• Journal of Periodontal and Implant Science
• /
• 제31권1호
• /
• pp.123-135
• /
• 2001

Several growth factors and polypeptides were studied for the regeneration of periodontal supporting tissues which had been lost due to periodontal disease. But these are not commonly used for regenerators of bone tissue or alveolar bone, because of the insufficiency of studies on their side effects, genetic engineering for mass production and stability for clinical application. Recently, many natural products, which have advantage of less side effects and possibility of long-term use, have been studied for their capacity and effects of anti-bacterial, anti-inflammatory and regenerative potential or periodontal tissues. Cnidii Rhizoma, Rhinocerotis Cornu and Drynariae Rhizoma have been traditionally used as a drug for treatment of bone disease in oriental medicine. The purpose of this study was to examine the ability of alkaline phosphatase synthesis of MC3T3-E1 cells when above medicines were supplimented. MC3T3-E1 cells were cultured with ${\alpha}-MEM(negative control)$, dexamethasone(positive control), and each natural products for 3 and 5 days. And then ALP synthesis was measured by spectrophotometer for enzyme activity and by naphthol AS-BI staining for morphometry. Except Cnidii Rhizoma, all of the natural products of this study induced higher activity of ALP synthesis than controls. Among them Drynariae Rhizoma induced the highest activity. In the aspects of culturing time, all medicines did not showed the difference between 3 and 5 days, but $10^{-7}g/ml$ group of Rhinocerotis Corun showed significant increase at 3 days than at 5 days. These results indicate that several natural products have a inducing ability of ALP synthesis on osteoblasts.

• Maxillofacial Plastic and Reconstructive Surgery
• /
• 제31권6호
• /
• pp.544-549
• /
• 2009

Failure to use effective methods of reduction, fixation and immobilization may lead to osteomyelitis with the exposed necrotic bone, as the overzealous use of transosseous wires & plates that devascularizes bone segments in the compound comminuted fractures of mandible. Once osteomyelitis secondary to fractures has become established, intermaxillary fixation should be instituted as early as possible. Fixation enhances patient comfort and hinders ingress of microorganisms and debris by movement of bone fragments. Teeth and foreign materials that are in the line of fracture should be removed and initial debridement performed at the earliest possible time. Grossly necrotic bone should be excised as early as possible ; no attempt should be made to create soft tissue flaps to achieve closure over exposed bone. The key to treatment of chronic osteomyelitis of the mandible is adequate and prolonged soft tissue drainage. If good soft tissue drainage is provided over a long period, sequestration of infected bone followed by regeneration or fibrous tissue replacement will occur so that appearance and function are not seriously altered. Localization and sequestration of infected mandible are far better performed by natural mechanism of homeostasis than by cutting across involved bone with a cosmetic or functional defect. As natural host defenses and conservative therapy begin to be effective, the process may become chronic, inflammation regresses, granulation tissue is formed, and new blood vessels cause lysis of bone, thus separating fragments of necrotic bone(sequestra) from viable bone. The sequestra may be isolated by a bed of granulation tissue, encased in a sheath of new bone(involucrum), and removed easily with pincettes. This is a case report of the long-term conservative drainage care in osteomyelitis associated with mandibular fractures.

• 구강회복응용과학지
• /
• 제25권3호
• /
• pp.267-277
• /
• 2009

구강 내 단일 치아를 상실한 경우 기존에는 고정성 보철물을 이용하여 수복하는 방법이 일반적이었지만, 최근에 와서는 임플란트를 이용하여 수복하는 것이 보편화되고 있다. 본 연구는 단일 치아 결손 시 $TiUnite^{TM}$ 표면 처리한 임플란트를 이용하여 수복한 경우를 후향적으로 조사하여 그 생존율을 분석한 것이다. 2002년 9월부터 2006년 12월까지 삼성서울병원에서 단일 치아 결손 부위에 식립된 총 269개의 $TiUnite^{TM}$ 표면 처리한 임플란트 중 21개는 인접한 임플란트가 있거나, 기록이 누락되거나 관찰 기간이 짧아 연구에서 제외되었다. 248개의 임플란트 중 상악에는 129개(52.0%) 하악에는 119개(48.0%) 식립되었다. 수술 부위에 치조골 재생술을 시행한 경우는 총 100개(40.3%)였으며, 상악동 거상술이 시행된 증례는 총 36개(14.5%)였다. 수술 당일부터 관찰 기간은 평균 $26.0{\pm}11.8$ 개월이었으며, 그 기간 동안 실패한 것으로 간주된 임플란트는 12개로 생존율은 95.2%였다. 그 중 상악에서 실패한 경우가 10개, 하악에서는 2개로 각각의 생존율은 92.2%, 98.3% 이다. 단일 치아 결손 시 $TiUnite^{TM}$ 표면 처리한 임플란트를 이용하여 수복한 경우 단기간 동안 높은 생존율을 보였다.

