• Journal of Life Science
• /
• v.11 no.1
• /
• pp.62-69
• /
• 2001

This study was aimed to investigate the effect Chukdamtanggamibang on the vascular systems including changes in blood pressure and regional cerebral blood flow(rCBF) of male Sprague-Dawely rats, contractile force of guinea pig`s tracheal smooth muscle and abdominal aorta and femoral artery in rabbits. Blood pressure was not affected by Chukdamtanggamibang in rats. rCBF was significantly increased by Chukdamtanggamibang in a dose-dependent manner. Contractile force of isolated guinea pig`s tracheal smooth muscle evoked by His ({TEX}$ED_{50}${/TEX}) were inhibited significantly by Chukdamtanggamibang. Propranolol, indomethacin and methylene blue did not significantly alter the inhibitory effect of Chukdamtanggamibang. Contractile force of isolated rabbit`s abdominal aorta and femoral artery evoked by NE ({TEX}$ED_{50}${/TEX}) were inhibited significantly by Chukdamtanggamibang. ODQ and L-NNA significantly attenuated the inhibitory effects of Chukdamtanggamibang in abdominal aorta, whereas propranolol did not significantly alter the inhibitory effect of Chukdamtanggamibang. These results indicate that Chukdamtanggamibang can relax hitamine-induced contraction of guinea pig`s tracheal smooth muscle and that this inhibition involves, in part, the relation to the soluble guanylyl cyclase synthesis and nitric oxide (NO) synthesis.

• Journal of Acupuncture Research
• /
• v.27 no.3
• /
• pp.47-57
• /
• 2010

Experimental Study of Taegye($KI_3$) Taebaek($SP_3$) Draining Acupuncture on the Improvement of Cerebral Blood flow and Arterial Blood Pressure. Objectives : This study was designed to investigate the effects of acupuncture on Taegye($KI_3$) Taebaek($SP_3$) draining and determine the mechanism of action of PCBL by measuring the changes of regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in normal rats. Methods : This study was designed to investigate whether acupuncture on Taegye($KI_3$) Taebaek($SP_3$) draining affect Regional cerebral blood flow(rCBF), Mean arterial blood pressure(MABP) in normal rats. To make manifest whether acupuncture on Taegye($KI_3$) Taebaek($SP_3$) draining was mediated by inhibitor of cyclooxygenase or guanylate cyclase which diate arterial diameter. Results 1. Acupuncture on Taegye($KI_3$) Taebaek($SP_3$) draining significantly increased rCBF but decreased MABP. This result suggests that Acupuncturing on Taegye($KI_3$) Taebaek($SP_3$) draining might significantly increase rCBF by dilating arterial diameter. 2. Acupuncture on Taegye($KI_3$) Taebaek($SP_3$) draining induced increase of rCBF was significantly inhibited by pretreatment with indomethacin (1 mg/kg, i.p.), an inhibitor of cyclooxygenase. 3. This result suggests that the action of Acupuncture on Taegye($KI_3$) Taebaek($SP_3$) draining might be mediated by cyclooxygenase.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.16 no.1
• /
• pp.99-103
• /
• 2002

Geopungdodam-tang(GDT) have been used in oriental medicine for many centuries as a therapeutic agent of apoplexy. The mechanism of GDT on the cerebral blood flow is not known. The purpose of this Study was to investigate effects of GDT on the pial arterial diameter and action mechanism of GDT-induced increased regional cerebral blood flow(rCBF). The changes of regional cerebral blood f1ow(rCBF) was determinated by Laser-Doppler Flowmetry(LDF), and the changes of pial arterial diametet were determinated by video microscopy methods and video analyzer. The results were as follows ; 1. Pial arterial diameter was significantly increased by GDT in a dose-dependent manner. 2. Pretreatment with L-NNA significantly inhibited GDT induced increased rCBF. 3. Pretreatment with methylene blue significantly inhibited GDT induced increased rCBF. 4. Pretreatment with indomethacin inhibited GDT induced increased rCBF. These results suggest that GDT causes a diverse response of cerebral hemodynamics(rCBF and pial arterial diameter). The cerebral hemodynamics is also mediated by nitric oxide synthase, cyclic GMP(guanylyl cydase) and prostaglandin(cyclooxygenase).

