• Asian Pacific Journal of Cancer Prevention
• /
• v.17 no.3
• /
• pp.1299-1308
• /
• 2016

Our previous studies clearly demonstrated that a combination of WP 631 and Epo B has higher activity against ovarian cancer cells than either of these compounds used separately. In order to fully understand the exact mechanism of action in combination, we assessed effects on the cell cycle of SKOV-3 cells. We evaluated three control points essential for WP 631 and Epo B action to determine which cell cycle-regulating proteins (CDK1/cyclin B complex, EpCAM or HMGB1) mediate activity. The effects of the drug on the cell cycle were measured based on the nuclear DNA content using flow cytometry. Expression of cell cycle-regulating genes was analyzed using real-time PCR. It was discovered that WP 631, at the tested concentration, did not affect the SKOV-3 cell cycle. Epo B caused significant G2/M arrest, whereas the drug combination induced stronger apoptosis and lower mitotic arrest than Epo B alone. This is very important information from the point of view of the fight against cancer, as, while mitotic arrest in Epo B-treated cells could be overcame after DNA damage repair, apoptosis which occurs after mitotic slippage in combination-treated cells is irreversible. It clearly explains the higher activity of the drug combination in comparison to Epo B alone. Epo B acts via the CDK1/cyclin B complex and has the ability to inhibit CDK1, which may be a promising strategy for ovarian cancer treatment in the future. The drug combination diminishes EpCAM and HMGB1 expression to a greater degree than either WP 631 and Epo B alone. Owing to the fact that the high expression of these two proteins is a poor prognostic factor for ovarian cancer, a decrease in their expression, observed in our studies, may result in improved efficacy of cancer therapy. The presented findings show that the combination of WP 631 and Epo B is a better therapeutic option than either of these drugs alone.

• Journal of Industrial Technology
• /
• v.24 no.A
• /
• pp.215-226
• /
• 2004

A dynamic reliability model which can take into account the time history of loading sequences may be applied to the analyses of the hydraulic stability of armor units on rubble-mound breakwaters. All the parameters related to the stability of structures have been considered to be constants in the deterministic model until now. Thus, it is impossible to study the effects of some uncertainties of the related random variables on the stability of structures. In this paper, the dynamic reliability model can be developed by POT(Peak Over Threshold) method in order to take into account the time history of loading sequences and to investigate the temporal behaviors of stability of structure with its loading history. Finally, it is confirmed that the results of dynamic reliability model agree with straight- forwardly those of AFDA(Approximate Full Distribution Approach) of the static reliability model for the same input conditions. In addition, the temporal behaviors of probability of failure can be studied by the dynamic reliability model developed to analyze the hydraulic stability of armor units on rubble-mound breakwaters. Therefore, the present results may be useful for the management of repair and maintenance over the whole life cycle of structure.

• Journal of the Korean Society for Aviation and Aeronautics
• /
• v.23 no.4
• /
• pp.117-124
• /
• 2015

RAM (Reliability, Availability and Maintenance) has been applied to design and analysis tools which affects system's operational sustainability and its life cycle cost as RAM-c(2009 DoD). RAM-c plays also an important role to guarantee the system engineering for mission assurance. Reliability is highly related to the probability of system failure. Availability means mission capability or the condition of ready to mission. Maintenance includes both the repair to recover the system in the event of failure/unexpected breakdown and proactive maintenance to prolong the design service life of the system or machinery. It is the purpose that this paper is to analyze and conclude the objective service life of UAV. The more UAV is operated, the less the level of its reliability becomes. Repairing failures and supplying spare parts on time, system reliability could be improved up until the time over target. Applying statistical Weibull distributions, this paper suggests the analysis of the design service life and economic life of UAV based on RAM-c with operational data.

• Journal of the Korean Society of Manufacturing Process Engineers
• /
• v.20 no.10
• /
• pp.102-111
• /
• 2021

Maintainability is a major design parameter that includes availability as well as reliability in a RAM (reliability, availability, maintainability) analysis, and is an index that must be considered when developing a system. There is a lack of awareness of the importance of predicting and analyzing maintainability; therefore, it is dependent on past-experience data. To improve the utilization rate, maintainability must be managed as a key indicator to meet the user's requirements for failure maintenance time and to reduce life-cycle costs. To improve the maintainability-prediction accuracy in the detailed design stage, we present a maintainability-prediction method that applies Method B of the Military Standardization Handbook (MIL-HDBK-472) Procedure V, as well as a Korean maintainability-prediction software package that reflects the system complexity.

• Proceedings of the Korean Society for Quality Management Conference
• /
• 1998.11a
• /
• pp.544-555
• /
• 1998

Although the bimodal mixed weibull distribution is used to developing burn-in model widely, the failure times for a component or a system is often truncated at some time, T, due to the obsolescence in the electronics industry. In this paper, we will determine minimum total cost and burn-in time by using the bimodal mixed weibull distribution and the truncated bimodal mixed weibull distribution under the free warranty policy. The results of this study are summarized as follows. First, when products or system is not repairable, the width of the change of burn-in time can be larger by ${\beta}_1,\;{\beta}_2$ Second, if burn-in time become longer, it will be impossible to consider the bum-in in a long time, and in this case, the burn-in time should be shorten by the acceleration burn-in. Third, in case that opportunity loss cost or repair cost is exceed the warranty cost, or the total cost of considering burn-in is larger than that of not considering burn-in, it is not existed burn-in time which makes total cost to minimize. Forth, the shorter life-cycle of product, the more burn-in times will be decreased and the cost in considering burn-in will be increased

