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Korean Society for Biochemistry and Molecular Biology (생화학분자생물학회)
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Mitochondrial genome mutations in mesenchymal stem cells derived from human dental induced pluripotent stem cells

  • Park, Jumi (Department of Convergence Medicine & Stem Cell Center, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Lee, Yeonmi (Department of Convergence Medicine & Stem Cell Center, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Shin, Joosung (Department of Convergence Medicine & Stem Cell Center, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Lee, Hyeon-Jeong (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Son, Young-Bum (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Park, Bong-Wook (Department of Dentistry, Gyeongsang National University School of Medicine, Institute of Health Science) ;
  • Kim, Deokhoon (Department of Pathology, Asan Institute for Life Sciences, Asan Medical Center, University of Ulsan College of Medicine) ;
  • Rho, Gyu-Jin (Department of Theriogenology and Biotechnology, College of Veterinary Medicine, Gyeongsang National University) ;
  • Kang, Eunju (Department of Convergence Medicine & Stem Cell Center, Asan Medical Center, University of Ulsan College of Medicine)
  • Received : 2019.02.13
  • Accepted : 2019.06.12
  • Published : 2019.12.31
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Abstract

Ethical and safety issues have rendered mesenchymal stem cells (MSCs) popular candidates in regenerative medicine, but their therapeutic capacity is lower than that of induced pluripotent stem cells (iPSCs). This study compared original, dental tissue-derived MSCs with re-differentiated MSCs from iPSCs (iPS-MSCs). CD marker expression in iPS-MSCs was similar to original MSCs. iPS-MSCs expressed higher in pluripotent genes, but lower levels in mesodermal genes than MSCs. In addition, iPS-MSCs did not form teratomas. All iPSCs carried mtDNA mutations; some shared with original MSCs and others not previously detected therein. Shared mutations were synonymous, while novel mutations were non-synonymous or located on RNA-encoding genes. iPS-MSCs also harbored mtDNA mutations transmitted from iPSCs. Selected iPS-MSCs displayed lower mitochondrial respiration than original MSCs. In conclusion, screening for mtDNA mutations in iPSC lines for iPS-MSCs can identify mutation-free cell lines for therapeutic applications.

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References

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