• Title/Summary/Keyword: Rehydration rate

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Induced Drought Tolerance by the Insecticide Imidacloprid in Plant (살충제 이미다클로프리드에 의한 식물 가뭄 내성 유도)

  • Han, Song-Hee;Kim, Chul-Hong;Lee, Jang-Hoon;Kim, In-Seon;Kim, Young-Cheol
    • Korean Journal of Environmental Agriculture
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    • v.29 no.2
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    • pp.159-164
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    • 2010
  • Imidacloprid is a systemic insecticide which has been used widely in various crops to control insects. In the present study, we demonstrated that pre-treatment of imidacloprid significantly induced tolerance to drought in plant. Relative water content, chlorophyll levels, and recovery rate upon rehydration after drought stress in tobacco plants pre-treated with imidacloprid were higher levels than the control plants. Induced drought tolerance by imidacloprid treatments in red pepper was also demonstrated by measurement of recovery rate and fresh weight upon drought stress. Taken together, our results suggest that imidacloprid, in addition to exerting direct insecticidal activity, may also protect plants by induced tolerance to drought in plant.

A Trifloxystrobin Fungicide Induces Systemic Tolerance to Abiotic Stresses

  • Han, Song-Hee;Kang, Beom-Ryong;Lee, Jang-Hoon;Lee, Seung-Hwan;Kim, In-Seon;Kim, Chul-Hong;Kim, Young-Cheol
    • The Plant Pathology Journal
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    • v.28 no.1
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    • pp.101-106
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    • 2012
  • Trifloxystrobin is a strobilurin fungicide, which possesses broad spectrum control against fungal plant diseases. We demonstrated that pre-treating red pepper plants with trifloxystrobin resulted in increased plant growth and leaf chlorophyll content compared with those in control plants. Relative water content of the leaves and the survival rate of intact plants indicated that plants acquired systemic tolerance to drought stress following trifloxystrobin pre-treatment. The recovery rate by rehydration in the drought treated plant was better in those pre-treated with trifloxystrobin than that in water treated plants. Induced drought tolerance activity by trifloxystrobin was sustained for 25 days after initial application. The trifloxystrobin treated red pepper plants also had induced systemic tolerance to other abiotic stresses, such as frost, cold, and high temperature stresses. These findings suggest that applying the chemical fungicide trifloxystrobin induced systemic tolerance to certain abiotic stresses in red pepper plants.

Effect of Ethylene Glycol (EG) and 1,2-Propanediol (PROH) on the Survival and the Development of Mouse and Human Embryos after Slow Freezing/Rapid Thawing Protocol (생쥐와 인간배아의 완만동결-급속융해 후 생존률과 배아발생에 미치는 Ethylene Glycol (EG)과 1,2-Propanediol (PROH)의 영향)

  • Kim, Tae-Hyung;Cha, Soo-Kyung;Lee, Dong-Ryul;Han, Jee-Eun;Lee, Woo-Sik;Yoon, Tai-Ki;Cha, Kwang-Yul;Chung, Hyung-Min
    • Clinical and Experimental Reproductive Medicine
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    • v.31 no.1
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    • pp.9-17
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    • 2004
  • Objective: The aim of this study were to compare the effects of EG and PROH on cryopreservation of mouse and human embryos, and to find the optimal protocol for embryo freezing. Methods: Human embryos derived from fertilized eggs showing 3 pronuclei (PN) and mouse embryos were divided into two groups respectively: dehydrated with 1.5 M EG + 0.2 M sucrose or 1.5 M PROH + 0.2 M sucrose using the slow freezing method. Moreover mouse embryos were controlled the exposure time of cryoprotectant during dehydration or rehydration steps. Results: The survival rates of human embryos were 79.2% (84/106) in EG group and 77.9% (88/113) in PROH group. In mouse embryos, the survival and development rates up to blastocyst were 70.6% (245/347), 44.1% (123/279) in EG group and 62.1% (198/319), 45.1% (123/279) in PROH group, respectively. However, in EG group, partially damaged embryos after thawing were decreased compared to PROH group. In combination group, when the exposure time during dehydration and rehydration were reduced, the survival and embryonic developments were increased slightly, but not significant. Conclusion: Cryopreservation of mouse and human embryos at cleavage stage by using EG or PROH exhibited no statistical difference in the survival rate and/or developmental rate to blastocyst. However, the use of EG for cryopreservation of embryos might reduce the exposure time of the cryoprotectant because of a high permeation of EG and result in lessen its toxic effects.

