• Ocean and Polar Research
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• 제40권4호
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• pp.289-297
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• 2018

This study reconstructs past vegetation changes in southeastern Korea over the last 30 thousand years using plant waxes (i.e. long chain n-alkanes) and their carbon isotopic compositions (${\delta}^{13}C_{alk}$) preserved in marine sediment core (KIODP 12-1) retrieved from the East Sea. Here we show changes in vegetation composition in the Korean peninsula in relation to the strength of the East Asian Summer Monsoon. During the Last Glacial Maximum (LGM), when the summer monsoon weakened, precipitation decreased and $C_3$ grassland expanded. After the LGM, the summer monsoon gradually intensified, increasing rainfall, and thus expanding the forestland coverage. Precipitation climaxed from 10 to 6 kyr BP, which includes the Holocene Climate Optimum. The grassland began to expand since 5 kyr BP due to climate warming and drying towards the present. The ${\delta}^{13}C_{alk}$ values may also have been influenced by agricultural activities, which is known to have begun since the late Neolithic (ca. 7.0~3.0 kyr BP). Our results demonstrate how changes in the global climate state influence regional atmospheric circulation and precipitation distribution, and consequently terrestrial plant composition in southeastern Korea.

• Journal of Microbiology and Biotechnology
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• 제31권10호
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• pp.1393-1400
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• 2021

Acetone-butanol-ethanol (ABE) fermentation by the anaerobic bacterium Clostridium acetobutylicum has been considered a promising process of industrial biofuel production. Phosphotransbutyrylase (phosphate butyryltransferase, PTB) plays a crucial role in butyrate metabolism by catalyzing the reversible conversion of butyryl-CoA into butyryl phosphate. Here, we report the crystal structure of PTB from the Clostridial host for ABE fermentation, C. acetobutylicum, (CaPTB) at a 2.9 Å resolution. The overall structure of the CaPTB monomer is quite similar to those of other acyltransferases, with some regional structural differences. The monomeric structure of CaPTB consists of two distinct domains, the N- and C-terminal domains. The active site cleft was formed at the interface between the two domains. Interestingly, the crystal structure of CaPTB contained eight molecules per asymmetric unit, forming an octamer, and the size-exclusion chromatography experiment also suggested that the enzyme exists as an octamer in solution. The structural analysis of CaPTB identifies the substrate binding mode of the enzyme and comparisons with other acyltransferase structures lead us to speculate that the enzyme undergoes a conformational change upon binding of its substrate.

• 한국축산식품학회지
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• 제41권5호
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• pp.883-893
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• 2021

Clostridium difficile present in feces of food animals may contaminate their meats and act as a potential source of C. difficile infection (CDI) to humans. C. difficile resistance to antibiotics, its production of toxins and spores play major roles in the pathogenesis of CDI. This is the first study to evaluate C. difficile prevalence in retail raw animal meats, its antibiotics susceptibilities and toxigenic activities in Al-Jouf, Saudi Arabia. Totally, 240 meat samples were tested. C. difficile was identified by standard microbiological and biochemical methods. Vitek-2 compact system confirmed C. difficile isolates were 15/240 (6.3%). Toxins A/B were not detected by Xpect C. difficile toxin A/B tests. Although all isolates were susceptible to vancomycin and metronidazole, variable degrees of reduced susceptibilities to moxifloxacin, clindamycin or tetracycline antibiotics were detected by Epsilon tests. C. difficile strains with reduced susceptibility to antibiotics should be investigated. Variability between the worldwide reported C. difficile contamination levels could be due to absence of a gold standard procedure for its isolation. Establishment of a unified testing algorithm for C. difficile detection in food products is definitely essential to evaluate the inter-regional variation in its prevalence on national and international levels. Proper use of antimicrobials during animal husbandry is crucial to control the selective drug pressure on C. difficile strains associated with food animals. Investigating the protective or pathogenic potential of non-toxigenic C. difficile strains and the possibility of gene transfer from certain toxigenic/ antibiotics-resistant to non-toxigenic/antibiotics-sensitive strains, respectively, should be worthy of attention.

• 수산경영론집
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• 제52권1호
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• pp.83-99
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• 2021

Fisheries policies are diversifying, including welfare programs for fishermen, revitalization of earfish and villages, and support for the sixth industrialization of fishing villages. In response to these policy changes, the purpose of this study is to categorize the fishing village fraternity, which is a local community, a fishery production organization, and a basic unit of a fishing village, and compares the characteristics of each type A number of indicators were selected by collecting data on the Categorization and evaluation of fishing villages fraternity and the statistical geographic information service. A number of indicators were extracted as representative factor variables using the principal component analysis, and then cluster analysis was performed to categorize the fishing village fraternity. This study was the first to attempt a comprehensive approach to revitalize the fishing village economy by using not only demographic and social characteristics, industrial and economic characteristics, but also regional characteristics. The characteristics of each type of fishing village fraternity find its significance in that it provides basic information that can be used in policy decisions. In order to strengthen the sustainability of the fishing community in the future and contribute to the sustainable development at the national level, the development of sustainable fishing village fraternity development indicators and follow-up studies on fishing village regeneration strategies will be needed.

