Journal of the Korean Association of Oral and Maxillofacial Surgeons

The Korean Association of Oral and Maxillofacial Surgeons (대한구강악안면외과학회)
권호 표지
DOI QR코드

DOI QR Code

Reduction in post extraction waiting period for dental implant patients using plasma rich in growth factors: an in vivo study using cone-beam computed tomography

  • Arya, Varun (Department of Oral and Maxillofacial Surgery, Faculty of Dental Sciences, SGT University) ;
  • Malhotra, Vijay Laxmy (Department of Dentistry, Shaheed Hasan Khan Mewati (SHKM), Govt. Medical College) ;
  • Rao, JK Dayashankara (Department of Oral and Maxillofacial Surgery, Faculty of Dental Sciences, SGT University) ;
  • Kirti, Shruti (Department of Dentistry, Shaheed Hasan Khan Mewati (SHKM), Govt. Medical College) ;
  • Malhotra, Siddharth (Private Clinic) ;
  • Sharma, Radhey Shyam (Department of Dentistry, Shaheed Hasan Khan Mewati (SHKM), Govt. Medical College)
  • Received : 2018.08.25
  • Accepted : 2018.12.20
  • Published : 2019.10.31
Download PDF
⟨ Previous Next ⟩

Abstract

Objectives: This study examined the effects of plasma-rich growth factors (PRGF) on accelerating bone regeneration/repair in fresh extraction sockets, and determined the quality and quantity of bone by assessing the bone density using cone-beam computed tomography (CBCT). Materials and Methods: Twenty patients, who had undergone bilateral extractions, were included in this study. In one extraction socket, PRGF was used and covered with an autologous fibrin plug. Nothing was used in the opposite side extraction socket. Thirteen weeks post extraction, the level of bone regeneration was evaluated on both sides with CBCT. Results: At the end of the study, the mean bone density according to the Hounsfield units (HU) in the control group and PRGF group was 500.05 HU (type III bone type) and 647.95 HU (type II bone type), respectively. Conclusion: This study recommends the use of PRGF in post extraction sites to accelerate the rate of bone regeneration and improve the quality of regenerated bone. The technique to process PRGF was simple compared to previously mentioned techniques used for platelet-rich plasma (PRP) preparation. PRP preparation requires a two-cycle centrifugation procedure, leading to a longer processing time.

