• Korean Journal of Animal Reproduction
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• v.12 no.1
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• pp.1-10
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• 1988

In order to investigate the spermiogenesis of the Korean Native Jin-do dog, the tissue sampled from the testis and the epididymis of matured dog were conventionally prepared as thin sections. And then these sections were observed under a transmission electron microscope. Based on the development of various permanent organelle and certain transient structures, the spermiogenesis of the dog could be divided into four major phases: Golgi, cap, acrosome and maturation; and these phases had been futher subdivided into seven stages. In the maturatin phase, the karyoplasm has been condensed completely and the nucleus eleongated as the movement of the unclear ring. The equatorial segment has notable waist-form at the late acrosomal stage. In the cross section of the tail, it shows a typicl 9＋9＋2 pattern of the axial fiber bundle condisting of the dense fibers, the inner fibers, and a central pair. The dense fiber number 1, 5, 6, and 9 were larger in diameter than the rest of the dense fibers.

• Korean Journal of Animal Reproduction
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• v.13 no.1
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• pp.49-55
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• 1989

The Results of colchicine and pretreatment time have shown that the cells of Coho Salmon(Oncorhynchus Kisutch) were effectively injected 6 hours prior to the harvest at a final concentration of 10$\mu\textrm{g}$/g body weight. The cell-density from 8,000 cells/㎣ (=8${\times}$106 ml) was found as ideal. The best effect of hypotonic solution in proportion to the cell is 20 : 1 and 50 minutes. The model number of diploid chromosome in this species was 2n=60. The karyotype analysis proved that the diploid complement consisted of 19 pairs of metacentric-, 4 pairs of submetacentric- and 7 paris of acrocentric-chromosomes. The diagrammatic representation proved that the diploid complement consisted of 7 pairs of metacentric-, 2 pairs of submetacentric- and 1 pair of acrocentric-chromosomes.

• Korean Journal of Animal Reproduction
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• v.12 no.3
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• pp.167-173
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• 1988

The present experiment was carried out to investigate super-ovulating response, ovulation time and fertilizability in the angora rabbit that gonadotropin was treated. The results obtained were summarized as follows ; 1. Number of ovulating point and ovarian weight in PMSG 100I.U.(25${\pm}$2.45;1104.2${\pm}$110.6mg) and PMSG 200I.U.(30.6${\pm}$1.76, 1330.0${\pm}$153.9mg) treated group were significantly higher than natural mating(6.6${\pm}$1.49, 560.2${\pm}$60.6mg) and HCG treated group(9.8${\pm}$0.8;651.6${\pm}$55.1mg)(P<0.01). 2. Survival rate and recovery rate in natural mating, HCG and PMSG 100I.U. treated group were significantly higher than PMSG 200I.U. treated group.(P<0.01)(P<0.05). 3. Ovulation started at 10hrs and mostly finished at 16hrs after HCG injection. 4. The fertilizable life of egg ovulated was during 8hrs after ovulation.

• Korean Journal of Animal Reproduction
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• v.11 no.2
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• pp.122-126
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• 1987

This study was carried out to investigate the effects of the glycerol equilibration methods and embryo grades before freezing on the survival rate after thawing in mouse embryos. The results obtained from this study were as follows: 1. The number of embryos of grade I(Excellent), II(Good) and III(Fair) before freezing in this study was 97(27.4%), 160(45.2%) and 97(27.4%), respectively. 2. The average survival rate of frozen-thawed embryos in 3 and 5 steps glycerol equilibration was 66.7% and 64.1%, and the rate of transfererable embryos after culture was 68.9% and 69.0%, respectively. 3. Out of embryo grade I and II before freezing, the transferable rate after thawing was 75.2% and 48.1%, respectively, and grade I embryos before freezing was higher transferable rate than that of grade II.

• Korean Journal of Animal Reproduction
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• v.11 no.2
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• pp.127-131
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• 1987

This study was carried out to obtain the basic information on splitting and culture of mouse and bovine embryos. Two-, four-, eight-, cell and morula mouse embryos were digested with pronase, splitted in vitro by micro-glass needel with hand, and bovine embryos were splitted by micromanipulator. The splitted embryos were cultured under 5% of CO2 gas in air at 37$^{\circ}C$ for 48-72 hours. The results obtained in this study were summarized as follows: 1. The mouse and cattle were superovulated by 5IU of PMS and HCG, and 2500IU of PMS and 25mg of PGF2$\alpha$, respectively. The average number of embryos after superovulation were 32.5$\pm$8.2 and 7.5$\pm$3:1, respectively. 2. Out of total 122 embryos splitted, the successful splitting rate was 75.0%, 66.7%, 68.4% and 71.4% in 2-, 4-, 8- and morula embryos in mouse, respectively. There was no different splitting rate between mouse(71.4%) and bovine embryos(66.7%) in morula. 3. The successful culture rate of splitted embryos was 68.0% and 67.9% in mouse and bovine embryos, respectively.

