• 대한바이러스학회지
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• 제29권4호
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• pp.221-233
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• 1999

Prospect Hill Virus (PHV) is the well known serotype of hantavirus, a newly established genus in family Bunyaviridae. Extensive studies have upheld the original view of PHV genetics with three genes such as nucleocapsid (N) protein, envelope proteins (G1, G2) and RNA dependent RNA polymerase. In this study, we report the existence of additional gene that is encoded in an overlapping reading frame of the N protein gene within S genome segment of PHV. This gene is expected to encode a nonstructural small (NSs) protein and it seems to be only found in PHV infected cell. The presence and synthesis of NSs protein could be demonstrated in the cell infected with PHV using anti-peptide sera specific to the predicted amino acid sequence deduced from the second open reading frame. Ribosomal synthesis of this protein appears to occur at AUG codon at the 83rd base of S genome segment, downstream of N protein initiation codon. This protein is small in size (10.4 KDa) and highly basic in nature. The expression strategy of NSs protein appears that a signal mRNA is used to translate both N and NSs protein in PHV infected cell. 10 KDa protein in virus infected cell lysates can bind to mimic dsRNA. This fact strongly suggests that NSs protein may be involved in virus replication on late phase of viral life cycle.

• Genomics & Informatics
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• 제14권3호
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• pp.104-111
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• 2016

Zika virus (ZIKV) is a mosquito borne pathogen, belongs to Flaviviridae family having a positive-sense single-stranded RNA genome, currently known for causing large epidemics in Brazil. Its infection can cause microcephaly, a serious birth defect during pregnancy. The recent outbreak of ZIKV in February 2016 in Brazil realized it as a major health risk, demands an enhanced surveillance and a need to develop novel drugs against ZIKV. Amodiaquine, prochlorperazine, quinacrine, and berberine are few promising drugs approved by Food and Drug Administration against dengue virus which also belong to Flaviviridae family. In this study, we performed molecular docking analysis of these drugs against nonstructural 3 (NS3) protein of ZIKV. The protease activity of NS3 is necessary for viral replication and its prohibition could be considered as a strategy for treatment of ZIKV infection. Amongst these four drugs, berberine has shown highest binding affinity of -5.8 kcal/mol and it is binding around the active site region of the receptor. Based on the properties of berberine, more similar compounds were retrieved from ZINC database and a structure-based virtual screening was carried out by AutoDock Vina in PyRx 0.8. Best 10 novel drug-like compounds were identified and amongst them ZINC53047591 (2-(benzylsulfanyl)-3-cyclohexyl-3H-spiro[benzo[h]quinazoline-5,1'-cyclopentan]-4(6H)-one) was found to interact with NS3 protein with binding energy of -7.1 kcal/mol and formed H-bonds with Ser135 and Asn152 amino acid residues. Observations made in this study may extend an assuring platform for developing anti-viral competitive inhibitors against ZIKV infection.

• Journal of Microbiology and Biotechnology
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• 제31권4호
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• pp.510-519
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• 2021

The pathological impact of haze upon the phyllosphere microbiota awaits investigation. A moderate degree of haze environment and a clean control were selected in Chengdu, China. Artemisia argyi, a ubiquitously distributed and extensively applied Chinese herb, was also chosen for experiment. Total genome DNA was extracted from leaf samples, and for metagenome sequencing, an Illumina HiSeq 2500 platform was applied. The results showed that the gene numbers of phyllosphere microbiota derived from haze leaves were lower than those of the clean control. The phyllosphere microbiota derived from both haze and clean groups shared the same top ten phyla; the abundances of Proteobacteria, Actinomycetes and Anorthococcuso of the haze group were substantially increased, while Ascomycetes and Basidiomycetes decreased. At the genus level, the abundances of Nocardia, Paracoccus, Marmoricola and Knoelia from haze leaves were markedly increased, while the yeasts were statistically decreased. KEGG retrieval demonstrated that the functional genes were most annotated to metabolism. An interesting find of this work is that the phyllosphere microbiota responsible for the synthesis of primary and secondary metabolites in A. argyi were significantly increased under a haze environment. Relatively enriched genes annotated by eggNOG belong to replication, recombination and repair, and genes classified into the glycoside hydrolase and glycosyltransferase enzymes were significantly increased. In summary, we found that both structure and function of phyllosphere microbiota are globally impacted by haze, while primary and secondary metabolites responsible for haze tolerance were considerably increased. These results suggest an adaptive strategy of plants for tolerating and confronting haze damage.

