• Title/Summary/Keyword: Relative Disparity

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Intraspecies Volatile Interactions Affect Growth Rates and Exometabolomes in Aspergillus oryzae KCCM 60345

  • Singh, Digar;Lee, Choong Hwan
    • Journal of Microbiology and Biotechnology
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    • v.28 no.2
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    • pp.199-209
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    • 2018
  • Volatile organic compounds (VOCs) are increasingly been recognized as the chemical mediators of mold interactions, shaping their community dynamics, growth, and metabolism. Herein, we selectively examined the time-correlated (0 D-11 D, where D = incubation days) effects of intraspecies VOC-mediated interactions (VMI) on Aspergillus oryzae KCCM 60345 (S1), following co-cultivation with partner strain A. oryzae KACC 44967 (S2), in a specially designed twin plate assembly. The comparative evaluation of $S1_{VMI}$ (S1 subjected to VMI with S2) and its control ($S1_{Con}$) showed a notable disparity in their radial growth ($S1_{VMI}$ < $S1_{Con}$) at 5 D, protease activity ($S1_{VMI}$ > $S1_{Con}$) at 3-5 D, amylase activity ($S1_{VMI}$ < $S1_{Con}$) at 3-5 D, and antioxidant levels ($S1_{VMI}$ > $S1_{Con}$) at 3 D. Furthermore, we observed a distinct clustering pattern for gas chromatography-time of flight-mass spectrometry datasets from 5 D extracts of $S1_{VMI}$ and $S1_{Con}$ in principle component analysis (PC1: 30.85%; PC2: 10.31%) and partial least squares discriminant analysis (PLS-DA) (PLS1: 30.77; PLS2: 10.15%). Overall, 43 significantly discriminant metabolites were determined for engendering the metabolic variance based on the PLS-DA model (VIP > 0.7, p < 0.05). In general, a marked disparity in the relative abundance of amino acids ($S1_{VMI}$ > $S1_{Con}$) at 5 D, organic acids ($S1_{VMI}$ > $S1_{Con}$) at 5 D, and kojic acid ($S1_{VMI}$ < $S1_{Con}$) at 5-7 D were observed. Examining the headspace VOCs shared between S1 and S2 in the twin plate for 5 D incubated samples, we observed the relatively higher abundance of C-8 VOCs (1-octen-3-ol, (5Z)-octa-1,5-dien-3-ol, 3-octanone, 1-octen-3-ol acetate) having known semiochemical functions. The present study potentially illuminates the effects of VMI on commercially important A. oryzae's growth and biochemical phenotypes with subtle details of altered metabolomes.

The effect of brightness contrast on resolving the correspondence problem (상의 대응 문제 해결에 미치는 밝기 대비의 영향)

  • 감기택;정찬섭
    • Korean Journal of Cognitive Science
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    • v.12 no.4
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    • pp.49-56
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    • 2001
  • When multiple features are presented in the image the computational models for stereopsis select the most activated matches through the excitatory and inhibitory interactions among all possible matches. Using the random-dot stereogram with two surfaces. we investigate whether human binocular mechanism selects the most activated matches. The dots consisting a surface lying in a fixation plane were selected randomly while each of the dots consisting the other surface was paired with each of the original dots in the following manner. After finding the position of each dots in the original random pattern we placed an additional dot to the left and to the right of the original position in each of the left and right image of a stereogram respectively. The luminance of additional dots was varied while that of the original random dots was fixed so that the hypothetical matches presumably could be activated differently. Across the luminance condition the depth of each surface was measured to examine whether matches to be selected were changed depending on the activation level of possible matches. When the luminance of two patterns was within 30% of one another observers perceived an opaque surface. Beyond this value two transparent surfaces were seen with the magnitude of perceived depth varying with relative luminance of two patterns. When original pattern was brighter one additional surface was perceived at the depth corresponding to the disparity of original pattern. When original dot was dimmer. however the depth of an additional surface corresponded to the disparity of newly introduced pattern. These results suggest that there are dynamic interactions within the matching process whereby highly activated matches inhibit weaker one.

