• Archives of Pharmacal Research
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• 제26권10호
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• pp.846-854
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• 2003

The oligomerization of G-proteincoupled receptors (GPCRs) has been shown to occur by various mechanisms, such as via disulfide covalent linkages, non covalent (ionic, hydrophobic) interactions of the N-terminal, and/or transmembrane and/or intracellular domains. Interactions between GPCRs could involve an association between identical proteins (homomers) or non-identical proteins (heteromers), or between two monomers (to form dimers) or multiple monomers (to form oligomers). It is believed that muscarinic receptors may also be arranged into dimeric or oigomeric complexes, but no systematic experimental evidence exists concerning the direct physical interaction between receptor proteins as its mechanism. We undertook this study to determine whether muscarinic receptors form homomers or a heteromers by direct protein-protein interaction within the same or within different subtypes using a yeast two-hybrid system. Intracellular loops (i1, i2 and i3) and the C-terminal cytoplasmic tails (C) of human muscarinic (Hm) receptor subtypes, Hm1, Hm2 and Hm3, were cloned into the vectors (pB42AD and pLexA) of a two-hybrid system and examined for heteromeric or homodimeric interactions between the cytoplasmic domains. No physical interaction was observed between the intracellular domains of any of the Hm/Hm receptor sets tested. The results of our study suggest that the Hm1, Hm2 and Hm3 receptors do not form dimers or oligomers by interacting directly through either the hydrophilic intracellular domains or the C-terminal tail domains. To further investigate extracellular domain interactions, the N-terminus (N) and extracellular loops (o1 and o2) were also cloned into the two-hybrid vectors. Interactions of Hm2N with Hm2N, Hm2o1, Hm2o2, Hm3N, Hm3o1 or Hm3o2 were examined. The N-terminal domain of Hm2 was found to have no direct interaction with any extracellular domain. From our results, we excluded the possibility of a direct interaction between the muscarinic receptor subtypes (Hm1, Hm2 and Hm3) as a mechanism for homo- or hetero-meric dimerization/oligomerization. On the other hand, it remains a possibility that interaction may occur indirectly or require proper conformation or subunit formation or hydrophobic region involvement.

• 가정과삶의질연구
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• 제32권5호
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• pp.145-161
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• 2014

The purpose of this study examined the differences in work-family spillover process and domains in dual-earner couples with children, using a multidimensional measure of work-family spillover. The subjects of this study were 285 working couples with children. The research tool was questionnaires which consisted of general characteristics of the subjects and multidimensional measure of work-family spillover scale. For data analysis, factor analysis, Cronbach ${\alpha}$, t-test, and paired t-test were performed. Based on analysis of data using factor analysis, three-distinct work-family spillover process can be measured for both wife and husband, time interference, psychological distraction, and energy depletion and the multidimensional measure of work-family spillover is able to validly assess different domains of family life into which spillover occurs. The main results of this study were as following: First, there were significant differences in work-family spillover process according to sex. In work${\rightarrow}$family spillover, male more experienced negative time interference than female, on the other hand, in family${\rightarrow}$work spillover, female more experienced negative energy depletion than male. Second, there were significant differences in domains which work-family spillover occurs according to sex. Male more experienced negative work${\rightarrow}$family spillover than female in marital relations and parent-child relations domains while female more experienced negative family${\rightarrow}$work spillover than male in parent-child relations and home management domains. Third, there were significant differences between wife and husband in work-family spillover process. In work${\rightarrow}$family spillover, husband more experienced negative time interference than wife, on the other hand, in family${\rightarrow}$work spillover, wife more experienced negative energy depletion than husbands. Forth, there were significant differences between wife and husband in domains which work-family spillover occurs. Male more experienced negative work${\rightarrow}$family spillover than female in marital relations, parent-child relations, and leisure life domains. And while husband more experienced negative family${\rightarrow}$work spillover than wife in parent-child relations, wife more experienced negative family${\rightarrow}$work spillover than husband in home management domains.

