The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Comparative Genomic Analysis and Rapid Molecular Detection of Xanthomonas euvesicatoria Using Unique ATP-Dependent DNA Helicase recQ, hrpB1, and hrpB2 Genes Isolated from Physalis pubescens in China

  • Faisal Siddique (College of Agriculture, Northeast Agricultural University) ;
  • Yang Mingxiu (College of Agriculture, Northeast Agricultural University) ;
  • Xu Xiaofeng (College of Agriculture, Northeast Agricultural University) ;
  • Ni Zhe (College of Agriculture, Northeast Agricultural University) ;
  • Haseeb Younis (College of Agriculture, Northeast Agricultural University) ;
  • Peng Lili (College of Agriculture, Northeast Agricultural University) ;
  • Zhang Junhua (College of Agriculture, Northeast Agricultural University)
  • Received : 2022.08.31
  • Accepted : 2023.03.07
  • Published : 2023.04.01
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Abstract

Ground cherry (Physalis pubescens) is the most prominent species in the Solanaceae family due to its nutritional content, and prospective health advantages. It is grown all over the world, but notably in northern China. In 2019 firstly bacterial leaf spot (BLS) disease was identified on P. pubescens in China that caused by both BLS pathogens Xanthomonas euvesicatoria pv. euvesicatoria resulted in substantial monetary losses. Here, we compared whole genome sequences of X. euvesicatoria to other Xanthomonas species that caused BLS diseases for high similarities and dissimilarities in genomic sequences through average nucleotide identity (ANI) and BLAST comparison. Molecular techniques and phylogenetic trees were adopted to detect X. euvesicatoria on P. pubescens using recQ, hrpB1, and hrpB2 genes for efficient and precise identification. For rapid molecular detection of X. euvesicatoria, loop-mediated isothermal amplification, polymerase chain reaction (PCR), and real-time PCR techniques were used. Whole genome comparison results showed that the genome of X. euvesicatoria was more closely relative to X. perforans than X. vesicatoria, and X. gardneri with 98%, 84%, and 86% ANI, respectively. All infected leaves of P. pubescens found positive amplification, and negative controls did not show amplification. The findings of evolutionary history revealed that isolated strains XeC10RQ, XeH9RQ, XeA10RQ, and XeB10RQ that originated from China were closely relative and highly homologous to the X. euvesicatoria. This research provides information to researchers on genomic variation in BLS pathogens, and further molecular evolution and identification of X. euvesicatoria using the unique target recQ gene through advance molecular approaches.

Keywords

Acknowledgement

We are especially thankful to Dr. Shuang Song (Assistance Professor, Northeast Agricultural University) for providing Xanthomonas euvesicatoria inoculum (Xeu1, Xeu2, and Xeu3). This research was funded by "Precision poverty alleviation project of planting industry science and technology, special project of central leading local science and technology development, grant number ZY18C08", "Isolation and identification of candidate genes related to response to the stress of Magnaporthe grisea in rice, nature fund project, Heilongjiang, China, grant number C2017032", and "Integration and extension of green control techniques for rice diseases in main rice production areas of Heilongjiang, grant number GA19B104".

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