• Korean Journal of Animal Reproduction
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• v.17 no.1
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• pp.13-19
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• 1993

We investigated the effects of CP-2 extracted from the mixture of Copis and Croton tiglium L, which showed very high cytotoxic effect to the various tumor cells, on the growth and steroidenesis of primary and transformed cell lines PA-GS6 and PO-GRS1 by cotransfectionwith SV40 and Ha ras oncogenes. CP-2 inhibited the growth of PA-GS6 and PO-GRS1 in a dose dependent manner when the growth of them was measured by cell number and by protein content, while CP-2 did not affect the growth of primary granulose cells. Productions of progesterone ofprimary and transformed granulosa cells were stimulated by forskolin, but this stimulatory effect was blocked by treatment of CP-2. Clinical application of CP-2 asa new anti-cancer drug and utilization of transformed granulosa cells as a model system for the screening of anti-cancer drug were also discussed.

• Korean Journal of Animal Reproduction
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• v.6 no.1
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• pp.36-38
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• 1982

For the purpose of identifing the reationship between the first egg day and other economic traits; egg production and egg weight, this experiment was divided 200 birds into four first egg day groups and observed egg number and egg weight every day during 11 months from 2nd December, 1979 to 31st October, 1980 at Animal farm, Yeungnam University. Birds showing 148∼155day in first egg day layed the most eggs among four groups and they produced not only 1.5g heavier eggs than the earlist first egg day group but also similar eggs compared with later matured birds. In egg production above 55g, 148∼155day group showed the best record as a 83.72% of total eggs and also the most egg mass during 11 months.

• Journal of Animal Reproduction and Biotechnology
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• v.36 no.4
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• pp.323-326
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• 2021

A cat who is a 15-year-old and spayed female visited an animal clinic with severe coughing symptoms. Since the cat's coughing symptoms had worsened from the age of 10 and X-rays showed a bronchial pattern in the lungs, it was diagnosed as Chronic Obstructive Pulmonary Disease (COPD). She received three injections of stem cells isolated from the amniotic membrane on days 0, 7, and 23. Although there was no improvement in the clinical findings on the x-ray, the number of coughing was significantly reduced. In addition, even after long-term follow-up post treatment for a month, she was stable with almost no coughing.

• Asian-Australasian Journal of Animal Sciences
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• v.11 no.5
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• pp.491-497
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• 1998

Four types of serum supplements viz. estrus cow serum (ECS), estrus buffalo serum (EBS), pro-estrus buffalo serum (PrBS) and post-estrus buffalo serum (PtBS), added to TCM-199, were evaluated for in vitro maturation and fertilization of buffalo follicular oocytes. The oocytes were recovered from buffalo ovaries after slaughter, using either aspiration or scoring (multiple incisions) method. The recovered oocytes were categorized as A, B and C based on their cumulus investment and ooplasm homogeneity and cultured in four media. The in vitro matured oocytes were inseminated with $1{\times}10^6$ spermatozoa washed in 2.9% sodium citrate solution. The scoring method yielded greater number of morphologically good oocytes than the aspiration method (3.85 vs 1.76 per ovary, p < 0.01). The maturation rates of three categories of oocytes did not differ from one another. The maturation rates of 80.00, 82.08, 78.77 and 66.23%, while the fertilization rates of 54.54, 55.38, 52.80 and 36.76% were recorded for media containing ECS, EBS, PrBS, and PtBS, respectively. The medium containing PtBS gave lower maturation, as well as fertilization, rates than the other three media (p < 0.05). Thus, the scoring method was better than the aspiration method for the recovery of follicular oocytes. The oocytes categorized A, B and C had similar maturation capabilities. The TCM-199 containing buffalo/cow serum collected at pro-estrus or estrus appeared better for in vitro maturation and fertilization of buffalo follicular oocytes than that containing serum collected at post estrus.

• Journal of the Institute of Electronics Engineers of Korea SP
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• v.39 no.3
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• pp.1-10
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• 2002

Because of the reduced number of displayable gray levels resulting from inverse gamma correction, images on a plasma display panel (PDP) exhibit undesirable false contours in dark areas. An error diffusion method has been applied to remedy this problem. However, it is computationally expensive and requires large memory resources. This paper proposes a computationally efficient dithering based technique to improve the gray level reproduction capability in dark areas. In the proposed method, multiple dithering masks ate utilized in turn to improve the gray level reproduction in dark areas. Also, in order to reduce undesirable regular patterns generated by the dithering method, positions of threshold values within a given dithering mask are changed. Compared to the error diffusion method, the proposed method requires much less computations and memory resources with a comparable gray level reproduction capability.