• Analytical Science and Technology
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• v.12 no.3
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• pp.218-223
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• 1999

A single use, screen-printed sensor for the measurement of liquid phase ethanol was developed and its electrochemical performance was investigated. Disposable type edthanol sensor was fabricated by serially screen printing the carbon paste, silverd pasted and insulator inlon a polyester substrate to pattern working and reference electrode sites and electrical contact. Alcohol dehydrogenase(ADH) or alcohol oxidase(AOD) together with appropriate electron transfer mediators was immobilized on the working electrode. To improve the sensitivity and reproducibility of carbon paste electrode, some pretreatment procedures were applied and their resultant electrochemical performance was examined. The disposable type electrochemical ethanol sensor developed in this study conveniently determines the ethanol in liquid samples such as blood and in fermentation process.

• Journal of Korean Society for Atmospheric Environment
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• v.16 no.6
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• pp.625-631
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• 2000

Practical devices for measuring personal exposure to nitrogen dioxide(NO$_2$) have been made for epidemiological studies of the health effects of air pollution Standard methods for NO$_2$ measurement such as the chemiluminescent method and Saltzman method are not suitable for personal exposure because they are heavy, large and complicated to operate. In this study, a passive monitor was tested for the measurements of indoor and outdoor NO$_2$ level. Through a comparative analysis of data sets obtained by on-site chemiluminescence system, we assessed the accuracy and precision of NO$_2$ passive monitors. We also examined the possibility of passive monitor in the study of indoor, outdoor and personal NO$_2$ exposure. The accuracy and precision of NO$_2$ passive monitor were analyzed assuming measurements of on-site chemiluminescence system is reference value and using duplicated measure- ments, respectively. From these analysis the NO$_2$ passive monitor was useful for measuring indoor, outdoor and personal exposure. And NO$_2$ level from on-site chemiluminescence systems could not properly represent the personal NO$_2$ exposure as well as indoor and outdoor level of ones house. Personal exposures were correlated more strongly with indoor NO$_2$ concentrations than with outdoor NO$_2$ concentrations. Since activity pattern of each person is different, it was considered that personal daily behavior and life-style might prevent the air pollutant exposure.

• Journal of fish pathology
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• v.22 no.1
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• pp.23-33
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• 2009

Vibrio harveyi was a significant pathogenic agent and cause a high mortality of cultured fish and shrimp in the aquaculture industry. In this study, we have investigated biochemical, physiological, serological and immunological characteristics of V. harveyi isolated from marine cultured fish. The phenotypes of V. harveyi were differentiated with their own biochemical characteristics and colors of colony on the TCBS agar. V. harveyi were classified into more than four serogroups by agglutination test. Most isolates were classified into a group A which is categorized with the same band pattern generated by western blotting. Group A was characterized by a major protein, which is ranged from 26 and 34 kDa in size, and has a virulence to oliver flounder more than reference strain KCCM40866. Oliver flounders vaccined with FKC of V. harveyi C05011 were highly resistant to infection by other strains of group A.

• Journal of the Korean Society for Library and Information Science
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• v.24
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• pp.171-204
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• 1993

The purpose of this study is to verify the hypothesis that the contents and range of library services are changed as the use of videotex systems increases. 1000 members of Chollian II were chosen as subjects for this study and the questionnaire was posted to them. The returned responses to questionnaire were analyzed with the statistical software packages 'SAS PC' and 'STATGRAPHICS'. The findings and conclusions of this study are summarised as follows: 1. Users of Chollian II make more use of videotex systems in seeking daily information than in seeking scholarly and professional information. It seems desirable to co-ordinate the services of videotex systems and libraries by redefining the traditional boundary of the two systems. Users will then conveniently get daily information through videotex servics, scholarly and professional information through the reference service of libraries. 2. Sex does not make any significant difference in the use of videotex systems. Most frequent use of videotex systems is made by those at the age of thirties. Businessmen and enterprisers make the most frequent use of videotex systems. Those of higher education are the best users. 3. As the use of videotex systems increases, use of newspapers and periodicals decreases while its increase rarely has influence on the use of library materials. 4. There is limited correlation between the use of videotex systems and that of library services. The increase of the use of videotex systems does not significantly increase or decrease the use of libraries. It changes, however, the pattern and methods of information services provided by libraries. 5. Users of special libraries make the most frequent use of videotex systems and there is not much difference in the use by users of other kinds of libraries.

• Geomechanics and Engineering
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• v.14 no.4
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• pp.337-344
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• 2018

The study of the mining effect influenced by a normal fault has great significance concerning the prediction and prevention of fault rock burst. According to the occurrence condition of a normal fault, the stress evolution of the working face and fault plane, the movement characteristics of overlying strata, and the law of fault slipping when the working face advances from footwall to hanging wall are studied utilizing UDEC numerical simulation. Then the inducing-mechanism of fault rock burst is revealed. Results show that in pre-mining, the in situ stress distribution of two fault walls in the fault-affected zone is notably different. When the working face mines in the footwall, the abutment stress distributes in a "double peak" pattern. The ratio of shear stress to normal stress and the fault slipping have the obvious spatial and temporal characteristics because they vary gradually from the higher layer to the lower one orderly. The variation of roof subsidence is in S-shape which includes slow deformation, violent slipping, deformation induced by the hanging wall strata rotation, and movement stability. The simulation results are verified via several engineering cases of fault rock burst. Moreover, it can provide a reference for prevention and control of rock burst in a fault-affected zone under similar conditions.

