• The Transactions of The Korean Institute of Electrical Engineers
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• v.68 no.1
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• pp.44-51
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• 2019

Tunnel Boring Machine's Technology has depends mostly on imports, currently domestic technology development was proceeding. There are many technologies in this field, above all, the large-capacity motor drive technology required for excavation is one of the core technologies. In particular, when several large motors are simultaneously starting, there are many problems due to a large starting current at that time, and it is difficult to design and operate a power receiving facility. In this paper, A method of reducing the starting current by using the regenerative power generated by the deceleration of the motor has been studied. To verify this proposal, we designed the induction motor controller using CAE based power simulation tool and verified the results of the proposed method by applying the reduced model. As a result, it is possible to reduce the maximum starting current and shorten the start-up time. Moreover, even if several motors are connected to one bank, it is proved that the method can be efficiently operated by using the sequential braking / starting sequence. In the case of a power system in which a large capacity electric motor such as a tunnel excavation system is driven, the results of this study are expected to be a stable and effective method for solving the start-up current problem and designing the power receiving facility.

• Biomolecules & Therapeutics
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• v.24 no.3
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• pp.260-267
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• 2016

Mesenchymal stem cells (MSCs) offer significant therapeutic promise for various regenerative therapies. However, MSC-based therapy for injury exhibits low efficacy due to the pathological environment in target tissues and the differences between in vitro and in vivo conditions. To address this issue, we developed adipose-derived MSC spheroids as a novel delivery method to preserve the stem cell microenvironment. MSC spheroids were generated by suspension culture for 3 days, and their sizes increased in a time-dependent manner. After re-attachment of MSC spheroids to the plastic dish, their adhesion capacity and morphology were not altered. MSC spheroids showed enhanced production of hypoxia-induced angiogenic cytokines such as vascular endothelial growth factor (VEGF), stromal cell derived factor (SDF), and hepatocyte growth factor (HGF). In addition, spheroid culture promoted the preservation of extracellular matrix (ECM) components, such as laminin and fibronectin, in a culture time- and spheroid size-dependent manner. Furthermore, phosphorylation of AKT, a cell survival signal, was significantly higher and the expression of pro-apoptotic molecules, poly (ADP ribose) polymerase-1 (PARP-1) and cleaved caspase-3, was markedly lower in the spheroids than in MSCs in monolayers. In the murine hindlimb ischemia model, transplanted MSC spheroids showed better proliferation than MSCs in monolayer. These findings suggest that MSC spheroids promote MSC bioactivities via secretion of angiogenic cytokines, preservation of ECM components, and regulation of apoptotic signals. Therefore, MSC spheroid-based cell therapy may serve as a simple and effective strategy for regenerative medicine.

• The Journal of the Korea institute of electronic communication sciences
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• v.11 no.2
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• pp.179-184
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• 2016

The electrical load testing system developed from this study was designed to control rated-capacity-testing or variable-load-testing in an active and precise manner and save electric energy during testing, and also to convert the saved electric energy through the electrical load testing system to grid line. As for the device under testing, it was designed to be applied to not only transformer, rectifier, voltage regulator, inverter which require grid voltage source but, also applied to electric power, aerogenerator, photovoltaic, hybrid generator, battery, etc. which do not require grid voltage source. The system was designed to return the power consumed during the testing to the grid line by connecting the synchronizing pwm inverter circuit to the grid voltage source, and was also made to enable the being-tested system from disuse of approximately 93.4% energy when compared to the conventional load testing system which has used the passive resistor.

• BMB Reports
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• v.52 no.12
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• pp.689-694
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• 2019

Ethical and safety issues have rendered mesenchymal stem cells (MSCs) popular candidates in regenerative medicine, but their therapeutic capacity is lower than that of induced pluripotent stem cells (iPSCs). This study compared original, dental tissue-derived MSCs with re-differentiated MSCs from iPSCs (iPS-MSCs). CD marker expression in iPS-MSCs was similar to original MSCs. iPS-MSCs expressed higher in pluripotent genes, but lower levels in mesodermal genes than MSCs. In addition, iPS-MSCs did not form teratomas. All iPSCs carried mtDNA mutations; some shared with original MSCs and others not previously detected therein. Shared mutations were synonymous, while novel mutations were non-synonymous or located on RNA-encoding genes. iPS-MSCs also harbored mtDNA mutations transmitted from iPSCs. Selected iPS-MSCs displayed lower mitochondrial respiration than original MSCs. In conclusion, screening for mtDNA mutations in iPSC lines for iPS-MSCs can identify mutation-free cell lines for therapeutic applications.

