• 한국식품영양과학회지
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• 제38권12호
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• pp.1794-1800
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• 2009

본 연구는 학교급식의 고객만족을 위한 이벤트 마케팅의 기초자료를 제공하고자 부산지역 초-중-고등학교의 영양사 359명을 대상으로 이벤트 마케팅 실시에 대한 중요도 및 수행도, 수행효과, 애로사항 및 개선필요사항 등을 조사하였다. 조사대상자의 근무유형은 초등학교 46.2%, 중학교30.4%, 고등학교 23.4%였으며, 급식소 운영형태는 직영이 76.1%, 위탁이 23.9%로 나타났다. 학교급식 이벤트 마케팅의 중요도와 수행도에 대한 IPA 분석 결과, 중요도는 5점만점에 평균 3.39점, 수행도는 평균 2.78점으로 중요하게 생각하는데 비해 수행도가 낮은 것으로 조사되었으며 중요하게 생각하여 잘 수행하고 있는 이벤트는 계절-명절-절기-환경-학교행사-기념일 이벤트로 나타났다. 초-중-고등학 교별로 살펴본 결과, 초-고등학교의 수행도가 중학교에 비해 유의적(p<0.01)으로 높았다. 특히 중요도는 초등학교에서는 절기, 고등학교에서는 경품과 홍보 이벤트가, 수행도는 초등학교에서는 명절과 절기, 고등학교에서는 세계요리-경품-홍보 이벤트가 유의적(p<0.01)으로 높았다. 운영형태별로 살펴본 결과, 직영 급식소에 비해 위탁 급식소의 수행도가 유의적(p<0.01)으로 높았으며, 특히 중요도는 위탁에서는 학교행사와 건강 이벤트가, 수행도는 직영에서는 절기, 위탁에서는 세계요리?경품?건강 이벤트가 유의적(p<0.01)으로 높았다. 이벤트 실시 효과는 고객만족도 증가와 신뢰도(노력하고 있음을 인정) 증가 순이었다. 이벤트 수행의 애로사항으로 내부요인은 부족한 인력, 예산의 어려움, 이벤트진행 전문성 순으로 나타났으며, 외부요인은 열악한 급식시설, 낮은 식단가, 급식형태의 순으로 조사되었다. 이벤트 수행을 위한 필요개선사항은 충분한 예산과 급식인력, 급식시설개선 순으로 조사되었다. 이상의 연구 결과에서, 학교급식 영양사들은 이벤트 마케팅 실시에 대해 중요하게 생각하는데 비해 수행도가 낮은 것으로 나타났는데, 학교급식에 대한 선택권이 부족한 학생고객들에 대한 배려와 서비스 제공에 적극 노력하고 외식의 기회가 빈번해짐에 따라 입맛이 다양화되고 고급화된 학생 고객군의 급식만족도를 높이기 위한 전략의 하나로 이벤트 실시에 대해 중요하게 생각하는 것에서 나아가 좀 더 적극적으로 이벤트 실시를 계획하여 수행하는 노력이 요구된다. 특히 초등학교에 비해 이벤트 마케팅 수행도가 낮게 나타난 중학교와 고등학교에서 좀 더 적극적이고 새롭고 다양한 이벤트 실시로 감수성이 예민한 청소년들의 호기심을 자극하고 학업으로 인한 스트레스를 해소하는 즐거움을 줌으로써 학생 고객들과의 관계지행성을 높여 급식만족도를 높일 수 있도록 해야 한다. 그러나 학교급식 고객인 학생들은 인원이 많은 단체급식의 식당에서 시행되는 이벤트에 대해서 호텔이나 패밀리레스토랑과 같은 전문성을 기대하기보다는 맛있고 다양한 메뉴의 음식을 배식원들로부터 친절하게 배식받기를 기대하므로 다수의 인력을 필요로 하거나 과도한 예산을 필요로 하는 이벤트와 같은 양적인 행사진행을 계획하여 고객들이 가장 중요하게 생각하는 부분을 놓치는 실수를 범하지 말고 다양하고 신선한 메뉴를 맛있게 조리하여 진심으로 환대하는 마음으로 서비스를 제공하는 질적으로 충실한 이벤트 계획에 힘쓰는 것이 필요하다고 생각된다.

