• 한국어류학회지
• /
• 제30권4호
• /
• pp.194-204
• /
• 2018

본 연구는 매년 C. polykrikoides 적조에 의해 발생하는 양식 어류 폐사의 피해를 최소화하기 위해 적조시기 참돔의 절식과 밀도의 영향을 조사하였다. 해상가두리($11m{\times}11m{\times}5m$)에서 섭식-고밀도(F-HD), 절식-고밀도(S-HD), 절식-저밀도(S-LD) 세 그룹으로 구분하여 5주간의 절식 후 4주간의 먹이공급을 통해 참돔의 생존율, 성장, 성장회복 그리고 혈액학적 변화를 조사한 결과, 생존율은 F-HD에서 85.5%로 가장 낮았고, S-LD는 97.3%로 높은 생존율을 보였다. 5주간의 절식기간 동안 두절식구는 전장, 체중 성장률에서 마이너스 성장을 보여 섭식구와 유의한 차이를 보였다(P<0.05). 하지만 먹이 재공급 후두 절식구 모두 성장회복을 보였다. 특히 S-LD는 먹이 재공급 기간 동안 빠른 성장회복을 통해 보상성장 경향을 보였으며, 섭식구와 유의한 차이를 나타냈다(P<0.05). ALB, TCHO, TP, TG 혈액분석을 통한 실험구의 영양상태는 절식구에서 섭식구에 비해 유의하게 감소하였지만 먹이 공급 후 실험개시 시 수준으로 빠르게 회복하였다. 스트레스와 생리활성 지표인 Ht, Hb, AST, ALT 그리고 GLU가 적조 노출 후 F-HD에서 동일한 시기에 급격히 상승하여, 적조시기 섭식, 고밀도 사육환경은 양식어류에게 더 민감한 사육환경으로 작용할 것으로 여겨진다. 따라서 본 연구의 적조시기 절식과 밀도에 따른 생존율, 성장회복, 혈액성상을 통한 결과를 토대로, 적조 노출에 따른 절식과 밀도 조절이 사육어류 관리방안을 위한 기초자료로 활용될 수 있을 것이다.

• 한국해양공학회:학술대회논문집
• /
• 한국해양공학회 2002년도 추계학술대회 논문집
• /
• pp.251-255
• /
• 2002

Eutrophic matters accumulated on the bottom of sea, river and lake cause red tide phenomenon in ocean and outbreak green algae in river and lake. Systems are developed to remove the noxious deposit. But the existing systems remove not only the eutrophic matters but also natural materials, sand, pebbles etc. that should remain at the bottom. This paper describes a new system that can safely, and economically take away the noxious deposit in underwater. High pressure water jet is used to induce vortices in the triangular suction section, and air-lifting pump to lift up the deposit. The mixture of the water and deposit is filtered through the drum filters. An under camera shows the under water situation along the moving direction of the system that is controlled by a remote operator. This remote controlled moving system obliterate the necessity of the diver that usually costs high. The experimental results show the effectiveness of the suggested system.

• 한국환경과학회지
• /
• 제3권3호
• /
• pp.185-195
• /
• 1994

Masan bay is one of the polluted enclosed bays, which has red tides problem and the formation of oxygen deficient water in the bottom layer. Most important factors that cause eutrophication and red tide is nutrient materials containing nitrogen and phosphorus which stem from terrestrial sources and nutrients released from sediment. Therefore, to improve of water quality, reduction of these nutrient loads should be indispensible. At this study, the three-dimensional numerical hydrodynamic and eutrophication model, which were developed by Institute for Resources and Environment of Japan, were applied to analyze the processes affecting the phytoplankton production and also to evaluate the effect of water quality improvement plans on phytoplankton production. In field sorvey, the range of concentrations of chlorophyll-a at surface area was found to be 29.17 - 212.5mg/m3, which were exceeding eutrophication criteria. The constant currents defined by integrating the simulated tidal currents over 1 tidal cycle showed the counterclockwise eddies in the southern part of Budo. The general directions of constant currents were found to be southward at surface and northward at bottom over all the bay. The eutrophication model was calibrated with the data surveyed in the field area in June, 1993. The calculated results are in fairly good agreement with values within relative error of 30%. The pollutant load from the sources such as the input from terrestrial release from the sediment was reduced by the rate of 50, 70, 90, 98% to effect of phytoplankton production. Phytoplankton production was reduced to of the 90% reduction of the input loads from terrestrial sources and 8% in 90% reduction of the load from sediment.