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Autonomous Mobile Robot System Using Adaptive Spatial Coordinates Detection Scheme based on Stereo Camera (스테레오 카메라 기반의 적응적인 공간좌표 검출 기법을 이용한 자율 이동로봇 시스템)

  • Ko Jung-Hwan;Kim Sung-Il;Kim Eun-Soo
    • The Journal of Korean Institute of Communications and Information Sciences
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    • v.31 no.1C
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    • pp.26-35
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    • 2006
  • In this paper, an automatic mobile robot system for a intelligent path planning using the detection scheme of the spatial coordinates based on stereo camera is proposed. In the proposed system, face area of a moving person is detected from a left image among the stereo image pairs by using the YCbCr color model and its center coordinates are computed by using the centroid method and then using these data, the stereo camera embedded on the mobile robot can be controlled for tracking the moving target in real-time. Moreover, using the disparity map obtained from the left and right images captured by the tracking-controlled stereo camera system and the perspective transformation between a 3-D scene and an image plane, depth information can be detected. Finally, based-on the analysis of these calculated coordinates, a mobile robot system is derived as a intelligent path planning and a estimation. From some experiments on robot driving with 240 frames of the stereo images, it is analyzed that error ratio between the calculated and measured values of the distance between the mobile robot and the objects, and relative distance between the other objects is found to be very low value of $2.19\%$ and $1.52\%$ on average, respectably.

Unemployment Disparities and Their Dynamics of the Metropolitan Areas since the Financial Crisis of 1997 (외환위기 이후 대도시지역간 실업의 차이와 그 역동성: 사회적 배제의 구조화에 대한 함의)

  • Lee, Won-Ho
    • Journal of the Economic Geographical Society of Korea
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    • v.11 no.1
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    • pp.94-110
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    • 2008
  • This study examines the unemployment and labor market demand dynamics as well as their implication for social exclusion in the metropolitan areas of Korea since the financial crisis of 1997. The unemployment research containing significant implication for social exclusion is a key area to be explored with the research of skill and income polarization due to structural economic transformation. Skill polarization usually results in the job loss for some people, which most likely leads to the economic deprivation and social exclusion. The unemployment rate and its regional disparity began to fall since 2000, but the disparity reversed to increase after 2005. The labor market dynamics of the metropolitan areas are turned out to be related with the size of the city and the relative shares of both manufacturing and service sectors. In addition, the employment growth is turned out to be related with the changes of both output and productivity. It is also found that the unemployment is affected with the job change and the tertiarization of the economy. However, it is of more significance to recognize that the dynamics and patterns of the labor market in the metropolitan areas are quite spatially differentiated and the differentiation is likely determined by the factors such as industrial structure, employment dynamics and job demand changes.

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A Study on Achievement and Learning Attitude Through Task Learning by Level (수준별 과제학습을 통한 학력신장과 학습태도에 대한고찰)

  • 이종연;이창수
    • Journal of Educational Research in Mathematics
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    • v.9 no.1
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    • pp.279-294
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    • 1999
  • The level-based task learning had an effect on enhancing the math achievement of enrichment and ordinary classes. Besides, the analysis of mathematical attitude change showed that the level-based task learning took effect in the experimental class in every domain, including self-confidence, flexibility, will power, reaction and value, while it made little difference to the comparative class. The findings were as follows in detail. 1. The Outcome of the Achievement Test 1) The Enrichment Class In the first two tests, there were little differences in the enrichment class, But the disparity between the experimental and comparative classes became larger as this study advanced with 4.3 for the third test, 6.4 for the fourth and 6.1 for the fifth. 2) The Ordinary Class In the first to fifth achievement tests, the ordinary class made less difference than the enrichment class did. But there appeared some effect as this study progressed, since the mean grade disparity between the experimental and comparative classes was 2.1 for the first test, 3.5 for the second, 3.9 for the third, 4.4 for the fourth and 6.3 for the fifth. 3) The Supplementary Class The supplementary class showed no big difference in the first two tests. But, like the ordinary class, there was some effect with the lapse of the third 2.9 for the test, 3.2 for the fourth and 4.1 for the fifth. 2. The Change of Mathematical Attitude 1) The Experimental Class The task learning by level had a great deal of effect on the experimental class, as the pre-and post-comparative analyses showed that this class's grades were 5.1 for self-confidence, 10.8 for flexibility, 11.3 for will power, 9.7 for curiosity, 10.9 for reaction and 2.8 for value. 2) The Comparative Class The relative comparison between the comparative class and experimental class revealed that there was a hole effect on the comparative class. 3. The Outcome of Questionnaire Survey 1) They showed a positive reaction, as 40.1% of them answered the level-based task loaming served to raise their achievement, and 48.0% told so-so, and 11.9% replied they weren't helped by it. 2) The results after the experiment were;37.8% of the students say they under- stood practically everything while 12.6% of them say they under stood almost half. 3) The will to learn after the experiment shows dramatic changes between the two classes, The students in the enrichment class showed better will to learn than the students in the ordinary and supplementary classes did.

