• Korean Journal of Medicinal Crop Science
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• v.12 no.2
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• pp.171-178
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• 2004

The objectives of this study were to establish the genetic transformation system of stilbene synthase in Rehmannia glutinosa. Resveratrol, which is both a phytoalexin with antifungal activity and a phytochemical associated with reduced cancer risk and reduced cardiovascular disease, is synthesized in a limited number of plant species including peanut. Resveratrol synthesis is catalyzed by the enzyme stilbene synthase including resveratrol synthase (RS). Stilbene synthase gene (RS3) obtained from peanut, Arachis hypogaea, Fabaceae has been transferred into chinese foxglove, Rehmannia glutinosa by using Agrobacterium mediated transformation. PCR analysis with RS3 primer confirmed that the targeted gene was introduced into the plant genome, 904 bp in size. Further analyses of identification of transformation using developed other molecular techniques and transgenic plants that RS t-DNA introduced to chinese foxglove (R. glutinosa L) and its reaction product, stilbene such as resveratrol will be isolate and characterize using NMR, MS, and HPLC.

• Korean Journal of Plant Resources
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• v.15 no.1
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• pp.72-76
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• 2002

This study was carried out to determine an effective mechanical harvester for rhizomes of Rehmannia glutinosa Libosch. Labor-saving efficiency showed 69∼76% by using tillage operations with power tiller, digger attached to power tiller, and digger attached to tractor compared with manual harvest. Loss percentage of rhizomes by mechanical harvesters was 3.1∼ 9.3% higher, and fresh rhizome yield was 2∼6％ lower than that by manual harvest, respectively. Although it showed higher loss percentage of rhizomes and lower fresh rhizome yield compared with manual harvest, mechanical harvest using digger attached to power tiller or digger attached to tractor was effective in labor-saving for rhizomes of Rehmannia glutinosa Libosch.

• The Journal of Korean Medicine
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• v.17 no.1 s.31
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• pp.329-335
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• 1996

Rehmannia Radix is a thick tuberous root about 3-6 cm diameter. The tuberous root is repared for medicine. The material in the fresh state is shengdihuang (生地黃). The material in the dried state is gandihuang (乾地黃). Shudihuang (熱地黃) is made by taking juicy roots, washing them in millet wine, steaming on a willow frame in a percelain vessel, drying, and resteaming and redrying nine times. The aim of this experiments was to investigate the effect of Rehmannia Radix Water extracts on the plasma renin activity and plasma levels of aldosterone and atrial natriuretic peptide (ANP) in rats. The results of study were as follows: Plasma renin activity was not changed after administration of Rehmannia Radix water extracts. Plasma levels of aldosterone was decreased significantly after administration of Shudihuang (熱地黃) water extracts. Plasma levels of atrial natriuretic peptide was increased significantly after administration of Shudihuang (熱地黃) water extracts.

• The Korea Journal of Herbology
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• v.21 no.2
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• pp.9-13
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• 2006

Objectives : To determine the DNA sequence of the 18S rRNA gene of the Rehmannia glutinosa and analyze it phylogenetically Methods : Dried root of the Rehmannia glutinosa was ground with a mortar and pestle. Glass beads(0.5 mm in diameter), TE buffer and SDS solution were added to that. The mixture was vortexed vigorously and extracted with the mixture of phenol, chloroform and isoamyl alcohol and with the mixture of the chloroform and isoamyl alcohol. The nucleic acids were precipitated with ethanol and resuspended in TE buffer. Contaminating RNA was digested with RNAse A and the DNA was purified further with the Geneclean Turbo Kit. This DNA was used as a template for amplification of the 18S rRNA gene by PCR. The PCR product was cloned in the pBluescript SK II plasmid by blunt-end ligation and the DNA sequence of the insert was determined. This DNA sequence was analyzed phylogenetically by the BLAST program. Results and Conclusion : Vortexing the ground powder of the dried plant root with glass beads during cell lysis improved recovery of DNA. The DNA sequence of the Rehmannia glutinosa 18S rRNA gene was determined and deposited at the GenBank as the accession number DQ469606. Phylogenetic analysis of that sequence showed the relationship between the members of the family of Scrophulariaceae and also the close relationship of the Buddleja davidii to the members of the Scrophulariaceae family.

• Korean Journal of Medicinal Crop Science
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• v.6 no.3
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• pp.221-226
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• 1998

This study was conducted to develop labor-saving cultural techniques for weed control using herbicides in Rehmannia glutinosa and Astragalus membranaceus, Napropamide, linuron(preemergence) and paraquat (postemergence) showed significant herbicidal effects. At the same time they did not turn out to be harmful to the growth of Rehmannia glutinosa. The yield of Rehmannia, glutinosa treated with paraquat was higher than that of the untreated. Napropamide showed significant herbicidal effect without harmful effects to the growth of Astragalus membranaceus. Etha1flura1in and metolachlor, however, were s1ightly harmful. The yield of Astragalus membranaceus was highest with napropamide.

