• Proceedings of the Botanical Society of Korea Conference
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• 1999.07a
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• pp.17-20
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• 1999

In rice, limited efforts have been made to identify genes by the use of insertional mutagens, especially heterologous transposons such as the maize Ac/Ds. We constructed Ac and gene trap Ds vectors and introduced them into the rice genome by Agrobacterium-mediated transformation. In this report, rice plants that contained single and simple insertions of T-DNA were analyzed in order to evaluate the gene-tagging efficiency. The 3'end of Ds was examined for putative splicing donor sites. As observed in maize, three splice donor sites were identified at the 3'end of the Ds in rice. Nearly 80% of Ds elements wered excised from the original T-DNA sites, when Ac cDNA was expressed under a CaMV 35S promoter. Repetitive ratoon culturing was performed to induce new transpositions of Ds in new plants derived from cuttings. About 30% of the plants carried at least one Ds that underwent secondary transposition in the later cultures. 8% of transposed Ds elements expressed GUS in various tissues of rice panicles. With cloned DNA adjacent to Ds, the genomic complexities of the insertion sites were examined by Southern hybridization. Half of the Ds insertion sites showed simple hybriodization patterns which could be easily utilized to locate the Ds. Our data demonstrate that the Ac/Ds mediated gene trap system could prove an excellent tool for the analysis of functions of genes in rice. We discuss genetic strategies that could be employed in a largee scale mutagenesis using a heterologous Ac/Ds family in rice.

• Journal of The Korean Association of Information Education
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• v.23 no.6
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• pp.621-628
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• 2019

The 2015 revised curriculum offers elementary students an unplugged computing education as a way to learn the concepts and principles of computer science in an easy and fun way and to improve their computational thinking. Yutnori is a traditional board game unique to Korea, which contains various contents such as history, culture, and science of our people, which helps to cultivate cultural identity of learners, and can effectively promote cooperation and communication among members. In this paper, we examined the possibility that Yutnori could be used as an unplugged computing tool in elementary school software education and convergence education. Korean traditional board game Yutnori has elements that can learn the sun and movements of heavenly bodies as well as sequential, selection, and repetitive algorithm elements. Unplugged activities that apply Korean traditional culture Yutnori is expected to help foster creative convergence talents by improving elementary school students' computational thinking, communication and community.

• Journal of KIBIM
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• v.12 no.1
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• pp.42-54
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• 2022

BIM facilitates data transparency and consistency through three-dimensional parametric modeling and promotes the accurate managing and sharing of project information. In Korea, however, BIM-based detailed design of architectural interior finishes required during the Construction Documents phase increases the burden on architectural firms due to frequent design changes and manual workload. Therefore, the purpose of this study was to establish rule-based modules using parametric modeling that automates repetitive tasks that occur during the detailed design of interior finishing. Interviews with practitioners were conducted to analyze the major finishing elements. Of these floors, walls, and ceilings, which were the most rudimentary and common items, were selected as the subjects of the study. The modules developed in this study have two functions. One is to create new finish types, and the other is the automatic modeling of new types into rooms. For these functions, parameters that belonged to each finish and room element in a BIM model were analyzed and valid parameters directly used for parametric modeling were derived. Then, based on these parameters, rule-based modules for three elements, I.e., floors, walls, and ceilings were constructed with Revit Dynamo, and the effectiveness of the modules was verified with a pilot test. In conclusion, this study suggested a series of processes for automatic finishing to improve the efficiency of BIM-based architectural detailed design of finishes and to contribute in solving the chronic problems occuring during current design processes.

• Journal of Korean Society of Steel Construction
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• v.22 no.5
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• pp.411-420
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• 2010

In general, moving vehicles generate continuous and repetitive strain of energy on bridges. The strain energy can convert to electric energy due to its piezoelectric element. However, some factors should be considered in order to reasonably assess the feasibility such as load distribution applied on bridges and the relationship of strains generated according to loads. This study was carried out to estimate the generated voltage when piezoelectric elements were installed to a bridge. A steel-concrete composite specimen was fabricated and loads were applied, considering vehicle load-effects. As a result, the voltage generated in the element was evaluated and compared with the analysis equation of the piezoelectric effect.

• Journal of fish pathology
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• v.24 no.3
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• pp.297-305
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• 2011

A trap identification system for isolating functional sequences to allow the constitutive expression of foreign protein from Edwardsiella tarda was developed. Using the green fluorescent protein (GFP) reporter-based trap system, various functional sequences to drive heterologous expression of the GFP were selectable in Escherichia coli host. However from the bioinformatic sequence analysis, all the segments predicted as regulatory regions were not native promoters actually existing upstream of endogenous E. tarda genes. Instead, a number of non-authentic sequences, possibly resulted from the random shuffling and/or intermolecular ligation were also proven to be able to display a potent GFP expression in the recombinant E. coli. Further analysis with selected clones showed that both authentic and non-authentic sequences could function in as a constitutive promoter, leading quite a consistent and stable GFP expression after repetitive subcultures. Microscopic examination also confirmed the uniform pattern of GFP expression in every host bacterium. Semi-quantitative assay of GFP showed that there was no clear relationship between expression levels and organizational features of the promoters trapped. Functional promoter-like elements achieved in the present study could be a good starting material for multivalent genetic engineering of E. tarda in order to produce recombinant vaccines in a cost-effective fashion.