• 한국식품영양과학회지
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• 제46권2호
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• pp.251-258
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• 2017

전국의 43개 상급종합병원과 20개 상급병원의 환자 1,095명을 대상으로 환자의 영양 상담서비스에 대한 인식을 조사하고, 만족도는 서비스 품질 개념을 적용하여 분석하였다. 환자의 영양 상담서비스 인식 평균점수는 4.54/5.00점이었다. 환자의 영양 상담서비스 만족도를 요인 분석한 결과 서비스 품질 5개 영역으로 추출되었고, 각각 공감성, 반응성, 유형성, 신뢰성, 전문성으로 명명, 분류하였다. 5개 영역의 전체 평균 점수는 4.56점/5.00이었고 공감성 4.62점, 반응성 4.52점, 유형성 4.53점, 신뢰성 4.65점, 전문성 4.61점이었다. 학력에 따른 차이에서 영양 상담서비스 품질 만족도 총 평균은 대졸 이상이 중졸 이하보다 유의적(P<0.001)으로 높았다. 수도권 병원의 만족도 점수는 비수도권 병원보다 전체 평균 점수와 반응성, 공감성, 전문성, 신뢰성(P<0.001), 유형성(P<0.05)에서 유의적으로 높았다. 상담횟수에서는 반응성 영역에서 상담을 1회 받은 군이 2회보다 유의적(P<0.01)으로 높은 만족도를 보였다. 환자의 영양 상담서비스 인식과 만족도 간에 신뢰성(r=0.721), 반응성(r=0.697), 공감성(r=0.690), 전문성(r=0.678), 유형성(r=0.622)에서 양(+)의 상관관계를 보였다. 이상을 살펴볼 때 비수도권 병원에서는 임상영양사 수의 확보를 통해 환자의 영양 상담서비스 품질을 향상시키는 것이 필요하겠다. 또한, 영양 상담이 반복될수록 환자의 기대치가 높아질 수 있으므로 임상영양사는 영양 상담 내용 및 기법에 변화를 주고 다양한 자료를 활용하여 영양 상담서비스 품질을 향상시킬 수 있을 것이다. 영양 상담서비스 품질 만족도와 영양 상담 인식 간에는 높은 상관관계가 있는데 서비스 품질 만족도에서 유형성과 반응성은 다른 영역보다 상대적으로 낮은 점수를 보였으므로, 상담실 분위기를 쾌적하게 하고 다양한 교육매체를 활용하며 환자와의 여유 있는 상담시간 및 선택적 상담시간 확보 등 해당 영역에 대한 관리가 특히 필요할 것으로 여겨진다.

• Tuberculosis and Respiratory Diseases
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• 제51권2호
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• pp.122-134
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• 2001

연구배경 : 종양 세포들이 항암제에 대하여 저항을 나타내는 기전인 'apoptosis에 대한 저항'에 NF-${\kappa}B$의 활성이 중요한 역할을 하리라 생각되고 있다. 즉, NF-${\kappa}B$가 종양세포의 apoptosis를 억제하는 작용을 일으킴으로써 종양 세포의 생존에 유리하게 작용하고 있음이 제시되 고 있다. 방 법 : 본 연구에서는 비소세포폐암에 대한 항암 치료의 내성에 대하여 외부 자극으로 분해되지 않는 $I{\kappa}B{\alpha}$-SR의 삽입으로 NF-${\kappa}B$의 활성을 억제시키고 이로 인해 항암제에 대한 폐암세포주의 감수성이 증가하는지 여부를 밝혀 보고자 하였다. 결 과 : 비소세포폐암 세포주로 NCI H157, NCI H460 세포주를 이용하였고, Ad-$I{\kappa}B{\alpha}$-SR를 transduction 한 후 cisplatin을 처치한 군에서 NF-${\kappa}B$의 핵 내로의 이동이 억제되었으며 대조군과 비교시 $IC_{50}$이 2-3배 정도 유의하게 낮아짐을 관찰하였다. 또한 paclitaxel의 경우에도 Ad-$I{\kappa}B{\alpha}$-SR로 감염된 폐암세포주는 대조군과 비교시 IC50이 2배 정도 유의하게 낮아짐을 관찰하였다. 이러한 항암제에 대한 감수성의 증가의 기전으로 cisplatin의 경우는 $I{\kappa}B{\alpha}$-SR의 이입이 NF-${\kappa}B$의 활성을 억제 함으로 인한 apoptosis의 증대 때문인 것으로보인다. 결 론 : 폐암세포주에서 Ad-$I{\kappa}B{\alpha}$-SR transduction은 Clsplatin, paclitaxel에 대한 폐암세포주의 감수성을 증가시킴으로 앞으로 폐암을 치료하는데 있어 새로운 치료 방법이 될 수 있을 것으로 보인다.

