• New & Renewable Energy
• /
• v.19 no.4
• /
• pp.46-52
• /
• 2023

Methane is the second most emitted greenhouse gas after carbon dioxide. Despite lower emissions than those of carbon dioxide, methane receives significant attention owing to its more than 20-fold higher global warming potential. Consequently, the importance of research on methanotrophic bacteria, microorganisms capable of converting methane gas into high-value materials, is increasingly emphasized. In the case of methanotrophic bacteria, knowledge on episomal plasmids that can be used for genetic engineering remains lacking, which poses significant challenges to the engineering process. The replication origin sequences of natural plasmids within methanotrophic bacteria have been predicted through in silico methods. The basic characteristics of the replication origin, such as a high A/T ratio, repetitive sequences, and proximity to proteins related to replication, have been used as criteria for identifying the replication origin. As a result, a region with a sequence of 18 base pairs repeated eight times could be identified. The putative replication origin sequence thus identified generally takes the form of iterons, but it also possesses unique features such as the length of the gap between iterons and the repetition of identical iteron sequences. This information can be valuable for future design of episomal plasmids applicable to methanotrophs.

• Journal of Communications and Networks
• /
• v.16 no.4
• /
• pp.378-384
• /
• 2014

Replication routing can greatly improve the data delivery performance by enabling multiple replicas of the same packet to be transmitted towards its destination simultaneously. It has been studied extensively recently and is now a widely accepted routing paradigm in delay tolerant networks (DTNs). However, in this field, the issue of how to maximize the utilization efficiency of limited replication quota in a resource-saving manner and therefore making replication routing to be more efficient in networks with limited resources has not received enough attention. In this paper, we propose a DTN routing protocol with back-pressure based replica distribution. Our protocol models the replica distribution problem from a resource allocation perspective and it utilizes the idea of back-pressure algorithm, which can be used for providing efficient network resource allocation for replication quota assignment among encountered nodes. Simulation results demonstrate that the proposed protocol significantly outperforms existing replication routing protocols in terms of packet delay and delivery ratio.

• Transactions of Materials Processing
• /
• v.21 no.2
• /
• pp.126-130
• /
• 2012

Accurate optical texturing of light guide plate over the entire surface area is an important technical issue in LCD TV industry. Injection molding process has the potential to produce large light guide plates having highly efficient optical textures such as micro-prism array. This study is focused on the effect of the degree of replication of the micro texture of the 40" injection molded light guide plates on the overall optical performance of the display panel. Measured replication ratios of the micro-textures formed with three different types of injection molding process were considered in the modeling of prismatic micro segment array. Optical simulation was conducted and results were discussed.

• 한국정보디스플레이학회:학술대회논문집
• /
• 2007.08a
• /
• pp.313-315
• /
• 2007

3M has developed a Precision Replication Process for the production of PDP Barrier Ribs and other display devices. This process, is fast, clean and capable of producing very high resolution (FHD and beyond), high aperture ratio and fine cell structures. Recent advances in materials and process features will be discussed.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.30 no.7 s.250
• /
• pp.773-778
• /
• 2006

High-aspect-ratio nano-hair or nano-pillar arrays have great potential in a variety of applications. In this study, we present a simple and cost-effective replication method of high-aspect-ratio polymer nano-hair arrays. Highly ordered nano-porous AAO (anodic aluminum oxide) template was utilized as a reusable nano-mold insert. The AAO nano-mold insert fabricated by the two-step anodization process in this study had close- packed straight nano-pores, which enabled us to replicate densely arranged nano-hairs. The diameter, depth and pore spacing of the nano-pores in the fabricated AAO nano-mold insert were about 200nm, $1{\mu}m$ and 450nm, respectively. For the replication of polymer nano-hair arrays, a UV nano embossing process was applied as a mass production method. The UV nano embossing machine was developed by our group for the purpose of replicating nano-structures by means of non-transparent nano-mold inserts. Densely arranged high-aspect-ratio nano-hair arrays have been successfully manufactured by means of the UV nano embossing process with the AAO nano-mold insert under the optimum processing condition.

• Proceedings of the KSME Conference
• /
• 2003.04a
• /
• pp.945-949
• /
• 2003

LIGA(Lithographie Galvanoformung Abformung), a fabrication method for the production of microstructrues with a high aspect ratio, is now playing an important role in a fabrication of polymeric optical waveguide device as the replication processes have been developed such as hot embossing and injection molding. The present report deals with the fabrication of Ni master used for the replication of multi-mode polymeric optical waveguide. With the basic technological features in the sequence of the LIGA technique, we fabricated Ni master with 12 channel microstructures of $100\;{\times}\;100{\mu}m\;^2{\times}\;60mm$, and achieved an accuracy of ${\pm}1\;{\mu}m$. Manufactured polymeric optical wavegude with the same using hot embossing process has also the same accuracy and approved its mass production capability.

• Transactions of Materials Processing
• /
• v.20 no.1
• /
• pp.23-28
• /
• 2011

For the fabrication of nano patterned products manufacturing a nano patterned mold is needed in advance. The nano patterned stamper was fabricated by electroforming the AAO master with nickel. The surface of nickel-plated stamper had nano-patterned holes with the diameter of 73 nm and the depth of 83 nm. Hot embossing was used for forming P3HT sheet and the process factors of hot embossing were closer as pressure, temperature and time. In the present paper hot embossing experiments were performed to find the main process conditions to affect the replication ratio of nano patterns on surface of P3HT sheet. As a result, main contributing factors for the replication ratio of hot embossed pattern could be sequentially enumerated as pressure, temperature and time.

• Transactions of the Korean Society of Mechanical Engineers A
• /
• v.38 no.1
• /
• pp.51-57
• /
• 2014

Ultrasonic imprinting is a novel process for replicating micropatterns on thermoplastic polymer substrates with low energy consumption and short cycle time. The polymer substrate is softened by the frictional heat and repetitive deformation energy under ultrasonic excitation; thus, a number of micropatterns are replicated on the softened polymer substrate. In the present work, the effect of mold temperature on the replication characteristics of ultrasonic imprinting is investigated. The temperature change in the patterned region is measured by varying the mold temperature. Numerical simulation is then performed for investigating pattern replication characteristics under various mold temperatures. In addition, pattern replication ratio and uniformity are compared through various experimental measurements. Through the results of these comparisons, it is found that the mold temperature has a significant positive effect on the replication characteristics of ultrasonic imprinting.

• Korean Journal of Materials Research
• /
• v.13 no.1
• /
• pp.6-10
• /
• 2003

Electroplated Ni and Ni-W micro-molds using LIGA-like process for replication of micro-components such as microfluidic parts and micro optical parts have been investigated. In general, it is hard to produce micro-parts using conventional mechanical processes. Micro-mold formed by LIGA-like process could fabricate micro-parts with high aspect ratio. In this paper, fabrication and properties of electroplated Ni molds with varying applied current types as well as those of Ni-W molds were investigated. Ni molds fabricated under pulse-reverse current showed the highest hardness value of about 160 Hv. Ni-W molds showed the hardness of about 500 Hv which was much harder than that of Ni electroplated molds. The above results suggested that high quality micro-molds could be fabricated by using Ni electroplating of pulse-reverse type for core molds and sequential Ni-W alloys coating.

• Proceedings of the Korean Society of Precision Engineering Conference
• /
• 2008.06a
• /
• pp.585-586
• /
• 2008