• 한국농공학회논문집
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• 제64권3호
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• pp.1-8
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• 2022

In this study, a farm map utilization strategy for sustainable agricultural environmental resource management was derived. In addition, it is intended to present an efficient method of providing farm map-related services. As a result of the demand survey, the additional information required for the farm map includes 29% of information on crops grown on farmland, 21% of management-related information such as the owner or business entity, 17% of topographical information including slope, 15% of agricultural water information, 17% of land status information, and the addition of functions. 2% was investigated. As a result of intensive interview survey, it was found that it can be used for information on crops cultivated by agricultural businesses, actual cultivated area by township, arable land consolidation division boundary, and management of agricultural promotion zones. The farm map can be used as basic data to efficiently manage agricultural environmental resources. Since the status of support for individual farms or lots, such as soil improvement agent support and organic fertilizer support, may belong to personal information, it can be processed and provided in units required by administration or policies, such as administrative boundaries, subwatersheds, and watersheds. It can serve as a basis for executing the direct payment currently supported only by individual farms, even in a community unit that manages environmental direct payments.

• 농촌계획
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• 제27권4호
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• pp.109-122
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• 2021

The purpose of this study is to develop a application procedure and survey method for the empirical diagnostic indices system. In order to know the survey method, which has 65 investigation items for 52 indicators, this study not only presented so that the each survey items could be surveyed in at least 1 to 3 steps, but also presented the 4 survey method for each investigation items: preliminary survey, administrative survey, field survey, and interview survey. As a result of applying to 15 sample villages, the period of surveying a village of 100 households was 1.4 days, and it was confirmed that the survey was generally efficient in a short time. As a result of applying the survey system to the sample village, the developed survey system showed the possibility of investigating even the details of rural villages. Therefore, it was judged that it could be used as a basic procedure for a rural village unit survey.

• 한국해양학회지:바다
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• 제21권4호
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• pp.134-143
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• 2016

전국 및 지역별 갯벌을 대상으로 갯벌생태계의 생태적 과정을 유지하고 생태계서비스를 제공하는데 기본 토대인 자연환경에너지(태양, 바람, 파도, 강수, 조석 등)를 통한 에머지 유입 특성을 분석하였다. 우리나라 갯벌생태계로 유입하는 자연환경에너지가 공급한 에머지 총량은 $4.98{\times}10^{21}sej/yr$이었는데, 이는 갯벌생태계의 유지에 필요한 기본적인 환경 조건이 제공하는 에머지량에 해당한다. 갯벌생태계로 유입하는 에머지량을 화폐 단위로 환산하면 1조1,412억 원/yr으로, 이것은 갯벌생태계가 제공하는 생태계서비스를 생산하는데 토대가 되는 환경적 조건의 가치에 해당한다. 지역별 갯벌의 면적 차이를 고려하여 단위면적당으로 나타낼 경우 우리나라 연안의 일반적인 조차 분포를 따라 인천-경기 지역에서 부산 지역으로 갈수록 단위면적당 에머지 유입량이 감소하였다. 지역별 갯벌의 단위면적당 에머지 유입량과 단위면적당 수산물 생산의 에머지량을 이용하여 갯벌생태계를 유지하는데 필요한 자연환경에너지의 에머지 유입 특성과 생태계서비스 사이의 관계를 개괄적으로 살펴본 결과 지역별 갯벌로 유입한 에머지량과 수산물 생산의 에머지량 사이에는 아주 명확한 관계가 나타나지는 않았다. 그러나 단위면적당 에머지 유입량이 더 많은 서해안 갯벌에서 생산한 수산물의 에머지량이 남해안 갯벌에서 생산한 수산물의 에머지량보다 더 많아 개별 갯벌생태계의 생태계서비스 잠재력을 나타내는데 자연환경에너지를 통한 에머지 유입량의 활용 가능성을 보여주었다.

• 지적과 국토정보
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• 제46권2호
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• pp.283-296
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• 2016

본 연구의 목적은 최근 증가하고 있는 우리나라 아파트의 거래원인 가운데 하나인 분양권 전매비율을 중심으로 지역 간 특성을 파악하는데 있다. 이를 위해 162개 시 군 지역을 중심으로 인구특성, 아파트거래 및 분양특성, 주거특성, 경제적 특성을 살펴보았으며 분양권전매비율을 종속변수로 하는 다중회귀분석을 실시하였다. 그 결과 통계적으로 유의한 변수는 아파트 비율, 인구 증가율, 분양물량 평균 아파트 매매가격 대비 전세가격 비율, 1인 가구 증가율, 청약 경쟁률, 지역 내 매입자 거래호수 등 7개의 변수가 분양권 전매비율에 영향을 미치는 것으로 나타났다. 각 지역특성으로는 주거특성, 인구특성, 아파트거래 및 분양특성 순으로 영향을 미치는 것으로 나타났다. 본 결과로 아파트 시장에서 실수요자 중심의 거래를 활성화 하기위한 정부의 정책과 제도의 개선에 바탕이 되는 기초자료가 될 것으로 판단된다.

