• 한국약용작물학회지
• /
• 제7권1호
• /
• pp.7-10
• /
• 1999

지황과 현삼을 대상으로 저장 온도와 기간이 직접 체세포배 발생에 미치는 영향을 조사하였다. 지황은 $4^{\circ}C$에서 저장한 후 배양한 경우보다 $8^{\circ}C$에서 저장하여 배양한 경우 직접 체세포배 생성율이 높았으며, 저장 조직 간에 차이가 있어, 잎이 12주간 저장 후에도 거의 일정한 체세포배 발생율을 보여 줄기나 엽병보다 장기 저장에 적합한 것으로 나타났다. 현삼의 각 조직을 전배양 없이 바로 $4^{\circ}C$와 $8^{\circ}C$에서 저장하였을 때, 줄기와 엽병 조직이 저장기간의 경과와 무관하게 신초의 발생율이 유지되었는데 경제적인 면으로 볼 때 $4^{\circ}C$ 조건보다는 $8^{\circ}C$ 조건이 적절할 것으로 판단되었다. 한편 현삼의 각 조직을 $25^{\circ}C$에서 10일 동안 전배양한 후에 저장하기 위해서는 줄기와 잎 조직은 $8^{\circ}C$에서, 엽병 조직은 $4^{\circ}C$ 조건에서 저장하는 것이 유리한 것으로 나타났다.

• 한국한의학연구원논문집
• /
• 제4권1호통권4호
• /
• pp.115-127
• /
• 1998

To improve the quality control of frequently used oriental medicinal plants, the storage, cutting, processing and decokting methods of Rehmannia glutinosa Libosch. var. purpurea Makino(熟地黃) were examined. The contents of 5-hydroxymethyl-2- furaldehyde(5-HMF) of Rehmannia glutinosa Libosch. var. purpurea Makino were analysed by HPLC at various conditions as the standard component. Raw materials were classified into 3 groups with their specific gravity(天 地 人黃) and determined the ding weights and the contents of water, the solid component of Ji-Hwang(地黃) was the most. Rehmannia glutinosa Libosch. var purpurea Makino was steamed and dried nine times and analysed the content of 5-HMF on every time. In the case of Ji-Hwang, the standard component of the ninth sample was produced the most. But which of the tenth samples of Chun-Hwang and Yin-Hwang(天黃 人黃) were the most. The best decokting conditions which were determined by the yield of 5-HMF were as follows : non cutting, soaking time was 0 minutes, decokting time was 75 minutes after boiling. The contents of sugar on each of samples were determined. The contents of standard component increased by steaming number and the contents of sugar decreased.

• Natural Product Sciences
• /
• 제16권2호
• /
• pp.116-122
• /
• 2010

A high-performance liquid chromatography (HPLC) with diode array detector (DAD) and electrospray ionization mass spectrometry (ESI-MS) was established for the simultaneous determination of five representative metabolites of the iridoid and phenolic classes from Rehmannia glutinosa. The optimal chromatographic conditions were obtained on an ODS column (5 mm, $4.6{\times}250\;mm$) with the column temperature at $25^{\circ}C$. The mobile phase was composed of water and acetonitrile using a gradient elution with the flow rate 0.3 mL/min. Detection wavelength was set at 205 nm. All calibration curves showed good linear regression ($r^2$ > 0.997) within test ranges. Limits of detection (LOD) and quantitation (LOQ) values were lower than 0.123 and $0.373\;{\mu}g/mL$, respectively. The developed method provided satisfactory precision and accuracy with overall intra-day and inter-day variations of 0.09 - 0.76% and 0.16 - 1.41%, respectively, and the overall recoveries of 99.03 - 102.67% for all of the compounds analyzed. In addition, effectiveness of diverse extraction methods was compared to each other for the development of standard analytic method. The verified method was successfully applied to the quantitative determination of five representative metabolites in twenty-one commercial Rehmannia glutinosa samples from different markets in Korea and China. The analytical results showed that the contents of the five analytes vary significantly with sources.

• 한국약용작물학회지
• /
• 제14권1호
• /
• pp.31-35
• /
• 2006

Agrobacterium rhizogenes ATCC15834에 의해 형질전환된 지황 (Rehmannia glutinosa)의 모상근으로부터 유식물체를 재분화 시키기 위한 최적 조건을 탐색하였다. 모상근 절편으로부터 유식물체의 재분화는 0.5 mg/l BA가 첨가된 SH배지 (3% sucrose, pH 5.8)에서 배양 5주 후 이루어졌다. 이와 같은 유식물체는 식물생장조절물질이 포함되지 않은 기본배지로 옮겼을 때 발근이 이루어졌다. 형질전환된 모상근 유래 식물체는 잎으로부터 직접 재분화된 식물체와 비교했을 때 잎이 오므라드는 경향을 보였고 줄기는 절간이 짧았으며, 뿌리는 가늘고 수량이 많은 특징을 보였다. 생체량 비교에서 형질전환식물체는 식물 하나의 무게가 1.28 g (생중량)으로 대조구인 잎 유래 재분화식물체 (0.53 g)에 비해 높았으며, 지표물질인 catalpol의 함량에 있어서도 차이를 보여주었다.

