Ultrasound Backscattering from Erythrocyte Aggregation of Human, Horse and Rat Blood under Rotational Flow in a Cylindrical Chamber

  • Nam, Kweon-Ho (Interdisciplinary Postgraduate Program in Biomedical Engineering, Cheju National University) ;
  • Paeng, Dong-Guk (Marine Industrial Engineering, Cheju National University) ;
  • Choi, Min-Joo (Department of Biomedical Engineering, College of Medicine, Cheju National University)
  • Published : 2006.12.30

Abstract

Human, horse and rat bloods in a cylindrical chamber where flow was controlled by a stirring magnet were used for studying red blood cell aggregation. Ultrasound backscattered powers from blood were obtained from the backscattered signals measured by a 5 MHz focused transducer in a pulse-echo setup. The experimental results showed the differences in red blood cell (RBC) aggregation tendency among the three mammalian species with an order of horse > human > rat. The ultrasound backscattered power decreased with stirring speed in human and horse blood, but no variations were observed in rat blood. Sudden flow stoppage led to the slow increase of the backscattered power for human and horse blood. There was no self-aggregation tendency in rat blood. The enveloped echo images showed the spatial and temporal variations of RBC aggregations in the cylindrical chamber. These observations from the different mammalian species may give a better understanding of the mechanism of RBC aggregation.