• Journal of Periodontal and Implant Science
• /
• 제26권4호
• /
• pp.841-858
• /
• 1996

Epidermal growth factor(EGF) is one of polypeptide growth factors. EGF has been reported as a biological mediator which regulates activities of wound healing process including the cell proliferation, migration and metabolism. The purposes of this study is to evaluate the effects of EGF on the human periodontal ligament cells and human gingival fibroblast cells that promote regeneration of periodntal tissue. The mitogenic effects of epidermal growth factor on human periodontal ligament cells and human gingival fibroblasts were evaluated by determining the incorporation of 5-Bromo-2'-deoxy-uridine into DNA of the cells in a dose dependent manner. The prepared cells were the primary cultured gingival fibroblast and periodontal ligament cells from humans, the fourth or sixth subpassages were used in the experiments. Cells were seeded in DMEM containing 10% FBS. 1, 10, 50, 100, $200{\eta}g/ml$ and epidermal growth factor were added to the quiescent cells for 24 hours, 48 hours and 72 hours. They were labeled with $10\{mu}l/200{\mu}l$ 5-Bromo-2'-deoxy-uridine for the last 6 hours of each culture. The results of the five determinants were presented as mean and S.D.. The results were as follows : The DNA synthetic activity of human gingival fibroblasts were increased dose dependently by epidermal growth factor at 24 hours, 48 hours and 72 hours. The mitogenic effects were similar at the 24 and 48 hours of epidermal growth factor, but the DNA synthetic activity of human gingival fibroblasts generally decreased at 72 hours. The DNA synthetic activity of human periodontal ligament cells were increased dose dependently by epidermal growth factor at 24 hours but the DNA synthetic activity decreased at $200{\eta}g/ml$ of each hour. Generally the maximum mitogenic effects were observed at the 48 hours application of epidermal growth factor. The DNA synthetic activity of human periodontal ligament cells generally decreased lower at 24, 72 hours than at 48 hours the application of epidermal growth factor. In the comparison of DNA synthetic activity between human gingival fibroblasts and human periodontal ligament cells, human periodontal ligament cells had slightly higher proliferation activity than human gingival fibroblasts for a longer time at the high dosage of the epidermal growth factor. In conclusion, epidermal growth factor have important roles in the stimulation of DNA synthesis in human periodontal ligament cells and human gingival fibroblasts, and thus may be useful for clinical applications in periodontal regenerative procedures.

• Journal of Periodontal and Implant Science
• /
• 제37권1호
• /
• pp.35-44
• /
• 2007

Periodontal ligament (PDL) is the connective tissue located between the tooth root and alveolar bone. In a previous study, PDLs22 was isolated as a PDL-specific gene by using subtractive hybrid-ization between cultured PDL fibroblasts and gingival fibroblasts. It was also suggested that PDLs22 plays important roles in the development, differentiation and maintenance of periodontal tissues. However, little is known about functional study of PDLs22 using recombinant protein in PDL fibroblast differentiation and periodontium formation. In this study, in order to produce the PDLs22 recombinat protein, PDLs22 expression vector were constructed and expressed its protein in various host cell and temperature conditions. The results were as follows: 1. PDLs22 protein was not strongly expressed In the induction system using pRSET-PDLs22 construct. 2. When the BL21(DE3) pLysS was used as a expression host, PDLS22 protein was strongly ex-pressed in the induction system using pHCEIIBNd-PDLs22 construct. 3. The PDLs22 protein was recognized at a molecular weight of 28 kDa in western blots. 4. Almost of the expressed PDLs22 protein was not soluble and observed like as inclusion body. 5. The protein solubility was not improved after modification of induction time and temperature during PDLs22 protein production. In this study, the system for the PDLs22 protein production was connstructed. However, the re-results suggest that further studies will be needed to produce the considerable amount of PDLs22 re-combinat protein, which can use for the periodontal regeneration.

