• The Plant Pathology Journal
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• v.37 no.6
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• pp.555-565
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• 2021

Bacteriophages infecting Acidovorax citrulli, the causal agent of bacterial fruit blotch, have been proven to be effective for the prevention and control of this disease. However, the occurrence of bacteriophage-resistant bacteria is one of hurdles in phage biocontrol and the understanding of phage resistance in this bacterium is an essential step. In this study, we aim to investigate possible phage resistance of A. citrulli and relationship between phage resistance and pathogenicity, and to isolate and characterize the genes involved in these phenomena. A phage-resistant and less-virulent mutant named as AC-17-G1 was isolated among 3,264 A. citrulli Tn5 mutants through serial spot assays and plaque assays followed by pathogenicity test using seed coating method. The mutant has the integrated Tn5 in the middle of a cupin protein gene. This mutant recovered its pathogenicity and phage sensitivity by complementation with corresponding wild-type gene. Site-directed mutation of this gene from wild-type by CRISPR/Cas9 system resulted in the loss of pathogenicity and acquisition of phage resistance. The growth of AC-17-G1 in King's B medium was much less than the wild-type, but the growth turned into normal in the medium supplemented with D-mannose 6-phosphate or D-fructose 6-phosphate indicating the cupin protein functions as a phosphomannos isomerase. Sodium dodecyl sulfa analysis of lipopolysaccharide (LPS) extracted from the mutant was smaller than that from wild-type. All these data suggest that the cupin protein is a phosphomannos isomerase involved in LPS synthesis, and LPS is an important determinant of pathogenicity and phage susceptibility of A. citrulli.

• Journal of Ocean Engineering and Technology
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• v.30 no.2
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• pp.69-74
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• 2016

A new wave-piercing-type high-speed planing boat without a chine was developed, and its basic performance was investigated in a model test, including the resistance, trim, and sinkage. The maximum speed of the developed ship was 35 knots. The hull form was developed by combining a VSV (very slender vessel) and TH (transonic hull), which have large deadrise angles at the bow. The main dimensions were estimated by a statistical approach using actual ship data. The effect of a side fin attached at the stern near the water line was investigated from a resistance point of view. It was found that the developed hull form showed the possibility of a new concept for a high-speed planing hull without a chine, and the side fin played an important role in increasing the resistance performance by controlling the trim and sinkage in the high-speed range.

• Journal of Plant Biotechnology
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• v.40 no.1
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• pp.49-54
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• 2013

C3HC4-type RING zinc finger proteins essential in the regulation of plant processes, including responses to abiotic stresses. We previously isolated and examined the C3HC4-type RING zinc finger protein (BrRZFP1) from Brassica rapa under abiotic stresses. To elucidate the role of the BrRZFP1 transcription factor in gene regulation, we transformed tobacco plants with the BrRZFP1 gene. Plants were regenerated from 82 independently transformed callus lines of tobacco and analysed for transgene expression. Transgene integration and expression was confirmed by Southern and RT-PCR analyses, respectively. T2 plants displayed more tolerance to the bacterial pathogens Pectobacterium carotovorum and Ralstonia solanacearum, and the tolerance levels were correlated with BrRZFP1 expression levels. These results suggest that the transcription factor BrRZFP1 is an important determinant of stress response in plants and its overexpression in plants could increase biotic stress resistance.

• Journal of the Society of Disaster Information
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• v.10 no.1
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• pp.61-70
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• 2014

Synthetic fiber reinforced concrete is applicable to many applications for construction material. In general, synthetic fibers have low tensile strength and elastic modulus, but they have many advantages such as high crack resistance, impact resistance, chemical resistance, flexural behavior and corrosion free in fiber reinforced concrete. Recently, fiber reinforced concrete with macro synthetic fibers has been used to improve performance of structures in tunnel shotcrete, precast segmental lining and bridge slab and precast concrete structures. This study investigated the influence of bundled type polyamide fiber reinforced concrete on the flexural behavior in accordance with ASTM C 1609 and KS F 2566 standards.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2008.06a
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• pp.208-209
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• 2008

Ni/Ti/Al multilayer system was tested for low-resistance ohmic contact formation to Al-implanted p-type 4H-SiC. Compared with conventional process using Ni, Ni/Ti/Al contact shows perfect ohmic behavior, and possesses much lower contact resistance of about $2.5\times10^{-4}\Omega{\cdot}cm^2$ after $930^{\circ}C$ RTA, which is about 2 orders of magnitude smaller than that of Ni contact. Contact resistance gradually increased as the RTA temperature was lowered in the range of 840 ~ $930^{\circ}C$, and about $3.4\times10^{-4}\Omega{\cdot}cm^2$ was obtained at the lowest RTA temperature of $840^{\circ}C$. Therefore, it was shown that RTA temperature for ohmic contact formation can be lowered to at least $840^{\circ}C$ without significant compromise of contact resistance.

