by tensor product. A purpose of this work is to study the restriction map that sends radical (Schur) k-algebras to radical (Schur) K-algebras. And we ask an analogous question with respect to corestriction map on Brauer group B(K) that whether the corestriction map sends radical K-algebras to radical k-algebras.

Pseudomonas putida에 내재하는 Plasmid pKU10의 제한지도 (Restriction Map of the R Plasmid pKU10 in Pseudomonas putida)

  • 전성희;임영복;심웅섭;이영록
    • 미생물학회지
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    • 제29권4호
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    • pp.226-229
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    • 1991
  • In our laboratory a R plasmid pKU10 was isolated from Pseudomonas and its characteristics were investigated. In this study, as a basic work to improve its utility as a cloning vehicle, restriction patterns of pKU10 were analyzed for other various restriction enzymes in addition to restriction evdonucleases previously examined. As a result, pKU10 DNA has two cleavage sites for ClaI and HpaI, and three sites for AvaI. The restriction map of pKU10 was supplemented with AvaI, ClaI, and HpaI. From the result of this experiment, the usefulness of PKU10 as a cloning vector in Pseudomonas will be enhanced by constructions of mini-plasmid or hybrid plasmids.

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Restriction and transcription maps of mitochondrial DNA of trimorphomyces papilionaceus

  • Jeoung, Won-Jin;Hong, Soon-Gyu;Won, Kang-Young;Jung, Hack-Sung
    • Journal of Microbiology
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    • 제33권2호
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    • pp.149-153
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    • 1995
  • Mitochondrial DNA has been isolated from Trimorphomyces papilionaceus. By analyzing DNA fragments digested by restriction enzymes, a restriction site map has been constructured. The mtDNA of T. papilionaceus amounts to 48.5 kb in size and is circular in structure. Entire mitochondrial DNA was cloned in E coli plasmids and Northern blot hybridization was done using cloned and subcloned DNAs as probes. Based on hybridization results of mitochondrial RNA transcripts, a transcription map was prepared.

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다제내성 황색포도상구균이 가지고 있는 테트라사이클린 내성 플라스미드의 동정 (Characterization of Tetracycline Resistance Plesmid of Multidrug-resistant Staphylococcus aureus)

  • 이대운;문경호
    • 약학회지
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    • 제39권1호
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    • pp.6-9
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    • 1995
  • The clinical isolate Staphylococcus aureus SA2 had four kinds of plasmids and was resistant to ampicillin, chloramphenicol, clindamycin, erythromycin, gentamicin, kanamycin, methicillin, streptomycin, tetracycline and tobramycin. Transformation experiment demonstrated that 4.44 kb plasmid(pKH6) encoded resistance to tetracycline. The cleavage map of pKH6 was determined by restriction enzyme mapping techniques. The cleavage map is given for EcoRV, HindIII, HpaI, HpaII, KpnI and Xbal. Restriction endonucleases BamHl, BglI, BGIII, BstEII, EcoRI, HaellI, PstI, PvuII, SalI, Smal, and Xhol have no site on this plasmid. The restriction map revealed extensive structural homology between pKH6 and pT181.

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정사영상을 이용한 토지경계설정 실험 (Tests of Land Boundary Using Orthoimages)

  • 이영진;류수현;김호영;박정환
    • 한국측량학회:학술대회논문집
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    • 한국측량학회 2010년 춘계학술발표회 논문집
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    • pp.289-290
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    • 2010
  • Orthoimage, digital map, cadastral map, and areas and district were converted and overlaid so as to apply legal restrictions on boundaries set by a land object concept restriction line and to analyze boundaries formed by overlaying areas and districts and characteristics of restrictions. With the restriction lines made on the basis of orthoimage.

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Plasmid의 제한효소 지도 작성을 위한 콤퓨터 프로그램 (Rapid plasmid mapping computer program)

  • 이동훈;김영준;이승택;강현삼
    • 미생물학회지
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    • 제24권1호
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    • pp.12-17
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    • 1986
  • A new computer algorithm is described to order the restriction fragments of plasmid DNA which has been cleaved with several restriction endonucleases in single or double digestions rapidly with realistic error rates. The permutation and high weight on small fragments methods construct all logical circular map solutions. The program is written in Apple BASIC and run on an Apple II plus microcomputer with 64K memory. Several examples are presented which indicate the high efficiency of the profram in construction possible restriction map for YEp24.

