• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.11 no.6
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• pp.175-180
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• 2001

Dynamic response localization of simple mistuned periodic structures is presented in this paper Mistuning in periodic structures can cause forced responses that are much larger than those of perfectly tuned structures. So mistuning results in the critical impact on high cycle fatigue of structures. Thus, it is of great importance to predict the mistuned forced response in an efficient way. In this paper, forced responses of coupled pendulum systems are investigated to identify the localization effect of periodic structures. The effects of mistuning and damping on the maximum forced response are examined. It is found that certain conditions of mistuning and coupling can cause strong localization and the localization becomes significant under weak damping. It is also found that the maximum forced response increases as the number of Periodic structures increases.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.14 no.6
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• pp.478-485
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• 2004

Vibration localization characteristics of mistuned repeated structures are investigated. The mistuning often creates significant response discrepancies among subcomponents of a repeated structure. As the result of the discrepancies, critical fatigue often occurs in a repeated structure. Therefore, it is of great importance to predict the vibration response of the mistuned repeated structures accurately. In this paper, a simplified model is employed and dimensionless parameters that influence the localization characteristics are identified. Through parameter study, two localization phenomena are investigated and compared.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2003.11a
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• pp.704-709
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• 2003

Vibration localization characteristics of repeated mistuned structures are investigated by two ways. The mistuning often creates significant response discrepancies among subcomponents of the repeated structures. As a result of the discrepancies, critical fatigue problems often occur in repeated structures. Therefore, it is of great importance to predict the vibration response of the mistuned repeated structures accurately. In this paper, a simplified model is employed and dimensionless parameters that influence the localization characteristics are first identified. Through the parameter study, the two localization phenomena are investigated and compared.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2000.06a
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• pp.543-548
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• 2000

Vibration localization of a periodic structure with mistuning is presented in this paper. Mistuning in periodic structures can lead to an increase of the forced response which is much larger than those of perfectly tuned assembly. Thus, mistuning has a critical impact on high cycle fatigue in structures, and it is of great importance to predict the mistuned forced response in efficient manner. In this paper, forced response of a coupled pendulum is investigated to identify localization effects of periodic structures. The effects of mistuning and damping on the maximum forced response are examined. It is seen that in certain condition of mistuning and coupling, strong localization occurs and this can be significant under weak damping.

• Transactions of the Korean Society for Noise and Vibration Engineering
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• v.13 no.12
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• pp.911-917
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• 2003

Vibration localization characteristics of open loop repeated mistuned structures are investigated. The mistuning often creates significant response discrepancies among subcomponents of the repeated structures. As a result of the discrepancies, critical fatigue problems often occur in repeated structures. Therefore. it is of great importance to predict the vibration response of the mistuned repeated structures accurately in this Paper, a simplified model for an open-loop repeated structure is introduced and dimensionless parameters that influence the localization characteristics are identified. The effects of the parameters on the localization characteristics are investigated through numerical study.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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• 2003.05a
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• pp.489-494
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• 2003

Vibration localization characteristics of open loop repeated structures with mistuning are investigated in this paper. Mistuning of a periodic structure often creates significant non-uniformity in vibration responses. As a result of the localization, critical fatigue problems often occur in repeated structures. Therefore, it is of great importance to predict the vibration response of the mistuned repeated structures accurately. In this paper, a simplified model for the open-loop repeated structure is introduced and dimensionless parameters which influence the localization characteristics are identified. The effects of the parameters on the localization characteristics are investigated through numerical study.

• Journal of Mechanical Science and Technology
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• v.19 no.7
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• pp.1405-1413
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• 2005

Malfunctions or critical fatigue problems often occur in mistuned periodic structural systems since their vibration responses may become much larger than those of perfectly tuned periodic systems. These are called vibration localization phenomena and it is of great importance to accurately predict the localization phenomena for safe and reliable designs of the periodic structural systems. In this study, a simple discrete system which represents periodic structural systems is employed to analyze the vibration localization phenomena. The statistical effects of mistuning, stiffness coupling, and damping on the vibration localization phenomena are investigated through Monte Carlo simulation. It is found that the probability of vibration localization was significantly influenced by the statistical properties except the standard deviation of coupling stiffness.

• Bulletin of the Korean Chemical Society
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• v.30 no.9
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• pp.1981-1984
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• 2009

Subcellular localization of protein kinase often plays an important role in determining its activity and specificity. Protein kinase C (PKC), a family of multi-gene protein kinases has long been known to be translocated to the particular cellular compartments in response to DAG or its analog phorbol esters. We used C-terminal green fluorescent protein (GFP) fusion proteins of PKC isoforms to visualize the subcellular distribution of individual PKC isoforms. Intracellular localization of PKC-GFP proteins was monitored by fluorescence microscopy after transient transfection of PKC-GFP expression vectors in the HeLa cells. In unstimulated HeLa cells, all PKC isoforms were found to be distributed throughout the cytoplasm with a few exceptions. PKC$\theta$ was mostly localized to the Golgi, and PKC$\gamma$, PKC$\delta$ and PKC$\eta$ showed cytoplasmic distribution with Golgi localization. DAG analog TPA induced translocation of PKC-GFP to the plasma membrane. PKC$\alpha$, PKC$\eta$ and PKC$\theta$ were also localized to the Golgi in response to TPA. Only PKC$\delta$ was found to be associated with the nuclear membrane after transient TPA treatment. These results suggest that specific PKC isoforms are translocated to different intracellular sites and exhibit distinct biological effects.

• The Journal of the Acoustical Society of Korea
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• v.36 no.2
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• pp.100-107
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• 2017

The focused MVDR (Minimum Variance Distortionless Response) can be applied for source localization in near field. However, if the number of sensors are increased, it requires a large amount of calculation to obtain the inverse of the covariance matrix. In this paper we propose a focused MVDR method using that beam space is formed from output of far field beamformer at the subarray. The performances of the proposed method was evaluated by simulation. As a result of simulation, the proposed method has the higher spatial resolution performance then the conventional delay-and-sum beamformer.

• Molecules and Cells
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• v.37 no.4
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• pp.307-313
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• 2014

Cell migration requires a defined cell polarity which is formed by diverse cytoskeletal components differentially localized to the poles of cells to extracellular signals. Rap-GAP3 transiently and rapidly translocates to the cell cortex in response to chemoattractant stimulation and localizes to the leading edge of migrating cells. Here, we examined localization of truncated RapGAP3 proteins and found that the I/LWEQ domain in the central region of RapGAP3 was sufficient for posterior localization in migrating cells, as opposed to leading-edge localization of full-length Rap-GAP3. All truncated proteins accumulated at the leading edge of migrating cells exhibited clear translocation to the cell cortex in response to stimulation, whereas proteins localized to the posterior in migrating cells displayed no translocation to the cortex. The I/LWEQ domain appears to passively accumulate at the posterior region in migrating cells due to exclusion from the extended front region in response to chemoattractant stimulation rather than actively being localized to the back of cells. Our results suggest that posterior localization of the I/LWEQ domain of RapGAP3 is likely related to F-actin, which has probably different properties compared to newly formed F-actin at the leading edge of migrating cells, at the lateral and posterior regions of the cell.