• 한국운동역학회지
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• 제30권1호
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• pp.73-81
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• 2020

Objective: The purpose of this study is to analyze the effects of aquatic walking exercise on gait and balance parameters of elderly women. Method: 15 elderly people were recruited for this study (age: 73.20±5.19 yrs, height: 153.87±3.36 cm, mass: 60.33±5.73 kg). All variables were measured using Gaitview AFA-50. The variables were the heel contact time ratio, gait angle, and M/P change ratio for gait patterns and ENV, REC, RMS, Total Length, TLC, Sway velocity, and Length/ENV for balance abilities. A paired t-test and the Wilcoxon signed-rank test were carried out to verify the differences in the test scores after participating in the water walking program. The significance level for all statistical analyses was set to α=.05. Results: As for the changes in their walking function after the exercise, heel contact time ratio (p<.01) showed a statistical significance, while gait angle and M/P change ratio did not reveal statistically significant differences. In the test of balance ability on both feet and with eyes opened, statistical significance was found in ENV, REC, RMS, TLC (p<.01), and sway velocity (p<.05), while the test with eyes closed showed statistical significance in length/ENV as well as ENV, REC, RMS, sway velocity (p<.01) TLC, and total length (p<.05). As for the single-leg stance balance ability, ENV and REC revealed statistically significant differences. Conclusion: These results show that water walking is effective for improving the function of the ankle flexor muscles, providing stability to the ankle joint during walking and helping efficient walk. In addition, it is also expected to help prevent falls due to loss of balance by improving the stability of lower extremity muscles and trunk.

• 한국자동차공학회논문집
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• 제12권5호
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• pp.1-11
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• 2004

In order to recognize thermal efficiency and power improvement in case that diesel cycle is turned into diesel-atkinson cycle, the fuel-air diesel-atkinson cycle considered gas exchange process is analyzed non-dimensionally and thermodynamically. As a result, in case of diesel-atkinson cycle, as expansion ratio is increased, thermal efficiency and mean effective pressure is increased and it has maximum value at Rec=1. When diesel cycle is turned into diesel-atkinson cycle by late intake valve closing timing, thermal efficiency and power is decreased because of the decline of effective compression ratio and intake airflow, but it could be compensated by increase of compression ratio or super-charged. In case compression ratio is compensated, Rec appears 1 around 100$^{\circ}$ ABDC, and it is expected that thermal efficiency is enhanced by 14.3% compared with conventional diesel cycle. In case compression ratio and intake airflow are compensated simultaneously, super-charged pressure is demanded 2.06bar at Rec=1 and it is more efficient when only compression ratio is compensated in the view point of thermal efficiency.

• 정보과학회 컴퓨팅의 실제 논문지
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• 제23권7호
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• pp.440-445
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• 2017

전자상거래 발전에 따라 온라인 쇼핑을 이용하는 사람들이 증가하였고 제품 또한 다양해지고 있다. 이러한 추세로 구매자가 만족할 수 있는 정확한 추천시스템의 중요성이 증대되었으며 정확도를 높이기 위한 새로운 방법의 연구가 계속되고 있다. 순환신경망은 시퀀스 학습에 적합한 딥 러닝 방법 중 하나이며 본 연구에서는 추천시스템의 정확도를 높이는 방법으로 구매자의 제품 접근순서를 순환신경망에 적용하여 알고리즘 성능평가를 하였다. 알고리즘 성능평가에는 대표적인 순환신경망 알고리즘과 최적화 알고리즘으로 진행하였다. 순환신경망 알고리즘으로는 RNN, LSTM, GRU 그리고 최적화 알고리즘으로는 Adagrad, RMSProp, Adam optimizer를 사용하였다. 실험 도구로는 구글의 오픈소스 라이브러리인 텐서플로우를 사용하였고 데이터는 RecSys Challenge 2015에서 제공하는 e-commerce session 데이터를 활용하였다. 실험 결과 실험 데이터에 적합한 최적의 하이퍼파라미터를 발굴하고 적용하여 RecSys Challenge 2015 참가자들의 결과와 비교하였다. 상품 접근 순서만을 학습시킨 결과이기 때문에 등수가 높지는 않았지만 기존 추천시스템에 접목한다면 정확도 향상에 기여할 수 있을 것으로 보인다.

• The Plant Pathology Journal
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• 제39권2호
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• pp.191-206
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• 2023

Ground cherry (Physalis pubescens) is the most prominent species in the Solanaceae family due to its nutritional content, and prospective health advantages. It is grown all over the world, but notably in northern China. In 2019 firstly bacterial leaf spot (BLS) disease was identified on P. pubescens in China that caused by both BLS pathogens Xanthomonas euvesicatoria pv. euvesicatoria resulted in substantial monetary losses. Here, we compared whole genome sequences of X. euvesicatoria to other Xanthomonas species that caused BLS diseases for high similarities and dissimilarities in genomic sequences through average nucleotide identity (ANI) and BLAST comparison. Molecular techniques and phylogenetic trees were adopted to detect X. euvesicatoria on P. pubescens using recQ, hrpB1, and hrpB2 genes for efficient and precise identification. For rapid molecular detection of X. euvesicatoria, loop-mediated isothermal amplification, polymerase chain reaction (PCR), and real-time PCR techniques were used. Whole genome comparison results showed that the genome of X. euvesicatoria was more closely relative to X. perforans than X. vesicatoria, and X. gardneri with 98%, 84%, and 86% ANI, respectively. All infected leaves of P. pubescens found positive amplification, and negative controls did not show amplification. The findings of evolutionary history revealed that isolated strains XeC10RQ, XeH9RQ, XeA10RQ, and XeB10RQ that originated from China were closely relative and highly homologous to the X. euvesicatoria. This research provides information to researchers on genomic variation in BLS pathogens, and further molecular evolution and identification of X. euvesicatoria using the unique target recQ gene through advance molecular approaches.