• Journal of the Korean Institute of Telematics and Electronics
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• v.27 no.2
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• pp.55-64
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• 1990

This paper concerned about a study on the direct pole placement PID self-tuning controller design for DC servo motor control system. The method of a direct pole placement self-tuning PID control for a DC servo motor of Robot manipulator tracks a reference velocity in spite of the parameters uncertainties in nonminimum phase system. In this scheme, the parameters of classical controller are estimated by the recursive least square (RLS)identification algorithm, the pole placement method and diophantine equation. A series of simulation in which minimum phase system and nonminimum phase system are subjected to a pattern of system parameter changes is presented to show some of the features of the proposed control algorithm. The proposed control algorithm which shown are effective for the practical application, and experiments of DC servo motor speed control for Robot manipulator by a microcomputer IBM-PC/AT are performed and the results are well suited.

• The Journal of the Acoustical Society of Korea
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• v.15 no.1
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• pp.54-60
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• 1996

In most pattern recognition and image understanding applications, images are degraded by noise and other distortions. Therefore, it is more relevant to decide how similar two objects are rather than to decide whether the two are exactly the same. In this paper, we propose a method for recognizing degraded symbols using a distance measure between two graphs representing the symbols. a symbol is represented as a graph consisting of nodes and edges based on the run graph concept. The graph is then transformed into a reference model graph with production rule containing the embedding transform. The symbols are recognized by using the distance measure which is estimated by using the number of production rules used and the structural homomorphism between a transformed graph and a model graph. the proposed approach is applies to the recognition of non-note musical symbols and the result are given.

• Food Science of Animal Resources
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• v.35 no.5
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• pp.692-702
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• 2015

Lactobacillus mucosae is a natural resident of the gastrointestinal tract of humans and animals and a potential probiotic bacterium. To understand the global protein expression profile and metabolic features of L. mucosae LM1 in the early stationary phase, the QExactive^TM Hybrid Quadrupole-Orbitrap Mass Spectrometer was used. Characterization of the intracellular proteome identified 842 proteins, accounting for approximately 35% of the 2,404 protein-coding sequences in the complete genome of L. mucosae LM1. Proteome quantification using QExactive^TM Orbitrap MS detected 19 highly abundant proteins (> 1.0% of the intracellular proteome), including CysK (cysteine synthase, 5.41%) and EF-Tu (elongation factor Tu, 4.91%), which are involved in cell survival against environmental stresses. Metabolic pathway annotation of LM1 proteome using the Kyoto Encyclopedia of Genes and Genomes (KEGG) database showed that half of the proteins expressed are important for basic metabolic and biosynthetic processes, and the other half might be structurally important or involved in basic cellular processes. In addition, glycogen biosynthesis was activated in the early stationary phase, which is important for energy storage and maintenance. The proteogenomic data presented in this study provide a suitable reference to understand the protein expression pattern of lactobacilli in standard conditions

• Korean Journal of Microbiology
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• v.26 no.3
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• pp.262-269
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• 1988

In order to investigate the virulence of Y. enterocolitica isolated in Korea, all necessary experiments were done including several virulence determinating tests-autoagglutination test, calcium-dependency test, HeLa cell invasion test, Sereny test, crystal violet binding test, and electrophoresis for plasmid pattern. The obtained results are as follows: The virulent strains of Y. enterocolitica revealed positive reactions on autoagglutination test, cacium-dependency test, and drystal violet binding test, while the avirulent strains did not. A positive reaction was observed only at $37^{\circ}C$ implying that the expression of virulence is temperature-dependent. In Sereny test, the standard reference virulent strain (serotype 0:8) showed positive reactions while the virylent experimental strains (serotype 1:3, 0:9) revealed negative results, which indicates that the virulence of Y. enterocolitica in experimental animals varied according to their serotypes. Most of the virulent strains contained 36-38Mdal plasmids, but the avirulent strains did not. In addition, it was noted that autoagglutination, calcium-dependency, and crystal violet binding were related to the presence of plasmids.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.36 no.6
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• pp.654-660
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• 2003

To evaluate the biological diversity of fish pathogenic streptococci, 35 strains isolated from diseased olive flounder (Paralichtys olivaceus), were analyzed using a random amplified polymorphic DNA (RAPD) technique with the oligonucleotide commercial primer 6 (Amersham Biosciences). Api 20 Strep test, drug resistance and artificial infection were carried out for further characterization of the isolates. RAPD fingerprints showed similar pattern in 25 strains (about $71.4\%$ of 35 isolates) and these strains were designed as RA group 1. Similarities greater than $44\%$ were obtained when the Dice coefficient was applied among the isolates of RA 1. On the other hand, the reference Streptococcus iniae showed a similar RAPD profile to the isolates with similarity levels of $40-93.3\%.$ Rh I was suggested to be the dominant group isolated from olive flounder suffering from streptococcosis. However, the isolates of Rh 1 group were not classified into the same species by the Api 20 Strep identification system. There was no peculiarity in drug resistance patterns of Rh I group isolates against 7 antibacterial agents. However, only 3 of 25 isolates $(0.12\%)$ showed oxytetracycline (OTC) resistance and OTC might be a useful chemotherapeutic agent in controlling the streptococcosis by strains of RA I group in olive flounder. Fish injected intraperitoneally with $10^5$ CFU of an isolate of Rh I and RA III group showed $60\%\;and\;50\%$ accumulative mortality for 20 days, respectively ($20\%$ in control or Rh II). However luther comparative studies about differences in virulence between isolates are needed.