Keywords

References

  1. Thor A, Wannfors K, Sennerby L, Rasmusson L. Reconstruction of the severely resorbed maxilla with autogenous bone, platelet-rich plasma, and implants: 1-year results of a controlled prospective 5-year study. Clin Implant Dent Relat Res 2005;7:209-20. https://doi.org/10.1111/j.1708-8208.2005.tb00066.x
  2. Del Fabbro M, Boggian C, Taschieri S. Immediate implant placement into fresh extraction sites with chronic periapical pathologic features combined with plasma rich in growth factors: preliminary results of single-cohort study. J Oral Maxillofac Surg 2009;67:2476-84. https://doi.org/10.1016/j.joms.2009.04.063
  3. Lekholm U, Zarb GA. Patient selection and preparation. In: Branemark PI, Zarb GA, Albrektsson T, eds. Tissue-integrated prostheses: osseointegration in clinical dentistry. Chicago: Quintessence; 1985:199-209.
  4. Anitua E, Orive G, Andia I. Use of PRGF to accelerate bone and soft tissue regeneration in postextraction sites: evaluation of regenerated bone density. Implant Dialog 2003;36:3-14.
  5. Irinakis T. Rationale for socket preservation after extraction of a single-rooted tooth when planning for future implant placement. J Can Dent Assoc 2006;72:917-22.
  6. Lekovic V, Camargo PM, Klokkevold PR, Weinlaender M, Kenney EB, Dimitrijevic B, et al. Preservation of alveolar bone in extraction sockets using bioabsorbable membranes. J Periodontol 1998;69:1044-9. https://doi.org/10.1902/jop.1998.69.9.1044
  7. Anitua E. The use of plasma-rich growth factors (PRGF) in oral surgery. Pract Proced Aesthet Dent 2001;13:487-93; quiz 487-93.
  8. Anitua E, Alkhraisat MH, Pinas L, Orive G. Efficacy of biologically guided implant site preparation to obtain adequate primary implant stability. Ann Anat 2015;199:9-15. https://doi.org/10.1016/j.aanat.2014.02.005
  9. Deuel TF, Huang JS, Proffitt RT, Baenziger JU, Chang D, Kennedy BB. Human platelet-derived growth factor. Purification and resolution into two active protein fractions. J Biol Chem 1981;256:8896-9. https://doi.org/10.1016/S0021-9258(19)52482-6
  10. Marx RE. Platelet-rich plasma: evidence to support its use. J Oral Maxillofac Surg 2004;62:489-96. https://doi.org/10.1016/j.joms.2003.12.003
  11. Marx RE, Carlson ER, Eichstaedt RM, Schimmele SR, Strauss JE, Georgeff KR. Platelet-rich plasma: growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 1998;85:638-46. https://doi.org/10.1016/S1079-2104(98)90029-4
  12. Sammartino G, Tia M, Marenzi G, di Lauro AE, D'Agostino E, Claudio PP. Use of autologous platelet-rich plasma (PRP) in periodontal defect treatment after extraction of impacted mandibular third molars. J Oral Maxillofac Surg 2005;63:766-70. https://doi.org/10.1016/j.joms.2005.02.010
  13. Celio-Mariano R, de Melo WM, Carneiro-Avelino C. Comparative radiographic evaluation of alveolar bone healing associated with autologous platelet-rich plasma after impacted mandibular third molar surgery. J Oral Maxillofac Surg 2012;70:19-24. https://doi.org/10.1016/j.joms.2011.03.028
  14. Alissa R, Esposito M, Horner K, Oliver R. The influence of platelet-rich plasma on the healing of extraction sockets: an explorative randomised clinical trial. Eur J Oral Implantol 2010;3:121-34.
  15. Antonello Gde M, Torres do Couto R, Giongo CC, Correa MB, Chagas Junior OL, Lemes CH. Evaluation of the effects of the use of platelet-rich plasma (PRP) on alveolar bone repair following extraction of impacted third molars: prospective study. J Craniomaxillofac Surg 2013;41:e70-5. https://doi.org/10.1016/j.jcms.2012.11.003
  16. Kawase T. Platelet-rich plasma and its derivatives as promising bioactive materials for regenerative medicine: basic principles and concepts underlying recent advances. Odontology 2015;103:126-35. https://doi.org/10.1007/s10266-015-0209-2
  17. Muntean W, Zenz W, Finding K, Zobel G, Beitzke A. Inhibitor to factor V after exposure to fibrin sealant during cardiac surgery in a two-year-old child. Acta Paediatr 1994;83:84-7.
  18. Weibrich G, Kleis WK, Hitzler WE, Hafner G. Comparison of the platelet concentrate collection system with the plasma-richin-growth-factors kit to produce platelet-rich plasma: a technical report. Int J Oral Maxillofac Implants 2005;20:118-23.
  19. Gurbuzer B, Pikdoken L, Tunali M, Urhan M, Kucukodaci Z, Ercan F. Scintigraphic evaluation of osteoblastic activity in extraction sockets treated with platelet-rich fibrin. J Oral Maxillofac Surg 2010;68:980-9. https://doi.org/10.1016/j.joms.2009.09.092
