• 제목/요약/키워드: Resolution Limit

검색결과 284건 처리시간 0.023초

Super-resolution Microscopy with Adaptive Optics for Volumetric Imaging

  • Park, Sangjun;Min, Cheol Hong;Han, Seokyoung;Choi, Eunjin;Cho, Kyung-Ok;Jang, Hyun-Jong;Kim, Moonseok
    • Current Optics and Photonics
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    • 제6권6호
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    • pp.550-564
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    • 2022
  • Optical microscopy is a useful tool for study in the biological sciences. With an optical microscope, we can observe the micro world of life such as tissues, cells, and proteins. A fluorescent dye or a fluorescent protein provides an opportunity to mark a specific target in the crowd of biological samples, so that an image of a specific target can be observed by an optical microscope. The optical microscope, however, is constrained in resolution due to diffraction limit. Super-resolution microscopy made a breakthrough with this diffraction limit. Using a super-resolution microscope, many biomolecules are observed beyond the diffraction limit in cells. In the case of volumetric imaging, the super-resolution techniques are only applied to a limited area due to long imaging time, multiple scattering of photons, and sample-induced aberration in deep tissue. In this article, we review recent advances in super-resolution microscopy for volumetric imaging. The super-resolution techniques have been integrated with various modalities, such as a line-scan confocal microscope, a spinning disk confocal microscope, a light sheet microscope, and point spread function engineering. Super-resolution microscopy combined with adaptive optics by compensating for wave distortions is a promising method for deep tissue imaging and biomedical applications.

환경소음.진동 피해 분쟁 조정을 위한 기준설정에 관한 소고 (A brief review on the standards of regulations and compensation in the environmental noise and vibration disputes resolution)

  • 이수갑;김재환;김규태;홍지영;은희준
    • 한국소음진동공학회:학술대회논문집
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    • 한국소음진동공학회 2008년도 춘계학술대회논문집
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    • pp.876-878
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    • 2008
  • The standards of acceptable limit and compensation is one of the most important things in environmental noise and vibration disputes resolution. In this paper, review on the present acceptable limit level and compensation standard in National Environmental Dispute Resolution Commission is introduced. Discordance of standards between in the regulation law and in the dispute resolution commission and it's improvement are discussed. Abnormal reasoning for compensation standards is pointed out from a author's private view.

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전파형 역산을 이용한 시추공 영상의 분해능 (Resolution Limits of Cross-Well Seismic Imaging Using Full Waveform Inversion)

  • 조창수;이희일;서정희
    • 지구물리와물리탐사
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    • 제5권1호
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    • pp.33-45
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    • 2002
  • 주시 토모그래피의 한계인 분해능을 극복하기 위하여 새로운 영상화 기법이 요구되며 그 중의 하나가 파형 역산이다. 파형 역산은 위상뿐만 아니라 파동의 진폭을 동시에 이용하므로 지하구조를 고해상으로 영상화할 수 있는 기법이다. 그러나 파형역산은 전파와 역전파의 모형반응 계산이 요구되므로 많은 계산 시간이 요구된다. 본 연구에서는 파형역산 기법에서 효율적인 합성 파동장 계산을 위하여 속도-응력법을 이용하였다. 시추공 영상화 기법들의 분해능을 알아 보기 위하여 수치모형에 적용, 비교하여 파형역산과 주시 토모그래피의 분해능을 살펴보았다. 파형역산의 분해능 한계는 Schuster가 유도한 구조보정의 분해능 한계와 유사함을 알 수 있었다. 시추공의 기하학적인 문제로 인한 커버리지의 부족의 문제는 VSP자료를 적용함으로서 해결할 수 있어 수평적인 분해능이 향상되었다. 또한 구현된 알고리듬의 현장적용성을 평가하기 위하여 실제와 유사한 이론모형에 적용해보았으며 이 때 발생하는 비선형성을 줄이기 위해 초기치로 주시역산 토모그램을 적용하여 좋은 결과를 얻었다.

