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http://dx.doi.org/10.5333/KGFS.2008.28.4.273

Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration from Mature Seeds of Red Top Bentgrass (Agrostis alba L.)  

Park, Choong-Hoon (Division of Applied Life Science (BK21), Gyeongsang National University)
Kim, Kyung-Hee (Division of Applied Life Science (BK21), Gyeongsang National University)
Lee, Dong-Gi (Division of Applied Life Science (BK21), Gyeongsang National University)
Alam, Iftekhar (Division of Applied Life Science (BK21), Gyeongsang National University)
Lee, Ki-Won (Division of Applied Life Science (BK21), Gyeongsang National University)
Lee, Sang-Hoon (National Institute of Animal Sciences, RDA)
Kim, Ki-Yong (National Institute of Animal Sciences, RDA)
Won, Sung-Hye (Division of Applied Life Science (BK21), Gyeongsang National University)
Lee, Byung-Hyun (Division of Applied Life Science (BK21), Gyeongsang National University)
Publication Information
Journal of The Korean Society of Grassland and Forage Science / v.28, no.4, 2008 , pp. 273-280 More about this Journal
Abstract
In order to develop a simple and reproducible protocol for red top bentgrass (Agrostis alba L.), effect of different growth regulators was investigated for embrogenic calli induction and subsequent plant regeneration using mature seeds. MS medium containing 2 mg/L 2,4-D was optimal for embryogenic callus induction from mature seeds. The highest plant regeneration frequency (64.4%) was showed when the embryogenic callus tissues were cultured on N6 medium supplemented with 0.5 mg/L 2,4-D and 2 mg/L BA. Regenerated plantlets were grown normally when shoots transplanted to the soil. A high-frequency and efficient regeneration system from mature seeds would be helpful for molecular breeding of new variety of red top bentgrass through Agrobacterium-mediated genetic transformation.
Keywords
Callus; Plant regeneration; Red top bentgrass;
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Times Cited By KSCI : 6  (Citation Analysis)
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1 박병훈. 2005. 초사료자원학. 향문사. pp.53-59
2 이기원, 이동기, Nagib Ahsan,원성혜,이상훈, 김기용, 이병현. 2007. 식물생장조절물질이 페레니얼 라이그래스(Lolium perenne L.)의 캘러스 유도와 식물체 재분화에 미치는 영향. 한국초지학회지 27:235-240   과학기술학회마을   DOI
3 이상훈, 이동기, 이병현. 2004. 오차드그래스의 종자유래 캘러스배양 및 재분화에 미치는 배지 첨가물질의 영향. 한국작물과학회지 49(3):232- 236
4 이상훈, 이동기, 김진수, 이병현. 2003. 오차드그래스 성숙종자로부터 캘러스 유도 및 고효율 식물체 재분화. 한국식물생명공학회지 30(4):341- 346   DOI
5 Denchev, P.D., D.D. Songstad and J.K. McDaniel. 1997. Transgenic orchardgrass (Dactylis glomerata L.) plants by direct embryogenesis from micro- projecticle bombarded leaf cells. Plant Cell Rep. 16:813-819   DOI
6 Forster, J.W. and G. Spangenberg. 1999. Forage and turf grass biotechnology: principles, methods and prospects In: setlow, J. K.(Eds.), Genetic engineering: principles and methods. Kluwer Academic Publishers, New York, pp. 21-191
7 George, E.F., D.J.M. Puttock and H.J. George. 1987. Effect of medium acidity on growth and rooting of different plant growing in vitro. Plant Cell Tissue Cult. Org. Cult. 30(4):171-179   DOI
8 McKersie, B.D. 1997. Improving forage production systems using biotechnolgy. In: McKersie, B.D. and D.C.W. Brown (Eds.), Biotechnology in Agriculture Series, No. 17, CAB International, Wallingford, p. 3
9 Spangenberg, G., Z.Y. Wang and Potrykus. 1998. Biotechnology in forage and turf grass improve- ment. Frankel, R. and E. Galunl. Monographs on theoratical and applied genetics. Springer Verlag, Heidelberg, pp. 23; 192-221
10 Wang, G.R., H. Binding and U.K. Posselt. 1997. Fertile transgenic plants from direct gene transfer to protoplasts of Lolium perenne L. and Lolium multiflorum Lam. J. Plant Physiol. 151:83-90   DOI   ScienceOn
11 Naoko, T.O., K. Sukeo, O. Toshio, S.I. Itagaki, M. Onozawa and S. Masumi. 2006. Comparison of the abilities of redtop (Agrostis alba L.) and zoysiagrass (Zoysia japonica Steud.) to purify liquid animal waste. Grassland Sci. 52:15-21   DOI   ScienceOn
12 Jayaraj, J., G.H. Liang, S. Muthukrishnan and Z.K. Punja. 2008. Generation of low copy number and stably expressing transgenic creeping bentgrass plants using minimal gene cassette bombardment. Biol. Plant. 52(2):215-221   DOI
13 van der Mass, H.M., E.R. de Jong, S. Rueb, L.A.M. Hensgens and F.A. Krens. 1994. Stable transformation and long-term expression of the gusA reporter gene in callus lines of perennial ryegrass (Lolium perenne L.). Plant Mol. Biol. 24:401-405   DOI
14 이기원, 이상훈, 이동기,우현숙,김도현,최명석, 김기용, 이효신, 이병현. 2005a. 오차드그래스의 종자배양에 있어서 식물생장조절물질과 항산화제가 캘러스유도와 식물체 재분화에 미치는 영향. 한국초지학회지 25:191-198   과학기술학회마을   DOI
15 Murashige, T. and F. Skoog. 1962. A revised medium for rapid growth and bioassays with tobacco tissue cultures. Plant Physiol. 15:473-497   DOI
16 이기원, 이상훈, 이동기,우현숙,김도현,최명석,원성혜,서성, 이병현. 2005b. 켄터기 블루그래스에 있어서 캘러스 배양 및 식물체 재분화에 미치는 요인의 영향. 한국동물자원과학회지 47(6): 1067-1074   DOI   ScienceOn
17 Cheng, M., J.E. Fry, S. Plang, H. Zhou, C.M. Hironaka, D.R. Duncan, T.W. Conner and Y. Wan. 1997. Genetic transformation of wheat mediated by Agrobacterium tumefaciens. Plant Physiol. 115:971-980   DOI
18 Asano, Y. and M. Ugaki. 1994. Transgenic plants of Agrostis alba obtained by electroporation- mediated direct gene transfer into protoplasts. Plant Cell Rep. 13:243-246   DOI
19 이기원, 이상훈, 김도현,이동기,원성혜,이효신, 이병현. 2006. 캘러스의 형태와 항산화물질 첨가가 톨 페스큐의 식물체 재분화와 형질전환효율에 미치는 영향. 한국초지학회지 26:77-82   과학기술학회마을   DOI
20 Chu, C.C., C.S. Wang, C.C. Sun, C. Hsu, K.C. Yin, C.Y. Chu and F.Y. Bi. 1975. Establishment of an efficient medium for anther culture of rice through comparative experiments on the nitrogen sources. Scienta Sinic. 18:659-666
21 Fu, D., B. Huang, Y. Xiao, S. Muthukrishnan and G.H. Liang. 2007. Overexpression of barley hva1 gene in creeping bentgrass for improving drought tolerance. Plant Cell Rep. 26:467-477   DOI
22 우현숙, 이상훈, 이동기, 김진수,원성혜,이병현. 2004. 이탈리안 라이그래스의 성숙종자 유래 캘러스로부터 효율적인 식물체 재분화. 한국식물생명공학회지 31(1):43-48   DOI