• KSII Transactions on Internet and Information Systems (TIIS)
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• 제12권5호
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• pp.2082-2102
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• 2018

In recent years, social network related applications such as WeChat, Facebook, Twitter and so on, have attracted hundreds of millions of people to share their experience, plan or organize, and attend social events with friends. In these operations, plenty of valuable information is accumulated, which makes an innovative approach to explore users' preference and overcome challenges in traditional recommender systems. Based on the study of the existing social network recommendation methods, we find there is an abundant information that can be incorporated into probability matrix factorization (PMF) model to handle challenges such as data sparsity in many recommender systems. Therefore, the research put forward a unified social network recommendation framework that combine tags, trust between users, ratings with PMF. The uniformed method is based on three existing recommendation models (SoRecUser, SoRecItem and SoRec), and the complexity analysis indicates that our approach has good effectiveness and can be applied to large-scale datasets. Furthermore, experimental results on publicly available Last.fm dataset show that our method outperforms the existing state-of-art social network recommendation approaches, measured by MAE and MRSE in different data sparse conditions.

• 생명과학회지
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• 제18권7호
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• pp.912-917
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• 2008

유전자재조합 hG-CSF의 생리활성을 분석하기 위하여 편상의 암세포로부터 분리되어진 cDNA를 이용하여 hG-CSF 유전자를 분리하여 동물세포(CHO cell lines)를 이용하여 재조합 단백질을 생산하였다. 재조합 단백질의 체내 생리활성을 분석하기 위하여0일과 2일에 피하주사 후 5일에 혈액을 채취하여 백혈구 수를 분석하였다. 투여 전과 비교하여 5일째에 백혈구 수는 현저하게 증가하였다. 또한, pEGFP-mUII-hG-CSF벡터를 소 태아로부터 분리되어진 체세포에 형질전환을 시켜서, EGFP signal을 나타내는 세포를 confocal를 이용하여 분리하여 수립하였다. 따라서, 이러한 결과는 유전자재조합 hG-CSF는 체내에서 강력한 생리활성을 나타내며, 또한 당쇄가 첨가되어지고 이중으로 연결되어진 새로운 돌연변이체를 포함하여 고 활성 재조합체의 생산이 가능할 것으로 보이며, pEGFP-mUII-hG-CSF벡터는 복제 형질전환 가축 생산을 위하여 유용하게 사용되어질 것으로 사료된다.

• 원자력산업
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• 제17권7호통권173호
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• pp.69-80
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• 1997

• 한국통신학회:학술대회논문집
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• 한국통신학회 1985년도 추계학술발표회 논문집
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• pp.224-228
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• 1985

• Animal cells and systems
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• 제3권4호
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• pp.413-415
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• 1999

Our previous report demonstrated that the rhp51$^＋$, a recA and RAD51 homolog of the fission yeast, encodes three transcripts of 1.9, 1.6 and 1.3 kb which have at least six polyadenylation sites. The 3'-end of the gene alone can direct the formation of multiple, discrete 3'ends of the transcripts. To identify the regulatory element required for the 3'-end formation of -rhp51$^＋$ deletion mapping analysis was performed. Northern blot analysis revealed that the 254-bp DNA fragment including 4 distinct poly (A) sites downstream from the Hindlll site, is crucial for normal 3'-end formation. Deletion of the 3'-terminal AU rich region caused appearance of read-through RNA, leading to enhancement of survival rate of the rhp51 deletion mutant in response to DNA damaging agent, methylmethane sulfonate (MMS). The results imply that the rhp51$^＋$ system may be useful for molecular analysis of the 3'-end formation of RNA in the fission yeast.

• Journal of Microbiology and Biotechnology
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• 제31권7호
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• pp.912-920
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• 2021

SOS response is a conserved response to DNA damage in prokaryotes and is negatively regulated by LexA protein, which recognizes specifically an "SOS-box" motif present in the promoter region of SOS genes. Myxococcus xanthus DK1622 possesses a lexA gene, and while the deletion of lexA had no significant effect on either bacterial morphology, UV-C resistance, or sporulation, it did delay growth. UV-C radiation resulted in 651 upregulated genes in M. xanthus, including the typical SOS genes lexA, recA, uvrA, recN and so on, mostly enriched in the pathways of DNA replication and repair, secondary metabolism, and signal transduction. The UV-irradiated lexA mutant also showed the induced expression of SOS genes and these SOS genes enriched into a similar pathway profile to that of wild-type strain. Without irradiation treatment, the absence of LexA enhanced the expression of 122 genes that were not enriched in any pathway. Further analysis of the promoter sequence revealed that in the 122 genes, only the promoters of recA2, lexA and an operon composed of three genes (pafB, pafC and cyaA) had SOS box sequence to which the LexA protein is bound directly. These results update our current understanding of SOS response in M. xanthus and show that UV induces more genes involved in secondary metabolism and signal transduction in addition to DNA replication and repair; and while the canonical LexA-dependent regulation on SOS response has shrunk, only 5 SOS genes are directly repressed by LexA.