• 한국생물공학회:학술대회논문집
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• 2000.04a
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• pp.313-316
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• 2000

When 3 recombinant Chinese hamster ovary (rCHO) cell lines, CHO/dhfr-B-22-4, $CS13-1.00^{\ast}$ and $CSl3-0.02^{\ast}$, were cultivated in hyperosmolar media resulting from NaCl addition, their specific foreign protein productivity increased with medium osmolality. Glycine betaine was found to have a strong osmoprotective effect on all 3 rCHO cell lines. Inclusion of 15 mM glycine betaine in hyperosmolar medim enabled rCHO cell lines to grow at 557-573 mOsm/kg where they could not grow in the absence of glycine betaine. However, effect of glycine betaine inclusion in hyperomolar medium on foreign protein production differed among rCHO cell lines. CHO/dhfr-B22-4 cells retained enhanced specific human thrombopoietin (hTPO) productivity in the presence of glycine betaine, and thereby, the maximum hTPO titer obtained at 573 mOsm/kg was increased by 72% over that obtained in the control culture with physiological osmolality (292 mOsm/kg). On the other hand, enhanced specific antibody productivity of $CSl3-1.00^{\ast}$ and $CSl3-0.02^{\ast}$ at elevated osmolality decreased significantly in the presence of glycine betaine at a cost of the recovery of cell growth. As a result, the maximum antibody titer at 557 mOsm/kg was similar to that obtained in the control culture with physiological osmolality. Taken together, efficacy of the simultanous use of hyperosmotic pressure and glycine betaine as a means to improve foreign protein production was variable among different rCHO cell lines.

• Journal of Energy Engineering
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• v.11 no.2
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• pp.81-89
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• 2002

IGCC (Integrated Coal Gasification Combined Cycle) is a technology that generates electric power using coal gasification and gasified fuel. Carbon conversion value of IGCC is higher and the influence on the environment is lower than the pulverized coal power plant. Especially, in the nations where the weight of fossil fuel for power generation is remarkably high like in Korea, IGCC stands out as an alternative plan to cope with sudden limitation for the emissions. In this paper, system design study for the commercial IGCC system which the introduction is imminent to Korea was performed. Two cases of entrained gasification process are adapted, one is FHR(full heat recovery) type IGCC system for high efficiency and the other is Quench type IGCC system for low cost. System simulations using common codes like AspenPlus were performed for each system. In the case of Quench system, system option study and sensitivity analysis of the air extraction rate was performed. Thermal performance result for the FHR system is 42.6% (HHV, Net) and for the quench system is 40% (HHV, net) when 75% air is extracted.

• 한국신재생에너지학회:학술대회논문집
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• 2005.06a
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• pp.416-421
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• 2005

Most of solar system dissemination has been focused on domestic hot water system of which utilization to a building is relatively simple and safe than solar heating system. Through the survey on a cause of solar house dissemination failure in Korea, we conclude that design integration and systematic approach method for technology application are the most important element for a successful solar house. KIER(Korea Institute of Energy Research) and Hanbat National University have started new project on a development of Zero energy Solar House, called ZeSH which can be sustained just by natural energy without the support of existing fossil fuel. This is the 1st phase research of 10 years long-term ZeSH plan which develops a low-cost and $100\%$ self sufficient ZeSH. The goal of 1st phase ZeSH research is to get a $70\%$ self sufficiency only in thermal loads. Actual demonstration house, named KIER ZeSH I was designed and constructed as a result of 1st phase research work in the end of 2002. Various innovative technologies such as super insulation, high performance window, passive and active solar systems, ventilation heat recovery system are applied and evaluated to the KIER ZeSH I. A lot of computer simulations had been conducted for the optimal design and system integration in every design steps. Considering all the results from detailed hourly computer simulation, it is expected that at least $70\%$ self-sufficiency in thermal loads which is 1st phase target value can be excessively achieved in actual demonstration house. Besides, many valuable findings from the design and analysis to construction could be established such as collaboration method among the participants, practical design and construction techniques for system integration and the others. The purpose of this paper is to introduce the main findings through the development of KIER ZeSH I project. Practical guidelines in every design step for new low- or zero- energy solar house is proposed as result.

• Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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• v.22 no.4
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• pp.78-84
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• 2008

Inundation of substation and ground power equipment broke out every summer season in low-lying downtown and low-lying shore by localized heavy rain, typhoon and tidal wave. In case inundation excluding the exchanging cost of equipment, it occurs a great economic and social loss owing to recovery time and events of electric shock occur by inundation electrical facility. So we researched the installation situation of substation and power equipment and inundation loss at Flood Danger Area. And we analyzed refutation or law relating to the flood protection counterplan of US, England Australia. We present flood protection countermeasures by survey and analyzing the internal standard and his paper will be used to resent a reform proposal of electrical feinted law about flood protection.

