• Restorative Dentistry and Endodontics
• /
• v.42 no.1
• /
• pp.39-47
• /
• 2017

Objectives: Although the coating of surface sealants to dental composite resin may potentially reduce bacterial adhesion, there seems to be little information regarding this issue. This preliminary in vitro study investigated the adhesion of Streptococcus mutans (S. mutans) on the dental composite resins coated with three commercial surface sealants. Materials and Methods: Composite resin (Filtek Z250) discs (8 mm in diameter, 1 mm in thickness) were fabricated in a mold covered with a Mylar strip (control). In group PoGo, the surfaces were polished with PoGo. In groups PS, OG, and FP, the surfaces polished with PoGo were coated with the corresponding surface sealants (PermaSeal, PS; OptiGuard, OG; Fortify Plus, FP). The surfaces of the materials and S. mutans cells were characterized by various methods. S. mutans adhesion to the surfaces was quantitatively evaluated using flow cytometry (n = 9). Results: Group OG achieved the lowest water contact angle among all groups tested (p < 0.001). The cell surface of S. mutans tested showed hydrophobic characteristics. Group PoGo exhibited the greatest bacterial adhesion among all groups tested (p < 0.001). The sealant-coated groups showed statistically similar (groups PS and FP, p > 0.05) or significantly lower (group OG, p < 0.001) bacterial adhesion when compared with the control group. Conclusions: The application of the surface sealants significantly reduced S. mutans adhesion to the composite resin polished with the PoGo.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.25 no.2
• /
• pp.277-284
• /
• 1998

For the purpose of comparing the shear bond strengths of pit and fissure sealants, and finding out the more efficient method of tooth surface treatment when the etched surface is contaminaed by saliva or moisture, an experiment was performed on 3 types of pit and fissure sealants. 120 extracted human molars were divided into 3 groups, each of which was composed of 40 specimens sealed with Helioseal, Teethmate-F and Fuji III respectively. And each groups was again divided into 4 subgroups according to tooth surface treatment. The shear bond strengths of each groups and subgroup was measured and statistically analyzed. The results obtained were as follows : 1. Shear bond strengths of nonfluoridated resin sealant, Helioseal were shown to be higher than those of fluoridated resin sealant, Teethmate-F, but, not significantly different. 2. Shear bond strengths of GI sealant, Fuji III were to be markedly lower than those of two resin sealants. 3. When there is moisture contamination, applying primer under sealant(Group IV) results in a significantly stronger bond strength of sealant to enamel than when using sealant alone(Group II) in case of all sealants. 4. When there is no moisture contamination, using primer under sealant (Group III) results in bond strength equivalent to bond strength on using sealant alone (Group I). 5. Based on the results above, it was demonstrated that the bond of sealant to tooth surface is greatly affected by saliva contamination and that the complete tooth isolation method should be fully emphasized. The application of primer is recommended when performing sealant under the environment very susceptible to saliva contamination.

• Restorative Dentistry and Endodontics
• /
• v.31 no.4
• /
• pp.282-289
• /
• 2006

The purpose of this study was to compare the ability of three resin surface sealants to prevent microleakage in Class V composite resin restorations. Forty Class V cavities with the occlusal margin in enamel and gingival margin in dentin were prepared on the buccal surfaces of sound extracted molars, and restored with composite resin. Restorations were randomly assigned into one of four equal groups (n = 10): a control group, without resin sealing, and three experimental groups in which margins were sealed with Fortify Plus, Biscover and Permaseal, respectively. Specimens were thermocycled, immersed in a 2% methylene blue solution for 4 hours, sectioned longitudinally, and observed the leakage at the occlusal and gingival margins. The result was analyzed using Kruskal-Wallis test, Mann-Whitney test and Wilcoxon signed rank test. In conclusion, the ability to reduce microleakage at occlusal margins was similar in all of three sealants. However at gingival margin, it depended on the type of used resin surface sealant. At gingival margin. control and Fortify Plus group showed statistically higher microleakage than PermaSeal group. and Fortify Plus group also showed higher microleakage than BisCover group (p < 0.05).

