• 한국막학회:학술대회논문집
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• 한국막학회 2004년도 Proceedings of the second conference of aseanian membrane society
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• pp.55-58
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• 2004

Cell separation from peripheral blood was investigated using surface-modified polyurethane (PU) membranes with different functional groups. Both red blood cells and platelets could pass through unmodified PU and PU-SO$_3$H membranes, while the red blood cells preferentially passed through PU-N(C$_2$H$_{5}$ )$_2$ and PU-NHC$_2$H$_4$OH membranes. The permeation ratio of T and B cells was less than 25% for the surface-modified and unmodified PU membranes. CD34$^{+}$ cells have been recognized as various kinds of stem cells including hematopoietic and mesenchymal stem cells. The adhesiveness of CD34$^{+}$ cells on the PU membranes was found to be higher than that of red blood cells, platelets, T cells or B cells. Overall, the adhesiveness of blood cells on the PU membranes increased in the following order: red blood cells $\leq$ platelets ＜ T cells $\leq$ B cells ＜ CD34$^{+}$ cells. Treatment of PU-COOH membranes with a human albumin solution to detach adhered blood cells, allowed recovery of mainly CD34$^{+}$ cells in the permeate, while both red blood cells and platelets could be isolated in the permeate using unmodified PU membranes. The PU membranes showed different permeation and recovery ratios of specific cells depending on the functional groups attached to the membranes.mbranes.

• 대한미생물학회지
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• 제18권1호
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• pp.67-71
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• 1983

Antisera to ox red blood cells were prepared by injection of ox red blood cell stroma without adjuvant in outbred white rabbits. Purified IgM fraction for T-cell subset assay was obtained from these rabbit anti-ox red blood cell stroma antisera by precipitation with 50% saturated ammonium sulphate followed by DEAE-Cellulose chromatography and Sephadex G-200 gel filtration. The serological identification of purified IgM fraction was achieved by immunoelectrophoresis with guinea pig antiserum against rabbit anti-ox red blood cell IgM antibody.

• 대한핵의학회지
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• 제1권1호
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• pp.1-19
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• 1967

The effects of X-ray irradiation and the thyroid gland on the erythropoietic system were studied in the white male rabbits. The total body irradiation was done in doses of 250 r and 500 r to each of 5 rabbits for 10days. The factors were 220KV, 10mA, FLI/4 Cu+1 mmAI(HVL:2.0 mm Cu) 50 cm F.S.D. The thyroid dysfunction was experimentally induced, by giving 2mg of thyroid tablets per kg body weight for 15 days in 5 rabbits for hyperthyroidism and by giving 1.5 mC of $^{131}I$ per kg body weight in another 5 rabbits for hypothyroidism. Fourteen healthy rabbits were used as control. The hematologic changes and ferrokinetic data obtained from $^{59}Fe$ and apparent half survival of the red blood cells obtained from $^{51}Cr$ were compared. Following were the results: A. X-ray irradiated group; 1. There were no significant changes in hematologic findings except for leucopenia. A slight decrease of red blood cells was observed in 500 r irradiated animals. 2. The decreases in the iron turnover rates of the plasma and red blood cells as well as in the red cell renewal rate were found in both groups. A :significant decrease of the red cell iron utilization rate was observed in the 500 r irradiated animals. 3. The apparent half survival times of the red blood cells were slightly, in the 250 r ($12.1{\pm}0.80$ days), and markedly shortened in the 500 r irradiated animals ($9.8{\pm}1.38$ days), the normal being $14.0{\pm}1.6$ days. 4. It appears, therefore, that the anemia caused by X-ray irradiation is due to the inhibition of hemopoietic function and the excess destruction of the red blood cells. B. Thyroid dysfunction group; 1. The slight increases of the red blood cell count and circulating blood volume with the normal serum iron level were observed in the hyperthyroid group, while the decreases of the red and white blood cell counts, hemoglobin and hematocrit values with a marked decrease of the serum iron level in the hypothyroid group. 2. A marked decrease of the plasma iron disappearance rate with increases of plasma iron turnover, red cell iron utilization and red cell iron turnover were observed in the hyperthyroid group, while the marked delay and decreases in the hypothyroid group. 3. The apparent half survival times of the red blood cells were almost the same with the control in the hyperthyroid group, ($14.0{\pm}1.58$ while a marked shortening in the hypothyroid group $10.6{\pm}0.30$. 4. It was reconfirmed that the thyroid hormones bear a close relationship with the erythropoietic system, namely, the latter is stimulated by the former. The lack of the thyroid hormones thus induces the bone marrow depression leading to anemia the major cause of which, therefore, is not hemolysis.

• 대한기계학회논문집B
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• 제32권11호
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• pp.856-862
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• 2008

This paper presents the characterization of a continuous magnetophoretic microseparator for separating white and red blood cells from peripheral whole blood cells based on their native magnetic properties. The magnetophoretic microseparator separated the blood cells using a high gradient magnetic separation (HGMS) method without the use of additives such as magnetic beads or probing materials. Experimental results show that the paramagnetic capture mode microseparator can continuously separate out 93.5% of red blood cells and 97.4% of white blood cells from diluted whole blood, and the diamagnetic capture mode microseparator can continuously separate out 89.7% of red blood cells and 72.7 % of white blood cells by using applying an external magnetic flux of 0.2 T using a permanent magnet.