• 경영정보학연구
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• 제18권1호
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• pp.57-78
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• 2016

해킹은 현대의 지식기반 사회에 심각한 문제를 야기하고 있다. 해킹으로 인한 피해 규모와 피해 금액 또한 꾸준히 증가하고 있다. 따라서 현재까지 해커 또는 해킹과 관련한 많은 연구들이 진행되어 왔다. 기존 연구에 의하면 해커들은 재미와 같은 내재적 동기에 의해 해킹 기술을 습득하며, 주로 해킹 커뮤니티에서 기술을 습득하는 것으로 알려져 왔다. 또한 최근에는 재미나 흥미 등 내재적 동기는 물론 금전적 보상 등과 같은 외재적 동기도 증가하고 있다고 한다. 그러나 현재까지의 관련 연구들은 표본 수집의 한계로 인해 정성적 연구에 치우쳐 왔다. 따라서 본 연구는 기존 연구의 한계를 극복하기 위해 해킹 커뮤니티에 소속된 멤버들을 대상으로 계획된 행동이론을 기반으로 한 정량적 연구를 진행했으며, 동기적, 사회적 그리고 환경적 요인이 해커의 학습에 미치는 영향 요인을 규명하였다. 본 연구는 보안 및 해킹 기술을 습득하기 위해 결성된 대학정보보호연합 동아리를 대상으로 2015년 5월에 약 2주 간 설문을 진행했다. 회원 전체에게 이메일을 보내 응답한 215개의 설문지를 바탕으로 연구가설을 검증하고 분석했다. 연구 결과, 해킹 기술 습득자들은 해킹 기술 습득이 사회적으로 비윤리적으로 인식되고 있다는 것을 스스로 인지하고 있었으며, 재미나 흥미와 같은 내재적 동기 및 타인의 인정을 추구하는 외재적 동기 모두 학습 태도에 영향을 주었고, 온라인 해킹 커뮤니티의 접근성과 정보 품질과 같은 환경적 특성이 해킹 기술 습득에 영향을 주는 것으로 나타났다. 본 연구는 학문적으로 해킹은 사회적으로 부정적으로 인식된다는 것을 밝혀내어 비윤리적 연구의 주관적 규범의 방향성에 대한 논의를 확장시켰고, 동기 요인들이 해킹 기술 습득 태도에 영향을 미친다는 점을 실증했다. 또한, 해킹 커뮤니티의 특성이 지각된 행동통제의 선행 요건임을 밝혀내어 계획된 행동이론의 적용 범위를 확장했다. 또한, 실무적으로 해커들을 윤리적 해커(정보보호전문가)로 만들기 위한 윤리 교육의 필요성과 해킹 방지를 위한 처벌의 강화 및 과징금 부과 등이 해커의 행동 변화에 영향을 줄 수 있음을 제시했다.

• Asia pacific journal of information systems
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• 제18권2호
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• pp.41-72
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• 2008

