• Journal of The Korean Society of Grassland and Forage Science
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• v.28 no.4
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• pp.273-280
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• 2008

In order to develop a simple and reproducible protocol for red top bentgrass (Agrostis alba L.), effect of different growth regulators was investigated for embrogenic calli induction and subsequent plant regeneration using mature seeds. MS medium containing 2 mg/L 2,4-D was optimal for embryogenic callus induction from mature seeds. The highest plant regeneration frequency (64.4%) was showed when the embryogenic callus tissues were cultured on N6 medium supplemented with 0.5 mg/L 2,4-D and 2 mg/L BA. Regenerated plantlets were grown normally when shoots transplanted to the soil. A high-frequency and efficient regeneration system from mature seeds would be helpful for molecular breeding of new variety of red top bentgrass through Agrobacterium-mediated genetic transformation.

• Journal of The Korean Society of Grassland and Forage Science
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• v.23 no.3
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• pp.187-192
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• 2003

This study was conducted to investigate dry matter yield and quality of six turf grasses, and thus to apply its result into making mixed species in the mixtures. The six turf grasses were Kentucky bluegrass(Newport), red fescue(Salem), creeping bentgrass(Crenshaw), perennial ryegrass(Palmer Ⅱ), tall fescue(Rebell Jr.) and redtop(Barricuda). This experiment was carried out for 3-years at grassland experimental field of Chungnam National University. The dry matter(DM) yield obtained in tall fescue and redtop was higher than those of the other species(p<0.05). Consideration of crude protein content and dry matter digestibility(DMD), the species of high quality compared to the other species were creeping bentgrass, perennial ryegrass, and Kentucky bluegrass. The crude protein dry matter yield was higher in Kentucky bluegrass and redtop, it was lower in red fescue(p<0.05). On the other hand, digestible dry matter(DDM) yield was higher in tall fescue and redtop, but lower in creeping bentgrass and red fescue(p<0.05). Based on the result mentioned above, therefore, it is suggested that turf grasses such as tall fescue, Kentucky bluegrass and redtop are recommended as species of turf grass which could be used as bottom grasses in mixtures.