• Archives of Plastic Surgery
• /
• 제40권4호
• /
• pp.359-366
• /
• 2013

Background Pedicled transverse rectus abdominis musculocutaneous flaps typically sacrifice the entire muscle. In our experience, the lateral strip of the rectus abdominis muscle can be spared in an attempt to maintain function and reduce morbidity. When the intercostal nerves are injured, muscle atrophy appears with time. The severed intercostal nerve was reinserted into the remnant lateral strip of the rectus abdominis muscle to reduce muscle atrophy. Methods The authors retrospectively reviewed 9 neurotized cases and 10 non-neurotized cases. Abdominal computed tomography was performed to determine the area of the rectus muscles. Electromyography (EMG) was performed to check contractile function of the remnant muscle. A single investigator measured the mean areas of randomly selected locations (second lumbar spine) using ImageJ software in a series of 10 cross-sectional slices. We compared the Hounsfield unit (HU) pre- and postoperatively to evaluate regeneration quality. Results In the neurotization group, 7 of 9 cases maintained the mass of remnant muscle. However, in the non-neurotization group, 8 of 10 lost their mass. The number of totally atrophied muscles in each of the two groups was significantly different (P=0.027). All of the remnant muscles showed contractile function on EMG. The 9 remaining remnant rectus abdominis muscles showed declined the HU value after surgery but also within a normal range of muscle. Conclusions Neurotization was found to be effective in maintaining the mass of remnant muscle. Neurotized remnant muscle had contractile function on EMG and no fatty degeneration by HU value.

• 한국해양학회지
• /
• 제25권1호
• /
• pp.8-20
• /
• 1990

조간대 퇴적물의 공극수 화학과 초기 속성작용을 연구하기 위하여, 경기만 남동부 의 반월지역 조간대의 한 정점에서 1987년 10월 -1988년 10월 사이에 6차의 공극수 시 료가 채위 분석되었다. 시료채취 지점은 세립질 뻘 퇴적물이 분포하며 무산소 환경으 로 인해 생물교란이 없는 곳으로서, 미생물에 의한 유기물 분해와 영양염의 재생이 매 우 활발하였다. 공극수 내의 황상염 환원은 매우 급속히 이루어져, 해수로부터 공급된 황산염이 10 cm 깊이 이내에서 일차적으로 체거되는 현상이 겨울철을 제외한 전 시기 에 관찰되었다. 황산염 환원과 유기물 분해의 산물로서 생겨난 암모니아와 인산염은 공극수 내에 축적되어 수직적으로 심한 농도구배를 나타냈다. 봄철에서 초여름에 걸친 시기에는, 한편, 일차적인 황상염 환원대 아래쪽으로 2차적인 농도증가현상이 관찰되 었다. 공극수 내 황상염, 암모니아 및 인삼염 농도의 수직 분포에서는 계절적인 차이 가 나타났다. Berner (1980)의 속성모델을 적용하여 황산염 환원과 영양염류 재생의 반응속도를 정량화시킨 결과 여름철과 겨울철 사이에 약 10배의 차이를 나타냈으며, 이 차이는 계절적인 온도의 차이에 기인한 것으로 해석되었다. 형양염 농도구배로부터 계산된 C : N : P 비도 계절적인 차이를 나타냈다.

• 대한화장품학회지
• /
• 제44권3호
• /
• pp.277-284
• /
• 2018

콜라겐 펩타이드(collagen peptide)는 단백질의 가수 분해물로서 주름 완화, 보습력 증대, 탄력 개선 등의 특정 피부 효능을 나타낼 수 있어 화장품 혹은 피부 개선 기능식품으로서 활용되고 있다. ${\gamma}-aminobutyric$ acid (GABA)는 척추 동물의 뇌, 척수에 존재하는 신경전달 물질로서 수면의 질과 양을 개선해 준다고 알려져 있다. 본 연구에서는 콜라겐 펩타이드와 GABA의 복합물이 수면 장애가 있는 여성에게 8주 경구 섭취를 통해서 수면 및 피부 상태를 개선할 수 있는가에 관하여 확인하였다. 복합물(J85091900)은 8주간 연속적으로 섭취 시, 수면장애지수(PSQI)가 유의적으로 감소하였으며, 수면 시간을 7% 증가시켰다. 또한, 피부 거칠기, 눈가 주름 및 피부 수분량(capacitance)을 유의적으로 개선하였다. 이상의 결과에서 콜라겐과 GABA의 복합물은 복합 수면 장애에 따른 피부의 노화 현상으로부터 피부를 보호할 수 있음을 확인하여 먹는 화장품의 핵심 소재로 활용 가능함을 확인하였다.

