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OPB, a water extract from Rehmannia glutinosa Libosch and Eleutherococcus senticosus Max, inhibits osteoclast differentiation and function  

Kim, Jung-Keun (Department of Pharmacology and Mechanism, Research Institute, Oscotec Inc.)
Kim, Se-Won (Department of Pharmacology and Mechanism, Research Institute, Oscotec Inc.)
Kim, Hae-Young (Department of Pharmacokinetics, Research Institute, Oscotec Inc.)
Lee, Byung-Eui (Department of Herbal medicine, Research Institute, Oscotec Inc.)
Ko, Seon-Yle (Department of Pharmacology and Mechanism, Research Institute, Oscotec Inc.)
Publication Information
International Journal of Oral Biology / v.32, no.1, 2007 , pp. 23-34 More about this Journal
Abstract
We performed the present study to investigate whether Rehmannia glutinosa Libosch (RG) extracts (RGX) and Eleutherococcus senticosus Max (ES) extracts (ESX) play any roles in bone metabolism. We examined cellular activities of bone cells by measurement of osteoblastic cell viability, osteoprotegerin (OPG) secretion from osteoblasts, osteoclastogenesis, and osteoclastic activity. There is no cytotoxicity from osteoblasts after treatment with RGX and ESX. The secretion of OPG from the osteoblasts showed marked increases after treatment with RGX and ESX. In addition, RGX and ESX treatment decreased the number of tartrate-resistant acid phosphatase-positive multinucleated cells and the resorption areas. RGX and ESX, when mixed at optimal ratios, induced synergic effects, in vitro. OPB, which showed synergic effects, is the extract of natural ingredients RG and ES mixed at a raw material weight ratio of 4 : 1. It can be suspected that extracts of RG and ES mixtures contains active ingredients involved in bone tissue metabolism and may be effective in improving osteoporosis.
Keywords
Rehmannia glutinosa Libosch; Eleutherococcus senticosus Max; bone metabolism; Osteoporosis;
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1 Awang, D.V.C.: Siberian ginseng toxicity may be a case of mistaken identity. Can. Med. Assoc. J. 155:1237, 1996
2 Baron, R., Neff, L., Van, P.T., Nefussi, J.R. and Vignery, A.: Kinetic and cytochemical identification of osteoclast precursors and their differentiation into multinucleated osteoclasts. Am. J. Pathol. 122:363-378, 1986
3 Heaney, R.P.: Calcium. in Principles of Bone biology. edited by Bilezikian, J.P., Raisz, L.G. and Rodan, G.A. pp 1007-1017, Academic Press, 1996
4 Kim, S.S., Son, Y.O., Chun, J.C., Kim, S.E., Chung, G.H., Hwang, K.J. and Lee, J.C.: Antioxidant property of an active component purified from the leaves of paraquat-tolerant Rehmannia glutinosa. Redox. Rep. 10:311-318, 2005   DOI   ScienceOn
5 Martin, J.H. and Matthews, J.L.: Mitochondrial granules in chondrocytes, osteoblasts and osteocytes. An ultrastructural and microincineration study. Clin. Orthop. 68:273-278, 1970
6 Minkin, C.: Bone acid phosphatase: Tartrate-resistant acid phosphatase as a marker of osteoclast function. Calcif. Tissue Int. 34:285-290, 1982   DOI
7 Mundy, G.R. and Roodman, G.D.: Osteoclast ontogeny and function, in Bone and Mineral Research, vol. 5, edited by Peck, W.A., pp 209-279, Elsevier, Amsterdam, 1987
8 Pixley, F.J. and Stanley, E.R.: CSF-1 regulation of the wandering macrophage: Complexity in action. Trends Cell Biol. 14:628-638, 2004   DOI   ScienceOn
9 Reginster, J.Y.: Treatment of bone in elderly subjects: calcium, vitamin D, fluor, bisphosphonates, calcitonin. Horm. Res. 43:83-88, 1995   DOI
