• The Plant Pathology Journal
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• 제37권6호
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• pp.512-520
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• 2021

In this study, we report genetic characterization of Orobanche cumana, the causal agent of sunflower wilting in Serbia. The genetic diversity of this parasitic plant in Serbia was not studied before. Random amplified polymorphic DNA (RAPD) markers and partial rbcL gene sequences analysis were used to characterize the O. cumana populations at the molecular level. While phylogenetic analyses of RAPD-PCR amplicons were performed using unweighted pair-group Method analyses, rbcL gene sequences were analyzed using neigbor joining method and minimum spanning tree. Molecular analyses of RAPD-PCR analysis revealed high genetic diversity of O. cumana populations which indicated high adaptive potential of this parasitic weed in Serbia. Further analyses of rbcL gene using minimum spanning tree revealed clear differences among diverse sections of Orobanche genus. Although this molecular marker lacked the resolution to display intrapopulation diversity it could be a useful tool for understanding the evolution of this parasitic plant. Our results suggested that O. cumana has great genetic potential which can lead to differentiation of more virulent races which is important for determining crop breeding strategies for their control.

• Investigative Magnetic Resonance Imaging
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• 제25권4호
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• pp.252-265
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• 2021

Cardiac magnetic resonance imaging (MRI) serves as a clinical gold-standard non-invasive imaging technique for the assessment of global and regional cardiac function. Conventional cardiac MRI is limited by the long acquisition time, the need for ECG gating and/or long breathhold, and insufficient spatiotemporal resolution. Real-time cardiac cine MRI refers to high spatiotemporal cardiac imaging using data acquired continuously without synchronization or binning, and therefore of potential interest in overcoming the limitations of conventional cardiac MRI. Novel acquisition and reconstruction techniques must be employed to facilitate real-time cardiac MRI. The goal of this study is to discuss methods that have been developed for real-time cardiac MRI. In particular, we classified existing techniques into two categories based on the use of non-iterative and iterative reconstruction. In addition, we present several research trends in this direction, including deep learning-based image reconstruction and other advanced real-time cardiac MRI strategies that reconstruct images acquired from real-time free-breathing techniques.

• Safety and Health at Work
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• 제13권4호
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• pp.448-455
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• 2022

Background: While the organizational factors that account for firefighters' burnout have been extensively explored, the individual factors related to how they regulate interpersonal conflicts and emotions remain to be investigated. Previous research has demonstrated the association between emotional intelligence and conflict management styles and burnout, but no study has looked at the interrelationships among these factors in high-risk sectors. The present exploratory study aimed to fill this research gap by investigating the relationships between conflict management style, emotional intelligence and burnout in a sample of firefighters. Methods: A cross-sectional study was conducted with 240 French firefighters. Measures comprised validated scales of conflict management styles, emotional intelligence and burnout. Results: Results showed that the integrating conflict style reduced burnout. They also revealed the effects of emotion regulation on burnout, whereby the awareness and management of one's own emotions reduced burnout. Moreover, awareness of one's own emotions moderated the relationship between integrating conflict resolution style and burnout, whereby the effect of integrating style on reduced burnout was higher when awareness of one's own emotions was high. Conclusion: These results reveal that strategies used by firefighters to regulate their emotions in order to meet the emotional demands specific to their job are important for reducing the emotional exhaustion component of burnout. Training programs for conflict and emotion management are needed to preserve the mental health of firefighters and ensure the safety of interventions.

• Journal of Radiation Protection and Research
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• 제47권4호
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• pp.226-236
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• 2022

Background: An effective communication strategy for reducing conflicts in South Korea has been designed through the analysis of public perception and communication variables on nuclear power under the conditions of rapidly changing nuclear power policies. Materials and Methods: This study conducted both qualitative research through group discussions based on social psychology and quantitative research through surveys. Results and Discussion: Nuclear power plant (NPP) area residents in favor of nuclear power indicated higher levels of communication, safety perception, and contribution than those against it. NPP area residents trusted the civilian expert groups (18.3%) and local government (17.3%) the most, while metropolitan city residents trusted the Nuclear Safety and Security Commission and the Korea Institute of Nuclear Safety (20.7%) the most. In determining nuclear power policy, both the NPP area residents (18.1%) and metropolitan city residents (17.1%) prioritized safety, health, and the environment. While metropolitan city residents thought that energy security and economic growth (16.4%) were important, NPP area residents thought the current issue of spent fuel rods (14.1%) to be important. Conclusion: It is necessary for the nuclear power industry to have and actively implement communication and conflict resolution strategies based on the patterns obtained in the study results.