• Korean Journal of Environmental Agriculture
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• v.32 no.2
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• pp.108-116
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• 2013

BACKGROUND: Certain crop-based waste materials have been recognized as cost-effective and highly efficient adsorbents for removal and recovery of different kind of heavy metals from aqueous solutions. The ability is strongly attributed to the carboxyl functional group of some pectin substances such as galacturonic acid often found in fruit peels. The present manuscript was aimed at assessing the potential applicability of banana peel for metal removal from contaminated waters. METHODS AND RESULTS: As revealed by laboratory investigations, banana peel contains pectin (10-21%), lignin (6-12%), cellulose (7.6-9.6%), and hemicelluloses (6.4-9.4%). The pectin extraction is reported to have glucose, galactose, arabinose, rhamnose, xylose, and galactouroninc acid. Several studies conducted under different conditions proved that banana peel is capable of adsorbing 5.71, 2.55, 28.00, 6.88, 7.97, and 5.80 mg/g of $Cd^{2+}$, $Co^{2+}$, $Cu^{2+}$, $Ni^{2+}$, $Pb^{2+}$, and $Zn^{2+}$, respectively, from aqueous solutions. Adsorption capacity is, however, dependent upon several factors including solution pH, dose of adsorbent and metal concentration, contact time and shaking speed. CONCLUSION(S): Since the annual world production of banana exceeds 100 million tons, about 40 million tons of banana peel (40% of total weight of the fresh fruit) remains vastly unused. Exploring a sound technology with banana peel would therefore, not only address the much needed sustainable tool for cleaning contaminated waters, but of course bring an additional value to the banana industry worldwide.

• Earthquakes and Structures
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• v.2 no.3
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• pp.233-256
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• 2011

This paper investigates the applicability of newly developed Cu-Al-Mn shape memory alloy (SMA) bars to retrofitting of historical masonry constructions by performing quasi-static tests of half-scale brick walls subjected to cyclic out-of-plane flexure. Problems associated with conventional steel reinforcing bars lie in pinching, or degradation of stiffness and strength under cyclic loading, and in their inability to restrain residual deformations in structures during and after intense earthquakes. This paper attempts to resolve the problems by applying newly developed Cu-Al-Mn SMA bars, characterized by large recovery strain, low material cost, and high machinability, as partial replacements for steel bars. Three types of brick wall specimens, unreinforced, steel reinforced, and SMA reinforced specimens are prepared. The specimens are subjected to quasi-static cyclic loading up to rotation angle enough to cause yielding of reinforcing bars. Corresponding nonlinear finite element models are developed to simulate the experimental observations. It was found from the experimental and numerical results that both the steel reinforced and SMA reinforced specimens showed substantial increment in strength and ductility as compared to the unreinforced specimen. The steel reinforced specimen showed pinching and significant residual elongation in reinforcing bars while the SMA reinforced specimen did not. Both the experimental and numerical observations demonstrate the superiority of Cu-Al-Mn SMA bars to conventional steel reinforcing bars in retrofitting historical masonry constructions.

• Membrane and Water Treatment
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• v.11 no.2
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• pp.97-109
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• 2020

Membrane fouling is the main drawback of membrane technology. Frequent membrane cleaning and membrane replacement are, therefore, required to reduce membrane fouling that causes permeate flux reduction, lower rejection, or higher operating pressure. Studies have proved that the alteration of membrane properties is the key controlling factor in lessening membrane fouling. Among stimuli-responsive membranes, thermo-responsive membrane is the most popular, with a drastic phase transition and swelling-shrinking behavior caused by the temperature change. In this study, the thermo-responsive ability of two commercial membranes, PolyCera® Titan membrane and PolyCera® Hydro membrane, at different temperatures was studied on the antifouling function of the membrane in palm oil mill effluent (POME) treatment. The evaluation of the membrane's thermo-responsive ability was done through three cycles of adsorption (fouling) and desorption (defouling) processes in a membrane filtration process. The experimental result depicted that PolyCera® Hydro membrane had a higher membrane permeability of 67.869 L/㎡.h.bar than PolyCera® Titan membrane at 46.011 L/㎡.h.bar. However, the high membrane permeability of PolyCera® Hydro membrane was compensated with low removal efficiency. PolyCera® Titan membrane with a smaller mean pore size had better rejection performance than PolyCera® Hydro membrane for all tested parameters. On the other hand, PolyCera® Titan membrane had a better hydrodynamic cleaning efficiency than PolyCera® Hydro membrane regardless of the hydrodynamic cleaning temperature. The best hydrodynamic cleaning performed by PolyCera® Titan membrane was at 35℃ with the flux recovery ratio (FRR) of 99.17 ± 1.43%. The excellent thermo-responsive properties of the PolyCera® Titan membrane could eventually reduce the frequency of membrane replacement and lessen the use of chemicals for membrane cleaning. This outstanding exploration helps to provide a solution to the chemical industry and membrane technology bottleneck, which is the membrane fouling, thus reducing the operating cost incurred by the membrane fouling.