10 Sasaki, T., Takahashi, N., Higashi, S. and Suda, T. Multinucleated cells formed on calcified dentin from mouse bone marrow cells treated with 1a,25-dihydroxyvitamin $D_{3}$ have ruffled borders and resorb dentin. Anat. Rec. 224:379- 391, 1989   DOI   ScienceOn
11 Suda, T., Takahashi, N., Udagawa, N., Jimi, E., Gillespie, M.T. and Martin, T.J.: Modulation of osteoclast differentiation and function by the new members of the tumor necrosis factor receptor and ligand families. Endocr. Rev. 20:345-57, 1999   DOI   ScienceOn
12 Wasnich, R.: What is an osteoporotic fracture? in Osteoporosis: Diagnotic and Therapeutic Principles, edited by Rosen, C.J., pp 79-88, Humana Press, Totowa, NJ, 1996
13 Recker, R.P.: Current therapy for osteoporosis. J. Clin. Endocrinol. Metab. 76:14-16, 1993   DOI
14 Chang, H.M. and But, P.P.H.: Pharmacology and applications of Chinese amteria medica. vol 1, edited by Yao, S.C., Wang, L.L. and Shem, C.S. p 461, 1986
15 Rodan, G.A.: Emerging therapies in osteoporosis. Ann. Rep. Med. Chem. 29:275-285, 1994   DOI
16 Wang, J.Z., Tsumura, H., Shimura, K. and Ito, H.: Antitumor activity of polysaccharide form a Chinese medicinal herb, Acnthopanax giraldii Harms. Cancer Lett. 65:79-84, 1992   DOI   ScienceOn
17 Fleisch, H.: Bisphosphonates: Mechanism of action and clinical use. in Principles of Bone biology. edited by Bilezikian, J.P., Raisz, L.G. and Rodan, G.A. pp 1037-1052, Academic Press, 1996
18 Scheven, B.A.A., Visser, J.W.M. and Nijweide, P.J.: In vitro osteoclast generation from different bone marrow fractions, including a highly enriched hematopoietic stem cell population. Nature 321:79-81, 1986   DOI   ScienceOn
19 Canalis, E.: Effect of growth factors on bone cell replication and differentiation. Clin. Orthop. Rel. Res. 193:246-263, 1985
20 Raju, B.L., Lin, S.J., Hou, W.C., Lai, Z.Y., Liu P.C. and Hsu, F.L.: Antioxidant iridoid glucosides from Wendlandia formosana, Natural Product Res. 8:357-364, 2004
21 Ben-Hur, E. and Fulder, S.: Effect of Panax ginseng saponins and Eleutherococcus senticosus on survival of cultured mammalian cells after ionizing radiation. Am. J. Chin. Med. 9:48-56, 1981   DOI   ScienceOn
22 Chao, J.C., Chiang, S.W., Wang, C.C., Tsai, Y.H. and Wu, M.S.: Hot water-extracted Lycium barbarum and Rehmannia glutinosa inhibit proliferation and induce apoptosis of hepatocellular carcinoma cells. World J. Gastroenterol. 12:4478-4484, 2006   DOI
23 Jin, U.H., Kim, D.I., Lee, T.K., Lee, D.N., Kim, J.K., Lee, I.S. and Kim, C.H.: Herbal formulation, Yukmi-jihang-tang- Jahage, regulates bone resorption by inhibition of phosphorylation mediated by tyrosine kinase Src and cyclooxygenase expression. J. Ethnopharmacol. 106:333- 343, 2006   DOI   ScienceOn
24 Nelson, H.D., Humphrey, L.L., Nygren, P., Teutsch, S.M. and Allan, J.D.: Postmenopausal hormone replacement therapy: scientific review. JAMA 288:872-881, 2002   DOI   ScienceOn
25 Shan, B.E., Yoshita, Y., Sugiura, T. and Yamashita, U.: Suppressive effect of Chinese medicinal herb, Acanthopanax gracilistylus, extract on human lympocytes in vitro. Clin. Exp. Immunol.118:41-48, 1999   DOI
26 Takasugi, N., Moriguchi, T., Fuwa, T., Sanada, S., Ida, Y., Shofi, J. and Saito, H.: Effect of Eleutherococcus senticosus and its rectal temperature, body and grip tones, mortor coordination, and exploratory and spontaneous movements in acute stressed mice. Shoyakugaku Zasshi. 39:232-237, 1985