• 한국표면공학회지
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• 제57권3호
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• pp.179-191
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• 2024

This study focuses on improving the photocatalytic degradation efficiency by synthesizing a TiO₂/WO₃ composite. Given the environmental significance of photocatalysis and the limitations posed by TiO₂'s large bandgap and high electron recombination rate, we explored doping, surface modification, and synthesis strategies. The composite was created using a ball mill process and heat treatment, analyzed with field emission scanning electron microscope, high resolution X-ray diffraction, Raman microscope, and UV-Vis/NIR spectrometer to examine its morphology, composition and absorbance. We found that incorporating WO₃ into the TiO₂ lattice forms a W_x-Ti_1-x-O₂ solution, with optimal WO₃ content reducing the band gap and enhancing sterilization efficiency by inhibiting the anatasese to rutile transition. This contributes to the field by offering a way to overcome TiO₂'s limitations and improve photocatalytic performance.

• The Korean Journal of Physiology and Pharmacology
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• 제28권5호
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• pp.403-411
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• 2024

The global spread of flaviviruses has triggered major outbreaks worldwide, significantly impacting public health, society, and economies. This has intensified research efforts to understand how flaviviruses interact with their hosts and manipulate the immune system, underscoring the need for advanced research tools. RNA-sequencing (RNA-seq) technologies have revolutionized our understanding of flavivirus infections by offering transcriptome analysis to dissect the intricate dynamics of virus-host interactions. Bulk RNA-seq provides a macroscopic overview of gene expression changes in virus-infected cells, offering insights into infection mechanisms and host responses at the molecular level. Single-cell RNA sequencing (scRNA-seq) provides unprecedented resolution by analyzing individual infected cells, revealing remarkable cellular heterogeneity within the host response. A particularly innovative advancement, virus-inclusive single-cell RNA sequencing (viscRNA-seq), addresses the challenges posed by non-polyadenylated flavivirus genomes, unveiling intricate details of virus-host interactions. In this review, we discuss the contributions of bulk RNA-seq, scRNA-seq, and viscRNA-seq to the field, exploring their implications in cell line experiments and studies on patients infected with various flavivirus species. Comprehensive transcriptome analyses from RNA-seq technologies are pivotal in accelerating the development of effective diagnostics and therapeutics, paving the way for innovative treatments and enhancing our preparedness for future outbreaks.

• 대한간호학회지
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• 제27권1호
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• pp.169-177
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• 1997