• Asian-Australasian Journal of Animal Sciences
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• 제26권8호
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• pp.1144-1151
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• 2013

In this study, protein domains with cellulase activity in goat rumen microbes were investigated using metagenomic and bioinformatic analyses. After the complete genome of goat rumen microbes was obtained using a shotgun sequencing method, 217,892,109 pair reads were filtered, including only those with 70% identity, 100-bp matches, and thresholds below $E^{-10}$ using METAIDBA. These filtered contigs were assembled and annotated using blastN against the NCBI nucleotide database. As a result, a microbial community structure with 1431 species was analyzed, among which Prevotella ruminicola 23 bacteria and Butyrivibrio proteoclasticus B316 were the dominant groups. In parallel, 201 sequences related with cellulase activities (EC.3.2.1.4) were obtained through blast searches using the enzyme.dat file provided by the NCBI database. After translating the nucleotide sequence into a protein sequence using Interproscan, 28 protein domains with cellulase activity were identified using the HMMER package with threshold E values below $10^{-5}$. Cellulase activity protein domain profiling showed that the major protein domains such as lipase GDSL, cellulase, and Glyco hydro 10 were present in bacterial species with strong cellulase activities. Furthermore, correlation plots clearly displayed the strong positive correlation between some protein domain groups, which was indicative of microbial adaption in the goat rumen based on feeding habits. This is the first metagenomic analysis of cellulase activity protein domains using bioinformatics from the goat rumen.

• 한국진공학회:학술대회논문집
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• 한국진공학회 2013년도 제45회 하계 정기학술대회 초록집
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• pp.146.2-146.2
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• 2013

Copper is considered to be the most promising substrate for the growth of high-quality and large area graphene by chemical vapor deposition (CVD), in particular, on the (111) facet. Because the interactions between graphene and Cu substrates influence the orientation, quality, and properties of the synthesized graphene, we studied the interactions using angle-resolved photoemission spectroscopy. The evolution of both the Shockley surface state of the Cu(111) and the ${\pi}$ band of the graphene was measured from the initial stage of CVD growth to the formation of a monolayer. Graphene growth was initiated along the Cu(111) lattice, where the Dirac band crossed the Fermi energy (EF) at the K point without hybridization with the d-band of Cu. Then two rotated domains were additionally grown as the area covered with graphene became wider. The Dirac energy was about -0.4 eV and the energy of the Shockley surface state of Cu(111) shifted toward the EF by ~0.15 eV upon graphene formation. These results indicate weak interactions between graphene and Cu, and the electron transfer is limited to that between the Shockley surface state of Cu(111) and the ${\pi}$ band of graphene. This weak interaction and slight lattice mismatch between graphene and Cu resulted in the growth of rotated graphene domains ($9.6^{\circ}$ and $8.4^{\circ}$), which showed no significant differences in the Dirac band with respect to different orientations. These rotated graphene domains resulted in grain boundaries which would hinder a large-sized single monolayer growth on Cu substrates.

• 한국정보디스플레이학회:학술대회논문집
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• 한국정보디스플레이학회 2005년도 International Meeting on Information Displayvol.I
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• pp.357-360
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• 2005

A novel wide viewing angle flexible liquid crystal display will be demonstrated. The crossed matrix-type micro-cell structure was adopted in this design. The LC domains were divided into four different tilted directions by the combination effect of pixel fringe field and crossed matrix type micro-cell. It can create four domains without rubbing process and form the cell gap without spacer. For flexible color fabrication, a novel inkjet printing technology is adopted. This cost effective wide viewing angle color flexible LCD technology can be a good solution for high performance flexible LCD.

• Carbon letters
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• 제15권3호
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• pp.151-161
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• 2014

Exceptional progress has been made with chemical vapor deposition (CVD) of graphene in the past few years. Not only has good monolayer growth of graphene been achieved, but large-area synthesis of graphene sheets has been successful too. However, the polycrystalline nature of CVD graphene is hampering further progress as graphene property degrades due to presence of grain boundaries. This review will cover factors that affect nucleation of graphene and how other scientists sought to obtain large graphene domains. In addition, the limitation of the current research trend will be touched upon as well.

• 정신신체의학
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• 제2권1호
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• pp.46-51
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• 1994

Model' is one of the well-used, but poorly understood word in the neurobehavioral research. After Darwin's evolutionary theory, it has been generally believed that human is different from animals in terms of the complexity, not of the essential. This notion could be applied to the mind as well as body. Therefore, it became possible to establish animal models in the scientific field of mind. Experimental analysis of the animal behavior becomes an important area for establishing an animal model of human psychopathology because behavior is the ambassador of the mind. A model emphasizes a structural correspondence between sets of causally related variables in two different domains such as the animal and the human. The first selection of elements of the two domains in correspondence called the initial analogy. Once the initial analogy is formed. causally related variables in the two domains are examined and arrayed The structural parallel is the formal analogy of a model, and similarities between corresponding variables are called material analogy. Models may serve any of three major functions ; heuristic, evidential and representative. In many cases, utilizing models may be more practical than directly assessing the domain of primary interest, since technical and/or ethical problems are more serious in the human domain. Although modeling is important to study human psychopathology, rare animal models approved to be a good model for the human psychopathology up to now. Developing the appropriate model is urgent to solve many problems raised from human psychopathology.