27 Tang, W. and Eisenbrand, G.: Chinese Drugs of Plant Origin: in Chemistry, Pharmacolgy, and Use in Traditional Modern Medicine, pp 849-854, Springer-Verlag, Berlin 1992
28 Kormosh, N., Laktionov, K. and Antoshechkina, M.: Effect of a combination of extract from several plants on cellmediated and humoral immunity of patients with advanced ovarian cancer. Phytother. Res. 20:424-425, 2006   DOI   ScienceOn
29 生藥學硏究會 : 現代生藥學, PP263-265, 학창사, 서울 1998
30 Fujikawa, T., Yamaguchi, A., Morita, I., Takeda, H. and Nishibe, S.: Protective effects of Acanthopanax senticosus Harms from Hokkaido and its components on gastric ulcer inrestrained cold water stressed rats. Biol. Pharm. Bull. 19:1227-1230, 1996   DOI
31 Kostenuik, P.J. and Shalhoub, V: Osteoprotegerin: A physiological and pharmacological inhibitor of bone resorption. Curr. Pharm. Des. 7:613-635, 2001   DOI   ScienceOn
32 Owen, M.E.: Lineage of osteogenic cells and their relationship to the stromal system. in Bone and Mineral Research, edited by Peck, W.A., pp 1-25, Elsevier Science Publisher, Amsterdam, 1985
33 Raisz, L.G.: Hormonal regulation of bone growth and remodeling. in Cell and Molecular Biology of Vertebrate Hard Tissue CIBA Foundation Symposium 136, pp 226- 238, Wiley, NY, 1988
34 Boyle, W.J., Simonet, W.S. and Lacey. D.L.: Osteoclast differentiation and activation. Nature 423:337-342, 2003   DOI   ScienceOn
35 Takahashi, N., Yamana, H., Yoshiki, S., Roodman, G.D., Mundy, G.R., Jones, S.J., Boyde, A. and Suda, T.: Osteoclast-like cell formation and its regulation by osteotropic hormones in mouse bone marrow cultures. Endocrinol. 122:1373-1382, 1988a   DOI   ScienceOn
36 Nijweide, P.J., Burger, E.H. and Feyen, J.H.M.: Cells of bone: Proliferation, differentiation, and hormonal regulation. Physiol. Rev. 66:855-886, 1986   DOI
37 Shen, M.L., Zhai, S.K., Chen, H.L., Luo, Y.D., Tu, G.R. and Ou, D.W.: Immunopharmacological effects of polysaccharides from Acanthopanax senticosus on experimental animals. Int. J. Immunopharmacol. 13:549- 554, 1991   DOI   ScienceOn
38 Sridhar, C., Subbaraju, G.V., Venkateswarlu, Y. and Venugopal, R.T.: New acylated iridoid glucosides from Products 67:Vitex altissima. J. Natural 2012-2016, 2004   DOI   ScienceOn
39 Yu, H.H., Seo, S.J., Kim, Y.H., Lee, H.Y., Park, R.K., So, H.S., Jang, S.L. and You, Y.O.: Protective effect of Rehmannia glutinosa on the cisplatin-induced damage of HEI-OC1 auditory cells through scavenging free radicals. J. Ethnopharmacol. 107:383-388, 2006   DOI   ScienceOn
40 Zhao, Y., Wen, X.S., Cui, J. and Wu, W.H.: Extraction and salting-out purification of alpha-galactosidase and betaglucosidase from fresh roots of Rehmannia glutinosa. Zhong. Yao. Cai. 29:137-139, 2006
41 Canalis, E., McCarthy, T. and Centrella, M.: Growth factors and the regulation of bone remodeling. J. Clin. Invest. 81:277-281, 1988   DOI   ScienceOn
42 Stock, J.L.: Drug therapy. in Osteoporosis: Diagnotic and Therapeutic Principles, edited by Rosen, C.J., pp 173-187, Humana Press, Totowa, NJ, 1996
43 Takahashi, N., Akastu, T., Sasaki, T., Nicholson, G.C., Moseley, J.M., Martin, T.J. and Suda, T.: Induction of calcitonin receptors by 1a,25-dihydroxyvitamin $D_{3}$ in osteoclast-like multinucleated cells formed from mouse bone marrow cells. Endocrinol. 123:1504-1510, 1988b   DOI   ScienceOn
44 Wang, J.Z., Mao, X.J., Ito, H. and Shimura, K.: Immunomodulatory activity of polysaccharide from Acanthopanax obovatus roots. Planta. Med. 57:335-336, 1991   DOI   ScienceOn