• 식물조직배양학회지
• /
• 제25권3호
• /
• pp.171-175
• /
• 1998

지황(Rehmannia glutinosa)의 캘러스배양을 통한 기내 대량증식을 위하여 엽조직으로부터 캘러스 형성에 미치는 생장조절물질의 영향을 조사한 결과 MS배지에 0.5 mg/L NAA와 2.0 mg/L BA 첨가배지에서 캘러스 형성율 100%, 식물체 분화율 31%로 가장 높았으며, 2.4-D와 zeatin 첨가배지에서는 캘러스 형성율은 95% 이상 높았으나 기관분화가 전혀 이루어지지 않았다. 재분화 식물체로부터 다수의 신초 유기에 적합한 배지로는 MS배지에 0.2mg/L NAA와 0.5 mg/L BA를 첨가한 배지에서 3.4개와 2.8개로 양호하였다. 다수의 신초 증식을 위하여 1.0 g/L활성탄을 첨가한 배지에서 가장 많은 3.8개의 신초가 분화되었으나 2.0 g/L 첨가배지에서는 2.4개로 감소된 반면, 초장과 엽수는 증가하여 개체생육은 양호함을 알 수 있었다.

• 한국의사학회지
• /
• 제34권2호
• /
• pp.89-96
• /
• 2021

This research aims to find a way to utilize the literature to select the herbs to be added or subtracted according to the condition of the patient after determining the basic prescription to be used for the patient. The study is based on data concerning the herbal composition and application of prescriptions described in the Donguibogam. The composition herbs was expressed as a weight ratio, the standard deviation of the weight ratio was calculated for each prescription, and the case where the weight ratio was greater than the base value (highest weight ratio - standard deviation) was designated as a principal herb. The various expressions of applications were changed to representative terms by the researcher and used to calculate the frequency. Of the 4,373 prescriptions for Donguibogam, 3,307 were able to be expressed in terms of weight ratios with 640 prescriptions using Rehmannia glutinosa, Cnidium officinale, Angelica gigas and Paeonia lactiflora as the principle herb. Of the 640 prescriptions, 508 prescriptions included applied information. Rehmannia glutinosa can also be used for symptoms such as fetal leakage(胎漏), short ingestion(食少), fever(發熱), dazed expression(眼花). Cnidium officinale can also be used for symptoms such as migraine(偏頭痛), stroke(中風癱瘓), tetanus(破傷風). Angelica gigas can also be used for symptoms such as forgetfulness(健忘), incontinence of urination(小便不通), palpitations(驚悸). Paeonia lactiflora can also be used for symptoms such as heart pain and abdominal pain(心腹痛), cough(咳嗽).

• 생명과학회지
• /
• 제25권11호
• /
• pp.1214-1222
• /
• 2015

본 연구는 난소 제거 수술을 시행하여 폐경기를 유도시킨 뒤 고지방 식이를 섭취한 쥐에게서 나타난 신체 변화에 있어 운동과 갈근/지황 섭취에 의한 개선 효과를 관찰하고, 그러한 효과가 골격근에서의 후성 유전적 발현 변화에 의한 것임을 규명하고자 하였다. 8주령의 쥐(rat, n=60)의 난소를 제거한 뒤 고지방 식이를 유도하면서 트레드밀 운동(exercise)을 실시하는 그룹과 비운동(sedentary) 그룹으로 나누었다. 두 그룹을 각각 estradiol, 갈근과 지황의 3:1 복합물(HT051), 그리고 물 섭취 군으로 다시 나누어 총 8주간 경구 투여를 함께 실시하였다. 그 결과 운동 그룹과 갈근/지황 섭취 그룹에서 체중이 유의하게 감소하였고, 가자미근과 족저근의 근질량 또한 운동 그룹과 갈근/지황 섭취 그룹에서 유의하게 증가하였다. 한편, 가자미근에서 물을 섭취하며 운동하지 않은 그룹의 H3K9 아세틸화가 억제 되었고 H3K9의 메틸화에는 변화가 없었다. 족저근의 경우 운동 그룹에서 H3K9 아세틸화가 현저하게 눈에 띄었고, 반대로 메틸화는 줄어든 것이 관찰되었다. 나아가 H3K9의 아세틸화와 메틸화를 조절하는 대표적인 효소 중 HDAC4, HDAC5, G9a 유전자의 mRNA 발현양을 정량한 결과, 가자미근에서는 모두 유의한 차이가 없었고 족저근에서 운동 한 그룹의 HDAC5와 G9a 유전자의 mRNA 발현양이 유의하게 감소하였지만 HDAC4의 mRNA는 차이가 없었다. 또한 운동과 갈근/지황의 상호작용 효과는 나타나지 않았다. 본 연구를 통하여 운동과 갈근/지황 섭취가 체중 감소, 근질량 증가에 영향을 미치고, 이러한 현상은 히스톤 H3K9 부분의 아세틸화와 메틸화에 의한 유전자 발현 조절이 그 기전으로 작용한다는 것을 알 수 있었다.