• Journal of Plant Biotechnology
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• 제48권4호
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• pp.289-303
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• 2021

Covid-19 is an ongoing pandemic as we speak in 2022. This infectious disease is caused by the SARS-CoV-2 virus, which infects cells by binding to the angiotensin-converting enzyme 2 (ACE2) receptor on the cell surface. Thus, strategies that inhibit the binding of SARS-CoV-2 to the ACE2 receptor can stop this contagion. Hanjeli (Coix lacryma-jobi) essential oil contains many bioactive compounds, including dodecanoic acid; tetradecanoic acid; 7-Amino-8-imino-2-(2-imino-2H-chromen-3-yl); and 1,5,7,10-tetraaza-phen-9-one. These compounds suppress viral replication and may prevent Covid-19. Accordingly, this study assessed whether, these four limonoid compounds can block the ACE2 receptor. To this end, their physicochemical properties were predicted using Lipinski's "rule of five" on the SwissADME website, and their toxicity was assessed using the online tools ProTox and pkCSM. Additionally, their interactions with the ACE2 receptor were predicted via molecular docking using Autodock Vina. All the four compounds satisfied the "rule of five" and tetradecanoic acid was predicted to have a higher affinity than the comparison compound remdesivir and the original ligand of ACE2. Molecular docking results suggested that the compounds from hanjeli essential oil interact with the active site of the ACE2 receptor similarly as the original ligand and remdesivir. In conclusion, hanjeli essential oil contains compounds predicted hinder the interaction of SARS-CoV-2 with the ACE2 receptor. Accordingly, our data may facilitate the development of a phytomedical strategy against SARS-CoV-2 infection.

• Journal of Veterinary Science
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• 제24권1호
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• pp.15.1-15.13
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• 2023

Background: Inactivated vaccines are limited in preventing foot-and-mouth disease (FMD) due to safety problems. Recombinant virus-like particles (VLPs) are an excellent candidate for a novel vaccine for preventing FMD, given that VLPs have similar immunogenicity as natural viruses and are replication- and infection-incompetent. Objectives: The 3C protease and P1 polyprotein of type O FMD virus (FDMV) was expressed in yeast Hansenula polymorpha to generate self-resembling VLPs, and the potential of recombinant VLPs as an FMD vaccine was evaluated. Methods: BALB/c mice were immunized with recombinant purified VLPs using CpG oligodeoxynucleotide and aluminum hydroxide gel as an adjuvant. Cytokines and lymphocytes from serum and spleen were analyzed by enzyme-linked immunosorbent assay, enzyme-linked immunospot assay, and flow cytometry. Results: The VLPs of FMD were purified successfully from yeast protein with a diameter of approximately 25 nm. The immunization of mice showed that animals produced high levels of FMDV antibodies and a higher level of antibodies for a longer time. In addition, higher levels of interferon-γ and CD4⁺ T cells were observed in mice immunized with VLPs. Conclusions: The expression of VLPs of FMD in H. polymorpha provides a novel strategy for the generation of the FMDV vaccine.

