• Journal of Microbiology and Biotechnology
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• v.25 no.8
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• pp.1380-1389
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• 2015

Prx1, an AhpF-dependent 2-Cys peroxiredoxin (Prx), was previously identified in Vibrio vulnificus, a facultative aerobic pathogen. In the present study, transcription of the V. vulnificus prx1ahpF genes, which are adjacently located on the chromosome, was evaluated by analyzing the promoter and intergenic region of the two genes. Northern blot analyses revealed that transcription of prx1ahpF results in two transcripts, the prx1 and prx1ahpF transcripts. Primer extension analysis and a point mutational analysis of the promoter region showed that the two transcripts are generated from a single promoter. In addition, the 3' end of the prx1 transcript at the prx1ahpF intergenic region was determined by a 3'RACE assay. These results suggested that the prx1ahpF genes are transcribed as an operon, and the prx1 transcript was produced by transcriptional termination in the intergenic region. RNA secondary structure prediction of the prx1ahpF intergenic region singled out a stem-loop structure without poly(U) tract, and a deletion analysis of the intergenic region showed that the atypical stem-loop structure acts as the transcriptional attenuator to result in the prx1 and prx1ahpF transcripts. The combined results demonstrate that the differential expression of prx1 and ahpF is accomplished by the cis-acting transcriptional attenuator located between the two genes and thereby leads to the production of a high level of Prx1 and a low level of AhpF.

• Journal of Microbiology and Biotechnology
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• v.22 no.9
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• pp.1177-1184
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• 2012

In order to estimate how diverse the mating types in Pleurotus eryngii from different regions are, pairings between monokaryons derived from inter- and intra-groups were done. Sixteen and 15 alleles were identified at loci A and B from the 12 strains. In the P. eryngii KNR2312, widely used for commercial production, four mating loci, A3, A4, B3, and B4, were determined. Those loci, except A3, were found in 4 strains out of 12 strains. To improve breeding efficiency, especially in mating type determination, RAPD and BSA were performed to screen for a mating type specific marker. The SCAR marker 13-$2_{2100}$ was developed based on the RAPD-derived sequence typing B3 locus. The sequence analysis of 13-$2_{2100}$ revealed that it contained a conserved domain, the STE3 super-family, and consensus sequences like the TATA box and GC box. It seems likely that the SCAR marker region is a part of the pheromone receptor gene.

• The Journal of Advanced Prosthodontics
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• v.16 no.3
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• pp.139-150
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• 2024

PURPOSE. The purpose of this diagnostic study was to assess the accuracy and time efficiency of a digital method to draw the denture foundation extension outline on preliminary casts compared with the conventional technique. MATERIALS AND METHODS. A total of 28 preliminary edentulous casts with no anatomical landmarks were digitized using a laboratory scanner. The outlining of the entire basal seat of the denture was performed on preliminary casts and digitized. Casts with no extension outline were digitized and outlines were drawn using software (DWOS, Straumann). The accuracy of the extension outlined between both techniques was evaluated in the software (GOM Inspect; GOM GmbH) by file superimposition. Specificity and sensitivity tests were applied to measure accuracy. The paired t-test (95% CI) was used to compare the mean total area and the working time. RESULTS. The accuracy ranged from 0.57 to 0.92. The buccal and labial frenulum showed a lower value in the maxilla (0.57); while the area between the retromolar pad and buccal frenulum (0.64) showed a lower score in the mandible. The maxillary denture foundation and the working time for both arches were significantly longer for the digital method (P < .001). CONCLUSION. The denture foundation extension outline exhibited a sufficiently excellent accuracy for the digital method, except for the maxillary anterior region. However, the digital method required a longer working time.

• Research in Plant Disease
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• v.26 no.1
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• pp.19-28
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• 2020

Jujube witches' broom (JWB) disease is a high-risk plant disease that occurred on and devastated jujube orchards throughout South Korea in the 1950s. Recently, as the jujube cultivation area has been significantly increased nationwide, we investigated the disease in jujube orchards in the Boeun region to obtain basic information for proactively evaluating the risk of JWB outbreaks from 2017 to 2019. A field trial was conducted to evaluate the effect of the trunk injection of Sungbocycline (a. i. oxytetracycline calcium alkyl trimethyl ammonium 17%, OTC). The incidence of JWB disease was very low in Boeun, but it was gradually increased mainly in the area and on the farms where it had occurred previously. The trunk injection with OTC was most effective in the early stages of growth. However, even though the trees were treated with OTC, disease symptoms appeared in other healthy branches in severely affected trees. When the tree was first injected with OTC in June, the control efficacy of second injection was higher one month after the first injection than five months. These results suggest that early diagnosis based on various symptoms and early control based on them can be very effective in preventing JWB disease in advance and increasing control efficacy.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.56 no.2
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• pp.99-106
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• 2011