• The Journal of Korean Academy of Prosthodontics
• /
• v.47 no.2
• /
• pp.182-190
• /
• 2009

Statement of problem: Microleakage at the occlusal and gingival margin of Class V cavities restored with composite resin has traditionally been considered an obstacle to successful restoration. Purpose: The aim of this study was to assess the effectiveness of three different surface sealants(Fortify, Permaseal and Biscover LV) on the marginal sealing of Class V light-activated composite resin restorations(Z250). Material and methods: Forty noncarious human premolars and molars extracted within a three-month period were selected. Class V cavities with the occlusal margin in enamel and gingival margin in cementum were prepared in both buccal and lingual surfaces. The teeth, randomly assigned in four groups with twenty cavities in each group, were restored with composite resin after applying an adhesive system(Clearfil SE bond). After the finishing and polishing procedures, the restorations were covered with a specific surface sealants, except for the control samples, which were not sealed. After placing restorations, the specimens were thermocycled, and immersed in a 2% methylene blue solution for twenty four hours and sectioned longitudinally. The marginal microleakage was evaluated at the occlusal and gingival interfaces using a microscope and compared among the four groups using ANOVA test and Wilcoxon Rank-Sum test($\alpha$=0.05). Results: Statistical analysis showed that there was significantly less leakage when the surface sealants were used than there was in control group(P<.05). There were no significant differences of microleakage at occlusal and gingival margins among groups. There were no significant differences between microleakage of occlusal and gingival margins in each group. Fortify was not statistically different from control group at the gingival margin(P>.05). Conclusion: Application of surface sealants was an effective method of surface coating in reducing microleakage at occlusal and gingival margins of Class V composite resin restorations. However, it is certain that some microleakage still occurred despite the application of surface sealants, especially gingival margins.

• Journal of Dental Rehabilitation and Applied Science
• /
• v.39 no.4
• /
• pp.195-203
• /
• 2023

Purpose: This study aimed to evaluate the influence of surface sealants on the surface roughness of composite resins. Materials and Methods: The study used microfilled composite resin (Metafil CX, Sun Medical Co.) and hybrid composite resin (Aelite^TM LS posterior, Bisco). Sixty specimens (8 mm in diameter and 4 mm in height) of each composite resin type were prepared and divided into 3 groups. Each specimen was ground with 600, 1000, and 2000-grit sandpaper. The Surface roughness (Ra) values were measured using a surface roughness tester (SJ-301, Mytutoyo) before and after surface sealant application. Surface sealants, BisCover^TM LV (Bisco), Optiguard^® (Kerr), and Seal-n-Shine^TM (Pulpdent), were applied to the specimens, as instructed and observed by scanning electron microscope (JSM-7500, JEOL) and atomic force microscope (MultiMode IV, Veeco Instruments). Results: Specimens ground with 600-grit sandpaper coated with surface sealants exhibited significantly lower Ra values than the untreated group (P < 0.05). Specimens ground with 1000 and 2000-grit sandpaper showed statistically no difference. There was no significant difference in surface roughness among BisCover^TM LV, Optiguard^®, and Seal-n-Shine^TM. SEM and AFM revealed remarkably decreased microdefects on the surfaces of composite resins after surface sealant application. Conclusion: Surface sealants can influence surface roughness when applied on the rough surface of composite resins but not on highly polished composite resins.