Super-Resolution Optical Fluctuation Imaging Using Speckle Illumination

  • Kim, Min-Kwan;Park, Chung-Hyun;Park, YongKeun;Cho, Yong-Hoon
    • 한국진공학회:학술대회논문집
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    • 한국진공학회 2014년도 제46회 동계 정기학술대회 초록집
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    • pp.403.1-403.1
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    • 2014
  • In conventional far-field microscopy, two objects separated closer than approximately half of an emission wavelength cannot be resolved, because of the fundamental limitation known as Abbe's diffraction limit. During the last decade, several super-resolution methods have been developed to overcome the diffraction limit in optical imaging. Among them, super-resolution optical fluctuation imaging (SOFI) developed by Dertinger et al [1], employs the statistical analysis of temporal fluorescence fluctuations induced by blinking phenomena in fluorophores. SOFI is a simple and versatile method for super-resolution imaging. However, due to the uncontrollable blinking of fluorophores, there are some limitations to using SOFI for several applications, including the limitations of available blinking fluorophores for SOFI, a requirement of using a high-speed camera, and a low signal-to-noise ratio. To solve these limitations, we present a new approach combining SOFI with speckle pattern illumination to create illumination-induced optical fluctuation instead of blinking fluctuation of fluorophore.. This technique effectively overcome the limitations of the conventional SOFI since illumination-induced optical fluctuation is possible to control unlike blinking phenomena of fluorophore. And we present the sub-diffraction resolution image using SOFI with speckle illumination.

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Real-time Speed Limit Traffic Sign Detection System for Robust Automotive Environments

  • Hoang, Anh-Tuan;Koide, Tetsushi;Yamamoto, Masaharu
    • IEIE Transactions on Smart Processing and Computing
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    • 제4권4호
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    • pp.237-250
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    • 2015
  • This paper describes a hardware-oriented algorithm and its conceptual implementation in a real-time speed limit traffic sign detection system on an automotive-oriented field-programmable gate array (FPGA). It solves the training and color dependence problems found in other research, which saw reduced recognition accuracy under unlearned conditions when color has changed. The algorithm is applicable to various platforms, such as color or grayscale cameras, high-resolution (4K) or low-resolution (VGA) cameras, and high-end or low-end FPGAs. It is also robust under various conditions, such as daytime, night time, and on rainy nights, and is adaptable to various countries' speed limit traffic sign systems. The speed limit traffic sign candidates on each grayscale video frame are detected through two simple computational stages using global luminosity and local pixel direction. Pipeline implementation using results-sharing on overlap, application of a RAM-based shift register, and optimization of scan window sizes results in a small but high-performance implementation. The proposed system matches the processing speed requirement for a 60 fps system. The speed limit traffic sign recognition system achieves better than 98% accuracy in detection and recognition, even under difficult conditions such as rainy nights, and is implementable on the low-end, low-cost Xilinx Zynq automotive Z7020 FPGA.

온라인 자동화 시료 전처리 및 HR Orbitrap LC/ESI-MS를 이용한 환경시료 중 잔류 의약물질 분석방법 확립 (Establishment of the Analytical Method for Residual Pharmaceuticals in Raw Water Using Online Sample Preparation and High Resolution Orbitrap LC/ESI-MS)

  • 황윤정;신상희;박종숙
    • 한국물환경학회지
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    • 제29권3호
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    • pp.409-419
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    • 2013
  • In this study, the analytical method for 27 residual pharmaceuticals in raw water was developed. Online sample preconcentration/extraction and analysis with high resolution Orbitrap mass spectrometry (LC-ESI/Orbitrap MS) were performed. The calibration curves showed good linearities (above $r^2$ = 0.998) in the range of 5 ~ 1,000 ng/L. The method detection limit and the limit of quantification were 1.1 ~ 10.0 ng/L and 3.4 ~ 31.7 ng/L, respectively. Recoveries of the target compounds were between 70.1% and 115.8% (except cefadroxil, cefradine, vancomycin, and iopromide (50.2 ~ 67.0%)). The optimized analytical method can be useful to determine the residual pharmaceuticals in raw water.