• Journal of Korean Society of Disaster and Security
• /
• v.5 no.1
• /
• pp.43-47
• /
• 2012

The asset management of infra facilities is a total framework for finally supporting a safe and comfortable service, which includes functions of supporting evaluation of condition and performance of infrastructures, making the decision method of repair or rehabilitation of deteriorated facilities, and lengthening the life cycle of structure through the decision of adequate cost and time of repair or reinforcement. In the range of the asset management, organization, human, the target, and information & data of company are included. Therefore, in this paper, appling the method of asset management analysis to the infra structures, the process of the risk assesment using BRE (Business Risk Exposure) and the basis of consisting ORDM (Optimized Renewal Decision-Making) are expressed.

• Journal of Radiation Protection and Research
• /
• v.37 no.2
• /
• pp.56-62
• /
• 2012

To determine the biological effects of low-dose-rate radiation ($^{137}Cs$, 2.95 mGy/h) on EL4 lymphoma cells during 24 h, we investigated the expression of genes related to apoptosis, cell cycle arrest, DNA repair, iron transport, and ribonucleotide reductase. EL4 cells were continuously exposed to low-dose-rate radiation (total dose: 70.8 mGy) for 24 h. We analyzed cell proliferation and apoptosis by trypan blue exclusion and flow cytometry, gene expression by real-time PCR, and protein levels with the apoptosis ELISA kit. Apoptosis increased in the Low-dose-rate irradiated cells, but cell number did not differ between non- (Non-IR) and Low-dose-rate irradiated (LDR-IR) cells. In concordance with apoptotic rate, the transcriptional activity of ATM, p53, p21, and Parp was upregulated in the LDR-IR cells. Similarly, Phospho-p53 (Ser15), cleaved caspase 3 (Asp175), and cleaved Parp (Asp214) expression was upregulated in the LDR-IR cells. No difference was observed in the mRNA expression of DNA repair-related genes (Msh2, Msh3, Wrn, Lig4, Neil3, ERCC8, and ERCC6) between Non-IR and LDR-IR cells. Interestingly, the mRNA of Trfc was upregulated in the LDR-IR cells. Therefore, we suggest that short-term Low-dose-rate radiation activates apoptosis in EL4 lymphoma cells.

• Journal of the Korean Association of Oral and Maxillofacial Surgeons
• /
• v.45 no.5
• /
• pp.285-293
• /
• 2019

Objectives: This study examined the effects of plasma-rich growth factors (PRGF) on accelerating bone regeneration/repair in fresh extraction sockets, and determined the quality and quantity of bone by assessing the bone density using cone-beam computed tomography (CBCT). Materials and Methods: Twenty patients, who had undergone bilateral extractions, were included in this study. In one extraction socket, PRGF was used and covered with an autologous fibrin plug. Nothing was used in the opposite side extraction socket. Thirteen weeks post extraction, the level of bone regeneration was evaluated on both sides with CBCT. Results: At the end of the study, the mean bone density according to the Hounsfield units (HU) in the control group and PRGF group was 500.05 HU (type III bone type) and 647.95 HU (type II bone type), respectively. Conclusion: This study recommends the use of PRGF in post extraction sites to accelerate the rate of bone regeneration and improve the quality of regenerated bone. The technique to process PRGF was simple compared to previously mentioned techniques used for platelet-rich plasma (PRP) preparation. PRP preparation requires a two-cycle centrifugation procedure, leading to a longer processing time.

• Journal of the Korea Academia-Industrial cooperation Society
• /
• v.19 no.12
• /
• pp.717-724
• /
• 2018

To prepare for the explosive increase in maintenance costs of bridges according to the aging of infrastructure, future maintenance costs of bridges should be predicted. For this purpose, the management status of bridges was investigated and modeled as the upper limit of the performance level and the target management level according to the life cycle. This paper proposes methodologies and procedures for estimating the bridge maintenance costs using two models and existing cost and performance prediction models that consist of unit repair cost model according to the safety score, performance degradation model of bridges, unit reconstruction cost, and average reconstruction time. To verify the applicability, future maintenance costs can be forecasted for specific management agency considering the number of bridges, degree of aging, and current management status. As a result, it is possible to obtain the maintenance cost and safety level of an individual bridge level for each year. In addition, by summing them up to the agency level, the average safety score, ratio of the safety level, inspection costs, repair costs, and reconstruction costs can be obtained. In a further study, the changes in maintenance costs can be analyzed according to the changes in the target management levels using the developed method. The optimal management level can be suggested by reviewing the results.

• Journal of the Korea institute for structural maintenance and inspection
• /
• v.11 no.6
• /
• pp.131-142
• /
• 2007

The road user cost in indirect costs as well as direct costs such as the inspection/ diagnosis cost and the repair/reinforcement cost should be considered as one of the important items in the life-cycle cost-effective design and maintenance of the bridges. To estimate the road user cost, this paper formulates the road user cost as a sum of the user delay cost and the vehicle operating cost considering the detour effect. A numerical traffic simulation and a regression analysis are performed to develop a regression model due to a time delay. The proposed regression model is applied to the generation of the maintenance strategy based on the life-cycle cost and performance, and its effectiveness and applicability is investigated. The road user cost has a great influence on establishing the maintenance strategy, and the proposed regression model could be successfully utilized to estimate the road user cost of a bridge.