Here we reported an analyzing result for the relationship between climatic variables and rice(c.v. Odaebyeo and Ilpumbyeo) yield characteristics (including some growth characteristics) based on a long-term observed data at GARES and at KMA for rice and weather, respectively. Most of crop parameters investigated, such as heading date, culm height, panicle number $m^{-2}$, grain number $panicle^{-1}$ ripening rate, 1,000 grain weight and yield were strongly affected by wind velocity and relative humidity, as well as by daily mean air temperature, precipitation, sunshine hours and daily variations in air temperature depending on variety and crop developmental stages. Air velocity and relative humidity had not been studied as climatic variables affecting on the characteristics of rice growth and yield, however, they turned out to affect all the characteristics of rice investigated, especially ripening rate and yield, as much as any other climatic variables in this study. Air velocity appeared to affect highly the culm height and yield of Odaebyeo and ilpumbyeo. Relative humidity appeared to affect highly grain number and ripening rate of Odaebyeo and yield of Ilpumbyeo. Consequently Rice yield revealed to increase in the climatic conditions of high air velocity and low relative humidity.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.62 no.2
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• pp.79-86
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• 2017

This study was conducted to analyze seasonal and annual variations in rice quality and factors affecting the quality, for quality evaluation of the brand rice varieties produced in Jeonnam region. Coefficient of variation (CV) values for the seasonal variation in the rice quality were 3.1% in Toyo value, 2.1% in whiteness, 1.6% in protein content, 1.0% in moisture content, and 0.4% in head rice ratio. Quality characteristics of the brand rice varieties generally showed a decreasing tendency after April, as the months progressed. CV values for the annual variation in the rice quality were relatively high at 5.6% in protein content and 5.2% in Toyo value whereas those for whiteness and head rice ratio were relatively low, at 2.7% and 1.8%, respectively. Palatability and protein content showed high correlations with minimum air temperature, sunshine hours, rainfall, and daily temperature range. Head rice ratio had a negative correlation with daily temperature range whereas chalky rice ratio had a positive correlation with rainfall. Based on these results, we formulated a multiple regression equation to estimate palatability of cooked rice using protein content, whiteness, head rice ratio, and moisture content as follows: y = - 6.71a + 2.27b + 1.29c + 0.51d - 15.34 ($R^2$=0.51*) (y: palatability of cooked rice, a: protein content, b: moisture content, c: whiteness, d: head rice ratio).

• Proceedings of the KIPE Conference
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• 2003.07a
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• pp.477-480
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• 2003

Generally, 2 phase excited brushless DC motor(BLDCM) operates only in the region of constant trapezoidal back-EMF on each phase. Therefore the efficiency decreases in comparison with 3phase excited BLDCM. This paper proposes the relation between the usage ratio of voltage and the change of fewer delivery of BLDCM according to extension of conduction intervals.

• Communications for Statistical Applications and Methods
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• v.5 no.1
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• pp.107-120
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• 1998

This research aims here to develop a certain extended double sampling plan, EDS, which is an extension of ordinary double sampling plan in the sense that the second-stage sampling effort and second-stage critical value are allowed to depend on the point at which the first-stage continuation region is traversed. For purpose of comparison, single sampling plan, optimal ordinary double sampling plan(ODS) and sequential probability ratio test are considered with the same overall error rates, respectively. It is observed that the EDS idea allows less sampling effort than the optimal ODS.

• Korean Journal of Plant Resources
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• v.37 no.4
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• pp.392-400
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• 2024

This study was conducted to find the flowering and growth characteristics according to the different altitudes (plains and mid-mountain regions) and cultivation methods (field and plastic houses cultivation) of Elsholtzia splendens. Experimental regions located at 12 meters and 500 meters above sea level were selected for the plains and the mid-mountain, respectively, and the same method was applied for cultivation management by different altitudes and cultivation methods. In the mid-mountain region, flower bud emergence (2-3 days), flowering (9 days), and full bloom (6-7 days) stages of Elsholtzia splendens were earlier than in the plains, and field cultivation was earlier than plastic house cultivation. The plant height, the main stem diameter, and the number of branches tended to increase gradually after an initial rapid growth at 59 to 69 days after planting date. The days of duration of sunshine (less than 8 hours) from the rainy season (June 20) to the period when vegetative growth increases gradually (59 to 69 days after planting) was 22 to 29 days and 26 to 35 days in the plains and the mid-mountain regions respectively, and this period was estimated time of transition from vegetative growth to reproductive growth. The spikes growth of Elsholtzia splendens by cultivation altitudes was higher in the mid-mountain region than in the plains, and there were no statistically significant differences in growth characteristics except for the main stem diameter, the number of branches, and the dry matter. Also, the amount of flowering and growth was higher in the plastic house cultivation compared to the field cultivation. As a result, some differences in flowering amount were observed when cultivating Elsholtzia splendens for landscaping purposes, but it was considered possible to cultivate in both plains and mid-mountain regions. This study therefore provides ecological information for understanding the relationship between weather characteristics and growth of Elsholtzia splendens.

• Proceedings of the KSRS Conference
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• v.2
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• pp.880-883
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• 2006

Altimeter(Topex/Poseidon) and AVHRR(NOAA) data were used to study the variations and correlations of Sea Level(SL) and Sea Surface Temperature (SST) in the North East Asian Seas from November 1993 to May 1998. This region is influenced simultaneously to continental and oceanic climate as the border of the East Sea(Japan Sea). SL and SST have increased gradually every year because the global warming, and presented usually a strong annual variations in Kuroshio extension region with the influence of bottom topography.