• Asian Pacific Journal of Cancer Prevention
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• 제14권9호
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• pp.4983-4988
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• 2013

Objectives: To establish a taxol-resistant cell line of human ovarian carcinoma (A2780/Taxol) and investigate its biological features. Methods: The drug-resistant cell line (A2780/Taxol) was established by continuous stepwise selection with increasing concentrations of Taxol. Cell morphology was assessed by microscopy and growth curves were generated with in vitro and in vivo tumor xenograft models. With rhodamine123 (Rh123) assays, cell cycle distribution and the apoptotic rate were analyzed by flow cytometry (FCM). Drug resistance-related and signal associated proteins, including P-gp, MRPs, caveolin-1, PKC-${\alpha}$, Akt, ERK1/2, were detected by Western blotting. Results: A2780/Taxol cells were established with stable resistance to taxol. The drug resistance index (RI) was 430.7. Cross-resistance to other drugs was also shown, but there was no significant change to radioresistance. Compared with parental cells, A2780/Taxol cells were significantly heteromorphous, with a significant delay in population doubling time and reduced uptake of Rh123 (p<0.01). In vivo, tumor take by A2780 cells was 80%, and tumor volume increased gradually. In contrast, with A2780/Taxol cells in xenograft models there was no tumor development. FCM analysis revealed that A2780/Taxol cells had a higher percentage of G0/G1 and lower S phase, but no changes of G2 phase and the apoptosis rate. Expression of P-gp, MRP1, MRP2, BCRP, LRP, caveolin-1, PKC-${\alpha}$, Phospho-ERK1/2 and Phospho-JNK protein was significantly up-regulated, while Akt and p38 MARK protein expression was not changed in A2780/Taxol cells. Conclusion: The A2780/Taxol cell line is an ideal model to investigate the mechanism of muti-drug resistance related to overexpression of drug-resistance associated proteins and activation of the PKC-${\alpha}/ERK$ (JNK) signaling pathway.

• 한국수산과학회지
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• 제40권2호
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• pp.73-78
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• 2007

The suitability of a flexible fiber filter for removing suspended solid (SS) in a recirculating aquaculture system was evaluated. This study focused on variation in the performance with a change in filtering time, influent water quality, and filtering mode duration. The particle distribution diagram of the filter effluent showed that the number of particles bigger than $5-8{\mu}m$ decreased dramatically, and the removal efficiency exceeded 80%. Although the removal efficiencies of SS and chemical oxygen demand (COD) were dependent on the quality of the influent, the SS and COD concentrations of the effluent were not affected by the influent concentrations. This was despite the deterioration if water quality after feeding in the rearing tank. The performance of the filter was not affected by the filtering mode duration, feeding conditions, or filtering time. The SS concentration and turbidity of the recirculating-type rearing tank were 30% and 50% lower, respectively, than of the a non-recirculating-type rearing tank under the same operating conditions. The flexible fiber filter was applicable to a recirculating aquaculture system that uses plenty of seawater, based on its low filtering resistance $(2kg_f/cm^2)$, high flux $(330m^3/m^2/hr)$, and high fine particle removal efficiency (80%, $5-8{\mu}m$).

• 대한의용생체공학회:학술대회논문집
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• 대한의용생체공학회 1993년도 추계학술대회
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• pp.51-54
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• 1993

The purpose of this research is to investigate the relationship between the blood flow and autonomic nervous system which regulates the circulation system. After the oral glucose tolerance test(GTT) the velocity changes of the blood flow were measured from the normal, hyperglycemia and diabetic neuropathy using the laser doppler. The FFT analyses showed that the spectrums were concentrated around the particular bands(0.02-0.166Hz, 0.8-1.1Hz) for the normal, while almost uniform distribution of the spectrums was observed for the abnormal. The blood glucose affects the compliance of the blood vessels and viscosity and finally causes the resistance problem in the blood vessels. An index PG was introduced, which was determined by the ratio of the change or the integral power spectrum in the range between 0.02 and 0.166Hz to change of the blood glucose before and after GTT. The index shows 1.0. 0.3 and 0.1 for the normal, hyperglycemia and diabetic neuropathy, respectively. As a conclusion, this spectrum analysis shows the potential of checking the progress or the diabetic, also car be used to evaluate the vasomotion which are regulated by the autonomic nervous system.

• 한국정밀공학회지
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• 제15권1호
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• pp.43-50
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• 1998

The purpose of the paper is to protect the damage of plastic IC package with searching the cause of the fracture due to the delamination and crack when the encapsulant of plastic IC package is on viscoelastic behavior with the effect of creep on high temperature, The model for analysis is the plastic SOJ package with dimpled diepad in the IR soldering process of surface mounting technology. The risk of delamination with calculating the distribution of viscoelastic thermal stress in the package without the crack in the surface mounting process is checked. The package model with the perfect delamination between chip and diepad is chosen to estimate the resistance against fracture in thermal loading with calculating C (t)-integrals according to the change of the design. The optimum design to depress the delamination and crack is presented.