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.21 no.1
• /
• pp.54-61
• /
• 2007

The study was designed to investigate the effects of Mixture of Yukgunja-tang and Bambusae Caulis in Liquamen (YTBCL) on the change of regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in rats, and further to determine the mechanism of action of YTBCL. The results in rats were as follows ; YTBCL 25 ${\mu}l$ significantly decreased rCBF and MABP compared with basal condition. YTBCL 100 ${\mu}l$ significantly increased rCBF compared with basal condition, but decreased MABP compared with basal condition. YTBCL 50 ${\mu}l$ significantly increased rCBF compared with basal condition, but MABP was somewhat decreased compared with basal condition. The VTBCL 50 ${\mu}l$-induced increase in rCBF was significantly inhibited by pretreatment with methylene blue (10 ${\mu}g$/kg, i.p.), an inhibitor of guanylate cyclase and indomethacin (1 mg/kg, i.p.), an inhibitor of cyclooxygenase. The YTBCL 50 ${\mu}l$-induced decreased MABP significantly increased by pretreatment with methylene blue but was inhibited by indomethacin. This results were suggested that the mechanism of YTBCL was mediated Dy guanylate cyclase.

• The Journal of Internal Korean Medicine
• /
• v.30 no.2
• /
• pp.298-305
• /
• 2009

Objectives: The present study was carried out to investigate the effects of Yupoongyangyeong-tang(YYT) on changes of regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in rats. In addition, the author also investigated action mechanisms of YYT on changes in rCBF and MABP. Methods and Results : In this study, treatment with YYT elevated rCBF in a dose-dependent manner, but MABP levels were elevated only in the 10 mg/ml treatment group. Pre-treatment with indomethacin. an inhibitor of cyclooxygenase, inhibited increase of rCBF effectively. Pre-treatment with methylene blue, an inhibitor of guanylate cyclase, inhibited increase of rCBF induced by YYT, too. In addition. pre-treatment with indomethacin also inhibited increase of MABP. However, pre-treatment with methylene blue did not affect MABP levels. Conclusion : These results suggest that YYT is useful to treat patients with diseases related to cerebral ischemia, because YYT can increase rCBF. In addition, the mechanisms are thought to be related to both cyclooxygenase and guanylate cyclase.

• Herbal Formula Science
• /
• v.10 no.1
• /
• pp.93-103
• /
• 2002

This experimental studies were done to investigate the effects of Sambutang(SBT) and consitituent herbs on the regional cerebral blood flow(rCBF) and mean arterial blood pressure(BP) in rats. The results are as follows: SBT and consitituent herbs(SBT omitting Zingiberis Rhizoma Recens, Zingiberis Rhizoma Recens. Ginseng Radix and Aconiti iateralis preparata Radix) was increased rCBF significantly in a dose-dependent manner. SBT omitting Zingiberis Rhizoma Recens and Ginseng Radix were decreased significantly BP in rats. But, SBT, Aconiti iateralis preparata Radix and Zingiberis Rhizoma Recens were not affected BP in rats. According to the above findings. it is suggested that SBT causes a diverse response of rCBF, and specially the rCBF by increasing SBT is mediated action of Zingiberis Rhizoma Recens.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.21 no.6
• /
• pp.1394-1400
• /
• 2007