• BMB Reports
• /
• 제37권5호
• /
• pp.618-624
• /
• 2004

All members of R. glutinosa show the unique characteristic of intrinsic tolerance to paraquat (PQ). Antioxidant enzymes have been proposed to be the primary mechanism of PQ resistance in several plant species. Therefore, the antioxidant enzyme systems of R. glutinosa were evaluated by comparatively analyzing cellular antioxidant enzyme levels, and their responses of oxidative stresses and hormones. The levels of ascorbate peroxidase (APX), glutathione reductase (GR), non-specific peroxidase (POX), and superoxide dismutase (SOD) were 7.3-, 4.9-, 2.7- and 1.6-fold higher in PQ-tolerant R. glutinosa than in PQ-susceptible soybeans. However, the activity of catalase (CAT) was about 12-fold higher in the soybeans. The activities of antioxidant enzymes reduced after PQ treatment in the two species, with the exception of POX and SOD in R. glutinosa, which increased by about 40%. Interestingly, the activities of APX, SOD and POX in R. glutinosa, relative to those in soybeans, were further increased by 49, 67 and 93% after PQ treatment. The considerably higher intrinsic levels, and increases in the relative activities of antioxidant enzymes in R. glutinosa under oxidative stress support the possible role of these enzymes in the PQ tolerance of R. glutinosa. However, the relatively lower levels of SOD versus PQ tolerance, and the mixed responses of antioxidant enzymes to stresses and hormones, suggest a possible alternative mechanism(s) for PQ tolerance in R. glutinosa.

• International Journal of Oral Biology
• /
• 제32권1호
• /
• pp.23-34
• /
• 2007

We performed the present study to investigate whether Rehmannia glutinosa Libosch (RG) extracts (RGX) and Eleutherococcus senticosus Max (ES) extracts (ESX) play any roles in bone metabolism. We examined cellular activities of bone cells by measurement of osteoblastic cell viability, osteoprotegerin (OPG) secretion from osteoblasts, osteoclastogenesis, and osteoclastic activity. There is no cytotoxicity from osteoblasts after treatment with RGX and ESX. The secretion of OPG from the osteoblasts showed marked increases after treatment with RGX and ESX. In addition, RGX and ESX treatment decreased the number of tartrate-resistant acid phosphatase-positive multinucleated cells and the resorption areas. RGX and ESX, when mixed at optimal ratios, induced synergic effects, in vitro. OPB, which showed synergic effects, is the extract of natural ingredients RG and ES mixed at a raw material weight ratio of 4 : 1. It can be suspected that extracts of RG and ES mixtures contains active ingredients involved in bone tissue metabolism and may be effective in improving osteoporosis.

• 한방안이비인후피부과학회지
• /
• 제27권3호
• /
• pp.72-83
• /
• 2014

Objective: This study was to evaluate whether herbal mixture (HM-A : Houttuynia cordata Thunberg, Rubus coreanus, Rehmannia glutinosa, Prunus yedoensis, HM-B : Houttuynia cordata Thunberg, Rubus coreanus, Rehmannia glutinosa, Angelica gigas nakai) supresses the development of atopic dermatitis in Balb/c mice sensitized by ovalbumin. Methods: Mice were sensitized by intraperitoneal injection of ovalbumin plus aluminum hydroxide hydrate, followed by epicutaneous sensitization for 6 weeks. After induced atopic dermatitis, HM-A and HM-B were orally administrated for two weeks(once a two days) as a 50 mg/kg concentration. After all mice were sacrificed at the end of the experiment, skin and blood were harvested. Results: Oral administration group was reduced the infiltration of eosinophils, mast cells and total T cells on the skin areas as well as blood analysis. Also, cutaneous expression of IL-4,13,17 decreased. Blood IgE level was decreased. Conclusion: These drugs could be potential candidates for the atopic dermatitis.