This study aims at empirically clarifying the relationship between power and the interpersonal conflict, including nurses' understanding of their relative power, the causes of interpersonal conflicts with the nurses, and strategies to resolve conflicts, in order to understand how nurses' relative power affect their conflicts. For the empirical survey, the population was defined as all the nurses working at a medical organization in Seoul, Korea. 1083 nurses were selected as the sample for the questionnaire survey and statistical analyses. For the sampling, 32 medical organizations were selected by a stratified random method and sub-samples were arbitrarily drawn from each organization to obtain the final sample of 1083 nurses who responded to the questionnaire designed by the reseacher. According to the result of the study, most nurses experience conflict more than once a month, and 70.4% of the respondents answered that interpersonal conflicts were directly or indirectly caused by power relations. which indicates that they perceive power relations as the main cause of interpersonal conflicts. Nurses experienced the most conflicts with interns and residents(29.7%), then patients and their families(24.3%), higher-positioned nurses(12.3%), nurse colleagues(7.7%), lower-positioned nurses(6.5%), and staff doctors(5.1%). If we classify these into three groups. the frequency of the conflicts, from most frequent to least. is in the order of doctors. nurses, and patients. In terms of relative power, nurses perceive that they have greater power than patients and their families, lower-positioned nurses, and nurse colleagues. In contrast, nurses perceive that they have less power than interns and residents, higher-positioned nurses. and staff doctors. Among these groups. nurses perceive that they have the most power over patients and the least over staff doctors. These results indicate that nurses tend to experience more conflicts with members of groups that are stronger than themselves in terms of relative power, Nurses use positive strategies such as the compromise strategy(32.3%) or the collaboration strategy (20.3%) to manage conflicts, more than other strategies. However, they use avoidance or competition strategy more at the earlier stage, compromise strategy more in the mid stage, and collaboration strategy more at the later stage of the interpersonal conflict. In relation to power, nurses use the collaboration strategy or the compromise strategy more when their perceived power is greater than or equal to their counterpart's, and they use the avoidance strategy or the accommodation strategy if their power is less. In terms of source of power, nurses' perceived relative power is greater in the order of referent power. expert power, reward power, legitimate power. and coercive power. where referent power is perceived as having the greatest power and coercive power is least. Most nurses(69.3%) used their power to resolve a conflict. with positive outcomes. Expert power was used most frequently. Overall. this study strongly indicates that the enhancement of power of nurses to have equal power relations with doctors would heighten the success of conflict resolution, since power is the main cause of conflicts. Specifcally. nurses experience most conflicts with doctors against whom they perceive the greatest gap in power. and the choice of a conflict management strategy depend upon their power relations.

• 한국미생물생명공학회:학술대회논문집
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• 한국미생물생명공학회 2004년도 Annual Meeting BioExibition International Symposium
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• pp.60-61
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• 2004

Metabolic engineering is now a well established discipline, used extensively to determine and execute rational strategies of strain development to improve the performance of micro-organisms employed in industrial fermentations. The basic principle of this approach is that performance of the microbial catalyst should be adequately characterised metabolically so as to clearlyidentify the metabolic network constraints, thereby identifying the most probable targets for genetic engineering and the extent to which improvements can be realistically achieved. In order to harness correctly this potential, it is clear that the physiological analysis of each strain studied needs to be undertaken under conditions as close as possible to the physico-chemical environment in which the strain evolves within the full-scale process. Furthermore, this analysis needs to be undertaken throughoutthe entire fermentation so as to take into account the changing environment in an essentially dynamic situation in which metabolic stress is accentuated by the microbial activity itself, leading to increasingly important stress response at a metabolic level. All too often these industrial fermentation constraints are overlooked, leading to identification of targets whose validity within the industrial context is at best limited. Thus the conceptual error is linked to experimental design rather than inadequate methodology. New tools are becoming available which open up new possibilities in metabolic engineering and the characterisation of complex metabolic networks. Traditionally metabolic analysis was targeted towards pre-identified genes and their corresponding enzymatic activities within pre-selected metabolic pathways. Those pathways not included at the onset were intrinsically removed from the network giving a fundamentally localised vision of pathway functionality. New tools from genome research extend this reductive approach so as to include the global characteristics of a given biological model which can now be seen as an integrated functional unit rather than a specific sub-group of biochemical reactions, thereby facilitating the resolution of complexnetworks whose exact composition cannot be estimated at the onset. This global overview of whole cell physiology enables new targets to be identified which would classically not have been suspected previously. Of course, as with all powerful analytical tools, post-genomic technology must be used carefully so as to avoid expensive errors. This is not always the case and the data obtained need to be examined carefully to avoid embarking on the study of artefacts due to poor understanding of cell biology. These basic developments and the underlying concepts will be illustrated with examples from the author's laboratory concerning the industrial production of commodity chemicals using a number of industrially important bacteria. The different levels of possibleinvestigation and the extent to which the data can be extrapolated will be highlighted together with the extent to which realistic yield targets can be attained. Genetic engineering strategies and the performance of the resulting strains will be examined within the context of the prevailing experimental conditions encountered in the industrial fermentor. Examples used will include the production of amino acids, vitamins and polysaccharides. In each case metabolic constraints can be identified and the extent to which performance can be enhanced predicted