• 한국양식학회지
• /
• 제11권3호
• /
• pp.285-294
• /
• 1998

When Cochlodinium polykrikoides came into the culture tanks through influent cultivated water during the red tides, hundred thousands of commercial flounders were concomitantly killed and many culturists suffered from a great deal of financial loss in the east coast of Korea. It is charactrized by high sinking rate after sunset and the formatino of clump which results in oxygen deficiency by its respiration at tank bottom under condition. We investigated the efficacy of hydrogen peroxide and chlorine dioxide, known to form radicals, for extermination of red tide organism C. polykrikoides. When C. polykrikoides seawater with a density of 6,000 cells/$m\ell$ was treated with 14, 28 and $42mg/\ell$ of hydrogen peroxide, its survival rate was markedly decreased to 9.8, 0.8 and 0.3% respectively immediately after 6 hours of treatments whereas when it was treated with 1.5, 2.1 and $3.0mg/\ell$ chlorine dioxide, its survival rate showed 87.7, 81.3 and 80.1 and 80.1% respectively at the same treatment time. Hydrogen peroxide was the effective agent since it has scarcely injured the cultured olive flounder when exposed to the tested concentration range of $14~28mg/\ell$ with the extermination of almost3 C. polykrikoides during the experimental period of 5 days and has shown the oxygen increase of approximately $1.23mg/\ell$ 2 hours immediately after the flounder by C. polykrikoides in the land-based culture tank is assumed to be not by the toxicity of itself but by oxygen dificiency from the rapid respiration of dinoflagellate clump sunken to the tank bottom.

• ALGAE
• /
• 제21권3호
• /
• pp.311-316
• /
• 2006

The effects of light quality and irradiance on the growth of Cochlodinium polykrikoides were investigated in the laboratory. At 25°C and 30 psu the irradiance-growth curve was described as μ = 0.34 (I-9.76)/(I+12.5), (r=0.98). This suggests half-saturation photon flux density (PFD) (Ks) of 32.0 μmol photons m–2 s–1, and a compensation PFD (Ic) of 9.76 μmol photons m–2 s–1. Because the Ic equates to a depth of ca. 15.4 m, these responses suggest that irradiance at the depth around and below the thermocline in Yeosuhae Bay would provide favorable conditions for C. polykrikoides. Photoinhibition did not occur at 300 μmol photons m–2 s–1, which was the maximum irradiance used in this study. Blue (450 nm), yellow (590 nm) and red (650 nm) light had different effects on the growth of C. polykrikoides: it grew well under blue light, but not under yellow light. This implies that C. polykrikoides is more likely to cause an outbreak of red tide in the open sea where blue-green wavelengths predominate, rather than in enclosed water bodies where suspended particles absorb most of the blue wavelengths, and yellow-orange wavelengths predominate.

