• International Journal of CAD/CAM
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• 제3권1_2호
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• pp.13-17
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• 2003

Hybrid method of representing geometric entities as combination of boundary (B-rep) and functional (F-rep) representations is presented which can be used as a basis of solid modeling kernel. It contains whole functionality of classic B-rep kernel, and also supports enveloping (sweep of solid body). Principles and keysolutions are considered. Example of a real task that comes from gearing is provided.

• Journal of Microbiology and Biotechnology
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• 제17권11호
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• pp.1841-1847
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• 2007

We previously identified the origin of replication of p703/5, a small cryptic plasmid from the KBL703 strain of Enterococcus faecalis. The origin of replication contains putative regulatory cis-elements required for replication and a replication initiator (RepA) gene. The replicon of p703/5 is similar in its structural organization to theta-type plasmids, and RepA is homologous to a family of Rep proteins identified in several plasmids from Gram-positive bacteria. Here, we report molecular interactions between RepA and the replication origin of p703/5. DNase I footprinting using recombinant RepA together with electrophoretic mobility shift assays confirmed the binding of RepA to the replication origin of p703/5 via iterons and an inverted repeat. We also demonstrated the formation of RepA dimers and the different binding of RepA to the iteron and the inverted repeat using gel filtration chromatographic analysis, a chemical crosslinking assay, and electrophoretic mobility shift assays in the presence of guanidine hydrochloride. Our results suggest that RepA plays a regulatory role in the replication of the enterococcal plasmid p703/5 via mechanisms similar to those of typical iteroncarrying theta-type plasmids.

• 한국정밀공학회지
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• 제13권3호
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• pp.150-157
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• 1996

In this paper, to develop a smart CAD/CAM system for systematically performing from the 3-D solid shape design of products to the CNC cutting operation of products by a machining center, a B-Rep solid modeler is realized based on the half edge data structure. Because the B-Rep solid modeler has the various capabilities related to the solid definition functions such as the creation operation of primitives and the translational and rotational sweep operation, the solid manipulation functions such as the split operation and the Boolean set operation, and the solid inversion function for effectively using the data structure, the 3-D solid shape of products can be easily designed and constructed. Also, besides the automatic generation of CNC code, the B-Rep solid modeler can be used as a powerful tool for realizing the automatic generation of finite elements, the interfer- ence check between solids, the structural design of machine tools and robots and so on.

• 한국식품과학회지
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• 제37권4호
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• pp.611-617
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• 2005

본 연구에서는 E. coli. Salmonella, Shigella, Vibrio 등 4속의 주요 식중독유발 그람 음성 세균들을 대상으로 반복성 염기서열인 REP DNA sequence를 응용한 REP-PCR을 실시하였다. 이전의 보고에서 이들 4속의 식중독 유발세균 중 각각 혹은 일부를 대상으로 반복성 염기서열을 이용한 PCR을 적용한 사례는 있지만 그때 적용한 primer, PCR 반응조건 및 전기영동조건 등이 다양하였다. 그러므로 본 연구에서는 이와같은 4속의 세균들에 대하여 최적화된 동일한 primer와 PCR 반응조건 및 전기영동조건을 표준조건으로서 적용하였다. 그 결과로서 모든 4속의 식중독 세균 균주마다 REP-PCR 후 생성되는 fingerprinting pattern에서 속마다 1-3개의 공통적이며 독특한 band가 생성되는 것이 확인되어 이러한 pattern을 이용한 속 수준의 분리 동정과 그와 같은 주요 band들 이외의 부수적인 band들을 고려하여 종 수준까지의 분리도 가능함을 확인하였다. 따라서 본 연구를 통하여 반복적 DNA 염기서열을 이용한 REP-PCR이 주요 식중독 세균의 분리 동정 방법으로 사용될 수 있음을 확인하였다. 또한 본 연구를 통하여 얻은 결과는 더 많은 속(genus)의 식중독세균을 대상으로 한 새로운 분리 동정 방법을 확립하기 위하여 사용될 수 있을 것이다.

• 생명과학회지
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• 제34권2호
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• pp.86-93
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• 2024

플라스미드의 복제는 엄격하게 조절되어야 하기 때문에 일반적으로 rolling circle 복제를 수행하는 플라스미드들은 복제 개시인자인 RepB는 전사 및 번역 수준에서 엄격하게 조절되어 일정한 copy number를 유지한다. 플라스미드 pJB01에는 단일 오페론으로 구성된 세 개의 orfs (copA, repB, repC 또는 repABC)가 포함되어 있다. 아미노산 서열 분석에서 pJB01 CopA는 다른 플라스미드의 복제 수 조절 단백질로서 Cops와 상동성을 보였다. pMV158의 CopG와 비교할 때, CopA는 플라스미드의 일반화된 억제자의 모티브로 알려진 RHH (ribbon-helix-helix)를 형성하는 것으로 추정된다. gel mobility shift assay 결과 정제된 융합 단백질이 repABC 오페론의 operator 영역에 결합하는 것으로 나타났다. 전사 수준에 대한 CopA의 기능적 역할을 조사하기 위해 CopA R16M, K26R 및 E50V와 같은 세 개의 포인트 돌연변이가 CopA의 코딩 프레임에서 구성되었다. CopA R16M, K26R 및 E50V 돌연변이의 repABC mRNA 수준은 CopA wt보다 각각 1.84, 1.78 및 2.86배 증가했다. 또한 세 개의 CopA 유전자의 돌연변이로 인한 복제 수도 CopA wt보다 각각 1.86, 1.68 및 2.89배 증가했다. 이러한 결과는 CopA가 전사 억제자이며 복제 개시자로서 repABC mRNA 및 RepB 단백질 수를 감소시킴으로써 pJB01의 복제 수를 감소시키는 것으로 제의된다.

