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http://dx.doi.org/10.5352/JLS.2008.18.7.912

Biological Activity of Recombinant Human Granulocyte Colony-Stimulating Factor and Isolation of the Somatic Cell Transfected EGFP-hG-CSF Gene  

Park, Jong-Ju (Animal Biotechnology, The Graduate School of Bio. & Information Technology, Institute of Genetic Engineering, Hankyong National University)
Min, Kwan-Sik (Animal Biotechnology, The Graduate School of Bio. & Information Technology, Institute of Genetic Engineering, Hankyong National University)
Publication Information
Journal of Life Science / v.18, no.7, 2008 , pp. 912-917 More about this Journal
Abstract
To investigate the biological activity of recombinant human granulocyte colony-stimulating factor (rec-hG-CSF) in mammalian cells, hG-CSF gene was cloned using the eDNA extracted from the human squamous carcinoma cell lines and rec-hG-CSF was produced in CHO cell lines. To analyze the biological activity in vivo, the rec-hG-CSF protein was injected into mice subcutaneously on days 0 and 2. Blood was withdrawn for white blood cell (WBC) determination 5 days after the first injection. WBC values were found to have increased significantly. A pEGFP-mUII-hG-CSF vector was transfected into somatic cell lines isolated from bovine fetal cells. The colony expressing EGFP signals was observed with a confocal microscope. These data suggest that the rec-hG-CSF produced in this study has potent activity in vivo. Thus, the results of this biological activity show that rec-hG-CSF can be enhanced considerably by genetic engineering that affects potential activity, including mutations, which add the oligosaccharide chain and construct double-fusion proteins. A pEGFP-mUII-hG-CSF vector can be utilized for the production of cloned transgenic livestock.
Keywords
G-CSF; recombinant; biological activity; pEGFP-mUII-hG-CSF vector;
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