• 한국환경과학회지
• /
• 제17권4호
• /
• pp.439-449
• /
• 2008

Algicidal bacterium was isolated from sea water during the declining period of Cochlodinium polykrikoides blooms and this bacterium had a significant algicidal activity against C. polykrikoides. In this study, algicidal bacterium was identified on the basis of biochemical and chemotaxonomic characteristics, and analysis of 16S rDNA sequences. The algicidal bacterium showed 98.6% homology with Micrococcus luteus ATCC $381^T$. Therefore, this bacterium was designated Micrococcus luteus SY-13. The optimal culture conditions of the algicidal bacterium was $25^{\circ}C$, initial pH 8.0, and 3.0% NaCl concentration. M. luteus SY-13 is assumed to produce secondary metabolites which have algicidal activity. When 10% culture filtrate of this strain was applied to C. polykrikoides ($1.0\;{\times}\;10^4\;cells/ml$) cultures, over 98% of C, polykrikoides cells were destroyed within 6 hours. The culture filtrate of M. luteus SY-13 exhibited similar algicidal activity after heat-treatment at $121^{\circ}C$ for 15 min. While algicidal activity remained in filtrates with pH adjusted to 8.0, loss of algicidal activity occurred when the pHs of filtrates were adjusted to over 9.0 or heat-treated at $121{\times}180^{\circ}C$ for 1 hour. M. luteus SY-13 showed significant algicidal activities against C. polykrikoides (98.9%) and a wide algicidal range against various harmful algal bloom (HAB) species. However, there was no algicidal effect on diatom and marine livefood organisms except Isocrysis galbana. These results suggest that M. luteus SY-13 could be a candidate for use in the control of HABs.

• Environmental Analysis Health and Toxicology
• /
• 제25권4호
• /
• pp.273-278
• /
• 2010

Objectives : This study was aimed to examine the acute toxicity assessment of two new algicides, thiazolidinediones derivatives (TD53 and TD49), which were synthesized to selectively control red tide, to the marine ecosystem. Methods : The assessment employed by a new method using Ulva pertusa Kjellman which has been recently accepted as a standard method of ISO. The toxicity was assessed by calculating the $EC_{50}$ (Effective Concentration of 50%), NOEC (No Observed Effect Concentration) and PNEC (Predicted No Effect Concentration) using acute toxicity data obtained from exposure experiments. $EC_{50}$ value of TD49 and TD53 was examined by 96-hrs exposure together with Solutol as a TD49 dispersing agent and DMSO as a TD53 solvent. Results : $EC_{50}$ value of TD53 was $1.65\;{\mu}M$. From the results, values of NOEC and PNEC were calculated to be $0.63\;{\mu}M$ and 1.65 nM, respectively. DMSO under the range of $0{\sim}10\;{\mu}M$, which is same solvent concentration used in examining TD53, showed no toxic effect. $EC_{50}$ value of TD49 was $0.18\;{\mu}M$ and that of Solutol was $1.70\;{\mu}M$. NOEC and PNEC of TD49 were $0.08\;{\mu}M$ and 0.18 nM, respectively and those for Solutol were $1.25\;{\mu}M$ and 1.25 nM, respectively. Conclusions : From the values of NOEC, PNEC of TD53 and TD49, TD49 showed 9 times stronger toxicity than TD53. On the other hand, DMSO showed no toxicity on the Ulva pertusa Kjellman, but Solutol was found to be a considerable toxicity by itself.

• 한국해양학회지:바다
• /
• 제17권1호
• /
• pp.9-15
• /
• 2012

전세계적으로 연안해역에서 확산되고 있는 유해유독성 식물플랑크톤의 대발생은 수산자원생물자원에 심각한 피해를 입힌다. 본 연구에서는 유해성 미세조류 대발생을 제어하기 위해 개발된 신물질 Thiazolidinedione 유도체(TD49)의 살조능을 유해성 미세조류 성장단계(초기성장기, 대수증식기, 안정기)에 따라 조사하였다. TD49는 $Heterosigma$ $akashiwo$, $Chattonella$ $marina$ 그리고 $Chattonella$ sp.의 세포를 사멸시켰으며, 특히 낮은 농도($0.02{\mu}M$)의 TD49는 대수증식기와 안정기보다 초기 성장기에서 우수한 살조효과를 보였다. 또한 모든 성장단계에서 유해생물을 제어 할 수 있는 TD49의 농도는 $2{\mu}M$로 측정되었다. 무각 편모조류인 $Heterosigma$ $akashiwo$, $Chattonella$ $marina$ 그리고 $Chattonella$ sp.은 세포벽이 약하여 TD49물질에 의해 세포가 쉽게 파괴되어 우수한 살조효과를 보였다. 결과적으로 본 연구에서 개발된 Thiazolidinedione 유도체(TD49)는 유해적조생물 $H.$ $akashiwo$, $C.$ $marina$ 그리고 $Chattonella$ sp.를 제어할 수 있는 우수한 물질로 판단되었으나, 추후 현장 실용을 위해 메소코즘과 같은 인공생태계를 이용한 해양생태계 위해성 평가가 체계적으로 추진되어야 한다.