The purpose of this study is the investigation of the effects of user participation, network effect, social influence, and usefulness on stickiness and continued use on Internet communities. In this research, stickiness refers to repeat visit and visit duration to an Internet community. Continued use means the willingness to continue to use an Internet community in the future. Internet community-based companies can earn money through selling the digital contents such as game, music, and avatar, advertizing on internet site, or offering an affiliate marketing. For such money making, stickiness and continued use of Internet users is much more important than the number of Internet users. We tried to answer following three questions. Fist, what is the effects of user participation on stickiness and continued use on Internet communities? Second, by what is user participation formed? Third, are network effect, social influence, and usefulness that was significant at prior research about technology acceptance model(TAM) still significant on internet communities? In this study, user participation, network effect, social influence, and usefulness are independent variables, stickiness is mediating variable, and continued use is dependent variable. Among independent variables, we are focused on user participation. User participation means that Internet user participates in the development of Internet community site (called mini-hompy or blog in Korea). User participation was studied from 1970 to 1997 at the research area of information system. But since 1997 when Internet started to spread to the public, user participation has hardly been studied. Given the importance of user participation at the success of Internet-based companies, it is very meaningful to study the research topic of user participation. To test the proposed model, we used a data set generated from the survey. The survey instrument was designed on the basis of a comprehensive literature review and interviews of experts, and was refined through several rounds of pretests, revisions, and pilot tests. The respondents of survey were the undergraduates and the graduate students who mainly used Internet communities. Data analysis was conducted using 217 respondents(response rate, 97.7 percent). We used structural equation modeling(SEM) implemented in partial least square(PLS). We chose PLS for two reason. First, our model has formative constructs. PLS uses components-based algorithm and can estimated formative constructs. Second, PLS is more appropriate when the research model is in an early stage of development. A review of the literature suggests that empirical tests of user participation is still sparse. The test of model was executed in the order of three research questions. First user participation had the direct effects on stickiness(${\beta}$=0.150, p<0.01) and continued use (${\beta}$=0.119, p<0.05). And user participation, as a partial mediation model, had a indirect effect on continued use mediated through stickiness (${\beta}$=0.007, p<0.05). Second, optional participation and prosuming participation significantly formed user participation. Optional participation, with a path magnitude as high as 0.986 (p<0.001), is a key determinant for the strength of user participation. Third, Network effect (${\beta}$=0.236, p<0.001). social influence (${\beta}$=0.135, p<0.05), and usefulness (${\beta}$=0.343, p<0.001) had directly significant impacts on stickiness. But network effect and social influence, as a full mediation model, had both indirectly significant impacts on continued use mediated through stickiness (${\beta}$=0.11, p<0.001, and ${\beta}$=0.063, p<0.05, respectively). Compared with this result, usefulness, as a partial mediation model, had a direct impact on continued use and a indirect impact on continued use mediated through stickiness. This study has three contributions. First this is the first empirical study showing that user participation is the significant driver of continued use. The researchers of information system have hardly studies user participation since late 1990s. And the researchers of marketing have studied a few lately. Second, this study enhanced the understanding of user participation. Up to recently, user participation has been studied from the bipolar viewpoint of participation v.s non-participation. Also, even the study on participation has been studied from the point of limited optional participation. But, this study proved the existence of prosuming participation to design and produce products or services, besides optional participation. And this study empirically proved that optional participation and prosuming participation were the key determinant for user participation. Third, our study compliments traditional studies of TAM. According prior literature about of TAM, the constructs of network effect, social influence, and usefulness had effects on the technology adoption. This study proved that these constructs still are significant on Internet communities.

• Asia pacific journal of information systems
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• 제21권3호
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• pp.71-96
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• 2011