• Journal of Environmental Health Sciences
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• v.35 no.5
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• pp.343-354
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• 2009

Since Barker found associations between low birth weight and several chronic diseases later in life, the hypothesis of fetal origins of adult disease (aka, Barker Hypothesis) and epigenetics have been emerging as a new paradigm for geneenvironment interaction of chronic disease. Epigenetics is the study of heritable changes in gene silencing that occur without any change in DNA sequence. Gene expression can be regulated by several epigenetic mechanisms, including DNA methylation and histone modifications, which may be associated with chronic conditions, such as cancers, cardiovascular disease, and type-2 diabetes. One carbon metabolism which involves the transfer of a methyl group catalyzed by DNA methyltransferase is an important mechanism by which DNA methylation occurs in promoter regions and/or repetitive elements of the genome. Environmental factors may induce epigenetic modification through production of reactive oxygen species, alteration of methyltransferase activity, and/or interference with methyl donors. In this review, we introduce recent studies of epigenetic modification and environmental factors, such as heavy metals, environmental hormones, air pollution, diet and psychosocial stress. We also discuss epigenetic perspectives of early life environmental exposure and late life disease occurrence.

• International Journal of Air-Conditioning and Refrigeration
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• v.9 no.1
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• pp.29-38
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• 2001

In this paper, the defrosting characteristics of a PTC heating sheet is investigated by means of a defrosting heat source for the fin-tube heat exchanger in a refrigerator The defrosting characteristics of the PTC heating sheet are examined and compared with those of a conventional electric heater experimentally. It is found that the characteristics of the water draining rate with the defrosting time show a smoothly oscillating pattern when the PTC heating sheet Is used, and the drained water is completely melted. The defrosting efficiency of the PTC heating sheet is found to be about 75%, which is about 25% higher than that of the electric heater. Also, the reduction of the defrosting time and the increment of the defrosting efficiency may be obtained by improving the arrangement of the heating elements of the healing sheet. It is shown that the defrosting time of the PTC heating sheet increases linearly with the amount of frost, whereas the defrosting efficiency is nearly constant. When applying the PTC heating sheet to the refrigerating system, one should notice the fact that the defrosting performance of the PTC heating sheet may be degraded due to the repetitive operations.

• Journal of the Korea Furniture Society
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• v.24 no.4
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• pp.400-407
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• 2013

21C modern society is a time when enough design to dominate the world, a unique design is required new in all aspects. In addition, there is a religion to the best of Zen art. In our cultural heritage with a ethnicity unique unparalleled in the world, and a new recognition of traditional culture and identity of its own in our country, lattice door flower temple the beauty of Korea in the way you tell, it was modernized by interpreting a new one to utilize as a design element of a wood lattice door flower temple. In this study, to re-appear in openwork having regard to the symbolism of pattern and shape of the lattice door flower temple molding properties were highlighted, change the part of the motif, repeated the curve geometric has been designed to simplify as. As a result, it is possible to present the possibility of a design element that can design a traditional Korean is reinterpreted Modern thereby simplify the repetitive element of the related art, for a new aesthetic changes unique. Design Development of wood with a Buddhist temple flower lattice modern re-interpretation, it suits while a strong indication Korea imagery and expressed a variety of needs and personality to the design elements that are competitive in the world the cage, and design of wood, various studies competitive on to be applied to each field is required.

• Journal of the Korean Society of Costume
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• v.67 no.1
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• pp.110-129
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• 2017

Quality of life today is being enhanced through technology and economic activities, but time for leisure has continued to shrink. In this modern society, a phenomenon is appearing in which people are trying to return to their pure and childlike worlds to relieve themselves from the pressures of stress, anxiety, and feelings of crisis in their repetitive and boring daily lives. Along with the struggle for existence that they feel, this shows how modern people hold on to nostalgic elements experienced in their childhood, and have a tendency to want to express those experiences through consumer activities. As positive opinions stating that such consumer activities not only relieve stress, but also help psychological stability are emerging, consumer trends for the development of fashion products which reflect 'Kidult' emotions are becoming increasingly popular. Not only that, with interior products, the need for the development of differentiated designs which are focused on consumers and which reflect consumer demands is being magnified as they are very important as living designs within the consumption environments of modern people. As such, the goal of this study is to develop textile pattern designs for kidult interior products for adults.

• Korean Institute of Interior Design Journal
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• v.22 no.1
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• pp.38-46
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• 2013

The change in radically changing era requires various interpretation of interior space and application according to it Minimalism shows simple and pure geometric structure and has been influencing to every aspect encompassing space till present. The features of minimalism in space is geometric structure and composition of simple and repetitive line, restrained simple space, pure and structural simplicity. When minimalism construction is discussed, the vocabulary of simplicity has been followed incidentally and has been discussed as concept of simplicity in new viewpoint through historical flow. The purpose of this study is analyzing basic features of John Pawson's ways of working and trying to define the semantics of the new simplicity concept in modern interior space. The new simplicity in John Pawson shows interactive relationship of eastern and western through basic consideration of fundamental elements such as space, light and materials. The relational simple in intermediation of the new simplicity expressed by amalgamation of eastern and western further from disparate interpretation for simplicity expressed in eastern and western is to review back the basic meaning of simplicity in minimalism. And I hope this study can offer various directions for new interior space accepting theory of the new simplicity and I think that new constructional alternative is required as physical space which can be matched with era of social, environmental chaos caused by this theory of the new simplicity.