• Tissue Engineering and Regenerative Medicine
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• v.15 no.6
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• pp.673-697
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• 2018

BACKGROUND: Cartilage tissue engineering (CTE) aims to obtain a structure mimicking native cartilage tissue through the combination of relevant cells, three-dimensional scaffolds, and extraneous signals. Implantation of 'matured' constructs is thus expected to provide solution for treating large injury of articular cartilage. Type I collagen is widely used as scaffolds for CTE products undergoing clinical trial, owing to its ubiquitous biocompatibility and vast clinical approval. However, the long-term performance of pure type I collagen scaffolds would suffer from its limited chondrogenic capacity and inferior mechanical properties. This paper aims to provide insights necessary for advancing type I collagen scaffolds in the CTE applications. METHODS: Initially, the interactions of type I/II collagen with CTE-relevant cells [i.e., articular chondrocytes (ACs) and mesenchymal stem cells (MSCs)] are discussed. Next, the physical features and chemical composition of the scaffolds crucial to support chondrogenic activities of AC and MSC are highlighted. Attempts to optimize the collagen scaffolds by blending with natural/synthetic polymers are described. Hybrid strategy in which collagen and structural polymers are combined in non-blending manner is detailed. RESULTS: Type I collagen is sufficient to support cellular activities of ACs and MSCs; however it shows limited chondrogenic performance than type II collagen. Nonetheless, type I collagen is the clinically feasible option since type II collagen shows arthritogenic potency. Physical features of scaffolds such as internal structure, pore size, stiffness, etc. are shown to be crucial in influencing the differentiation fate and secreting extracellular matrixes from ACs and MSCs. Collagen can be blended with native or synthetic polymer to improve the mechanical and bioactivities of final composites. However, the versatility of blending strategy is limited due to denaturation of type I collagen at harsh processing condition. Hybrid strategy is successful in maximizing bioactivity of collagen scaffolds and mechanical robustness of structural polymer. CONCLUSION: Considering the previous improvements of physical and compositional properties of collagen scaffolds and recent manufacturing developments of structural polymer, it is concluded that hybrid strategy is a promising approach to advance further collagen-based scaffolds in CTE.

• Clinical and Experimental Pediatrics
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• v.53 no.8
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• pp.786-789
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• 2010

Induced pluripotent stem (iPS) cells are generated by epigenetic reprogramming of somatic cells through the exogenous expression of transcription factors. Recently, the generation of iPS cells from patients with a variety of genetic diseases was found to likely have a major impact on regenerative medicine, because these cells self-renew indefinitely in culture while retaining the capacity to differentiate into any cell type in the body, thereby enabling disease investigation and drug development. This review focuses on the current state of iPS cell technology and discusses the potential applications of these cells for disease modeling; drug discovery; and eventually, cell replacement therapy.

• 대한치주과학회:학술대회논문집
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• 2007.11a
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• pp.105-106
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• 2007

• 대한치주과학회:학술대회논문집
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• 2001.12a
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• pp.81-82
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• 2001

• Proceedings of the Korean Society of Machine Tool Engineers Conference
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• 2001.04a
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• pp.15-21
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• 2001

An accumulator in hydraulic systems stores kinetic energy during braking action, and then that controls hasty surge pressure. An energy recovery system using accumulator seems to be advantageous for ERBS due to its high energy density. This study suggests a method to decide suitable accumulator volume for ERBS. The method is based upon energy conservation between kinetic energy of moving inertia and elastic energy of accumulator. The energy conversion was analyzed and a simple formula was derived. A series of computer simulation was done to verify effectiveness of the formula. The results of the simulation work were compared with those of experiments and these results show that the proposed design is effective for decision accumulator volume in ERBS.

• The Journal of the Korean dental association
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• v.44 no.6 s.445
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• pp.359-366
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• 2006