• Journal of the Optical Society of Korea
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• 제7권3호
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• pp.131-134
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• 2003

We demonstrated an optical frequency synthesizer based on a femtosecond (fs) mode-locked Ti:sapphire (Ti:s) laser by simultaneously stabilizing the carrier-offset frequency, $f_{ceo}$, and repetition rate, $f_{ rep}$, referenced to the Cs atomic frequency standard. By using two wide-band digital phase-detectors we realized a phase-coherent link between $f_{rep} and f_{ceo} with the relation f_{ceo} = f_{AOM} 5/6f_{rep} ≡ 0, where f_{AOM} = 5/6f_{rep}$ is the phase-locked driving frequency of an acousto-optic modulator (AOM) in a self-referencing interferometer and $f_{rep}$ = 100 MHz. As a result, we could stabilize all components of the fs laser comb at once with an equal frequency separation $f_{rep}$ = 100 MHz with $f_{ceo}$ = 0. In our optical frequency synthesizer, the frequency of the nth component ($f_{n}$) is given exactly by the simple relation $f_n = nf_{rep}$, enabling us to use the fs laser comb as a frequency ruler in the optical frequency metrology.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 1994년도 추계학술대회 논문집
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• pp.689-694
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• 1994

In this paper, to develop a smart CAD/CAM system for systematically performing from the 3-D solid shape design of products to the CNC cutting operation of products by a machining center, a B-Rep solid modeler is realized based on the half edge data structure. Because the B-Rep solid modeler has the various capabilities related to the solid definition functions such as the creation operation of primitives and the translational and rotational sweep operation, the solid manipulation functions such as the split operation and the Boolean set operation, and the solid inversion function for effectively using the data structure, the 3-D solid shape of products can be easily designed and constructed. Also, besides the automatic generation of CNC code, the B-Rep solid modeler can be used as a powerful tool for realizing the automatic generation of finite elements, the interference check between solids, the structural design of machine tools and robots and so on.

• IMMUNE NETWORK
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• 제2권4호
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• pp.195-201
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• 2002

Background: IgE is closely related to the development of allergies. However, the poor relationship between the specific IgE level and the severity of allergic diseases suggests the possibility of functionally different IgE isoforms. With this in mind, rat basophilic leukemia (RBL)-2H3 activation was analyzed with each type of rat IgE for two parameters, exocytosis and IL-4 mRNA production. RBL-2H3 has been well documented in the rat mucosal mast cell line. Methods: RBL-2H3 cells sensitized with each kind of rat IgE was activated by cross-linking FcRI with B5 (monoclonal anti-rat IgE mouse IgG antibodies). The RBL-2H3 exocytosis was measured by analyzing the ${\beta}$-hexosaminidase level, and the level of IL-4 mRNA synthesis was analyzed using semiquantitative RT-PCR. Rat IgE, which was produced by a parasite infection (REP), was prepared using either Paragonimus westermani metacercariae (REP-PW) or Anisakis simplex third stage larvae (REP-AS). A rat IgE prototype of IR162 was prepared by a peritoneal injection of immunocytoma. Results: The level of exocytosis showed a linear relationship with the rat IgE concentration when REP-PW or REP-AS was applied. However, it exhibited a biphasic response with IR162. In addition, the time course of heating at $56^{\circ}C$ illustrated the similarity between REP-PW and REP-AS, which differed from that of IR162. In contrast, the level of IL-4 mRNA synthesis in the RBL-2H3 cells with IR162 was comparable to that of either REP-PW or REP-AS. Conclusion: These results suggest that functionally different rat IgE isoforms exists in RBL-2H3 exocytosis.

• 한국지역사회생활과학회지
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• 제27권3호
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• pp.509-520
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• 2016

This study was carried out to investigate the effects of adding rosemary extract powder (REP) to pork teokgalbi on its quality and storage characteristics. Five types of teokgalbi were tested, viz. with no REP added (normal, N), with 0.05% ascorbic acid added (control, C) and with 0.05% (R1), 0.1% (R2) and 0.2% (R3) of REP added. The proximate composition, calorific value, water holding capacity and cooking loss were not significantly different among the different types of pork teokgalbi. The L and a values of the teokgalbi containing REP were significantly lower than those of the N and C groups. As regards the textural characteristics, the addition of 0.2% REP increased the springiness value. In the sensory evaluation, the teokgalbi with the addition of 0.1% REP had higher scores in flavor and total acceptability. The 2-thiobarbituric acid (TBA) value, total microbial count and volatile basic nitrogen content of the teokgalbi containing 0.2% REP were lower than those of the N group. In conclusion, R3 was significantly more effective in delaying lipid peroxidation than the other treatment groups. Therefore, rosemary extract could be used as a natural antioxidant in meat products.

• Journal of Microbiology and Biotechnology
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• 제27권10호
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• pp.1778-1782
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• 2017

To identify and discriminate the bacterial species at the subspecific level, rep-PCR is a reliable genomic fingerprinting tool. Fourteen strains of bacteria were isolated from different food sources, identified as Leuconostoc citreum using 16S rRNA gene sequencing, and amplified using rep-primers (REP, ERIC, and $(GTG)_5$). Fingerprinting patterns generated bands in the range of 300-6,000 bp with REP, 150-6,000 bp with ERIC, and 200-1,700 bp with $(GTG)_5$ primers. In UPGMA dendrogram analysis, 14 strains were clustered into three clades (I, II, and III) with all the primers, thus differentiating them at the molecular level. The present study revealed the differentiation of L. citreum strains using rep-PCR.