This study was designed to investigate the effects of Samul-tang Extract and Bambusae caulis in liquamen(SEB) on the changes in regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in normal rats. The results were as follows ; SBE significantly increased rCBF in a dose-dependent manner, and increased MABP in a dose-dependent manner. This result suggests that SBE significantly increased rCBF by dilating pial arterial diameter. Increase of SBE-induced rCBF was significantly inhibited by pretreatment with indomethacin (1 mg/kg, i.p.), an inhibitor of cyclooxygenase and increase of SBE-induced rCBF was inhibited by pretreatment with methylene blue ($10\;{\mu}g/kg$, i.p.), an inhibitor of guanylate cyclase. Increase of SBE-induced MABP was significantly increased by pretreatment with methylene blue but increase of SBE-induced MABP was decreased by pretreatment with indomethacin. These results suggested that the action of SBE was mediated by cyclooxygenase.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.19 no.6
• /
• pp.1599-1603
• /
• 2005

The study was designed to investigate the effects of Leonuri herba extract (LHE) on the change of regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in normal rats, and further to determine the mechanism of action of LHE. The results in normal rats were as follows, LHE significantly increased rCBF in a dose-dependent manner, and MABP did not change in a dose-dependent manner. This results were suggested that LHE significantly increased rCBF by dilating pial arterial diameter. The LHE-induced increase in rCBF was significantly inhibited by Pretreatment with indomethacin(1 mg/kg, i.p.), an inhibitor of cyclooxygenase, and was significantly inhibited by methylene blue($10{\mu}g/kg$, i.p.), an inhibitor of guanylate cyclase. The LHE-induced MABP did not change by pretreatment with indomethacin but was significantly inhibited by methylene blue. This results were suggested that the mechanism of LHE was mediated by cyclooxygenase.

• Journal of Physiology & Pathology in Korean Medicine
• /
• v.20 no.6
• /
• pp.1538-1542
• /
• 2006

The study was designed to investigate the effects of MokhwyangJoki-san Extract (MJSE) on the change of regional cerebral blood flow (rCBF) and mean arterial blood pressure (MABP) in normal rats, and further to determine the mechanism of MJSE. The results in normal rats were as follows ; MJSE significantly increased rCBF in a dose-dependent manner, and MABP did not change in a dose-dependent manner. This results were suggested that MJSE significantly increased rCBF by dilating pial arterial diameter. The MJSE-induced increase in rCBF was significantly inhibited by pretreatment with methylene blue (10 ${\mu}g/kg$, i.p.), an inhibitor of guanylate cyclase, and was not changed by indomethacin (1 ${\mu}g/kg$, i.p.), an inhibitro of cyclooxygenase. The MJSE-was not changed MABP was decreased by pretreatment with indomethacin but was not changed by methylene blue. This results were suggested that the mechanism of MJSE was mediated by guanylate cyclase.

• The Korean Journal of Nuclear Medicine
• /
• v.29 no.1
• /
• pp.48-53
• /
• 1995

This study investigates a simple mathematical model for the quantitative estimation of regional myocardial blood flow in experimental canine coronary artery thrombosis using Rb-82 dynamic myocardial positron emission tomography. The coronary thrombosis was induced using the new catheter technique by narrowing the lumen of coronary vessel gradually, which finally led to partial obstruction of coronary artery. Ten Rb-82 dynamic myocardial PET scans were performed sequentially for each experiment using our 5, 10 and 20 second acquisition protocol, respectively, and three regions of interest were drawn on the transaxial slices, one on left ventricular chamber for input function and the other two on normal and decreased perfusion segments for the flow estimation in those regions. Single compartment model has been applied to the measured sets of regional PET data, and the rate constants of influx to myocardial tissue were calculated for regional myocardial flow estimates with the three parameter fits of raw data by the Levenberg-Marquardt method. The results showed that, (1) single compartment model suggested by Kety-Schmidt could be used for the simple estimation of regional myocardial blood flow, (2) the calculated regional myocardial blood flow estimates were dependent on the selection of input function, which reflected partial volume effect and left ventricular wall motion, and (3) mathematically fitted input and tissue time activity curves were more suitable than the direct application of the measured data in terms of convergence.