Journal of The Korean Society of Grassland and Forage Science (한국초지조사료학회지)

The Korean Society of Grassland and Forage Science (한국초지조사료학회)
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Effect of Plant Growth Regulators on Callus Induction and Plant Regeneration from Mature Seeds of Red Top Bentgrass (Agrostis alba L.)

식물생장조절물질이 레드 톱 밴트그래스 (Agrostis alba L.)의 종자로부터 캘러스 유도와 식물계 재분화에 미치는 영향

  • Park, Choong-Hoon (Division of Applied Life Science (BK21), Gyeongsang National University) ;
  • Kim, Kyung-Hee (Division of Applied Life Science (BK21), Gyeongsang National University) ;
  • Lee, Dong-Gi (Division of Applied Life Science (BK21), Gyeongsang National University) ;
  • Alam, Iftekhar (Division of Applied Life Science (BK21), Gyeongsang National University) ;
  • Lee, Ki-Won (Division of Applied Life Science (BK21), Gyeongsang National University) ;
  • Lee, Sang-Hoon (National Institute of Animal Sciences, RDA) ;
  • Kim, Ki-Yong (National Institute of Animal Sciences, RDA) ;
  • Won, Sung-Hye (Division of Applied Life Science (BK21), Gyeongsang National University) ;
  • Lee, Byung-Hyun (Division of Applied Life Science (BK21), Gyeongsang National University)
  • Published : 2008.12.31
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Abstract

In order to develop a simple and reproducible protocol for red top bentgrass (Agrostis alba L.), effect of different growth regulators was investigated for embrogenic calli induction and subsequent plant regeneration using mature seeds. MS medium containing 2 mg/L 2,4-D was optimal for embryogenic callus induction from mature seeds. The highest plant regeneration frequency (64.4%) was showed when the embryogenic callus tissues were cultured on N6 medium supplemented with 0.5 mg/L 2,4-D and 2 mg/L BA. Regenerated plantlets were grown normally when shoots transplanted to the soil. A high-frequency and efficient regeneration system from mature seeds would be helpful for molecular breeding of new variety of red top bentgrass through Agrobacterium-mediated genetic transformation.

레드 톱 밴트그래스의 성숙종자로부터 최적조직배양조건을 확립하기 위하여 종자로부터 배발생 캘러스 유도 및 식물체 재분화 체계를 확립하였다. 종자로부터 배발생 캘러스 유도시 첨가되는 auxin으로는 2,4-D가 가장 효율적이었으며, 2 mg/L의 2,4-D가 첨가된 MS 배지에서 가장 높은 빈도로 배발생 캘러스가 유도되었다. 배발생 켈러스로부터 식물체 재분화는 0.5mg/L의 2,4-D와 2 mg/L의 BA가 첨가된 N6 배지에서 배양했을 때 64.4%의 높은 재분화율을 나타내었다. 본 연구를 통하여 확립된 성숙종자로부터의 고효율 재분화 시스템은 유전자 형질전환기술을 이용한 신품종 분자육종기술개발에 유용하게 이용 될 수 있을 것이다.

Keywords

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