영상 신호에서 커브 피팅을 이용한 구조물 진동 측정 (Measuring Structural Vibration from Video Signal Using Curve Fitting)

  • 전형섭;최영철;박종원
    • 한국소음진동공학회논문집
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    • 제19권9호
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    • pp.943-949
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    • 2009
  • Many studies for measuring vibration using image signal are suggested. These methods can measure vibration of multi-points simultaneously. However, it has the disadvantage that is very sensitive to an environment. If the measured environment is not good, image signals can be measured including much background noise. So, it is difficult to obtain accurate vibration from the measured image signals. Another problem is that camera imaging has a resolution limit. Because the resolution of the camera image is relatively much lower than that of a data acquisition system, accurate measuring vibration cannot be performed. In this paper, we proposed the enhanced technique for measuring vibration using camera signal. The key word of this paper is a curve fitting. The curve fitting can exactly detect the measurement line of interested object. So, we can measure the vibration in noisy environment. Also, it can overcome the resolution limit.

Attenuated Phase Shift Mask에 광 근접 효과 보정을 적용한 고립 패턴의 해상 한계 분석 (Resolution Limit Analysis of Isolated Patterns Using Optical Proximity Correction Method with Attenuated Phase Shift Mask)

  • 김종선;오용호;임성우;고춘수;이재철
    • 한국전기전자재료학회논문지
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    • 제13권11호
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    • pp.901-907
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    • 2000
  • As the minimum feature size for making ULSI approaches the wavelength of light source in optical lithography, the aerial image is so hardly distorted because of the optical proximity effect that the accurate mask image reconstruction on wafer surface is almost impossible. We applied the Optical Proximity Correction(OPC) on isolated patterns assuming Attenuated Phase Shift Mask(APSM) as well as binary mask, to correct the widening of isolated patterns. In this study, we found that applying OPC to APSM shows much better improvement not only in enhancing the resolution and fidelity of t도 images but also in enhancing the process margin than applying OPC to the binary mask. Also, we propose the OPC method of APSM for isolated patterns, the size of which is less than the wavelength of the ArF excimer laser. Finally, we predicted the resolution limit of optical lithography through the aerial image simulation.

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조류계산을 위한 분리된 UPFC 모형에서의 제한값 해결 (Limit Resolution in the Decoupled UPFC Model for Power Flow)

  • 김태현;서장철;임정욱;문승일;박종근;한변문
    • 대한전기학회논문지:전력기술부문A
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    • 제48권7호
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    • pp.824-831
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    • 1999
  • This paper presents new methods to resolve the important limits in the decoupled UPFC model for power flow, by which conventional power flow program can be performed with addition of two buses per one UPFC. In order to operate UPFC to the desired value, the series voltage and shunt current of UPFC should be computed. So a method of calculating these by simple equations after power flow is derived. However, the calculated magnitude of series voltage and/or shunt current of UPFC may not be allowed because of the UPFC limit \ulcorner to the ratings of inverters. In this case, the active power and the reactive power (or the voltage magnitude) of UPFC buses should be revised to resolve the limit. This paper proposes the Newton Raphson method to resolve these limits. Particularly, when resolving the series voltage magnitude, three strategies are proposed according to the priority of the active power and the reactive power (or the voltage magnitude).

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Evaluation of Resolution Improvement Ability of a DSP Technique for Filter-Array-Based Spectrometers

  • Oliver, J.;Lee, Woong-Bi;Park, Sang-Jun;Lee, Heung-No
    • 한국통신학회논문지
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    • 제38C권6호
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    • pp.497-502
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    • 2013
  • In this paper, we aim to evaluate the performance of the digital signal processing (DSP) algorithm used in [8] in order to improve the resolution of spectrometers with fixed number of low-cost, non-ideal filters. In such spectrometers, the resolution is limited by the number of filters. We aim to demonstrate via new experiments that the resolution improvement by six times over the conventional limit is possible by using the DSP algorithm as claimed by [8].