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Unraveling dynamic metabolomes underlying different maturation stages of berries harvested from Panax ginseng

  • Lee, Mee Youn;Seo, Han Sol;Singh, Digar;Lee, Sang Jun;Lee, Choong Hwan
    • Journal of Ginseng Research
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    • v.44 no.3
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    • pp.413-423
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    • 2020
  • Background: Ginseng berries (GBs) show temporal metabolic variations among different maturation stages, determining their organoleptic and functional properties. Methods: We analyzed metabolic variations concomitant to five different maturation stages of GBs including immature green (IG), mature green (MG), partially red (PR), fully red (FR), and overmature red (OR) using mass spectrometry (MS)-based metabolomic profiling and multivariate analyses. Results: The partial least squares discriminant analysis score plot based on gas chromatography-MS datasets highlighted metabolic disparity between preharvest (IG and MG) and harvest/postharvest (PR, FR, and OR) GB extracts along PLS1 (34.9%) with MG distinctly segregated across PLS2 (18.2%). Forty-three significantly discriminant primary metabolites were identified encompassing five developmental stages (variable importance in projection > 1.0, p < 0.05). Among them, most amino acids, organic acids, 5-C sugars, ethanolamines, purines, and palmitic acid were detected in preharvest GB extracts, whereas 6-C sugars, phenolic acid, and oleamide levels were distinctly higher during later maturation stages. Similarly, the partial least squares discriminant analysis based on liquid chromatography-MS datasets displayed preharvest and harvest/postharvest stages clustered across PLS1 (11.1 %); however, MG and PR were separated from IG, FR, and OR along PLS2 (5.6 %). Overall, 24 secondary metabolites were observed significantly discriminant (variable importance in projection > 1.0, p < 0.05), with most displaying higher relative abundance during preharvest stages excluding ginsenosides Rg1 and Re. Furthermore, we observed strong positive correlations between total flavonoid and phenolic metabolite contents in GB extracts and antioxidant activity. Conclusion: Comprehending the dynamic metabolic variations associated with GB maturation stages rationalize their optimal harvest time per se the related agroeconomic traits.

Ion chromatographic determination of chlorite and chlorate in chlorinated food using a hydroxide eluent

  • Kim, Dasom;Jung, Sungjin;Lee, Gunyoung;Yun, Sang Soon;Lim, Ho Soo;Kim, Hekap
    • Analytical Science and Technology
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    • v.30 no.2
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    • pp.57-67
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    • 2017
  • This study was conducted to develop an analytical technique for determination of chlorite and chlorate concentrations in fresh-cut food and dried fish products by an ion chromatography/conductivity detection method using a hydroxide mobile phase. Deionized water was added to homogenized samples, which were then extracted by ultrasound extraction and centrifuged at high speed (8,500 rpm). Subsequently, a Sep-Pak tC18 cartridge was used to purify the supernatant. Chlorite and chlorate ions were separated using 20 mM KOH solution as the mobile phase and Dionex IonPac AS27 column as the stationary phase. Ethylenediamine was used as sample preservative and dibromoacetate was added to adjust for the disparity in extraction efficiencies between the food samples. The method detection limit) for chlorite and chlorate were estimated to be 0.2 mg/kg and 0.1 mg/kg, respectively, and the coefficient of determination ($r^2$) that denotes the linearity of their calibration curves were correspondingly measured to be 0.9973 and 0.9987. The recovery rate for each ion was 92.1 % and 96.3 %, with relative standard deviations of 7.47 % and 6.18 %, respectively. Although neither chlorite nor chlorate was detected in the food samples, the analytical technique developed in this study may potentially be used in the analysis of disinfected food products.