Within the traditional medical delivery system, patients residing in medically vulnerable areas, those with body movement difficulties, and nursing facility residents have had limited access to good healthcare services. However, Information and Communication Technology (ICT) provides us with a convenient and useful means of overcoming distance and time constraints. ICT is integrated with biomedical science and technology in a way that offers a new high-quality medical service. As a result, rapid technological advancement is expected to play a pivotal role bringing about innovation in a wide range of medical service areas, such as medical management, testing, diagnosis, and treatment; offering new and improved healthcare services; and effecting dramatic changes in current medical services. The increase in aging population and chronic diseases has caused an increase in medical expenses. In response to the increasing demand for efficient healthcare services, a telehealth service based on ICT is being emphasized on a global level. Telehealth services have been implemented especially in pilot projects and system development and technological research. With the service about to be implemented in earnest, it is necessary to study its overall acceptance by consumers, which is expected to contribute to the development and activation of a variety of services. In this sense, the study aims at positively examining the structural relationship among the acceptance factors for telehealth services based on the Technology Acceptance Model (TAM). Data were collected by showing audiovisual material on telehealth services to online panels and requesting them to respond to a structured questionnaire sheet, which is known as the information acceleration method. Among the 1,165 adult respondents, 608 valid samples were finally chosen, while the remaining were excluded because of incomplete answers or allotted time overrun. In order to test the reliability and validity of the assessment scale items, we carried out reliability and factor analyses, and in order to explore the causal relation among potential variables, we conducted a structural equation modeling analysis using AMOS 7.0 and SPSS 17.0. The research outcomes are as follows. First, service quality, innovativeness of medical technology, and social influence were shown to affect perceived ease of use and perceived usefulness of the telehealth service, which was statistically significant, and the two factors had a positive impact on willingness to accept the telehealth service. In addition, social influence had a direct, significant effect on intention to use, which is paralleled by the TAM used in previous research on technology acceptance. This shows that the research model proposed in the study effectively explains the acceptance of the telehealth service. Second, the research model reveals that information privacy concerns had a insignificant impact on perceived ease of use of the telehealth service. From this, it can be gathered that the concerns over information protection and security are reduced further due to advancements in information technology compared to the initial period in the information technology industry, and thus the improvement in quality of medical services appeared to ensure that information privacy concerns did not act as a prohibiting factor in the acceptance of the telehealth service. Thus, if other factors have an enormous impact on ease of use and usefulness, concerns over these results in the initial period of technology acceptance may become irrelevant. However, it is clear that users' information privacy concerns, as other studies have revealed, is a major factor affecting technology acceptance. Thus, caution must be exercised while interpreting the result, and further study is required on the issue. Numerous information technologies with outstanding performance and innovativeness often attract few consumers. A revised bill for those urgently in need of telehealth services is about to be approved in the national assembly. As telemedicine is implemented between doctors and patients, a wide range of systems that will improve the quality of healthcare services will be designed. In this sense, the study on the consumer acceptance of telehealth services is meaningful and offers strong academic evidence. Based on the implications, it can be expected to contribute to the activation of telehealth services. Further study is needed to assess the acceptance factors for telehealth services, such as motivation to remain healthy, health care involvement, knowledge on health, and control of health-related behavior, in order to develop unique services according to the categorization of customers based on health factors. In addition, further study may focus on various theoretical cognitive behavior models other than the TAM, such as the health belief model.

• Asia pacific journal of information systems
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• 제19권3호
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• pp.51-67
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• 2009

Developing an agile recommender system for nomadic users has been regarded as a promising application in mobile and ubiquitous settings. To increase the quality of personalized recommendation in terms of accuracy and elapsed time, estimating future context of the user in a correct way is highly crucial. Traditionally, time series analysis and Makovian process have been adopted for such forecasting. However, these methods are not adequate in predicting context data, only because most of context data are represented as nominal scale. To resolve these limitations, the alignment-prediction algorithm has been suggested for context prediction, especially for future context from the low-level context. Recently, an ontological approach has been proposed for guided context prediction without context history. However, due to variety of context information, acquiring sufficient context prediction knowledge a priori is not easy in most of service domains. Hence, the purpose of this paper is to propose a novel context prediction methodology, which does not require a priori knowledge, and to increase accuracy and decrease elapsed time for service response. To do so, we have newly developed pattern-based context prediction approach. First of ail, a set of individual rules is derived from each context attribute using context history. Then a pattern consisted of results from reasoning individual rules, is developed for pattern learning. If at least one context property matches, say R, then regard the pattern as right. If the pattern is new, add right pattern, set the value of mismatched properties = 0, freq = 1 and w(R, 1). Otherwise, increase the frequency of the matched right pattern by 1 and then set w(R,freq). After finishing training, if the frequency is greater than a threshold value, then save the right pattern in knowledge base. On the other hand, if at least one context property matches, say W, then regard the pattern as wrong. If the pattern is new, modify the result into wrong answer, add right pattern, and set frequency to 1 and w(W, 1). Or, increase the matched wrong pattern's frequency by 1 and then set w(W, freq). After finishing training, if the frequency value is greater than a threshold level, then save the wrong pattern on the knowledge basis. Then, context prediction is performed with combinatorial rules as follows: first, identify current context. Second, find matched patterns from right patterns. If there is no pattern matched, then find a matching pattern from wrong patterns. If a matching pattern is not found, then choose one context property whose predictability is higher than that of any other properties. To show the feasibility of the methodology proposed in this paper, we collected actual context history from the travelers who had visited the largest amusement park in Korea. As a result, 400 context records were collected in 2009. Then we randomly selected 70% of the records as training data. The rest were selected as testing data. To examine the performance of the methodology, prediction accuracy and elapsed time were chosen as measures. We compared the performance with case-based reasoning and voting methods. Through a simulation test, we conclude that our methodology is clearly better than CBR and voting methods in terms of accuracy and elapsed time. This shows that the methodology is relatively valid and scalable. As a second round of the experiment, we compared a full model to a partial model. A full model indicates that right and wrong patterns are used for reasoning the future context. On the other hand, a partial model means that the reasoning is performed only with right patterns, which is generally adopted in the legacy alignment-prediction method. It turned out that a full model is better than a partial model in terms of the accuracy while partial model is better when considering elapsed time. As a last experiment, we took into our consideration potential privacy problems that might arise among the users. To mediate such concern, we excluded such context properties as date of tour and user profiles such as gender and age. The outcome shows that preserving privacy is endurable. Contributions of this paper are as follows: First, academically, we have improved sequential matching methods to predict accuracy and service time by considering individual rules of each context property and learning from wrong patterns. Second, the proposed method is found to be quite effective for privacy preserving applications, which are frequently required by B2C context-aware services; the privacy preserving system applying the proposed method successfully can also decrease elapsed time. Hence, the method is very practical in establishing privacy preserving context-aware services. Our future research issues taking into account some limitations in this paper can be summarized as follows. First, user acceptance or usability will be tested with actual users in order to prove the value of the prototype system. Second, we will apply the proposed method to more general application domains as this paper focused on tourism in amusement park.