• IMMUNE NETWORK
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• 제21권1호
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• pp.8.1-8.17
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• 2021

The global crisis caused by the coronavirus disease 2019 (COVID-19) led to the most significant economic loss and human deaths after World War II. The pathogen causing this disease is a novel virus called the severe acute respiratory syndrome coronavirus 2 (SARSCoV-2). As of December 2020, there have been 80.2 million confirmed patients, and the mortality rate is known as 2.16% globally. A strategy to protect a host from SARS-CoV-2 is by suppressing intracellular viral replication or preventing viral entry. We focused on the spike glycoprotein that is responsible for the entry of SARS-CoV-2 into the host cell. Recently, the US Food and Drug Administration/EU Medicines Agency authorized a vaccine and antibody to treat COVID-19 patients by emergency use approval in the absence of long-term clinical trials. Both commercial and academic efforts to develop preventive and therapeutic agents continue all over the world. In this review, we present a perspective on current reports about the spike glycoprotein of SARS-CoV-2 as a therapeutic target.

• 생명과학회지
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• 제28권9호
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• pp.1007-1015
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• 2018

E6AP (E6-associated protein)는 C형 간염바이러스(hepatitis C virus, HCV)의 코어 단백질 유비퀴틴화와 프로테오좀 분해를 유도하여 캡시드 조립을 저해함으로써 HCV 복제를 억제하는 것으로 알려져 있다. 반면에 HCV 코어 단백질은 숙주의 항바이러스 방어계에 대항하고 자신의 유비퀴틴-의존적 프로테아좀 분해를 막기 위하여 DNA 메틸화를 통하여 E6AP 발현을 저해하는 전략을 진화과정에서 획득하였다. 본 연구에서는 HCV 코어 단백질이 E6AP 발현을 저해하는 기전을 밝혀내고자 하였다. HCV 코어 단백질은 HepG2 세포에서 DNA 메틸화 효소들인 DNMT1, 3a 및 3b의 단백질 수준과 효소 활성을 증가시켜 프로모터 과메틸화를 통하여 E6AP 발현을 저해하였지만 p53를 발현하지 않는 Hep3B 세포에서는 이러한 효과들이 관찰되지 않았다. 흥미롭게도 Hep3B 세포에 p53만 과발현시키면 HCV 코어 단백질이 없더라도 DNMT가 활성화되고 프로모터 과메틸화를 통하여 E6AP 발현이 저해되었다. 또한 p53 녹다운 및 과발현 실험을 통하여 p53 활성화가 HCV 코어 단백질의 효과에 필수적임을 알 수 있었다. 이로 인하여 Hep3B 보다 HepG2 세포에서 낮은 수준의 유비퀴틴화된 HCV 코어 단백질이 검출되었다. 따라서 HCV 코어 단백질은 p53-의존적으로 자신의 유비퀴틴-매개성 프로테아좀 분해를 저해한다.

• BMB Reports
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• 제42권1호
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• pp.59-64
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• 2009

Hepatitis B virus (HBV) infection is highly prevalent worldwide. The major challenge for current antiviral treatment is the elevated drug resistance that occurs via rapid viral mutagenesis. In this study, we developed AAV vectors to simultaneously deliver two or three shRNAs targeting different HBV-related genes. These vectors showed markedly better antiviral effects than ones that delivered a single shRNA in vitro. A dual shRNA expression vector (AAV-157i/1694i), which simultaneously expressed two shRNAs targeted the S and X genes of HBV, reduced HBsAg, HBeAg and HBV DNA levels by $87{\pm}4$, $80.3{\pm}2.6$ and $86.2{\pm}7%$ respectively, eight days post-transduction. In a mouse model of prophylactic treatment, HBsAg and HBeAg were reduced to undetectable levels and the serum HBV DNA level was reduced by at least 100 fold. These results indicate that AAV-157i/1694i generates potent anti-HBV effects and that the strategy of constructing multi-shRNA expression vectors may lead to enhanced anti-HBV efficacy and overcome the evading mechanism of the virus and thus the development of drug resistance.