Preferred Dealy Time and Subjective Preference Judgment for Sound Field with Single Reflection (일차 반사음으로 구성된 음장에서의 최적지연시간과 주관 Preference의 판단)

  • Kang, Seong-Hoon
    • The Journal of the Acoustical Society of Korea
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    • v.7 no.4
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    • pp.5-12
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    • 1988
  • In order to know the preferred delay time of single reflection in relation in relation to the source signal, and to investigate whether or not there is any display in preference judgment of sound field between subjects of different nationalities, tests of subjective preference for musical sound fields with single reflection were preformed. The result showed that the preferred delay times agreed with the effective duration of auto-correlation function of the source signals, when the amplitude of reflection relative to the direct sound is 0dB. No fundamental disparity in series of judgement of sound field was found even for different series of Judgment with different music motifs. The result of preference test using different passages in single music showed that the fluctuation of the effective duration autocorrelation function over all the passages of the music was small. Thus, the preferred delay time can be determined by the coherence of autocorrelation function of the source signals and the amplitued of reflection.

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Implementation of an intelligent vision system for an adaptive path-planning of industrial AGV system (산업용 AGV 시스템의 적응적 경로설정을 위한 지능형 시각 시스템의 구현)

  • Ko, Jung-Hwan
    • 전자공학회논문지 IE
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    • v.46 no.1
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    • pp.23-30
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    • 2009
  • In this paper, the intelligent vision system for an effective and intelligent path-planning of an industrial AGV system based on stereo camera system is proposed. The depth information and disparity map are detected in the inputting images of a parallel stereo camera. The distance between the industrial AGV system and the obstacle detected and the 2D Path coordinates obtained from the location coordinates, and then the relative distance between the obstacle and the other objects obtained from them. The industrial AGV system move automatically by effective and intelligent path-planning using the obtained 2D path coordinates. From some experiments on AGV system driving with the stereo images, it is analyzed that error ratio between the calculated and measured values of the distance between the objects is found to be very low value of 2.5% on average, respectably.

Changes in financial burden of health expenditures by income level (소득 계층별 의료비 부담의 추이와 정책과제)

  • Kim, Tae-Il;Huh, Soon-Im
    • Health Policy and Management
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    • v.18 no.4
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    • pp.23-48
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    • 2008
  • Although the universal health insurance, National Health Insurance (NHI), have improved access to health care and financial burden of health care costs for Koreans, limited coverage of the NHI leads to high out-of-pocket payment for health care. This study examines financial burden of household health expenditures by income level. Data from the Urban Household Expenditure Survey from 1985 through 2005 is analyzed and household expenditure is used as a proxy measure for income. Health expenditures include spending for inpatient care, ambulatory care and pharmaceuticals. If a household spends health expenditure above 40% of household consumption except for foods, that is defined as catastrophic health expenditure. Access to health care for the lowest income group had been improved for two decades relative to other income groups as well as in absolute term. However, both financial burden of health expenditures and the proportion of households that experienced catastrophic health expenditure had been increased in the lowest income group. Study findings have several policy implications. First, in terms of financial burden of health expenditures. the differences among income groups decreased until 2000 but it was worsen in 2005. This suggests that recent policies for extending NHI coverage are not enough to improve the disparity by income level. Second, a differential catastrophic coverage by income level would be an effective strategy that relieves financial burden for low income group. Third, since the catastrophic coverage is applied to only covered services by the NHI, additional strategy for uncovered services should be considered.