The Change in the Parameters of Silicone Hydrogel Lens and Objective/Subjective Symptoms induced by Repetitive Dryness of Lens (실리콘 하이드로겔 렌즈의 반복적 건조로 인한 렌즈형태 및 자·타각적 증상의 변화)

  • Kim, So Ra;Kang, Byeong Ho;Jung, In Pil;Park, Mijung
    • Journal of Korean Ophthalmic Optics Society
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    • v.17 no.4
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    • pp.381-388
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    • 2012
  • Purpose: In this study, the changes in the shape of silicone hydrogel lenses, dryness of lens and objective/ subjective symptoms that could be induced by repeating dryness of lens and objective/subjective symptoms were investigated. Methods: After drying and rehydrating of silicone hydrogel lenses with different lens material and thickness for 4 times, their overall diameters and base curves were compared. Subjective symptoms, non-invasive tear break-up time (NIBUTs) and blinking rate were evaluated after wearing dehydrated silicone hydrogel lens. Results: Overall diameter and base curve increased in all tested silicone hydrogel lenses by repeating dryness and rehydration. The degree of change in over all diameter and base curve were variable on the material. There were irregular change of lens parameters in thicker lens. When the subjects wore silicone hydrogel lenses after drying and rehydrating, their NIBUTs were decreased and blink rates were increased regardless of lens material or thickness. In addition, repetitive drying of silicone hydrogel lenses affected the lens fitting. Therefore, subjective symptoms such as itching, pain, irritation, foreign body sensation, dryness tended to be increased. Conclusions: The results obtained from the study may suggest to develop durable silicone hydrogel contact lenses against dry environment since the changes in parameters of silicone hydrogel lens and the subjective discomfort were observed after repetitive drying and rehydration.

Development of Effective Cryopreservation Method for Mammalian Embryo (포유류 초기 배아의 효율적인 동결 보존 방법에 관한 연구)

  • Kim, Hyun;Cho, Young Moo;Ko, Yeoung-Gyu;Kim, Sung Woo;Seong, Hwan-Hoo;Yamanouchi, Keitaro
    • Journal of Embryo Transfer
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    • v.29 no.3
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    • pp.241-248
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    • 2014
  • This study was carried out to evaluate the effects of embryonic stage, cryoprotectant, and freezing-thawing method on the rates of survival and development of the cryopreserved mouse early embryo and finally to establish the cryopreservation method of surplus embryos obtained during assisted reproductive technology (ART). Two to eight cell embryos were obtained from oviducts of mated $F_1$ hybrid female mice superovulated by PMSG and hCG. Two-step EG, DMSO and 4-step EG, DMSO were used as cryoprotectant and dehydration and rehydration method of embryos, and slow-cooling or rapid-cooling method was used as frozen program. The survival rates of embryos were measured after thawing and rehydration, and the developmental rates of embryos were compared and observed during culturing embryos for 24, 48, 72, 96 hrs. As for the survival and development rates of embryos according to embryonic stage, the survival rate of 2 cell stage in EG and DMSO was significantly higher than 4~8 cell (65.4% versus 61.2%, 81.1% versus 72.5%) (p<0.01, p<0.01), but the development rates of 4~8 cell embryos in EG and DMSO were significantly higher than 2 cell embryos for whole culture period (p<0.01) and the development rates of 4~8 cell embryos in EG were significantly higher than 2 cell embryos in DMSO (p<0.01). As for the survival and development rates of embryos according to cryoprotectant, the survival rate of 2 cell embryo in DMSO was significantly higher than that in EG(77.0% versus 64.4%) (p<0.01), whereas the development rate of embryos was not differ till 24 hrs. The development rate from morular to hatching blastocyst, however, was sinificantly higher in EG than in DMSO during 48 hr (p<0.01). The survival rate of 4~8 cell embryo was 62.5% in EG and 73.3% in DMSO. The development rates of embryo in EG were significantly higher for whole culture periods (p<0.01, 0.05). In respect to the effect of freezing and thawing program on the survival and development rates of embryos, method of slow cooling and rapid thawing was more effective than that of rapid cooling and rapid thawing. The survival rate of embryo in 2 cell stage was higher than in 4~8 cell stage, and EG appears more effective cryoprotectant than DMSO because EG showed better development rates of embryos in 2 and 4~8 cell stage. Moreover, slow cooling and rapid thawing method was considered as the best cryopreservation program.

Properties of Barley for Extrusion Processing (보리의 Extrusion 가공적성)

  • Lee, Dong-Sun;Rha, Cho-Kyun;Suh, Kee-Bong
    • Applied Biological Chemistry
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    • v.25 no.3
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    • pp.119-125
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    • 1982
  • Using piston type extruder, barley flour was extruded at various processing conditions, The used variables were three shear rates (apparent shear rate 118, 534, $1169sec^{-1}$), four extrusion temperatures(90, 120, 150, $180^{\circ}C$) and three moisture contents (15, 25, 35%). The rheological properties and the extrudate quality were monitored in extrusion. Barley flour showed pseudoplastic behavior having average power law index 0.28 in used shear rate range. When viewed from general appearance, die swell, density, water uptake, rehydration swell and gelatinization degree of extrudate, $25{\sim}35%$ moisture and $120^{\circ}C$ temperature was suitable processing condition for noodle-like product, and 25% moisture and $150^{\circ}C$ temperature was good for snack or flake product. Moisture content of the extrudate can be pretty well estimated from energy balance at higher temperature and higher moisture content.