  20. Nishiyama K, Okudera T, Watanabe T, Isobe K, Suzuki M, Masuki H, et al. Basic characteristics of plasma rich in growth factors (PRGF): blood cell components and biological effects. Clin Exp Dent Res 2016;2:96-103. https://doi.org/10.1002/cre2.26
  21. Choukroun J, Diss A, Simonpieri A, Girard MO, Schoeffler C, Dohan SL, et al. Platelet-rich fibrin (PRF): a second-generation platelet concentrate. Part IV: clinical effects on tissue healing. Oral Surg Oral Med Oral Pathol Oral Radiol Endod 2006;101:e56-60. https://doi.org/10.1016/j.tripleo.2005.07.011
  22. Al-Hamed FS, Tawfik MA, Abdelfadil E, Al-Saleh MAQ. Efficacy of platelet-rich fibrin after mandibular third molar extraction: a systematic review and meta-analysis. J Oral Maxillofac Surg 2017;75:1124-35. https://doi.org/10.1016/j.joms.2017.01.022
  23. Choukroun J. Advanced PRF, & i-PRF: platelet concentrates or blood concentrates? J Periodontal Med Clin Pract 2014;1:3.
  24. Masuki H, Okudera T, Watanebe T, Suzuki M, Nishiyama K, Okudera H, et al. Growth factor and pro-inflammatory cytokine contents in platelet-rich plasma (PRP), plasma rich in growth factors (PRGF), advanced platelet-rich fibrin (A-PRF), and concentrated growth factors (CGF). Int J Implant Dent 2016;2:19. https://doi.org/10.1186/s40729-016-0052-4
  25. Dohan Ehrenfest DM, Rasmusson L, Albrektsson T. Classification of platelet concentrates: from pure platelet-rich plasma (P-PRP) to leucocyte- and platelet-rich fibrin (L-PRF). Trends Biotechnol 2009;27:158-67. https://doi.org/10.1016/j.tibtech.2008.11.009
  26. Magalon J, Chateau AL, Bertrand B, Louis ML, Silvestre A, Giraudo L, et al. DEPA classification: a proposal for standardising PRP use and a retrospective application of available devices. BMJ Open Sport Exerc Med 2016;2:e000060. https://doi.org/10.1136/bmjsem-2015-000060
  27. Anitua E. Plasma rich in growth factors: preliminary results of use in the preparation of future sites for implants. Int J Oral Maxillofac Implants 1999;14:529-35.
  28. Fuerst G, Gruber R, Tangl S, Sanroman F, Watzek G. Enhanced bone-to-implant contact by platelet-released growth factors in mandibular cortical bone: a histomorphometric study in minipigs. Int J Oral Maxillofac Implants 2003;18:685-90.
  29. Anitua E, Murias-Freijo A, Alkhraisat MH, Orive G. Clinical, radiographical, and histological outcomes of plasma rich in growth factors in extraction socket: a randomized controlled clinical trial. Clin Oral Investig 2015;19:589-600. https://doi.org/10.1007/s00784-014-1278-2
  30. Wang HL, Pappert TD, Castelli WA, Chiego DJ Jr, Shyr Y, Smith BA. The effect of platelet-derived growth factor on the cellular response of the periodontium: an autoradiographic study on dogs. J Periodontol 1994;65:429-36. https://doi.org/10.1902/jop.1994.65.5.429
  31. Gruber R, Varga F, Fischer MB, Watzek G. Platelets stimulate proliferation of bone cells: involvement of platelet-derived growth factor, microparticles and membranes. Clin Oral Implants Res 2002;13:529-35. https://doi.org/10.1034/j.1600-0501.2002.130513.x
  32. Weibrich G, Gnoth SH, Otto M, Reichert TE, Wagner W. [Growth stimulation of human osteoblast-like cells by thrombocyte concentrates in vitro]. Mund Kiefer Gesichtschir 2002;6:168-74. German. https://doi.org/10.1007/s10006-002-0367-6
  33. Anitua E, Prado R, Troya M, Zalduendo M, de la Fuente M, Pino A, et al. Implementation of a more physiological plasma rich in growth factor (PRGF) protocol: Anticoagulant removal and reduction in activator concentration. Platelets 2016;27:459-66. https://doi.org/10.3109/09537104.2016.1143921
  34. Mozzati M, Gallesio G, di Romana S, Bergamasco L, Pol R. Efficacy of plasma-rich growth factor in the healing of postextraction sockets in patients affected by insulin-dependent diabetes mellitus. J Oral Maxillofac Surg 2014;72:456-62. https://doi.org/10.1016/j.joms.2013.10.010
  35. Haraji A, Lassemi E, Motamedi MH, Alavi M, Adibnejad S. Effect of plasma rich in growth factors on alveolar osteitis. Natl J Maxillofac Surg 2012;3:38-41. https://doi.org/10.4103/0975-5950.102150
  36. Anitua E, Prado R, Orive G. Bilateral sinus elevation evaluating plasma rich in growth factors technology: a report of five cases. Clin Implant Dent Relat Res 2012;14:51-60. https://doi.org/10.1111/j.1708-8208.2009.00233.x

Cited by

  1. Platelet rich plasma in oral and maxillofacial surgery from the perspective of composition vol.32, pp.2, 2019, https://doi.org/10.1080/09537104.2020.1856361