• 대한수의학회지
• /
• 제46권4호
• /
• pp.305-313
• /
• 2006

The primary goal of the wound healing is rapid wound closure. Recent advances in cellular and molecular biology have greatly expanded our understanding of the biologic processes involved in wound repair and tissue regeneration. This study was conducted to develop a new sponge type of biomaterial to be used for either wound dressing or scaffold for tissue engineering. We designed to make a comparative study of the wound healing effect of silk fibroin/hyaluronic acid (SF/HA) blend sponge in full-thickness dermal injury model of rat. Two full-thickness excisions were made on the back of the experimental animals. The excised wound was covered with either the silk fibroin (SF), hyaluronic acid (HA) or SF/HA (7 : 3 or 5 : 5 ratio) blend sponge. On the postoperative days of 3, 7, 10 and 14, the wound area was calculated by image analysis software. Simultaneously, the tissues were stained with Hematoxylin-Eosin and Masson's trichrome methods to measure the area of regenerated epithelium and collagen deposition. In addition, we evaluated the degree of the epithelial cell proliferation using immunohistochemistry for proliferating cell nuclear antigen (PCNA). We found that the half healing time ($HT_{50}$) of SF/HA blend sponge treated groups were significantly decreased as compared with either those of SF or HA treatment group. Furthermore, SF/HA blend sponges significantly increased the size of epithelialization and collagen deposition as well as the number of PCNA positive cells on epidermal basement membrane as compared with those of control treatment. Especially, the 5 : 5 ratio group of SF/HA among all treatment groups was most effective on wound healing rate and histological studies. These results suggest that SF/HA blend sponges could accelerate the wound healing process through the increase of epithelialization, collagen deposition and basal cell proliferation in full thickness skin injury.

• Applied Microscopy
• /
• 제27권1호
• /
• pp.71-86
• /
• 1997

상처치유과정중 관상어류 피부 색소체계의 재분화경로를 규명하기 위하여 외부 색채가 화려한 담수산 금붕어 (Carassius auratus L.)를 실험재료로 하여 피부의 일정부위에 인위적인 상처를 유도한 후, 시간 경과에 따른 조직과 색소 체계의 치유과정을 고배율의 투과 전자현미경으로 관찰하였다. 금붕어 피부 색소세포는 정상조직에서 황색소세포, 백색소세포 그리고 혹색소세포 등 세 종류의 진피성 색소세포로 이루어져 있었다. 황색소세포에는 pterinosome과 carotenoid vesicle 등 두 종류의 색소과립이 분포되어 있었고, 백색소세포와 혹색소세포에는 무정형 색소결정인 leucosome과 전자밀도가 높은 구형의 색소과립인 melamosome이 각각 함유되어 있었다. 초기 상처치유반응은 상처 유도직 후에 표면 손상부위로 전이되는 상피세포와 혈구세포에 의하여 수행되었다. 상처 유도후 $5\sim7$일이 경과된 조직의 표본에서는 조면소포체가 특이하게 발달되어 진피성 색소세포의 공통 원기로 추정되는 세포의 출현이 확인되었다. 또한 재생된 조직내에서 재분화된 색소세포는 상처유도 후 3주가 경과된 표본에서 처음 관찰되었다. 색소과립의 재분화 경로는 세포 내에서의 색소과립 형성 과정과 마찬가지로 색소세포내에 잘 발달된 조면소포체와 골지체를 경유한 후, 분비소포의 형태로 생성림이 확인되었다. 그리고 재분화된 색소세포로 유입되는 색소 원기물질은 음세포과립을 통하여 수송되었는데, 특히 조면소포체가 풍부한 원기세포와 연접된 색소세포의 원형질막에서 매우 활발한 물질의 수송이 관찰되었다. 한편, 각 색소세포의 일차적인 재분화과정은 상처 유도후 4주가 경과되어 피부 상처가 치유되는 시점을 전후하여 완료되었으나, 재분화된 색소세포의 수나 분포밀도는 충분한 체색발현을 위한 상태에 비해 크게 미달되는 것으로 분석되었다. 따라서 특별한 환경의 변화가 없는 한, 상처유도 이전의 색소체계와 동일한 상태로의 복구에는 적어도 3개월 이상이 소요되는 것으로 확인되었다.