• 펄프종이기술
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• 제39권5호
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• pp.32-38
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• 2007

The purpose of this study is to investigate the cause of fine wrinkles occurred in silky wallpaper and its solution. The characteristics of base papers, wallpapers and adhesives (conventional and modified) were evaluated to find the possibility for the occurrence of fine wrinkles. It was found that the base papers and wallpapers did not cause the occurrence of silky wallpaper. It was concluded that the fine wrinkles in silky wallpaper was caused by the nonuniform distribution of adhesive, the localized shrinkage of adhesive and the breaking the balance between the resistance of wallpaper and shrinkage of adhesive. Three possible solutions for the prevention of the occurrence of fine wrinkles in silky paper were recommended.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2005년도 International Meeting on Information Displayvol.I
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• pp.714-716
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• 2005

We studied the dependence of the electrical characteristics of MgO protecting layer on temperature in an AC PDP cell. Careful measurements of the surface resistance of MgO were performed for the temperature range from room temperature to $100^{\circ}C$ with and without the VUV illumination. Experimental results show that the resistivity is affected by not only the temperature but also VUV irradiation. The measurement of wall charge distribution and the address discharge delay time as to the temperature show that the resistivity change of MgO may affect the wall voltage and consequently the discharge delay time.

• 설비공학논문집
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• 제6권2호
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• pp.67-77
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• 1994

In this study, one-dimensional Stefan problem with air-gap resistance in the rectangular mold is considered and the thermal characteristics are examined by using the enthalpy-based simple implicit finite-difference scheme. The enthalpy and temperature are nondimensionalized to obtain general solutions. The temperature distribution and the locations of solidus and liquidus line are obtained and the effects of major parameters on the thermal characteristics are investigated.

• Structural Engineering and Mechanics
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• 제86권3호
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• pp.325-336
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• 2023

A significant component of many civil constructions such as buildings, reservoirs, bridges, and sports halls, concrete has become increasingly popular due to its versatile properties. Concrete's internal characteristics change due to the use of different types of fibers, including changes in its microstructure, volume, and hole dimensions. Additionally, the type, dimensions, and distribution of fibers in concrete can affect the results of flexural strength tests by affecting its compressive and tensile strength. Due to a lack of information, fiber concrete is a new composite material in the production industry that requires laboratory studies to determine its behavior. This study investigated the bending behavior of multilayer slabs made of concrete reinforced by polyamide-propylene fibers against impact in weight lifting exercises. Results showed that adding fibers to concrete slab samples improved the mechanical properties while replacing them hurt the mechanical properties and failure of polymer fiber-reinforced concrete. On the other hand, adding and replacing fibers increases durability and has a positive effect.

• Elastomers and Composites
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• 제57권4호
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• pp.165-174
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• 2022

The effect of fumed silica loading on the thermal stability and mechanical properties of fluorosilicone (FVMQ) rubber was investigated. The distribution of fumed silica inside FVMQ was characterized using scanning electron microscopy, and the thermal stability of composites was evaluated using thermogravimetric analysis and by the changes in mechanical performance during thermo-oxidative aging. The function mechanism of fumed silica was studied by Fourier transform infrared spectroscopy. The results show that with increasing silica content, the crosslink density of composites, the modulus at 100%, and tensile strength also increased, whereas the elongation at break decreased. Furthermore, increasing the silica content of composites increased the initial decomposition temperature (T_d) and residual weight of the composite after exposure to nitrogen. In addition, the thermal oxidative aging experiment demonstrated improved aging resistance of the FVMQ composites, including lower change in tensile strength, elongation at break, and modulus at 100%.

• 대한조선학회논문집
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• 제60권1호
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• pp.1-9
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• 2023

In order to improve the propeller cavitation performance composed of Cavitation Inception Speed (CIS), cavitation extent and pressure fluctuation, it needs to improve the wake distribution that flows into the propeller. The warship propeller cavitation is strongly influenced by the wake created at the V-strut of various appendages. The inflow characteristics of the V-strut were investigated using Computational Fluid Dynamics (CFD) and the twisted angles of the V-strut were aligned with upstream flow. The resistance and self-propulsion tests for the model ship with the existing and modified V-struts were conducted in Towing Tank (TT), and wake distribution, CIS, cavitation observation and pressure fluctuation tests were conducted in Large Cavitation Tunnel (LCT). The propeller behind the modified V-strut showed better cavitation characteristics than that behind the existing V-strut. Another model test was conducted to investigate rudder cavitation performance by the change of the V-strut. The rudder cavitation characteristics were not improved by the change of the operating conditions. On the basis of the present study, it is thought that the stern appendages for better propeller cavitation performance would be developed.