• 해양환경안전학회지
• /
• 제16권2호
• /
• pp.141-151
• /
• 2010

우리나라에서는 적조가 발생하면 적조발생 해역에 황토를 살포하고 있다. 황토가 살포되는 해역은 대부분 가두리 양식장 주변이며 매년 권장 살포량이상으로 대량 살포되고 있다. 본 연구에서는 지금까지 적조가 발생하여 매년 황토가 살포되어온 해역과 황토가 살포되지 않은 해역을 대상으로 추계(2008년 10월)와 춘계(2009년 4월)에 플랑크톤 네트에 의한 채집과 ADCP에 의한 체적산란강도를 계측하여 플랑크톤 분포특성과 플랑크톤의 분포밀도를 조사하였다. 황토를 살포하지 않은 해역에서 채집된 생물의 종수 및 단위체적당 개체수는 높았으나, 황토를 살포한 해역에서는 낮았다. 각 정점에서 채집한 플랑크톤의 종별, 체장별 개체수와 분포밀도의 평균치를 이용한 체적산란강도 $SV_c$와 ADCP에 의해 계측된 체적산란강도 $SV_m$를 비교해 본 결과, 황토를 살포하지 않은 해역의 $SV_c$와 $SV_m$는 거의 일치하였으나, 황토를 살포한 해역의 $SV_m$는 $SV_c$보다 4.3 dB 높았다. 즉 황토를 살포한 해역에서는 부유물이 ADCP에 크게 영향을 미치고 있음을 확인하였다. ADCP에 의해 계측된 체적산란강도의 수평분포도에서 체적산란강도는 황토를 살포한 해역이 황토를 살포하지 않은 해역보다 높았으며, 봄철이 가을철보다 높았다. 또한 체적산란강도 추정에 ADCP를 이용하면 채집에 의한 분포밀도의 과대 또는 과소평가를 방지할 수 있음을 확인할 수 있었다.

• 해양환경안전학회:학술대회논문집
• /
• 해양환경안전학회 2006년도 추계학술발표회
• /
• pp.97-104
• /
• 2006

선박과 항만 터미널에 의한 오염에 대하여 항만 환경의 보호를 위해 강력한 규제지침을 적용해야한다. 불법적인 선박의 밸러스트수 교환으로 발생하고 연안의 항내에 들어와서 교환을 하면 식물과 동물의 외래종 침입하여 먹이 사슬을 파괴하는 원인이 된다. 질소가 풍부한 물의 교환은 미생물의 성장을 촉진시켜 연안에 적조를 유발시킨다. 또한 항만의 나쁜 영향을 미치는 것은 선박과 물동량을 수송하는 트럭에서 다량의 디젤 산화물을 대기로 배출한다. 국제해사기구 (International Maritime Organization: IMO) 에서는 선박에서 발생되는 오염물질 둥에 대한 규제강화를 위하여 최근 새로운 해사환경협약의 채택 및 발효를 강력히 추진하고 있다. 우리나라는 국제해사기구의 A 그룹 이사국으로서의 국제적인 위상과 해양환경 보호를 위하여 현재 발효되고 추진 중에 있는 협약들에 대한 연구와 대처를 효과적으로 하여야 한다. 이 논문은 해양관련 환경규제협약인 대기오염방지협약, 선박의 유해방오도료 사용규제협약, 밸러스트수 배출규제협약, 선박으로부터 오수에 의한 오염방지를 위한 협약 풍의 주요 현안을 파악하고 분석하여 이에 따른 대응책을 제시하고자 한다.