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황색포도상구균에서 테트라사이클린 내성을 나타내는 플라스미드의 동정 (Characterization of Tetracycline Resistant Plasmid in Staphylococcus aureus by Restriction Enzyme Mapping)

  • 김기현;김종명;문경호
    • 약학회지
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    • 제36권3호
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    • pp.255-258
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    • 1992
  • The clinical isolate Staphylococcus aureus SA8 was resistant to tetracycline(Tc) and harboured a plasmid pKH1(24.82 kb). pKH1 was shown by curing and by transformation to specify resistance to Tc. The cleavage map of a pKH1 was determined by restricction enzyme mapping techniques. Cleavage map is given for BglII, EcoRI, HpaII, PvuII and SalI. Restriction endonuclease BamHI, BglI, BstEII, HpaI, PstI, and XhoI have no sites on this plasmid. HaeIII, XbaI, and HindIII have 5, 6, 14 sites, respectively.

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다제내성 황색포도상구균이 가지고 있는 클로람페니콜 내성 플라스미드의 동정 (Characterization of Chloramphenicol Resistant Plasmid of Multidrug-resistant Staphylococcus aureus)

  • 이대운;문경호
    • 약학회지
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    • 제37권6호
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    • pp.621-624
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    • 1993
  • The clirical isolate Staphylococcus aureus SA2 had four kinds of plasmids and was resistant to ampicillin, chloroamphenicol, clindamycin. erythromycin, gentamicin, kanamycin, methicillin, streptomycin, tetracycline and tobramycin. Transformation experiment demonstrated that 4.14kb plasmid(pKH7) encoded resistance to chloramphenicol. The cleavage map of pKH7 was determined by restriction enzyme mapping techniques. The cleavage map is given for BstEll, Hindlll, Hpall, and Xbal. The above restriction endonucleases have a single site, but nucleases BamHl, Bgll, BglII, EcoRl, EcoRV, HaeIII, Hpal, Kpnl, Pstl, PvnII, Sall, Smal, and XhoI have no site on this plasmid.

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Agrobacterium tumefaciens KU-12 균주에서 분리한 플라스미드 pTi 12의 제한효소 지도 (Restriction endonuclease mapping of the plasmid pTi12 from agrobacterium tumefaciens)

  • 이용욱;손정훈;심웅섭
    • 미생물학회지
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    • 제25권3호
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    • pp.173-179
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    • 1987
  • 한국산 A, tumefaciens의 3균주에서 분리한 Ti plasmid의 type과 분자량을 조사한 결과로 이들 모두는 octopine type 이었으며 각 plasmid의 분자량은 pTi12가 44Kb이었고, pTi14는 180Kb이었으며, pTi14는 172Kb이었다. 이중 pTi12를 Smal 및 HindIII로 가수분해한 결과 8개의 Smal digest fragment와 10개의 Hind III digest fragment를 얻었으며 Southern hybridization techniques을 이용하여 잠정적인 physical map을 작성하였다.

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Circular Permutation of the DNA Genome of Temperate Bacteriophage $\PhiFC1$ from Enterococcus faecalis KBL 703

  • Kim, Young-Woo;Jang, Se-Hwan;Hong, Bum-Shik;Lim, Wang-Jin;Kim, Chan-Wha;Sung, Ha-Chin;Chang, Hyo-Ihl
    • Journal of Microbiology and Biotechnology
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    • 제9권4호
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    • pp.457-463
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    • 1999
  • The physical map of bacteriophage $\PhiFC1$ DNA was constructed with the restriction endonucleases SalI, BamHI, EcoRI, XbaI, and AvaI. The 40.5-kb DNA restriction map is shown to be circularly permuted representing the headful packaging mechanism of the phage. The DNA restriction fragments containing the packaging initiation site(pac) was localized on the restriction map and the nucleotide sequences of the region were analyzed. Four open reading frames (ORFs), following one another with the same orientation, were found at the region. The 2nd ORF (ORF-ts) has significant amino acid sequence homologies to the previously known terminase small subunits of other bacteriophages. The putative terminase small subunit gene has a presumptive NTP-hydrolysis motif and a helix-turn-helix motif. The cleavage site for the first round of packaging was found to be located at the coding sequence of the putative terminase small subunit gene. The fourth ORF, even if partially sequenced, has a good amino acid sequence homology to the portal vertex proteins of other bacteriophages representing the evolutionarily conserved arrangements of genes near the pac site of this bacteriophage, $\PhiFC1$.

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