• Korean Journal of Pharmacognosy
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• v.25 no.1
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• pp.28-30
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• 1994

The rate of growth and production of bioactive substances from Rehmannia glutinosa Liboschitz (Scrophulariaceae) were studied with the variation on the constituents of the culture media. The best growth was observed from MS basal medium containing 3.0 ppm NAA and 2.0 ppm kinetin. Carbohydrates (fructose, glucose and sucrose), phytosterols(${\beta}-sitosterol$, campesterol and stigmasterol) and carotenoid like substances were identified by GC-MS and TLC from the callus mass. However, catalpol was not detected from both solid and cell suspension cultures containing geraniol. Callus cultured Rehmannia glutinosa in the MS basal medium containing 0.1 ppm NAA and 0.1 ppm kinetin become differentiated to root.

• Food Science and Biotechnology
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• v.18 no.1
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• pp.202-206
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• 2009

Drying characteristics of fresh Korean-type rehmannia (jiwhang) noodle was investigated to determine drying kinetic parameters under the experimental conditions of 5 temperatures (30, 40, 60, 80, and $90^{\circ}C$). Drying curve of the noodle showed a biphasic pattern of decrease in drying rate with initial rapid drying followed by slow dehydration as the progress in drying. In all drying conditions, only falling drying rate period was observed and the drying rate of the noodle was greatly influenced by the drying temperature. The effective diffusion coefficients ($D_{eff}$) were determined by the diffusion model and their temperature dependency was determined using an Arrhenius equation. The activation energy ($E_a$) values for the drying of the noodle were 19.94 and 21.09 kJ/mol at the initial and the latter stage of dehydration, which were comparable to those of pasta or Japanese udong dehydration.

• Mycobiology
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• v.30 no.2
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• pp.88-92
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• 2002

Rehmannia glutinosa is a perennial medicinal plant belonging to the family Scrophulariaceae with more than 300 species known in the world, especially in temperate regions. Its roots have been used widely in Korea for medicinal purposes. However, it is commonly infected by various pathogens during storage, causing great damage to the roots, and impedes the intensive farming of the crop. Therefore, an attempt has been made to isolate and screen a resistance gene against the pathogen Fusarium oxysporum using differential display. We treated salicylic acid(SA), and isolated a resistance gene that responds to SA. As a result, we found that SA was involved in plant defense mechanism in pathogenicity tests with SA treated and non-treted plants, and we isolated a partial PR-la gene through differential display polymerase chain reaction(DD-PCR) method.

• Korean Journal of Medicinal Crop Science
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• v.7 no.2
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• pp.138-142
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• 1999

Chinese foxglove (Rehmannia glutinosa) is receiving much attention as one of the principal medicinal crops and the demand for crude drug expands rapidly. This study was conducted to obtain the basic agronomic characteristics and cultivation information of Chinese foxglove. Morphological traits of several Chinese foxglove and their plant growth and yield were investigated under different environmental conditions. The tested lines exhibited clear morphological differences in leaves and roots representing their origins. Rapid root growth and weight increasement occurred in the middle of July. Optimum daylength and temperature conditions were investigated for the adequate plant growth of Chinese foxglove. Root growth was enhanced at $23/18^{\circ}C$ (day/night) with 13 hours daylength condition. Appropriate soil moisture and soil texture were $60{\sim}70%$ and loam soil, respectively.

• Journal of agriculture & life science
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• v.50 no.5
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• pp.81-93
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• 2016

Rehmannia glutinosa(Gaertn.) DC. is a herbaceous perennial plant and belonging to the Scrophulariaceae and used as roots for medicinal part and purpose. R. glutinosa is and usually used for fresh rehmannia root or prepared rehmannia root. However, it is very difficult to propagate using the seeds because of lack germination so it is propagated using the vegetative method as the rootstock. Currently, propagation and harvesting using the rootstock of R. glutinosa has difficulties about production of the high quality and quantity in R. glutinosa because of root rot disease. To optimize in vitro cultures and to improve the rootstock and seedling of R. glutinosa after morphological and genetical determination, 5 plant culture media (MS, DJ, LS, QL, and WPM) were used in this study then WPM was selected for better growth, for multiplication condition(WPM + IAA 1.0 mg/L + IBA 0.5 mg/L), and for root enlargement condition(WPM + NAA 0.1 mg/L) of R. glutinosa. Based on these results, in vitro seedlings of R. glutinosa were transferred to soil for acclimation with environment adaptation and shown the positive effects about root enlargement and root formation. Therefore, it can be used for high quality of R. glutinosa production and production of the rootstock based on propagation using in vitro seedlings of R. glutinosa.