• Journal of dental hygiene science
• /
• v.18 no.6
• /
• pp.367-373
• /
• 2018

The consumption of beverages among children is rising. The purpose of this study was to examine the effect of kid's drink on dental resin-based pit and fissure sealant. Pororo, I-kicker, Sunkist kids were included in the experimental groups, and Samdasu was included in the control group. A conventional dental sealant material ($Clinpro^{TM}Sealant^{(R)}$) was selected for this study. Resin specimens (8 mm in diameter and 1 mm in thickness) were prepared according to manufacturers' instructions and the initial roughness (Ra) was then measured. The pH of all the four groups was measured using a pH meter. The specimens were individually immersed in 5 ml of the experimental solutions and stored at $37^{\circ}C$ for 72 hours. Following this, the surface roughness of the resin specimens was measured by Surftest. The concentration of residual monomer released was determined by high performance liquid chromatography (HPLC). The surface morphology of the resin specimen was evaluated before and after storage by scanning electron microscopy (SEM). Data were statistically analyzed using Kruskal-Wallis and Duncan's test. The results showed that all the children's beverages examined in this study contained citric acid. The pH of I-kicker was the lowest ($3.03{\pm}0.01$), followed by that of Sunkist kids ($3.26{\pm}0.02$) and Pororo ($3.47{\pm}0.02$). We observed an increase in the surface roughness of resin specimens after 72 h of immersion in all the beverages tested (p<0.05). There was matrix degradation after immersion, visualized on SEM image, in all the beverage groups. Bisphenol-A-glycidyl methacrylate was not detected after 72 hours, but triethylene glycol dimethacrylate levels were increased in all the beverages tested during the 72 hours by HPLC. These results suggest that intake of beverages containing acid can cause degradation of the resin-based pit and fissure sealants in children.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.24 no.2
• /
• pp.460-474
• /
• 1997

The purpose of this study was to evaluate and compare the effectiveness of various low-viscosity resin systems used as rebonding agents to prevent microleakage at the margins of class I composite resin restorations. Seventy sound human premolars were selected for experiment. Class I cavities were prepared and each cavity was conditioned with a 37% phosphoric acid for 15 sec, rinsed with water for 15 sec, and dried with compressed air. Bonding agent(Scotchbond Multipurpose, 3M Co.) was applied and a hybrid composite resin (Z-100, 3M Co.) was placed using an incremental technic. The excess cured composite resin was carefully removed with Sof-Lex discs(3M Co.) to expose the original margins of the cavity. The following seven groups were established : group 1 was not rebonded and used as control group ; group 2 was rebonded with a Scotchbond Multipurpose(3M Co.) and finished ; group 3 was rebonded with a Fortify(BISCO) and finished ; group 4 was rebonded with a Concise white sealant(3M Co.) and finished ; group 5 was rebonded with a Concise white sealant(3M Co.) and not finished ; group 6 was rebonded with a P&F sealant(BISCO) and finished; group 7 was rebonded with a P&F sealant(BISCO) and not finished. The specimens were then subjected to 500 thermocycles between 5 & 65 with a 10 see dwell time and immersed in 2% methylene blue dye solution for 24 hours and sectioned with low-speed diamond cutter into two part under water condition. The extent of microleakage at rebonded margins was evaluated microscopically and scored for dye penetration according to the following scale : 0=no dye penetration ; 1=dye penetration to half-way along axial wall between enamel surface and DEJ ; 2=dye penetration beyond halfway along axial wall between enamel surface and DEJ ; 3=dye penetration to the full depth of DEJ or beyond DEJ. Selected samples were prepared for SEM observation to determine the depth of penetration of the rebonding agent into the marginal interface. The obtained results were as follows: 1. In the group 2 and 3, which is rebonded with a Scotchbond Multipupose and Fortify, dye penetration score were decreased significantly than that of group 1 (P<0.05), but group 4 and 6 were not statistically different from group 1(P>0.05). 2. There were significant differences between group 4, 6 and group 5, 7 when compared by dye penetration score (P<0.05). 3. In the SEM observation, Scotchbond Multipurpose and Fortify were penetrated within $30-40{\mu}m$ depth of the outermost surface. However, both sealants were failed to penetrate into the debonded interface.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.28 no.3
• /
• pp.512-521
• /
• 2001