• Journal of Microbiology and Biotechnology
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• v.23 no.5
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• pp.587-601
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• 2013

During the last few decades, it has become evident that the compatibility of the yeast biochemical environment with the ability to process and translate the RNA transcript, along with its capacity to modify a translated protein, are relevant requirements for selecting this host cell for protein expression in several pharmaceutical and clinical applications. In particular, Pichia pastoris is used as an industrial host for recombinant protein and metabolite production, showing a powerful capacity to meet required biomolecular target production levels in high-throughput assays for functional genomics and drug screening. In addition, there is a great advantage to using P. pastoris for protein secretion, even at high molecular weights, since the recovery and purification steps are simplified owing to relatively low levels of endogenous proteins in the extracellular medium. Clearly, no single microexpression system can provide all of the desired properties for human protein production. Moreover, chemical and physical bioprocess parameters, including culture medium formulation, temperature, pH, agitation, aeration rates, induction, and feeding strategies, can highly influence product yield and quality. In order to benefit from the currently available wide range of biosynthesis strategies using P. pastoris, this mini review focuses on the developments and technological fermentation achievements, providing both a comparative and an overall integration analysis. The main aim is to highlight the relevance and versatility of the P. pastoris biosystem to the design of more cost-effective microfactories to meet the increasing demands for recombinant membrane proteins and clinical antibodies for several therapeutic applications.

• Journal of Korean Society of Environmental Engineers
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• v.38 no.5
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• pp.219-227
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• 2016

As the largest source of food waste is housing, and the food waste properties are good enough to recycle, the proactive approach to conventional food waste disposal is highly regarded. This research is to examine food waste management in the Seoul Metropolitan Area (SMA) and non-SMA and to analyze the public inclination to disseminate zero-food waste system (ZFWS) with fermentation and extinction technology in multifamily housing estates. The self-administered questionnaire survey was conducted and the collected data were statistically analyzed. The main findings are summarized as follows: food waste in multifamily housing estates were retrieved by refuse truck and largely recycled for compost and forage. Also, many local governments were in favor of ZFWS, and unwilling to invest in it due to financial constraint. It's found that logistics of ZFWS is likely to be influenced by important features such as a considerable amount of cost saving, effective energy recovery, and efficient operation/management.

• Journal of Korean Neurosurgical Society
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• v.38 no.4
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• pp.281-286
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• 2005

Objective : The authors report our experience of urokinase thrombolysis in treating patients harboring nonaneurysmal spontanesous intraventricular hemorrhage[IVH] and evaluated complications, safety and feasibility of this procedure retrospectively. Methods : Fifty-three patients with nonaneurysmal IVH>15mL without underlying structural etiology or coagulopathy were recruited. The patients with Glasgow Coma Scale[GCS]<5 were excluded. A catheter was directed into the IVH. Hematoma aspiration was followed by instillation of urokinase at the ear level of drainage bag under intracranial pressure monitoring system. This was repeated every 6hours until half of its initial volume. For analysis of prognostic factors, we classified the patients into two groups by Glasgow outcome scale[GOS]; good [$GOS\;{\ge}3$] and bad [GOS<3] prognosis group, and performed comparative analysis between two groups. Results : Mean age was 60.2years. The baseline hematoma size ranged 16 to 72mL. IVH volume reduction was done by an average of 74.2%. As complications, there were 3cases of rebleeding and 2cases of ventriculitis. No intracranial adverse effects were observed during thrombolytic theraphy. At 6months after the procedure, 29patients had achieved a good recovery, 15remained vegetative. 9patients died in hospital. The main good prognostic factors were young age, small IVH volume, and high GCS. Conclusion : The results of this study suggest that this relatively easy and safe method of treatment will improve the prognosis. However, further clinical studies also must assess optimal thrombolytic dosage, frequency, and timing of urokinase instillation for safety and effectiveness and must include controlled comparisons of mortality, disability outcome, quality of life, time until convalescence, and cost of care in treated and untreated patients.