Keywords

References

  1. O. K. Baskurt and H. J. Meiselman, 'Blood rheology and hemodynmics', Semin. Thromb. Hernost., 29, 435-450, 2003 https://doi.org/10.1055/s-2003-44551
  2. H. Schmid-Schobein and E. Volger, 'Red-cell aggregation and red-cell deformability in diabetes', Diabetes, 25, 897-902, 1976
  3. S. M. Razavian, M. Del Pino, A. Simon and J. Levenson, 'Increase in erythrocyte disaggregation shear stress in hypertension', Hypertension, 20, 247-252, 1992 https://doi.org/10.1161/01.HYP.20.2.247
  4. A. Hadengue, S. M. Razavian, M. Del Pino, A. Simon and J. Levenson, 'Influence of sialic acid on erythrocyte aggregation in hypercholesterolemia', Thromb. Haemost, 76, 944-949, 1996 https://doi.org/10.1055/s-0038-1650690
  5. O. K. Baskurt, A. Temiz and H. J. Meiselman, 'Red blood cell aggregation in experimental sepsis', J. Lab. Clin. Med., 130, 183-190, 1997 https://doi.org/10.1016/S0022-2143(97)90094-9
  6. L. Zilberman, O. Roqowski, M. Rozenblat, I. Shapira, J. Serov, P. Halpern, I. Dotan, N. Arber and S. Berliner, 'Inflammation-related erythrocyte aggregation in patients with inflammatory bowel disease', Dig. Dis. Sci., 50, 677-683, 2005 https://doi.org/10.1007/s10620-005-2556-2
  7. F. J. Neumann, H. A. Katus, E. Hoberg, P. Roebruck, M. Braun, H. M. Haupt, H. Tillmanns and W. Kubler, 'Increased plasma viscosity and erythrocyte aggregation: indicators of an unfavourable clinical outcome in patients with unstable angina pectoris', Br. Heart J., 66, 425-430, 1991 https://doi.org/10.1136/hrt.66.6.425
  8. A. Chabanel, M. H. Horellou, J. Conard and M. M. Samama, 'Red blood cell aggregability in patients with a history of leg vein thrombosis: influence of post-thrombotic treatment', Br. J. Haematol., 88, 174-179, 1994 https://doi.org/10.1111/j.1365-2141.1994.tb04993.x
  9. M. Poggi, G. Palareti, R. Biagi, C. Legnani, M, Parenti, A. C. Babini, L. Baraldi and S. Coccheri, 'Prolonged very low calorie diet in highly obese subjects reduces plasma viscosity and red cell aggregation but not fibrinogen', Int. J. Obes. Relat. Metab. Disord., 18, 490-496, 1994
  10. M. M. Khan, R. R. Puniyani, N. G. Huilgol, M. A. Hussain and G. G. Ranade, 'Hemorheological profiles in cancer patients', Clin. Hemorheol., 15, 37-44, 1995
  11. K. Ohta, F. Gotoh, M. Tomita, N. Tanahashi, M. Kobari, T. Shinohara, Y. Tereyama, B. Mihara and H. Takeda, 'Animal species differences in erythrocyte aggregability', Am. J. Physiol., 262 (Heart. Circ. Physiol.), H1009-H1012, 1992 https://doi.org/10.1152/ajpcell.1992.262.4.C1009
  12. X. Weng, G. Cloutier, P. Pibarot and L.-G. Durand, 'Comparison and simulation of different levels of erythrocyte aggregation with pig, horse, sheep, calf, and normal human blood', Biorheology, 33, 365-377, 1996 https://doi.org/10.1016/0006-355X(96)00028-5
  13. O. K. Baskurt, R. A. Farley, and H. J. Meiselman, 'Erythrocyte aggregation tendency and cellular properties in horse, human, and rat: a comparative study,' Am. J. Physiol., 272 (Heart. Circ. Physiol.), H2604-H2612, 1997
  14. U. Wind berger, A. Bartholovitsch, R. Plasenzotti, K. J. Korak and G. Heinze, 'Whole blood viscosity, plasma viscosity and erythrocyte aggregation in nine mammalian species: reference values and comparison of data', Exp. Physiol., 88, 431-440, 2003 https://doi.org/10.1113/eph8802496
  15. S. Chen, G. Barshtein, B. Gavish, Y. Mahler and S. Yedgar, 'Monitoring of red blood cell aggregability in a flow-chamber by computerized image analysis', Clin. Hemorheol., 14, 497-508, 1994,
  16. T. L. Fabry, 'Mechanism of erythrocyte aggregation and sedimentation,' Blood, 70, 1572-1576, 1987
  17. C. Lacombe and J. C. Lelievre, 'Interpretation of rheograms for assessing RBC aggregation and deformability', Clin. Hemorheol., 7, 47-61, 1987
  18. K. K. Shung and D.-G. Paeng, 'Ultrasound: an unexplored tool for blood flow visualization and hemodynamic measurements', Jpn J. Appl. Phys., 42, 2901-2908, 2003 https://doi.org/10.1143/JJAP.42.2901
  19. B. Sigel, J. Machi, J. C. Beitler and J. R. Justin, 'Red cell aggregation as a cause of blood-flow echogenicity', Radiology, 148, 799-802, 1983 https://doi.org/10.1148/radiology.148.3.6878705
  20. D. - G. Paeng, P. J. Cao and K. K. Shung, 'Doppler power variation from porcine blood under steady and pulsatile flow', Ultrasound Med. Biol., 27, 1245-1254, 2001 https://doi.org/10.1016/S0301-5629(01)00405-7
  21. D. - G. Paeng and K. K. Shung, 'Cyclic and radial variation of the doppler power from porcine whole blood', IEEE Trans Ultrason Ferroelec. Freq. Control, 50, 614-622, 2003 https://doi.org/10.1109/TUFFC.2003.1209548
  22. D. - G. Paeng, R. Y. Chiao and K. K. Shung, 'Echogenicity variations from porcine blood I: the bright collapsing ring under pulsatile flow', Ultrasound Med. Biol., 30, 45-55, 2004 https://doi.org/10.1016/j.ultrasmedbio.2003.08.015
  23. G. Cloutier, M. Daronatand, D. Savery, D. Garcia, L. G. Durand and F. S. Foster, 'Non-Gaussian statistics and temporal variations of the ultrasound signal backscattered by blood at frequencies between 10 and 58 MHz', J. Acoust. Soc. Am., 116, 566-577, 2004 https://doi.org/10.1121/1.1760791
  24. D. Fatkin, T. Loupas, J. Low and M. Feneley, 'Inhibition of red cell aggregation prevents spontaneous echocardiographic contrast formation in human blood', Circulation, 96, 889-896, 1997 https://doi.org/10.1161/01.CIR.96.3.889
  25. R. Rastegar, D. J. Harnick, P. Weidemann, V. Fuster, B. Coller, J. J. Badimon, J. Chesebro and M. E. Goldman, 'Spontaneous echo contrast videodensity is flow-related and is dependent on the relative concentrations of fibrinogen and red blood cells', J. Am. Coll. Cardiol. 41, 603-610, 2003 https://doi.org/10.1016/S0735-1097(02)02898-X
  26. X. Weng, G. Cloutier, R. Beaulieu and G. O. Roederer, 'Influence of acute-phase proteins on erythrocyte aggregation', Am. J. Physiol., 271, H2346-H2352, 1996
  27. M. Okumura, T. Fujinaga, K. Yamashita, N. Tsunoda and S. Mizuno, 'Isolation, characterization, and quantitative analysis of ceruloplasmin from horses', Am. J. Vet. Res., 52, 1979-1985, 1991
  28. F. M. Andrews, N. L. Korenek, W. L. Sanders and R. L. Hamlin, 'Viscosity and rheologic properties of blood from clinically normal horses', Am. J. Vet. Res., 53, 966-970, 1992
  29. M. W. Rampling, H. J. Meiselman, B. Neu and O. K. Baskurt, 'Influence of cell-specific factors on red blood cell aggregation', Biorheology, 41, 91-112, 2004