• 종양간호연구
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• 제3권2호
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• pp.133-144
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• 2003

This study is carrying out a before and after experiment design for the non-equal comparative group to identify the effects of the breast self-examination education on breast self-examination participant education compliance and health promotion in women. The subject of this study was 58 women residing in Chungju. Their age ranged from 20 to 40. These women were not pregnant or did not breast feed, as well as they did not have any breast disease, at the time of survey. They were available for the response to the questionnaires, and understood the purpose of this study. They also agreed to participate in the study, and responded to the 3rd time questionnaires to the end. Thus, brochures and lectures were provided side by side to a group of 19 of those women, and only brochures were provided to another group of 39 of those women. With regard to education, a brochure and a program using a breast model were applied. Then, the frequency of the breast self examination compliance, breast self examination capability and the relations between the breast self examination and activities to promote health were measured, before the education, after 4 weeks of education and after 12 weeks of education. Before education and after 4 weeks of education. I collected the questionnaires myself visiting them, and after 12 weeks of education, the questionnaires were collected by mail. In relation to the study tool, the breast self examination activity was measured by two measuring tools: breast self examination activity frequency and breast self examination compliance capability. As for the frequency, the number of self examination for the period of 3 months, before the questionnaire survey, was measured in the form of self report. In relation to the tool to measure the breast self examination capability, the BSEPRI tool, which was developed by Wood in 1994, was used. Here, as the score was higher, the capability was indicated to be higher. The translated and revised version of Health Promoting Lifestyle Profile II (Walker, Sechrist & Pender. 2002) was used as a tool for health promotion. As the measured score was higher, the health promoting activity was indicated to be higher. The results of this study were as follows: 1. As a result of checking the breast self examination compliance frequency between the experiment group that received the breast self examination participant education and the comparative group that did not receive the education, there was a significant difference in interaction between groups by time, as time passed by. 2. As a result of checking the breast self examination compliance capability between the experiment group and the comparative group, there was a significant difference in interaction between groups by time, as time passed by. 3. As a result of carrying out a repetitive measurement analysis, between the experiment group that received the breast self examination education and the comparative group that did not receive the education, which was carried out to validate the hypothesis that the former would have higher health promoting activities than the latter, there was no significant difference after the breast self examination education was conducted.