• 농촌계획
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• 제22권3호
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• pp.33-47
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• 2016

The purpose of this study is to understand the master plan of "The project on a comprehensive village development with a unit of a based township" through the master plan analysis and to help understand the implications of this project in order to maintain rural villages that can make local residents satisfied with them and promote the project of comprehensive rural village development as a successful way based on a comparative analysis through the in-site survey. The processes of this study are as follows. First, the analysis of development planning created a drawing based on linking business strategy by each region. The current situation and characteristics of planned public spaces were analyzed through the analysis of location and distribution of public spaces and facilities. Second, the situation analysis was performed by the task of shooting based on the basic plan information, and the master plan and status were confirmed through interviews. Also, the analysis of location and distribution of public spaces and facilities was conducted by a created drawings based on a survey. Finally, the detailed information of public spaces and facilities was compared with the investigation by in-site survey and analyzed through the Master Plan Strategy and a detailed classification of the types of facilities among each regional issues. In addition, by overlapping the planned drawings with the results, the results of formations were confirmed and the planned positional changes and the distribution of facilities were analyzed. As the results of "The project of comprehensive village development with a unit of based township", it was determined that there were many issues on cultural and welfare facilities in this project to meet the business objectives. Also, the facilities the residents needed, identified by interviews, were rural tourism facilities. According to the results, the residents cherished rural tourism facilities as much as basic living facilities. However, as the results of comparative analysis of public spaces and facilities, it was confirmed that mostly the plan was unfulfilled. Therefore, it is concluded that living, cultural and welfare facilities in the township can be maintained and expanded comprehensively as a purpose of "The project of comprehensive village development with a unit of based township" after not only planning process but also the project is fulfilled actually in rural areas.

• 한국식품저장유통학회지
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• 제16권2호
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• pp.217-222
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• 2009

재래식 메주의 산업화의 공정관리를 위한 기초자료를 제시하기 위해 강원도 원주를 비롯한 전국 17개 지역에서 구입한 재래식 메주의 수분 등의 이화학적 성분, $\alpha$-amylase 등의 효소분석 및 생균수 등의 미생물 분석을 하였다. 전국에서 구입한 메주의 형태는 사각형의 벽돌 모양이 8개 지역, 9개지역은 둥근 모양을 띄고 있었다. 재래식 메주의 수분은 강원도 정선 메주가 최저 9.83%(w/w)인 반면 전북 정읍메주가 약 3.7배 많은 36.24%(w/w)를 나타내고 있었다. 조지방의 경우 무수물 기준으로 충북 청주에서 구입한 재래식 메주가 17.46%(w/w)로 가장 낮은 반면 전북 정읍에서 구입한 메주가 약 1.6배 많은 28.74%(w/w)를 나타내고 있었다. 조단백질의 경우 경기도 여주에서 구입한 메주가 무수물 기준으로 최저 42.00%(w/w)를 보인 반면 강원도 원주에서 구입한 메주가 45.54%(w/w)를 보이고 있었다. 재래식 메주의 숙성도를 나타내는 아미노태질소는 충북 제천시에서 구입한 메주가 무수물 기준으로 223.65 mg%인 반면 전북 김제의 메주가 약 5.6배 많은 1137.68 mg%를 나타내고 있었다. 재래식 메주의 색도를 측정한 결과 전북 김제에서 구입한 메주가 가장 어두운 색을 띄고 있어 기호도 측면에서 낮은 면을 보이고 있었다. 재래식 메주의 $\alpha$-amylase는 무수물 기준으로 $130.32{\sim}1254.45$ unit로 지역간에 약 9.6배의 차이를 보이고 있었으며 $\beta$-amylase는 무수물 기준으로 $30.07{\sim}167.88$ unit로 지역에 따라 약 5.6배의 차이가 나고 있었다. 산성 protease는 무수물 기준으로 $72.53{\sim}340.04$ unit로 약 4.6배의 차이를 보이고 있어 각 효소의 역가는 지역에 따라 차이가 매우 큰 것으로 나타났다. 재래식 메주의 생균수는 $4.8{\times}10^7{\sim}2.6{\times}10^{10}cfu/g$, 곰팡이 및 효모수는 $4.3{\times}10^4{\sim}7.9{\times}10^6cfu/g$으로 지역에 따라 메주의 균수가 다양하게 나타났다.