• The Korean Journal of Physiology
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• 제24권1호
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• pp.15-26
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• 1990

Hemoglobin was purified from the outdated human red blood cells. Phospholipids were purified from egg yolk and stored in chloroform. The artificial red blood cells (hemosome) were prepared by encapsulation of hemoglobin with phospholipid mutilayer using rotary vacuum evaporator. The shape and size of hemosomes were measured by phase contrast microscope and image analyzer. The function of hemosomes was tested by measuring oxygen dissociation curve using blood gas analyzer. In order to test whether hemosomes are useful as blood substitute they were infused into rats of which one third of total blood were drawn. The results obtained are summarized at followings. 1) Hemosomes were spherical shape and their mean diameter was 0.7 um. 2) Oxygen dissociation curve of hemosomes showed the same figure as that of normal red blood cells. 3) All rats given 1/3 transfusion with hemosomes survived until sacrificed whereas three of four rats given 1/3 transfusion with saline died within 1 hour and the rest of them died within 24 hours.

• 한국자기학회지
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• 제24권1호
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• pp.28-33
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• 2014

최대 0.27 T 세기와 0.102 ms 펄스시간을 가지는 자기장 자극시스템을 이용하여 손에 인가한 후에 생혈액 분석을 통해 말초혈관에 흐르는 적혈구의 형태학적 변화를 조사하였다. 펄스자기장 자극 전 피실험자의 적혈구는 10여개 이상씩 서로 붙어있는 연전형성(連錢形成, rouleau formation)이 일어나서 적혈구의 움직임이 느려졌으나, 손에 10분간 자기장 인가한 후에 연전형성이 되었던 적혈구가 대부분 떨어져서 독립적인 움직임을 보이면서 운동이 활발해졌다. 또한 왼쪽 손에 펄스자기장을 10분 자극한 후에 자극받지 않은 오른쪽 손에서 채혈했을 때에도 연전현상이 똑같이 개선되었다. 이는 혈류흐름에 의해 체내에 모든 적혈구의 연전현상이 개선된 것으로 판단할 수 있다.

• International Journal of Vascular Biomedical Engineering
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• 제1권2호
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• pp.30-35
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• 2003

Flow characteristics of blood flow in a micro channel were investigated experimentally using a micro-PIV (Particle Image Velocimetry) velocity field measurement technique. The main objective of this study was to understand the real blood flow in micron-sized blood vessels. The Reynolds number based on the hydraulic diameter of micro-channel for deionized (DI) water was about Re=0.34. For each experimental condition, 100 instantaneous velocity fields were captured and ensemble-averaged to get the spatial distributions of mean velocity. In addition, the motion of RBC (Red Blood Cell) was visualized with a high-speed CCD camera. The captured flow images of nano-scale fluorescent tracer particles in DI water were clear and gave good velocity tracking-ability. However, there were substantial velocity variations in the central region of real blood flow in a micro-channel due to the presence of red blood cells.

• 대한한의학회지
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• 제26권4호
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• pp.143-151
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• 2005

Objectives: The purpose of this study was to investigate the effect of exterior vascular laser irradiation therapy by live blood analysis. Methods: We analysed the changing forms of the live blood sample with a microscope before and after exterior vascular laser irradiation therapy of the blood. Results: Rouleau of red cells, erythrocyte aggregation of red cells, thrombocyte aggregation, uric acid crystals, red crystals, and protoplasts in blood were decreased significantly after exterior vascular laser irradiation therapy. Conclusions: This study suggests that exterior vascular laser irradiation will have positive effects in eliminating various effete matters in blood and will have efficacy in the treatment and prevention of cardiovascular system disorders and hyperlipidemia, caused by effete matters, or numbness and arthralgia caused by blood stagnancy and blood circulation disorder.

• The Journal of the Acoustical Society of Korea
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• 제25권4E호
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• pp.159-165
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• 2006

Human, horse and rat bloods in a cylindrical chamber where flow was controlled by a stirring magnet were used for studying red blood cell aggregation. Ultrasound backscattered powers from blood were obtained from the backscattered signals measured by a 5 MHz focused transducer in a pulse-echo setup. The experimental results showed the differences in red blood cell (RBC) aggregation tendency among the three mammalian species with an order of horse > human > rat. The ultrasound backscattered power decreased with stirring speed in human and horse blood, but no variations were observed in rat blood. Sudden flow stoppage led to the slow increase of the backscattered power for human and horse blood. There was no self-aggregation tendency in rat blood. The enveloped echo images showed the spatial and temporal variations of RBC aggregations in the cylindrical chamber. These observations from the different mammalian species may give a better understanding of the mechanism of RBC aggregation.

• Journal of Animal Science and Technology
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• 제60권8호
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• pp.20.1-20.4
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• 2018

Platelet rich plasma (PRP) is popularly used in the horse industry to enhance regeneration of tissue injury that has limitation of blood supply. This study aimed to compare the methods for platelet rich plasma preparation since they has not been established yet. Blood was collected from six horses and platelets were concentrated by three different methods (2-step centrifugation, separated centrifugation and separated centrifugation using histopaque). Concentrated blood was analyzed using Advia hematology systems. In the result, separated centrifugation with histopaque showed the significantly lower number of red blood cells than other groups. The 2-step centrifugation showed the significantly higher number of white blood cells than other groups, while it contained the highest concentration of red blood cells among three groups. In the 2-step centrifugation, separated centrifugation and separated centrifugation with histopaque, platelets were concentrated 4.5, 5.3 and 5.6 times, respectively. And no significant difference of the platelet concentration between the three groups was found. This study demonstrated that separated centrifugation using histopaque was the best method for platelet rich plasma preparation because of the proper amount of platelets and the separation of red blood cells from platelet rich plasma.