• 생명과학회지
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• 제19권9호
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• pp.1328-1332
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• 2009

고래회충유충증은 해산어류에 기생하는 고래회충과(family Anisakidae)에 속하는 선충류 유충에 의한 질병으로 유충의 직접적인 위장관내 침입으로 인한 병변과 더불어 유충의 분비 배설물에 의한 알레르기 질환도 유발될 수 있다. 고래회충유충증은 A. simplex를 비롯하여 Contracaecum, Pseudoterranova, Hysterothylacium 등의 유충에 의해 야기될 수 있으나 이들에 대한 형태학적 감별 진단은 유충의 형태적 유사성으로 인하여 매우 어려운 경우가 많다. 이러한 형태학적 진단의 어려움을 극복하고 분자생물학적 감별진단 방법을 확립하기 위하여 A. simplex, Contracaecum type A. type C' 및 Goezia 유충을 숭어, 도다리, 고등어, 아나고, 참돔 등 5종의 어류에서 분리하였다. 각각의 유충으로부터 분리한 18S rDNA를 PCR로 증폭한 후 Taq 1, Hinf I, Hha I, Alu 1, Dde I, Hae III, Sau 96I, Sau 3AI 등 8종의 제한효소를 사용하여 PCR-RFLP를 시행하였다. PCR product의 크기는 약 2.0 Kb였으며 Hinf l, Alu 1, Hha I, Dde 1 및 Hae III로 A. simplex와 Contracaecum type C'을 구분할 수 있었다. 그러나 Contracaecum type A의 경우에는 Taq I, Hinf I, Alu I 및 Dde I의 경우에는 2가지 패턴으로 나타났으며 이들 가운데 일부는 A. simplex, Contracaecum type C', 및 Goezia와 동일한 분석 패턴을 보이기도 하였다. Goezia는 사용한 8개의 제한 효소 모두에서 A. simplex 및 Contracaecum type A 및 type C'과 각기 다른 양상을 보였다. 이러한 결과로 18S rDNA PCR-RFLP 방법은 A. simplex와 Contracaecum type C'의 감별 진단에 유용한 것으로 밝혀졌으며, Contracaecum type A의 분류에는 제한적으로 사용되어야 함은 물론 형태학적 분류 기준에 대한 재검토가 뒤따라야 할 것으로 사료되었다.

• 생명과학회지
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• 제30권10호
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• pp.905-911
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• 2020

본 연구에서는 Keyhole limpet hemocyanin (KLH)에 대한 체액성 및 세포성 면역반응 유도에 대한 PAMAM dendrimer G4 (PAMAM)의 증강 효과를 조사하였다. PAMAM을 KLH와 2주 간격으로 2회 피하주사로 면역한 후, KLH에 대한 특이항체를 측정한 결과, KLH+PAMAM 면역 그룹은 KLH만을 단독으로 면역한 그룹에 약 30배이상 높은 유의한 항체가(IgG+IgA+IgM) 상승을 나타냈다. ELISA 분석에 의해 KLH 특이적인 면역글로부린의 isotype을 측정한 결과, PAMAM를 혼합하여 면역함으로서 IgG1, IgG2a, IgG2b, IgG3 및 IgM 항체의 역가가 유의하게 증가하는 것으로 확인되었다. 또한 면역 개시 7주째에 면역동물에 KLH 항원을 피하주사하고 swelling reaction을 통해 세포성 면역반응인 지연형 과민반응(DTH)을 측정한 결과, KLH+PAMAM으로 면역한 그룹에서 KLH만을 단독으로 면역한 그룹에 비해 높은 DTH 유도활성이 관찰되었다. 한편 면역동물의 비장세포를 취하여 in vitro에서 KLH로 재자극한 후 림프구 증식반응과 사이토카인 유도활성을 측정한 결과, PAMAM을 혼합하여 면역한 그룹에서 KLH 단독 면역 그룹에 비해 림프구의 증식반응에 유의하게 증가하였으며, Th1 type (IFN-γ)과 Th2 type (IL-4) 사이토카인의 생성도 모두 상승하는 것으로 확인되었다. 이상의 결과로부터 PAMAM dendrimer는 함께 투여된 항원물질에 의해 유도되는 세포성 면역과 체액성 면역을 상승시키는 활성이 있는 것으로 확인되었으며, 이는 PAMAM dendrimer가 면역 adjuvant로서 응용 가능한 소재임을 입증하는 것이다.