Effect of Ethylene Glycol (EG) and 1,2-Propanediol (PROH) on the Survival and the Development of Mouse and Human Embryos after Slow Freezing/Rapid Thawing Protocol
![]() |
Kim, Tae-Hyung
(Infertility Medical Center of CHA General Hospital, Pochon CHA University College of Medicine)
Cha, Soo-Kyung (Infertility Medical Center of CHA General Hospital, Pochon CHA University College of Medicine) Lee, Dong-Ryul (Infertility Medical Center of CHA General Hospital, Pochon CHA University College of Medicine) Han, Jee-Eun (Infertility Medical Center of CHA General Hospital, Pochon CHA University College of Medicine) Lee, Woo-Sik (Infertility Medical Center of CHA General Hospital, Pochon CHA University College of Medicine) Yoon, Tai-Ki (Infertility Medical Center of CHA General Hospital, Pochon CHA University College of Medicine) Cha, Kwang-Yul (Infertility Medical Center of CHA General Hospital, Pochon CHA University College of Medicine) Chung, Hyung-Min (Infertility Medical Center of CHA General Hospital, Pochon CHA University College of Medicine) |
1 | Lassalle B, Testart J, Renard JP. Human embryo features that influence the success of cryo-preservation with the use of 1,2-propanediol. Feril Steril 1985; 44: 645-51 DOI |
2 | Kasai M, Komi JH, Takakamo A, Tsudera H, Sakurai T, Machida T. A simple method for mouse embryo cryopreservation in a low toxicity vitrification solution, without appreciable loss of viability. J Reprod Fertil 1990; 89: 91-7 DOI PUBMED |
3 | Emiliani S, Bergh MV, Vannin A, Biramane J, Englert Y. Comparison of ethylene glycol, 1,2- propanediol and glycerol for cryopreservation of slow-cooled mouse zygotes, 4-cell embryos and blastocysts. Hum Reprod 2000; 15: 905-10 DOI ScienceOn |
4 | Newton H, Fisher J, Arnold JRP, Pegg DE, Faddy MJ, Gosden RG. Permeation of human ovrian tissue with cryoprotective agents in pre-paration for cryopreservation. Hum Reprod 1998; 13: 376-80 DOI ScienceOn |
5 | Guerif F, Bidault R, Cadoret V, Couet M, Lan-sac J, Royere D. Parameter guiding selection of best embryos for transfer after cryopreservation: a reappraisal. Hum Reprod 2002; 17: 1321-6 DOI ScienceOn |
6 | Paynter SJ, O'Neil L, Fuller BJ, Shaw RW. Membrane permeability of human oocytes in the presence of the cryoprotectant propane-1,2-diol. Fertil Steril 2001; 75: 532-8 DOI ScienceOn |
7 | Park SP, Kim EY, Kim DI, Park NH, Won YS, Yoon SH, et al. Simple, efficient and success-ful vitrification of bovine blastocyst using elec-tron microscope grids. Hum Reprod 1999; 14: 2838-43 DOI |
8 | Yoon TK, Chung HM, Lim JM, Han SY, Ko JJ, Cha KY. Pregnancy and delivery of healthy infants developed from vitrified oocytes in a sti-mulated in vitro fertiliation-embryo transfer pro-gram. Fertil Steril 2000; 74: 180-1 DOI ScienceOn |
9 | Mukaida T, Wada S, Takahashi K, Pedro PB, An TZ, Kasai M. Vitrification of human em-bryos based on the assessment of suitable condi-tions for 8-cell mouse embryos. Hum Reprod 1998; 13: 2874-9 DOI PUBMED ScienceOn |
10 | Kasai M, Nishimori M, Zhu SE, Sakurai T, Ma-chida T. Survival of mouse morulae vitrified in an ethylene glycol-based solution after exposure to the solution at various temperatures. Biol Re-prod 1992b; 47: 1134-9 DOI ScienceOn |
11 | Shaw JM, Ward C, Trounson AO. Evaluation of propanediol, ethylene glycol, sucrose and anti-freeze proteins on the survival of slow-cooled mouse pronuclear and 4-cell embryos. Hum Reprod 1995; 10: 396-402 DOI PUBMED |
12 | Zeilmaker GH, Alberta AT, van Gent I. Two pregnancies following transfer of intact frozen-thawed embryos. Fertil Steril 1984; 42: 293-26 DOI PUBMED |
13 | Burns WN, Gaudet TW, Martin MB, Ramiro Leal Y, Schoen H, Eddy CA, et al. Survival of cryopreservation and thawing with all blasto-meres intact indentifies multicell embryos with superior frozen embryo transfer outcome. Fertil Steril 1999; 72: 527-32 DOI ScienceOn |
14 | Chi HJ, Koo JJ, Kim MY, Joo JY, Chang SS, Chung KS. Cryopreservation of human embryos using ethylene glycol in controlled slow free-zing. Hum Reprod 2002; 17: 2146-51 DOI ScienceOn |
15 | Cho HJ. Son WY, Yoon SH, Lee SW, Lim JH. An improved protocol for dilution of cryopro-tectant from vitrified human blastocysts. Hum Reprod 2002; 9: 2419-22 |
16 | Ali J, Shelton N. Design of vitrification solution for the cryopreservation of embryos. J Reprod Fertil 1993; 99: 471-7 DOI PUBMED |
17 | Cocero MJ, Moreno S, Aguilar B. Ultrastructural characteristics of fresh and frozen-thawed bovine embryos using two cryoprotectants. Biol Reprod 2002; 66: 1244-58 DOI ScienceOn |
18 | Trounson A, Mohr L. Human pregnancy follo-wing cryopreservation, thawing and transfer of and eight-cell embryo. Nature 1983; 305: 707-9 DOI ScienceOn |
19 | Summerfeld V, Niemann H. Cryopreservation of bovine in vitro produced embryos using ethylene glycol in controlled freezing or vitrification. Cryo-biology 1999; 38: 95-105 |
![]() |