Recently. the reintroduction of air-abrasion technology in dentistry has added a new potential method of pretreating teeth prior to placing sealants. The purpose of this in vitro study was to investigate microleakages of several pit and fissure sealants following various surface pre-treatment methods to the enamel, because there was a question concerning the validity of claim that this method was better than the conventional acid etching. Permanent molars were divided randomly into nine groups and treated accordingly: acid etching(group 1, 4, 7), air-abrasion(group 2, 5, 8), acid etching after air-abrasion(group 3, 6, 9). Then the authors placed and cured the three kinds of sealants(group $1\sim3$: Teethmate-F, group $4\sim6$: Ultraseal XT plus, group $7\sim9$: Denseal) according to the manufacturer's order. They were observed to determine the degree of microleakage. And these specimens were separated from the corresponding enamels and examined by Scanning Electron Microscope. The following results were obtained: 1. In comparing microleakage among tooth surface treatment methods, air-abrasion group(group 2, 5, 8) showed the greatest microleakage, while combination(air-abrasion + acid etching) group showed the least (p<0.05). However, no significant differences were found between group 7 and 9. 2. The mean microleakages were ranked as follows(p<0.05): In acid etching groups, group 7> group 4> group 1. In air-abrasive groups, group 8>groups 5> group 2. In combination groups, group 9>group 3> group 6. However, no significant differences were found between group 4 and 7 between group 2 and 5 and between group 3 and 6. 3. SEM showed that comparably longer resin tags were distributed regularly in acid etching groups and that shorter ones irregularly in air-abrasion groups. It also showed that these two kinds of tags were distributed simultaneously in combination groups.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.43 no.4
• /
• pp.347-353
• /
• 2016

The purpose of this study was to evaluate shear bond strength and microleakage of a new self-etch fissure sealant containing S-PRG filler (BeautiSealant). Fourty-five premolars were randomly divided into three groups: SEPBS group (Self-etching primer + BeautiSealant), AEBS group (Phosphoric acid etching + BeautiSealant), AECP group (Phosphoric acid etching + $Clinpro^{TM}$ sealant). For the shear bond strength test, microcylinders of fissure sealant were bonded to prepared buccal surfaces of teeth and shear bond strengths were measured by a universal testing machine. For the microleakage test, sealants were applicated on each occlusal fissure of teeth. All teeth surface were coated with finger nail varnish, with the exception of a 1.0 mm window around the sealant margins. The teeth were immersed in 2% methylene blue solution for 24 hours and then rinsed in water. The teeth were then embedded in resin and cut buccolingually along the tooth axis and observed with a stereomicroscope to determine the degree of microleakage. SEPBS group demonstrated lower bond strength in comparison with that of AEBS and AECP groups (p < 0.05). However, no significant differences were observed between AEBS and AECP groups. There was no statistically significant difference in degree of microleakage among three groups.

• Journal of the korean academy of Pediatric Dentistry
• /
• v.33 no.4
• /
• pp.633-642
• /
• 2006

This study evaluated the surface changes such as surface average roughness(RA), surface free energy(SFE) and wear depth among groups treated with surface penetrating sealant(Fortify, Biscover), non treated polishing group before and after tooth-brushing, analyzing the influence of the sealants in filling of surface microdefects formed during the finishing and polishing procedures of composite resin. Results were as follow(p= 0.05) : 1. Ra was increased Biscover. Fortify and Polishing group. Before abrasion and in 6 months, all groups were statistically significant. In 1 month, 2 months and 3 months no statistical difference was noticed between Fortify and Biscover 2. SFE of Polishig and Biscover group. Fortify and Biscover group were statistically significant before abrasion. SFE of Polishing and Fortify group was statistically significant in 1 month. No statistical difference was noticed among groups in 2 months. SFE of Fortify and Biscover group was statistically significant in 3 months. Polishing and Fortify group, Fortify and Biscover group were statistically significant in 6 months. 3, Wear rate between Polishing and Fortify group was statistically significant in only 1 month. Wear rate between Polishing and Biscover group was statistically different in each month except for 2 month and so it was between Fortify and Biscover group. Considering film thickness of Fortify and Biscover, Fortify almost discappeared after 2 months and Biscover did after 6 months.