• 한국지역지리학회지
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• 제9권3호
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• pp.297-313
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• 2003

그 동안 미군기지로 인해 지역사회에서 발생한 미시적 문제는 거시-지정학적 관점에서 국가안보와 동북아 평화를 이유로 관심을 끌지 못했다. 그러나 1990년대 이후 통치체제가 민주화되고 시민사회의 정치적 담론이 활성화되면서, 이 문제들은 지역사회 나아가 전국적으로 주요한 이슈가 되고 있다. 지역사회에서 발생하는 미군 문제들에 접근하기 위하여 미시 지정학적 관점이 주요한 의미를 가지게 되었다. 이러한 점에서, 본 논문은 미군 주둔이 지역사회에 미치는 부정적 영향으로 사회적 범죄와 환경오염 문제에 초점을 두고 이로 인해 발생하는 지역사회의 문제와 이에 대한 대책을 미시-지정학적 관점에서 분석하고자 한다. 우선 최근 정치지리학에서 지역사회에서 유발되는 문제와 생활정치 등에 초점을 두는 미시-비판적 접근의 중요성을 강조하고자 한다. 그리고 이에 기초하여 미관 기지로 인해 지역사회에서 발생하는 주요 문제 유형으로서 범죄와 환경오염의 실태를 살펴보면, 대구시 남구의 미군기지 주변 주민들에 대한 설문조사 자료를 통해 주민의적을 분석하였으며, 끝으로 미군 주둔으로 인해 발생하는 이러한 문제들에 대한 주요 대책들을 제시하고자 한다.

• 대한원격탐사학회지
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• 제37권6_1호
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• pp.1773-1784
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• 2021

해상에서 발생하는 유출유 사고는 피해 최소화를 위해서 신속한 현황 정보 수집이 필수적이며, 인공위성은 해상에 유출된 기름을 탐지하는데 매우 유용한 도구이다. 최근에 활용 가능한 인공위성 수가 급속하게 증가함에 따라, 사고발생 이후 준실시간 수준의 해상 유출유 현황 정보 생성이 가능해졌다. 본 연구에서는 2021년 4월 27일 중국 칭다오항 앞바다에서 발생한 심포니호 기름 유출사고를 대상으로 다종 인공위성 영상을 이용하여 기름 유출 면적을 산출하였다. 특히, 2 m 공간해상도 정보 획득이 가능한 고해상도 상용 인공위성 영상을 이용하여 기름유출 면적 산출의 정확도 향상 가능성을 평가하였다. 4월 27일부터 5월 13일까지 Sentinel-1, Sentinel-2, LANDSAT-8, GEO-KOMPSAT-2B (GOCI-II) 및 Skysat 위성영상을 수집하였으며, 기상조건을 고려하여 탐지 가능한 5장의 영상을 대상으로 유출유 탐지를 수행하였다. 유출된 기름은 사고발생 지점으로부터 남서-북동 방향으로 확산하면서, 외해에서 육지 쪽으로 이동하였다. 이러한 이동 경향은 Skysat 영상에서 확인이 가능하였으며, 사고 위치로부터 기름 입자의 이동예측을 수행한 결과와 유사하게 나타났다. 고해상도 인공위성 영상 탐지결과 및 이동예측 결과를 이용하여, 5월 1일 Sentinel-1A 영상에서 사고지점 북쪽 해역의 패치는 유사 기름으로 추정하였다. 이러한 오탐지를 제거한 결과 유출유 면적은 사고발생 후 선형적으로 증가하는 경향을 나타냈다. 본 연구 결과는 향후 고해상도 광학위성의 사용이 유출유의 분포 면적을 더욱 정확하게 산출함을 보여주었으며, 해상유출유 대응 과정에서 효율적인 방제계획 수립에 기여할 것으로 판단된다.