• The Transactions of the Korean Institute of Electrical Engineers
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• v.37 no.10
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• pp.694-701
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• 1988

Distributed-type railguns are designed to maintain the armature current and the length between the armature and the current-feed region nearly constant with time. This paper deals with factors affecting current distribution, effective resistance and effective skin depth in the rail of a distributed-type railgun. Analytical solutions for the current distributions and resistance in the rail are presented for a simple two-dimensional model under steady-state contions. For diffusion limited current, it is found that effective rail resistance is proportional to the square root of the relative velocity, the permeability of the rail and the length between the armature and that effective skin depth of the rail is proportional to the square root of the length and inversely proportional to the square root of the permeability, the conductivity and the velocity.

• Journal of the Korean Institute of Electrical and Electronic Material Engineers
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• v.17 no.2
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• pp.171-177
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• 2004

Voltage step-down characteristics in Ring/Dot type piezoelectric transformer were examined as a function of the area of input electrode when the area of output electrode is fixed. The effects of driving frequency and load resistance on the voltage step-down characteristics were also examined. Voltage gain was greatly dependent on the driving frequency and load resistance, and showed a maximum gain at resonance frequency of the step-down transformer. The frequency where the maximum cutout voltage appears increased about 0.2％ as the load resistance increased from 10 to 150 Ω. As the area of input electrode increased, the voltage gain and the efficiency of the transformer increased. Frequency dependence of efficiency of the step-down transformer revealed a similar tendency with the voltage gain curves. The maximum efficiency remarked 94% when the input voltage and the load resistance were 20 Vpp and 120 Ω, respectively.

• Proceedings of the Korea Concrete Institute Conference
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• 2004.11a
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• pp.553-556
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• 2004

This paper is to investigate the spalling and fire endurance of high performance RC column member with PP fiber and lateral confinement of metal lath and non fire resistance removal type form. According to test results, combination of PP fiber and metal lath as well as use fire resistance non removal type form had favorable fire resistance by discharging internal vapour pressure and lateral confining. After fire endurance test, compressive strength decreased markedly caused by internal expansion pressure and crack. Residual strength of plain concrete was decreased to $22\%$. The use of PP fiber and lateral confinement of metal lath and non removal type form enhanced the residual strength above $40\%$. Especially, the combination of $0.1\%$ of PP fiber and lateral confinement with the level of 2.3T exhibited more than $51\%$ of residual strength. Therefore, to improve fire endurance and spalling resistance, the combination of $0.1\%$ of PP fiber and metal lath with 2.3T can be the proper measure.

• Proceedings of the Korean Institute of Electrical and Electronic Material Engineers Conference
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• 2004.07b
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• pp.722-725
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• 2004

Voltage step-down characteristics in Ring/Dot type piezoelectric transformer were examined as a function of the area of input electrode when the area of output electrode is fixed. The effects of driving frequency and load resistance on the voltage step-down characteristics were also examined. Voltage gain was greatly dependent on the driving frequency and load resistance, and showed a maximum gain at resonance frequency of the step-down transformer. The frequency where the maximum output voltage appears increased about 0.2% as the load resistance increased from 10 to $150\Omega$. As the area of input electrode increased, the voltage gain and the efficiency of the transformer increased. Frequency dependence of efficiency of the step-down transformer revealed a similar tendency with the voltage gain curves. The maximum efficiency remarked 94% when the input voltage and the load resistance were 20 $V_{PP}$ and $120\Omega$, respectively.

• Journal of Microbiology and Biotechnology
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• v.20 no.3
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• pp.637-642
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• 2010

We investigated the prevalence and the molecular characteristics of vancomycin-intermediate Staphylococcus aureus (VISA) among methicillin-resistant Staphylococcus aureus (MRSA) strains isolated from clinical samples at tertiary or general hospitals participating in a nationwide surveillance program for VISA and vancomycin-resistant Staphylococcus aureus (VRSA) in Korea during an 8-week period in each year from 2001 to 2006. Of 41,639 MRSAs isolated, 37,856 were screened and 169 grew on brain heart infusion agar supplemented with 4 ${\mu}g/ml$ vancomycin. A vancomycin MIC of 4 ${\mu}g/ml$ was confirmed for 33 VISA isolates of the 169 isolates. Eighteen of the 33 isolates were classified as hetero-VISA (hVISA) by the population analysis profile (PAP) method. All VISA isolates were susceptible to linezolid, tigecycline, and quinupristin-dalfopristin. Most VISA isolates (MIC 4 ${\mu}g/ml$) showed a PFGE C pattern with sec, seg, and sei enterotoxin genes, including ST5-SCCmec type II, or a PFGE A pattern with sea, including ST239-SCCmec type III.