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Effect of Cooking Methods on the Qualities of Quick Cooking Rice (취반방법이 즉석쌀밥의 품질에 미치는 영향)

  • Kim, Jeong-Sang;Lee, Hyun-Yu;Kim, Yung-Myung;Shin, Dong-Hwa
    • Korean Journal of Food Science and Technology
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    • v.19 no.6
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    • pp.480-485
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    • 1987
  • Quick cooking rice was produced by cooking raw, white long grain rice with three different methods which were atmospheric cooking in electric cooker, autoclaving and precooking followed by autoclaving. The drying rate curve showed that cooking methods affected little the drying pattern of the cooked rice except that precooking treatment before autoclaving retarded dehydration. The quick cooking rice prepared by precooking and autoclaving method among three cooking methods showed the fastest rehydration rate and took 7.5 minutes in reaching equilibrium moisture content by soaking in boiling water. And it had less hard and more cohesive texture than the milled rice cooked by conventional method and the quick cooking rice prepared by electric cooking when reconstituted. The microstructure of quick cooking rice was investigated among cooking methods.

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Effect of Short-term Water Restriction on Body Weight, Egg Production, and Immune Response of Local and Commercial Layers in the Late Phase of Production

  • Ahmed, A.S.;Alamer, M.A.
    • Asian-Australasian Journal of Animal Sciences
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    • v.24 no.6
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    • pp.825-833
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    • 2011
  • Forty-five Hisex commercial layers and forty-five local Saudi breed layers were used to determine the acceptable limit of short-term water restriction in the late phase of production, when the problem of high feed and water consumption is expected. The experiment was performed under hot and arid environmental conditions when the layers were at fifty weeks of age. Layers from each breed were randomly assigned in groups of five into nine floor pens. The average environmental temperature was 37.2-$38.6^{\circ}C$, and the relative humidity was between 20 to 37%. The trial was divided into 3 periods; control (1 week), water restriction (2 weeks) and rehydration (1 week). During the restriction period, layers from each breed were divided into three groups that received 20, 40, and 0% restriction of drinking water relative to their consumed water during the control period. During the study, feed and water consumption, body weight, changes in body weight, egg production, primary antibody response to SRBC, and rectal temperature were evaluated. Water restriction did not result in any clear effect on feed intake in either breed, however, commercial layers tended to consume less feed compared to the local breed. Body weight declined with water restriction during the first week of restriction in the commercial breed regardless of rate of restriction, but it was delayed until the second week in the local breed. Water restriction of 40% decreased egg production in both breeds but with a delay of 1 week in the local breed. Antibody level to SRBC was not affected by water restriction in the commercial line while it was highly affected in the local breed. A water restriction of 20% is considered to be an acceptable limit under the current experimental conditions without a negative effect on egg production in both breeds and considering the immune status of the local breed. Whereas, 40% restriction had a negative effect on egg production, and varied effects in the other traits in both breeds.

Evaluation of Self-Healing Performance Using Hydration Model of Portland Cement and Clinker (포틀랜드시멘트와 클링커의 수화모델을 이용한 자기치유 성능평가)

  • Choi, Sang-Hyeon;Park, Byoung-Sun;Cha, Soo-Won
    • Journal of the Korean Recycled Construction Resources Institute
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    • v.8 no.1
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    • pp.81-87
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    • 2020
  • Crack control is essential to increase the durability of concrete significantly. Healing of crack can be controlled by rehydration of unreacted clinkers at the crack surface. In this paper, by comparing the results of isothermal calorimetry test and regression analysis, the Parrot & Killoh's cement hydration model was verified and clink er hydration model was proposed. The composition and quantification of hydration products were simulated by combining kinematic hydration model and thermodynamic model. Hydration simulation was conducted using the verified and proposed hydration model, and the simulation was performed by the substitution rate of clink er. The type and quantity of the final hydration product and healing product were predicted and, in addition, the optimal cementitious material of self-healing concrete was selected using the proposed hydration model.

STUDIES ON THE SHELLFISH PROCESSING 2. Effect of Dehydration Method, EDTA or BHA Treatment on the Pigment Retention and the Water Absorbing Capacity of the Dehydrated Meat of the Surf Clam Mactra Sulcataria REEVE (패류 가공에 관한 연구 2. 건조 방법 및 EDTA, BHA처리가 개량조개 Mactra Sulcataria REEVE의 색소 잔존율 및 흡수율에 미치는 영향에 대하여)

  • LEE Eung-Ho;HUR Jong-Wha
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.3 no.1
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    • pp.33-37
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    • 1970
  • The effect of the dehydration method and EDTA or BHA treatment upon the pigment degradation and water re-absorption of the dehydrated meat of the surf clam Mactra Sulcataria REEVE was studied. Sun drying Produced a realer decrease in pigment content as compared to that produced by hot-air dehydration. The hot-air dehydrated product which was treated with BHA showed little pigment loss in comparison with the pigment of the fresh surf clam meat. The BHA treatment upon the dehydrated surf clam products had an outstanding effect on the pigment retention and the EDTA treated one had a weak effect on it. The hot-air dehydrated products more rapidly re-absorbed water than the sun dried one. It could be seen that there was tittle difference among the rate of rehydration of the BHA or EDTA treated product and untreated one.

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