• 한국콘텐츠학회논문지
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• 제16권1호
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• pp.13-23
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• 2016

연구는 유아교육기관의 창의적 역량에 대한 유아교사의 인식과 실행 간의 차이 및 유아교사의 배경변인에 따른 차이를 살펴보는데 목적이 있다. 연구 대상은 대구 경북 지역에 소재한 유치원에 근무하는 유아교사 144명이며, 유아교육기관의 창의적 역량의 영역별 하위내용 인식 및 실행에 관한 설문지를 통해 자료를 수집하였다. 수집된 자료는 SPSS 20.0 프로그램을 사용하여 대응표본 t-검증, 일원변량분석, Scheffe 사후검증을 통해 분석하였다. 연구결과 첫째, 유아교육기관의 창의적 역량의 영역별 내용에 대한 유아교사의 인식과 실행 간 차이는 다양성, 자율성, 리더십 영역의 모든 하위내용에서 유의한 차이가 있었다. 둘째, 유아교사의 배경변인에 따른 인식과 실행 간의 차이는 유아교사의 직급별 자율성 영역의 하위내용, 학력별 리더십 영역의 하위내용, 경력별 다양성 영역의 하위내용, 그리고 연령에 따른 다양성 영역과 리더십 영역의 하위내용에서 각각 유의한 차이가 나타났다.

• 대한지리학회지
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• 제38권4호
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• pp.518-534
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• 2003

선행연구 가운데에는 정보통신기술의 거리 극복 가능성을 중시하여 광범위한 분산과 지역 연계를 그려본 사례가 많으며, 도시간 수평적 연계를 내다보는 도시 네트워크 가설이 그 한가지이다. 이 연구는, 인터넷의 정보교류를 지표로 삼아 이 쟁점을 가리는 데 목적을 두어. 한국 도메인(.kr)의 대부분을 차지하는 상업. 학술. 비영리 도메인들의 분포와 도메인간 하이퍼링크를 분석하였다. 연구 결과, 도시 네트워크의 가설보다는 정보화로 말미암아 종주도시의 지배가 더욱 강화되고 있다고 판단할만한 증거들이 거듭 드러났다. 도메인과 하이퍼링크의 서올 집중 경향이 두드러져서, 서울이 전국에서 마치 하나의 고립된 섬처럼 정보의 공급원과 소비지의 역할을 하는 것으로 밝혀졌다. 다만 학술 도메인의 경우는, 전반적으로는 서울의 우산 아래 있기는 하지만. 지방 도시들 사이에 네트워크가 어느 정도 형성되고 있어 앞으로 더 면밀하게 검토해 볼 필요를 제기하였다.

• 대한화학회지
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• 제68권1호
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• pp.40-53
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• 2024

이 연구는 화학 핵심 개념, 화학 행동 영역, 수학 행동 영역으로 구성된 3차원 분석틀을 이용하여 2019학년도부터 2022학년도까지 4개년도의 대학수학능력시험 화학 I 문항을 분석하여 영역별 출제 문항 수와 비율을 알아보고 이를 바탕으로 각 영역 간의 관계에 대하여 알아보았다. 연구 결과, 대학수학능력시험 화학 I 문항은 핵심 개별 별로 비교적 고르게 출제되었으나, 고난도 문항은 양적 관계에 편중되었다. 화학 행동 차원의 경우, 문제 인식 및 가설 설정, 탐구 설계 및 수행 영역의 문항 출제 비율이 매우 낮았고, 고난도 문항의 경우 결론 도출 및 평가에 편중되었다. 수학 행동 영역의 경우, 발견적 추론 영역과 연역적 추론 영역에서 출제된 문항 수가 매우 적었고, 고난도 문항은 내적 문제해결력 영역에 편중되었다. 또한, 특정 화학 핵심 개념과 화학의 행동 영역은 특정 수학 행동 영역과 높은 연관성을 보였으며, 이러한 특징은 학생들이 어려워하는 고차원적 수학 행동 영역을 포함하는 문항에서 두드러지게 나타났다.