• 한국사회복지학
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• 제52권
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• pp.89-115
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• 2003

그동안 사회복지 임상실천 분야에서 주로 관심을 기울여 온 것은 개입방법의 개발과 효과성 측정 연구였다. 반면 이러한 연구 결과물이 어떻게 현장에 보급되고 활용되는지에 대한 관심은 상대적으로 부족한 편이었다. 이러한 현상은 결과적으로 연구와 실천간의 괴리를 초래할 수 있다는 비판이 제기되면서, 최근 외국에서는 사회복지 분야에서도 이론과 실천, 또는 학문과 현장을 잇는 중요한 기제로서 지식 및 기술의 보급과 활용에 대한 관심이 부각되고 있다. 이에 본 연구에서는 본 연구자가 프로그램 개발에 이어 현장보급 노력을 기울여 온 성장기술 프로그램을 중심으로 현장보급 과정과 활용현황을 살펴보고자 하였다. 이를 위하여 보급과 활용에 관한 기존 이론 및 모델들을 정리하고, 그 중 Herie와 Martin의 연구-실천 가교 모델에 의거하여 성장기술 프로그램의 현장 보급과정 및 활용 상황을 추적 조사하고 분석해 보았다. 그 결과 성장기술 프로그램 보급과정의 문제점으로는 구체적인 전략의 부재, 계획성 부족으로 인한 즉흥적인 대응, 초기 보급 대상자에 대한 허술한 파악 등을 도출할 수 있었다. 그리고 효과적인 보급과 활용을 위해서는 적절한 모델을 선택하고 그에 따라 보급과정을 적용하고 실천할 것, 프로그램 개발과정부터 보급에 대해 염두를 두고 진행할 것, 프로그램 개발 뿐 아니라 현장 피드백 확보를 위한 노력을 기울일 것, 그리고 사회복지 교육 및 연구에서 전파과정에 대한 부분을 강화할 것 등을 제언하였다. 이를 통해 몇 가지 한계점들에도 불구하고 보급 및 활용에 대한 중요성을 제시할 수 있었으며, 성장기술 프로그램의 현장 활용성도 파악할 수 있었다. 이와 같은 보급과 활용에 대한 논의들은 국내에서 진행되는 연구의 전파 뿐 아니라 그동안 외국으로부터 도입해온 개입방법들을 한국 상황에 맞게 토착화하기 위하여 앞으로 더욱 활성화될 필요가 있을 것이다.

• 인터넷정보학회논문지
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• 제17권6호
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• pp.17-23
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• 2016

사물인터넷 (IoT)과 같은 미래 망에서, 컴퓨팅 기기의 수는 기하급수적으로 증가할 것으로 예상되고, 각 사물들은 서로 통신하고 스스로 정보를 획득한다. 사물 인터넷 응용에 대한 관심 증가로 사물통신 (M2M)과 같은 기회적 애드혹 망에서 데이터를 전달하는 방송은 중요한 기술이다. 그리고 IoT를 위한 분산 망에서, 노드들의 에너지 효율성은 망 성능에서 중요한 요인이다. 이 논문에서, 우리는 전송 노드의 에너지 충전률, 사본 밀도 비율 그리고 송 수신 노드간의 거리률에 기초한 퍼지 논리에 따라 확률적으로 데이터를 전파하는 퍼지 논리 기반 멀티홉 방송 알고리즘 FPMCAST를 제안한다. 제안하는 FPMCAST에서, 추론 엔진은 입 출력 매개변수를 입 출력 소속 함수로 사상하는 27개의 if-then 규칙들로 구성된 퍼지 규칙 베이스에 기초한다. 퍼지 시스템의 출력은 재방송 확률에 대한 퍼지 집합을 정의하고, 그 퍼지 집합으로부터 수치 결과를 추출하기 위하여 비 퍼지화가 사용된다. 여기서 퍼지 집합을 비 퍼지화하기 위하여 무게중심법이 사용된다. 그리고 모의실험을 통하여 제안하는 FPMCAST의 성능을 평가한다. 모의실험으로부터, 우리는 제안하는 FPMCAST 알고리즘이 플러딩 알고리즘과 가시핑 알고리즘 보다 우수함을 입증하였다. 특히, FPMCAST 알고리즘은 각 노드의 잔여 에너지를 균등하게 소비하기 때문에 더 긴 망 수명을 갖는다.